Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,590,068 C→G 100% V12L (GTT→CTT)  glnE ← glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,590,0680C→G100.0% 1499.5 402V12L (GTT→CTT) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (216/185):  ref base (0/0):  total (217/185)

Only 100 of 419 total aligned reads displayed.

CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165
                                                                                                  |                                                                                                 
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               >  2:7800372/1‑101 (MQ=255)
   aCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCg                                                                                              <  1:2459471/101‑1 (MQ=255)
    cAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             >  1:7954471/1‑101 (MQ=255)
    cAGCCCGCCGGGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             <  1:5290951/101‑1 (MQ=255)
      gCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGc                                                                                           <  2:4125957/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  2:8788752/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  1:5721547/101‑1 (MQ=255)
          gccgTGCCCGCGCTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGt                                                                                       >  1:6569498/1‑101 (MQ=255)
          gccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGt                                                                                       <  2:417182/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  2:5668168/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    >  1:1015695/1‑101 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTAAg                                                                                    <  1:4786657/101‑1 (MQ=255)
               gCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGc                                                                                  >  2:1508344/1‑101 (MQ=255)
                   gcgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGa                                                                              <  2:8610448/101‑1 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            >  1:6285461/1‑101 (MQ=255)
                      aTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           <  1:2763072/101‑1 (MQ=255)
                       tCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCa                                                                          >  1:5083986/1‑101 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  2:114812/1‑101 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  1:8679573/1‑101 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         <  2:6190245/101‑1 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         <  1:7635632/101‑1 (MQ=255)
                          aCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAgg                                                                       >  2:4721349/1‑101 (MQ=255)
                           cccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGc                                                                      <  2:5035818/101‑1 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTc                                                                    >  1:5806703/1‑101 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 >  1:8491224/1‑101 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 >  1:7327382/1‑101 (MQ=255)
                                 gATTTCGGGCGGCAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAA.GGGCGGGCGGGGCAGGGGGGGAGCAGAGCTCAAGgc                                                               >  1:8105662/1‑101 (MQ=255)
                                 gATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATgg                                                                >  2:6635647/1‑101 (MQ=255)
                                  aTTTCGGGCGGAAAATCNCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               <  2:4050957/101‑1 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               <  1:140409/101‑1 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               >  2:1350129/1‑101 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               <  1:2981085/101‑1 (MQ=255)
                                    ttCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgcgc                                                             >  2:7099734/1‑101 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  1:2586957/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  2:6254588/1‑101 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  2:2444498/1‑101 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  1:8077/1‑101 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          >  2:547704/1‑101 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          <  1:7982666/101‑1 (MQ=255)
                                        ggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGa                                                         >  1:4871332/1‑101 (MQ=255)
                                         gCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGAc                                                        >  2:8457324/1‑101 (MQ=255)
                                          cGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACt                                                       <  2:8303716/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  2:6390926/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  2:2319980/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  1:6053606/101‑1 (MQ=255)
                                             aaaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGGGCCAGACTAgc                                                    >  1:3841003/1‑101 (MQ=255)
                                                aTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccgc                                                 >  1:4408556/1‑101 (MQ=255)
                                                aTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccgc                                                 >  2:7650449/1‑101 (MQ=255)
                                                aTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccgc                                                 >  1:7378615/1‑101 (MQ=255)
                                                aTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccgc                                                 >  2:6530233/1‑101 (MQ=255)
                                                   gCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCcgccgc                                              >  2:3886257/1‑101 (MQ=255)
                                                   gCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCcgccgc                                              <  1:6972308/101‑1 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           <  1:3169557/101‑1 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           >  2:3429772/1‑101 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           <  1:333672/101‑1 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           >  2:1790483/1‑101 (MQ=255)
                                                       tAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGc                                          <  1:6733508/101‑1 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         >  2:7119037/1‑101 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         >  1:3716851/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        >  1:8330038/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        <  2:4871172/101‑1 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        <  1:1109690/101‑1 (MQ=255)
                                                           gggCAACGACATGGGGGCCAGGGGCCGGATCAAGGGGAGGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCg                                      <  1:2204557/101‑1 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     >  2:3192381/1‑101 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGGCAGGCGGGAGGCAGGGCTGAGGGCGCCAGGCGAGCCCCACCAAGCGCCgg                                     >  2:2927277/1‑101 (MQ=255)
                                                              cAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGt                                   >  2:7983587/1‑101 (MQ=255)
                                                               aatgACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  2:226828/4‑101 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  1:4113530/1‑101 (MQ=255)
                                                                 cGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCaa                                <  1:4616933/101‑1 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               >  1:4029364/1‑101 (MQ=255)
                                                                   gcATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAgg                              <  1:928969/100‑1 (MQ=255)
                                                                   aCATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAgg                              <  1:1786921/101‑1 (MQ=255)
                                                                    cATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgc                             >  1:6407431/1‑101 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            <  1:3609652/101‑1 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            >  1:7715256/1‑101 (MQ=255)
                                                                           gcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaaa                      >  2:6749951/1‑101 (MQ=255)
                                                                              aCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgc                   >  2:5304066/1‑101 (MQ=255)
                                                                              aCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgc                   <  2:1950632/101‑1 (MQ=255)
                                                                               cGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcg                  <  1:7559223/101‑2 (MQ=255)
                                                                                gggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccc                 <  1:7464354/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAGGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:896139/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                >  1:3899874/1‑101 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  2:4526093/101‑1 (MQ=255)
                                                                                  gcgggATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccg               <  2:26912/98‑1 (MQ=255)
                                                                                   ccGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgc              <  1:5916977/101‑1 (MQ=255)
                                                                                      gATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcccc           >  1:9078566/1‑101 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGACGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          <  2:8075618/101‑1 (MQ=255)
                                                                                        tCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTg         <  2:3766877/101‑1 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        <  1:7115447/101‑1 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        >  2:4625869/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  2:7237999/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  1:1558003/1‑101 (MQ=255)
                                                                                           aGGGGAAGGGGCGGGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGGCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCTCAAATGCCCCGCCCCTGTCa      <  2:1683954/101‑1 (MQ=255)
                                                                                             gggAAGGGGCGTGCGGGTCAGGCGGGATGCAGGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATc    <  2:8679188/101‑1 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   >  1:3241063/1‑101 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   <  2:5163606/101‑1 (MQ=255)
                                                                                              ggAAGGGGCGGGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   <  1:5619183/101‑1 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  >  1:4262117/1‑101 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  <  1:1239134/101‑1 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGAGGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  <  2:7378291/101‑1 (MQ=255)
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CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG