Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,579,493 A→C 100% S227A (TCC→GCC)  pgi ← glucose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,579,4930A→C100.0% 1472.2 400S227A (TCC→GCC) pgiglucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  new base (187/212):  ref base (0/0):  total (188/212)

Only 100 of 418 total aligned reads displayed.

GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590
                                                                                                  |                                                                                                 
gctcGAATAGCGCCCGTCCTTCCAAGTCTCGATGCTGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCCGTCGAGATTGCGgcga                                                                                                 <  2:9112190/98‑1 (MQ=255)
gACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGgcga                                                                                                 >  1:2650246/1‑101 (MQ=255)
  ccGAAGAGCGTCCGCCGCCCGAATCCTAGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCCCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAt                                                                                               <  2:2247630/101‑1 (MQ=255)
   cGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAtt                                                                                              >  1:4633949/1‑101 (MQ=255)
   cGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAtt                                                                                              >  2:4922109/1‑101 (MQ=255)
   cGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTAGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAtt                                                                                              <  1:8294035/101‑1 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             >  1:2306347/1‑101 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             <  2:656760/101‑1 (MQ=255)
    gAATAGCGCCCGACCACCCAATCCTCGAGGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGGCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             >  1:6520802/1‑101 (MQ=255)
     aaTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgc                                                                                            >  1:2880955/1‑101 (MQ=255)
     aaTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgc                                                                                            >  1:3353753/1‑101 (MQ=255)
      aTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcg                                                                                           >  2:5646429/1‑101 (MQ=255)
      aTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcg                                                                                           >  1:4608386/1‑101 (MQ=255)
      aTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcg                                                                                           <  1:5089165/101‑1 (MQ=255)
        aGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgcc                                                                                         >  1:2466812/1‑101 (MQ=255)
        aGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgcc                                                                                         >  2:3691823/1‑101 (MQ=255)
          cgcccgccCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgccgc                                                                                       <  2:2298873/101‑1 (MQ=255)
             ccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCgg                                                                                    <  2:4961275/101‑1 (MQ=255)
             ccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCgg                                                                                    >  2:1950666/1‑101 (MQ=255)
              cgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCggg                                                                                   >  2:8314331/1‑101 (MQ=255)
                cccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTc                                                                                 >  2:1469273/1‑101 (MQ=255)
                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  2:1227177/101‑1 (MQ=255)
                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  2:7432840/101‑1 (MQ=255)
                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  1:4089532/101‑1 (MQ=255)
                  cacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCg                                                                               <  2:416683/101‑1 (MQ=255)
                   acccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGt                                                                              >  2:8677263/1‑101 (MQ=255)
                    cccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTg                                                                             <  2:8511873/101‑1 (MQ=255)
                     ccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGa                                                                            >  1:4728963/1‑101 (MQ=255)
                      caATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAc                                                                           >  1:2011292/1‑101 (MQ=255)
                      caATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAc                                                                           >  1:7595908/1‑101 (MQ=255)
                      caATCCGCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAc                                                                           <  2:5204148/101‑1 (MQ=255)
                         tCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCtt                                                                        <  2:4424689/101‑1 (MQ=255)
                         gccTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCtt                                                                        <  2:6656075/100‑1 (MQ=255)
                         accTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCtt                                                                        <  2:4608251/100‑1 (MQ=255)
                           cTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcg                                                                      >  2:5402647/1‑101 (MQ=255)
                              gAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCTCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccg                                                                   >  1:7463309/1‑101 (MQ=255)
                                    gAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATcc                                                             >  1:9079504/1‑101 (MQ=255)
                                    gAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATcc                                                             <  1:6019953/101‑1 (MQ=255)
                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            >  2:477578/1‑101 (MQ=255)
                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            <  2:1588151/101‑1 (MQ=255)
                                      aGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAg                                                           >  1:6864960/1‑101 (MQ=255)
                                       gACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAgg                                                          <  1:3929356/101‑1 (MQ=255)
                                          gcgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgcgc                                                       >  2:7591791/1‑101 (MQ=255)
                                           cgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGcgcg                                                      >  1:4516781/1‑101 (MQ=255)
                                              ccgggcgggATGCCGAATGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggc                                                   <  1:5256410/100‑1 (MQ=255)
                                                   ggggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                              <  1:2545321/100‑1 (MQ=255)
                                                    gggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                             >  2:3530031/1‑101 (MQ=255)
                                                    gggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTACGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                             >  1:8131949/1‑101 (MQ=255)
                                                     ggAGGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                            <  1:8506536/101‑1 (MQ=255)
                                                        tGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCa                                         <  1:8144746/101‑1 (MQ=255)
                                                         gCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAt                                        >  2:8066093/1‑101 (MQ=255)
                                                          ccGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAtt                                       >  1:6432749/1‑101 (MQ=255)
                                                          ccGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAtt                                       <  1:553360/101‑1 (MQ=255)
                                                          ccGAAAGCCGCCGTCCTGGCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAtt                                       <  1:1733371/101‑1 (MQ=255)
                                                            gAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTgg                                     >  1:3083634/1‑101 (MQ=255)
                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    >  1:8741488/1‑101 (MQ=255)
                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    >  1:4923349/1‑101 (MQ=255)
                                                              aaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTc                                   <  2:8152269/101‑1 (MQ=255)
                                                                gccgccGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCAt                                 <  2:3078434/101‑1 (MQ=255)
                                                                   gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGtgagggt                              >  2:2599459/1‑95 (MQ=255)
                                                                     cgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtct                            <  2:7971587/101‑1 (MQ=255)
                                                                     cgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtct                            <  2:6046097/101‑1 (MQ=255)
                                                                     cgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtct                            <  1:7165319/101‑1 (MQ=255)
                                                                     cgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtct                            <  1:1498240/101‑1 (MQ=255)
                                                                      gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                           >  2:1125170/1‑101 (MQ=255)
                                                                      gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                           >  1:8430692/1‑101 (MQ=255)
                                                                      gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                           >  2:3897178/1‑101 (MQ=255)
                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          <  2:3999761/101‑1 (MQ=255)
                                                                        ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATGGTCTCGa                         >  2:6236403/1‑101 (MQ=255)
                                                                        ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                         >  2:3359134/1‑101 (MQ=255)
                                                                         cTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGAt                        >  2:7229139/1‑101 (MQ=255)
                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       >  1:5890892/1‑101 (MQ=255)
                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       <  1:3581348/101‑1 (MQ=255)
                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      <  2:7006262/101‑1 (MQ=255)
                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      <  2:8878907/101‑1 (MQ=255)
                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      <  2:3840606/101‑1 (MQ=255)
                                                                            tcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgt                     <  1:7980529/101‑1 (MQ=255)
                                                                             cggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtg                    <  2:1791224/101‑1 (MQ=255)
                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   >  1:405675/1‑101 (MQ=255)
                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   >  1:976311/1‑101 (MQ=255)
                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   >  2:6327364/1‑101 (MQ=255)
                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   >  1:4370251/1‑101 (MQ=255)
                                                                               gtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggt                  <  1:7063510/101‑1 (MQ=255)
                                                                                tcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggtg                 <  1:1417649/101‑1 (MQ=255)
                                                                                 cgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCCTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGa                <  1:6684572/101‑1 (MQ=255)
                                                                                  gcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGaa               <  1:7275791/101‑1 (MQ=255)
                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              >  2:2141409/1‑101 (MQ=255)
                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              <  1:1940518/101‑1 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             >  1:1025690/1‑101 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             <  2:5792/101‑1 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             >  2:2667153/1‑101 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGGGAAgg             >  2:3219535/1‑101 (MQ=255)
                                                                                     gtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGt            <  2:2880857/101‑1 (MQ=255)
                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCtt         <  1:3771265/101‑1 (MQ=255)
                                                                                          gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg       <  2:7772133/101‑1 (MQ=255)
                                                                                          gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg       >  2:2374342/1‑101 (MQ=255)
                                                                                          gaTCGCGGCGAATGGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg       <  1:6219840/101‑1 (MQ=255)
                                                                                            tCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGAc     <  2:4657370/101‑1 (MQ=255)
                                                                                             cgcgGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGCCg    >  2:3332498/1‑101 (MQ=255)
                                                                                               cgGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGcc  <  1:2826874/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG