Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,554,356 T→C 100% N96D (AAC→GAC)  mraW ← S‑adenosyl‑methyltransferase MraW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,554,3560T→C100.0% 1503.8 401N96D (AAC→GAC) mraWS‑adenosyl‑methyltransferase MraW
Reads supporting (aligned to +/- strand):  new base (192/209):  ref base (0/0):  total (192/209)

Only 100 of 413 total aligned reads displayed.

CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453
                                                                                                  |                                                                                                 
cATCCGCATGTCCAAGCGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCa                                                                                                 <  2:2909523/101‑1 (MQ=255)
  tCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGc                                                                                               <  1:1055195/101‑1 (MQ=255)
    cGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGccc                                                                                             <  1:3752386/101‑1 (MQ=255)
     gCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCt                                                                                            >  1:4480189/1‑101 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           <  2:3798290/101‑1 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          >  2:8864145/1‑101 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          >  2:2039208/1‑101 (MQ=255)
        tGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCg                                                                                         >  2:4785482/1‑101 (MQ=255)
         gTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        >  2:1924310/1‑101 (MQ=255)
         gTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        >  1:7558756/1‑101 (MQ=255)
           ccAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgc                                                                                      <  1:266202/101‑1 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    <  2:6808562/101‑1 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    >  1:3222974/1‑101 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    >  2:4559263/1‑101 (MQ=255)
              aGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGcgcg                                                                                   >  2:3678664/1‑101 (MQ=255)
              aGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGcgcg                                                                                   >  1:9172194/1‑101 (MQ=255)
               gggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGa                                                                                  <  2:1633631/101‑1 (MQ=255)
               gggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGa                                                                                  >  2:8354795/1‑101 (MQ=255)
                 gCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtc                                                                                <  1:2577500/101‑1 (MQ=255)
                  cccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtct                                                                               <  1:7093804/101‑1 (MQ=255)
                  cccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtct                                                                               <  2:7563211/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              >  2:721101/1‑101 (MQ=255)
                     gTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCAGCACCCCGTCCAGCCCTTCGGGCGCGATCTcgg                                                                            >  1:4381193/1‑101 (MQ=255)
                     gTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcgg                                                                            <  2:2794001/101‑1 (MQ=255)
                      tCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggc                                                                           <  2:2386117/101‑1 (MQ=255)
                        gcgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcgg                                                                         >  2:6101647/1‑101 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGa                                                                      >  1:6062852/1‑101 (MQ=255)
                            aGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     <  2:753083/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  1:3013705/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCCTCGGGCGCGATCTCGGCGGCGACg                                                                    <  2:4377241/101‑1 (MQ=255)
                              caaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcgtagc                                                                   >  2:7111011/2‑97 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  <  1:949294/101‑1 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  >  1:3413120/1‑101 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  >  1:3897280/1‑101 (MQ=255)
                                atgAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCa                                                                 <  2:6275044/99‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               >  1:1296295/1‑101 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              >  2:6391836/1‑101 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              >  2:3221357/1‑101 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             >  2:4910518/1‑101 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             >  2:459494/1‑101 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            >  1:6659058/1‑101 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         <  1:9022883/101‑1 (MQ=255)
                                         cgcgCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTc                                                        <  1:7299223/101‑1 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       >  1:5095220/1‑101 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       <  1:560685/101‑1 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       <  2:224875/101‑1 (MQ=255)
                                           cgctgcgCCTGATCGACCTGCCTCGACGACACGCCCAGGTCCAGCCTCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      <  1:5911307/95‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     >  2:5348011/1‑101 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     <  2:7928690/101‑1 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaa                                                    <  2:3897165/101‑1 (MQ=255)
                                               cGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAg                                                  <  1:4830335/101‑1 (MQ=255)
                                                ggCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAgg                                                 >  1:1815485/1‑101 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              <  2:7383971/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             >  2:8106791/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             >  1:5237977/1‑101 (MQ=255)
                                                     gATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            <  1:1740747/101‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  1:7430232/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         >  2:500013/1‑101 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         >  1:2068261/1‑101 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  1:1939184/101‑1 (MQ=255)
                                                         gAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGa                                        <  1:6233547/101‑1 (MQ=255)
                                                         gAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGa                                        <  1:3678773/101‑1 (MQ=255)
                                                            cTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGacca                                     >  1:3559177/1‑101 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    >  1:2748156/1‑101 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCATCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGCGGTTCGAAAAGGTGTTCTCGaccag                                    >  1:4094925/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  1:825556/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  2:5236464/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  1:6405052/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAGCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGAGTTCTCGcccaga                                   >  2:4223660/1‑100 (MQ=255)
                                                                ctCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 <  2:4286669/100‑1 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 >  2:5863040/1‑101 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCAACGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 >  1:2861692/1‑101 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  1:4573070/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  2:7429935/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  1:1200741/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGAGTTCTCGaccagcacca                               >  2:5549242/1‑101 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             <  1:1545922/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  2:7733343/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:7879531/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:6829795/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:2483703/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           >  1:4155855/1‑101 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           >  2:343755/1‑101 (MQ=255)
                                                                       acacGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGc                          <  1:5713333/101‑1 (MQ=255)
                                                                       acacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGc                          <  2:1167016/101‑1 (MQ=255)
                                                                       acacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGc                          >  2:1486616/1‑101 (MQ=255)
                                                                         acGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTc                        <  2:8996702/101‑1 (MQ=255)
                                                                          cGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCg                       <  2:866974/101‑1 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  1:4957524/1‑101 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  2:9100851/1‑101 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               >  2:8602827/1‑101 (MQ=255)
                                                                                   ccAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcg              >  1:3294387/1‑101 (MQ=255)
                                                                                     aGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgcc            >  1:4633950/1‑101 (MQ=255)
                                                                                          caccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGcc       >  1:6974790/1‑101 (MQ=255)
                                                                                          caccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGcc       <  2:6900290/101‑1 (MQ=255)
                                                                                           cccccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      <  1:8642009/100‑1 (MQ=255)
                                                                                           cccccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      <  1:8975254/100‑1 (MQ=255)
                                                                                            ccccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAg     <  1:7983288/101‑1 (MQ=255)
                                                                                              ccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAggg   <  1:6101884/101‑1 (MQ=255)
                                                                                               cGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGgc  <  2:6681337/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG