BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,555	C→T	100%	intergenic (+27/+167)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,555	0	C→T	100.0%	1175.9	408	intergenic (+27/+167)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (105/300): ref base (0/1): total (106/302)

Only 100 of 444 total aligned reads displayed.


CTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.G.GG.GGCGCTGCCCCC.CTC.CT.G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTT  >  NC_014034/2566456‑2566649


      gATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGCCATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.ATC.TT.ggcct                                                                                            <  1:8291003/101‑5 (MQ=255)


       gtGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.ggcctt                                                                                           <  2:388138/100‑6 (MQ=38)


       aTGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCTCTGAGACGAAAAG.G.GG.GGGGGTGCCCCc.ccc.cttgccct                                                                                            >  2:6929407/1‑95 (MQ=255)


        tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.ggccttc                                                                                          <  2:1512839/101‑7 (MQ=38)


        tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.ggccttc                                                                                          <  1:3148110/101‑7 (MQ=38)


            cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGcc                                                                                      <  1:6654910/101‑1 (MQ=37)


            cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGcc                                                                                      >  1:3595548/1‑101 (MQ=37)


             gATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCa                                                                                     <  2:8917344/101‑1 (MQ=37)


                 cGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTc                                                                                 <  1:2977872/101‑1 (MQ=37)


                  gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCa                                                                                <  2:7814776/101‑1 (MQ=37)


                    tCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCAcc                                                                              <  2:7001388/101‑1 (MQ=37)


                    tCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCAcc                                                                              <  1:193020/101‑1 (MQ=37)


                        cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCAcccccc                                                                          <  2:7137727/101‑1 (MQ=25)


                        cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCAcccccc                                                                          >  2:5871235/1‑101 (MQ=25)


                         tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCg                                                                         <  1:6541963/101‑1 (MQ=25)


                           gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCCATTCCCCCCCCcgg                                                                       >  2:1389845/1‑101 (MQ=25)


                           gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCggg                                                                       <  1:4101714/101‑1 (MQ=25)


                             ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGat                                                                     <  2:1062172/101‑1 (MQ=25)


                             ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGat                                                                     <  1:2185563/101‑1 (MQ=25)


                              cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGata                                                                    <  2:2617998/101‑1 (MQ=25)


                               gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCCATTCACCCCCCGGGatat                                                                   >  1:525409/1‑101 (MQ=25)


                                 ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  1:6705999/101‑1 (MQ=21)


                                 ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  2:848735/101‑1 (MQ=21)


                                 ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  1:1822618/101‑1 (MQ=21)


                                  gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTc                                                                <  1:5487580/101‑1 (MQ=21)


                                   ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCg                                                               <  2:2538970/101‑1 (MQ=21)


                                    gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCgg                                                              <  2:3133003/101‑1 (MQ=21)


                                     ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCggg                                                             <  2:3590254/101‑1 (MQ=21)


                                      tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  1:6882056/101‑1 (MQ=21)


                                      tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  1:5785720/101‑1 (MQ=21)


                                      tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  2:3456355/101‑1 (MQ=21)


                                       cTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCa                                                           <  2:2350575/101‑1 (MQ=21)


                                       cTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CCC.TT.GGCCCTCGGCCCACTCACCCCCCCGGAGATTTCGGGCa                                                           >  1:7063049/1‑101 (MQ=21)


                                           cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                                       <  1:4597723/101‑1 (MQ=21)


                                            gccgcTCCAGCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  1:1676109/101‑1 (MQ=18)


                                            gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  1:9151629/101‑1 (MQ=21)


                                             ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                     <  2:3706003/101‑1 (MQ=21)


                                              cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                                    <  2:6339159/101‑1 (MQ=21)


                                              cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                                    <  1:2321962/101‑1 (MQ=21)


                                               gcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGGTAaag                                                   >  1:1039313/1‑101 (MQ=21)


                                               gcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaag                                                   <  1:869769/101‑1 (MQ=21)


                                                cgACATCGGCTGGCGCGCGCTCTGAGACG.AACC.G.GGTGGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                  <  1:8155435/101‑1 (MQ=21)


                                                cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                  >  1:1213652/1‑101 (MQ=21)


                                                 gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCCATTCACCCCCCGGGATATTTCGGGCAAGATAaagaa                                                 >  2:4578322/1‑101 (MQ=21)


                                                 gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaagaa                                                 <  1:8556214/101‑1 (MQ=21)


                                                   cATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                               <  2:6521144/101‑1 (MQ=21)


                                                    aTCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGGGGGGCCCCC.CTC.TTCGC.CTTCCGCCCATTCACCCCCCCGGGGATTTTTGGCCAGATACAGAAAGt                                              >  2:4047223/1‑101 (MQ=255)


                                                      cGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                            >  1:5101828/1‑101 (MQ=21)


                                                      cGGCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                            >  1:2417895/1‑101 (MQ=21)


                                                       ggCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                           >  1:7936868/1‑101 (MQ=21)


                                                        gCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                          <  1:1994603/101‑1 (MQ=21)


                                                        gCTGGCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                          <  2:2225602/101‑1 (MQ=21)


                                                         cTGGCGCGCGCTCTGAGACG.GAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCcttt                                         <  1:7201113/101‑1 (MQ=18)


                                                           ggCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                       >  2:3295937/1‑101 (MQ=21)


                                                           ggCGCGCGCTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                       <  1:2649652/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:2198703/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:7303657/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:9046709/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:7865305/101‑1 (MQ=21)


                                                                gcgcTCTGAGCCG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                  <  2:6132497/101‑1 (MQ=21)


                                                                gcgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                  <  2:8246253/101‑1 (MQ=21)


                                                                 cgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                 <  2:5288098/101‑1 (MQ=21)


                                                                 cgcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                 <  2:1856942/101‑1 (MQ=21)


                                                                  gcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                >  1:6396003/1‑101 (MQ=21)


                                                                  gcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                <  2:4961700/101‑1 (MQ=21)


                                                                  gcTCTGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                <  1:8201249/101‑1 (MQ=21)


                                                                   ctctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                               >  1:531514/1‑101 (MQ=21)


                                                                   ctctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCACCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                               <  1:9034448/101‑1 (MQ=21)


                                                                    tctGAGACG.AACCGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:2925057/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:5538083/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              >  1:2786357/1‑101 (MQ=21)


                                                                    tctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:8056132/101‑1 (MQ=21)


                                                                     ctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             <  2:3944920/101‑1 (MQ=21)


                                                                     ctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             >  2:7561907/1‑101 (MQ=21)


                                                                     ctGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             <  2:6661388/101‑1 (MQ=21)


                                                                     ctGAGACG.AAAC.GTGG.GGCGCTGCCCCC.CTC.TT.GGCCTGCGGCCAATTCACCCCCCGGGCAATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATcctc                             >  2:5685894/1‑98 (MQ=18)


                                                                     ccgatctg.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             <  1:4814905/94‑1 (MQ=18)


                                                                      tGAGACG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                            <  2:4347223/101‑1 (MQ=21)


                                                                       gagaCG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGTCTTCGGCCAATTCACCCACCCGGATATTTAGGGCAAGATGAAGAAGGACTTTTCATTTCATCTTGcc                           >  1:3982720/1‑101 (MQ=18)


                                                                         gaCG.AAACGG.GG.GGCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                         <  2:1758981/101‑1 (MQ=21)


                                                                              aaacgg.gg.ggCGCTTCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                     <  1:7452475/96‑1 (MQ=21)


                                                                               aacgg.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                    <  1:5616005/97‑1 (MQ=25)


                                                                                gcag.gg.ggCGCTG.CCCC.CTC.TT.GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggg             >  2:3238347/4‑101 (MQ=0)


                                                                                 cgg.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc                  >  1:7314422/3‑101 (MQ=25)


                                                                                 cag.gg.ggCGCTG.CCCC.CTC.TT.GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg            >  2:2476428/3‑101 (MQ=0)


                                                                                  gg.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTTGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTCCCCGAAATATcc                 >  1:6293486/2‑101 (MQ=21)


                                                                                  gg.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                 >  1:3739418/2‑101 (MQ=25)


                                                                                  gg.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                 >  2:2802692/2‑101 (MQ=25)


                                                                                  ag.gg.ggCGCTGCCCCCTCTT.GG.C..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg           >  2:2060547/2‑101 (MQ=0)


                                                                                   g.gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                <  1:6099455/101‑1 (MQ=25)


                                                                                     gg.ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg               >  2:465830/1‑101 (MQ=25)


                                                                                        ggCGCTGCCCCCTCTT.GG.C..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc       <  2:7410274/101‑5 (MQ=2)


                                                                                        ggCGCTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggg             >  1:3284383/1‑101 (MQ=37)


                                                                                         gcgcTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgggg            <  1:4373604/101‑1 (MQ=37)


                                                                                          cgcTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggggg           >  1:7655364/1‑101 (MQ=37)


                                                                                           gcTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa          <  1:8933065/101‑1 (MQ=37)


                                                                                            cTGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg         <  1:1389877/101‑1 (MQ=37)


                                                                                             tGCCCCC.CTCTCG.GC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc        <  2:870211/101‑1 (MQ=37)


                                                                                             tGCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc        >  1:4737868/1‑101 (MQ=37)


                                                                                              gCCCCC.CTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGcc       >  1:8062860/1‑101 (MQ=37)


CTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.G.GG.GGCGCTGCCCCC.CTC.CT.G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTT  >  NC_014034/2566456‑2566649

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG