Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,193 A→G 100% intergenic (‑169/+107) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1930A→G100.0% 1407.4 430intergenic (‑169/+107)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (178/252):  ref base (0/0):  total (178/252)

Only 100 of 461 total aligned reads displayed.

GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291
                                                                                                   |                                                                                                    
ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                                                                    <  1:1927899/101‑1 (MQ=37)
ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                                                                    <  1:393037/101‑1 (MQ=37)
 gcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                                                                   <  2:2789072/101‑1 (MQ=37)
  cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                  <  1:6146844/101‑1 (MQ=37)
    gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                <  1:755814/101‑1 (MQ=37)
     gaGGCGTGTCAACAGTGGCGCTTTTGCGCAGCCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTGCGGGCAATTCTCCCCCCGAGGTATTTGGAGCAAGATg                                                                                               <  1:105702/100‑1 (MQ=21)
      aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGa                                                                                              >  1:8331764/1‑101 (MQ=25)
        gCGTGTCACCCGTGGGCCTTTTGCGCAGGCAAAAGGGGGGCCGTGCCCCCCTCTTTGC.CTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                            <  1:5079464/101‑1 (MQ=25)
        gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                            >  1:1190776/1‑101 (MQ=25)
         cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCC.CTGGCCCTCGGCCCATTCCCCCCCCCAGGGATTTTGAGCCAGAAGaaaa                                                                                           >  1:5175079/1‑101 (MQ=255)
         cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                           <  1:3181111/101‑2 (MQ=25)
          gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                          <  1:801475/101‑3 (MQ=25)
            gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                        <  2:2069674/101‑5 (MQ=25)
              cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGGCCCCCTC.CTGGCCTTCGGCCCATTCACCCCCCGAGGTATTTGGGGCAAAAAGAAAA.ACCAt                                                                                     >  2:5733147/1‑101 (MQ=255)
               aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                    <  1:2781611/101‑1 (MQ=25)
                aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                 <  2:749244/101‑1 (MQ=25)
                     ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTcc                                                                            >  2:8366810/1‑100 (MQ=21)
                     ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                                            <  2:5540963/101‑1 (MQ=25)
                      gcgcTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCAt                                                                           <  2:5451495/101‑1 (MQ=21)
                       cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATc                                                                          <  1:5297026/101‑1 (MQ=25)
                        gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCt                                                                         <  2:7927121/101‑1 (MQ=25)
                         cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                                        <  2:7678640/101‑1 (MQ=25)
                            ttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGcc                                                                     >  2:2694469/1‑101 (MQ=21)
                             tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCt                                                                    <  2:6734283/101‑1 (MQ=25)
                             ggcgaAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCC.ATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                   <  2:6172181/100‑1 (MQ=21)
                              gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                   <  1:4900924/101‑1 (MQ=25)
                               cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTCTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCa                                                                  <  1:1595539/101‑1 (MQ=25)
                                 cAGGCAAAAGGGGGGGGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCAAGGTATTTGGAGGAAGATGAAGA.ACCTTG..GGTTCATCTTGCCTCaaa                                                                >  2:5297274/1‑101 (MQ=21)
                                   ggCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                              <  1:556535/101‑1 (MQ=25)
                                   ggCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                              <  1:7054335/101‑1 (MQ=21)
                                   cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAatat                                                             >  2:8830258/5‑101 (MQ=17)
                                    gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAatat                                                             <  1:4395948/101‑1 (MQ=25)
                                     cgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                           >  2:4542344/3‑101 (MQ=17)
                                     cgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                           >  2:3793075/3‑101 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                          <  1:3034239/100‑1 (MQ=17)
                                       aaggtggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                          <  2:3567799/96‑1 (MQ=21)
                                       aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                          <  2:3274006/101‑1 (MQ=25)
                                       aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGGTCATCTTGCCTCAAATATccc                                                          >  2:8589697/1‑101 (MQ=21)
                                       aaaGGGGGGCGCTGCCACCCTC.TTGGCCTTCGGACAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                          <  2:6958684/101‑1 (MQ=25)
                                       aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                         >  1:7898947/1‑101 (MQ=17)
                                       aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                         >  1:3685126/1‑101 (MQ=17)
                                        aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                        >  1:1414557/4‑101 (MQ=17)
                                        aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                        <  1:8172126/98‑1 (MQ=17)
                                        aaGGGCGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCg                                                         <  2:8246678/101‑1 (MQ=21)
                                          agggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                      >  2:1322702/2‑101 (MQ=18)
                                          agggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                      >  1:99828/2‑101 (MQ=18)
                                           gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                                      >  1:3343208/1‑101 (MQ=21)
                                            ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCggggg                                                     <  1:4324482/101‑1 (MQ=25)
                                            ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAACTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggt                                                    <  1:2686138/101‑2 (MQ=18)
                                            ggggCGCTG.CCCCCTC.TTGGCCTTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                   <  2:3115691/101‑1 (MQ=2)
                                              ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                  >  1:6834317/1‑98 (MQ=21)
                                              ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  1:910412/101‑1 (MQ=25)
                                               gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  1:3084851/101‑1 (MQ=25)
                                                cgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAgg                                                 >  2:7832847/1‑100 (MQ=25)
                                                cgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                 >  2:3912708/1‑101 (MQ=25)
                                                 gcTGCCCCCCTC.TTGGCCTTCGGGCAATTCACCCCCCGGGGTATTTTGAGCAAGAAG.AAAGAACATG..GGGTCATCTTGCCTCCAATATCCCCGGGGGGGTc                                                >  2:1721476/1‑101 (MQ=12)
                                                 gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  1:359358/1‑95 (MQ=21)
                                                 gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  2:6608536/1‑95 (MQ=21)
                                                 gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                >  2:4100169/1‑101 (MQ=25)
                                                  cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                              >  2:711372/1‑94 (MQ=25)
                                                  cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  2:1909774/101‑1 (MQ=25)
                                                  cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  1:8871747/101‑1 (MQ=25)
                                                  cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  1:6588502/101‑1 (MQ=25)
                                                   tGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  2:6406878/101‑1 (MQ=25)
                                                   tGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  1:647799/101‑1 (MQ=25)
                                                    gCCCCCCTC.TTGGCCTTCTGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                             <  1:6339846/101‑1 (MQ=25)
                                                    gCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                             <  2:7326215/101‑1 (MQ=37)
                                                      cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  2:3224775/1‑101 (MQ=25)
                                                           tc.tTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  1:4157754/101‑1 (MQ=37)
                                                              ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  2:4649357/6‑101 (MQ=37)
                                                              ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    <  2:9152982/96‑1 (MQ=37)
                                                                 gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 >  1:4711634/3‑101 (MQ=37)
                                                                   cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  2:1159893/1‑101 (MQ=37)
                                                                   cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTAATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  1:544283/1‑101 (MQ=37)
                                                                     tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             <  1:5809196/101‑1 (MQ=255)
                                                                     tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             <  2:151925/101‑1 (MQ=255)
                                                                      cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            <  1:2049429/101‑1 (MQ=255)
                                                                      cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:5140365/1‑101 (MQ=255)
                                                                           aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  1:5758641/1‑101 (MQ=37)
                                                                           aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGAACATG..GGTTTATCTTGCCTCAAATATCTCGGGGGGAAACAGGAAAGGAGGGGGGGCACGGccca                       >  2:2931737/1‑100 (MQ=16)
                                                                             ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     >  2:5052234/1‑101 (MQ=255)
                                                                               ccccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   <  1:8590082/99‑1 (MQ=255)
                                                                               caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   <  2:4292131/99‑1 (MQ=255)
                                                                                accccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  1:4615711/2‑101 (MQ=255)
                                                                                  cccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                >  2:2580365/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          <  2:4343866/101‑1 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2470700/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGAACATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          <  1:2883537/101‑1 (MQ=255)
                                                                                         ggTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         >  1:7772028/1‑101 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        >  2:349315/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:8086581/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCGTtcg       >  1:7341864/1‑100 (MQ=25)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      <  2:2338201/101‑1 (MQ=255)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:2447354/1‑101 (MQ=255)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  1:866456/1‑101 (MQ=255)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      <  2:8754053/101‑1 (MQ=255)
                                                                                            aTTGGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGACGCCCCCTCCCGGGTCTTtctc      >  1:7225717/1‑101 (MQ=25)
                                                                                             tttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  1:1282932/101‑1 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:3805831/1‑101 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:4786925/1‑101 (MQ=255)
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GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG