Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	70.6	19	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (1/18):  ref base (0/0):  total (1/18)

GCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCG  >  NC_014034/2602953‑2603139                                                                                                  |                                                                                          cggggcgggCCGCCCGCGGCGGGCGGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGAc                                                                                        <  2:177967/99‑1 (MQ=255)  gtggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGTCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACg                                                                                       <  1:5001888/99‑1 (MQ=255)  ggggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACg                                                                                       <  2:8137412/100‑1 (MQ=255)  ggggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACg                                                                                       <  1:6169308/100‑1 (MQ=255)   gggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGc                                                                                      <  1:5855391/101‑1 (MQ=255)     gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                    <  1:4872202/101‑1 (MQ=255)      gggggCGGCCGCCGGGGGCAGCGAGCGGATCGCCATCCCCTCGCTGGGGGGGGCCGCGATCGCCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcg                                                                                   <  1:2943169/100‑1 (MQ=255)      gggggCCGGCGCGGCGGGCGGCGAGCGGACCGACATCCCCTCGCGGGCGGGGGCCGCGATCGCCCGCGCCGCCGCCCCCCCGCCGCCCGCCGCGACGCCcg                                                                                   <  2:7628286/100‑1 (MQ=255)      cggtGCTGCCGCAGAGGGAAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCGGCCGTCACCCAGCCGCCCGCCGCGACGCCcg                                                                                   <  2:1655391/101‑1 (MQ=255)      cgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcg                                                                                   <  1:5657196/101‑1 (MQ=255)      cgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcg                                                                                   <  1:3715370/101‑1 (MQ=255)         ggCGGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCgg                                                                                <  2:4348075/101‑1 (MQ=255)          gcggccgccGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGc                                                                               <  2:7394727/98‑1 (MQ=255)             gccgccGCGGTCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGcccc                                                                            <  1:8112333/101‑1 (MQ=255)              ccgccgTGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccccc                                                                           <  1:2106563/101‑1 (MQ=255)                 ccgcGGGCAGGGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGCCCCCCAGCCCCCCGCCGCGACGCCCGCGGCCCCCGcc                                                                        <  2:1228793/101‑1 (MQ=255)                      ggCAGCGAGCGGATCGACATCCCCTCGCGGGGGGGGGCCGCGATCGCCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                   <  2:4095604/101‑1 (MQ=255)                                                                      cgcgCCCCCGCCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgccgc                   <  1:2228301/101‑1 (MQ=255)                                                                                       gccgccCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCATCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCCgcg  >  1:7386579/1‑101 (MQ=255)                                                                                                  |                                                                                          GCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCG  >  NC_014034/2602953‑2603139 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 37 ≤  ATCG