Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,209 A→T 10.6% intergenic (‑185/+91) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2090A→T10.6% 139.3 ‑3.3 ‑0.0476intergenic (‑185/+91)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (31/19):  ref base (193/230):  total (226/250)
Fisher's exact test strand distribution p-value = 3.55e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 505 total aligned reads displayed.

TCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGG  >  NC_014034/2640109‑2640309
                                                                                                       |                                                                                                    
   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:6289534/101‑1 (MQ=21)
   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:8268564/101‑1 (MQ=25)
       tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                               <  2:5471634/101‑1 (MQ=25)
         gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                             <  2:789557/101‑1 (MQ=25)
          cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTT.GGCC.TTCGGCCAATTCACCCCCCCAGGGATTTGGAGCAAGATG.AAAGACCATAGGGTCATc                                                                                            >  2:3262916/1‑101 (MQ=18)
           gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                                           <  2:7145618/101‑1 (MQ=21)
            cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                                          <  1:8549792/101‑1 (MQ=21)
            cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                                          <  1:7383529/101‑1 (MQ=21)
             ttgtGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                         <  2:4559164/98‑1 (MQ=21)
              tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGc                                                                                        <  2:7390367/101‑1 (MQ=25)
               ttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGcc                                                                                       <  2:4710028/101‑1 (MQ=25)
                tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                                      <  2:6734283/101‑1 (MQ=25)
                ggcgaAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCC.ATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                     <  2:6172181/100‑1 (MQ=21)
                  cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                                    <  1:911566/101‑1 (MQ=25)
                    cAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                                  <  2:3178298/101‑1 (MQ=25)
                    cAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                                  >  2:1252188/1‑101 (MQ=21)
                     aGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                 <  2:2580534/101‑1 (MQ=21)
                      cccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                               >  2:8830258/5‑101 (MQ=17)
                       gCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                               >  1:7100347/1‑101 (MQ=21)
                       ccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                              >  1:3090450/4‑101 (MQ=17)
                        cgaaaAGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                             >  1:4307606/3‑101 (MQ=17)
                        cAAAAGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                              <  2:8102212/101‑1 (MQ=21)
                         aaaaGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                             >  1:5204422/1‑101 (MQ=21)
                          aaggtggggCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                            <  2:3567799/96‑1 (MQ=21)
                          aaaGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                            <  1:6121047/101‑1 (MQ=21)
                          aaaGGGGGGCGCTGCCCCCCCCTT.GGCC.TTCGGCCAATTTACCCCCCGAGGCATTTGGAGCAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATccc                                                                            >  1:5773598/1‑101 (MQ=21)
                          aaaAGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                           <  2:5918091/101‑1 (MQ=17)
                          aaaAGGGGGCGCTGCCCCCCTCTT.GG.CTTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                           <  2:524788/101‑1 (MQ=18)
                           aaGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                                           <  2:7566000/101‑1 (MQ=21)
                            aGGGGGGCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgg                                                                          <  2:5403377/101‑1 (MQ=25)
                                gggCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                     <  1:5077480/101‑3 (MQ=18)
                                 ggCGCTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                    >  2:3800358/1‑98 (MQ=18)
                                    gcTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                 >  2:6608536/1‑95 (MQ=21)
                                    gcTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                  >  1:7960366/1‑101 (MQ=25)
                                     cTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                 <  2:1909774/101‑1 (MQ=25)
                                     cTGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                 >  2:647789/1‑101 (MQ=25)
                                      tGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                <  1:647799/101‑1 (MQ=25)
                                      tGCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                >  2:1781353/1‑101 (MQ=25)
                                       gCCCCCCTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                               <  1:3640360/101‑1 (MQ=25)
                                        ccccccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                              <  1:6701647/101‑1 (MQ=37)
                                         cccccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                             >  2:4132451/1‑101 (MQ=37)
                                         cccccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                             <  1:1252220/101‑1 (MQ=37)
                                         cccccACCT.GGCC.TTCGGCCAATTCACCCCACGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                             <  1:2927739/101‑1 (MQ=25)
                                         atctctctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                             <  2:5119532/97‑1 (MQ=25)
                                          ccccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                            >  1:1422510/1‑101 (MQ=37)
                                           cccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                           >  1:281226/1‑101 (MQ=37)
                                            ccTCTTGGG.C.TTTGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                          <  2:8472702/101‑1 (MQ=25)
                                            ccTCTT.GGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                          <  2:1490003/101‑1 (MQ=37)
                                            ccTCTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                          <  2:3066294/101‑1 (MQ=37)
                                              tctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                        >  2:1048737/1‑101 (MQ=25)
                                               ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                       >  2:3409478/1‑101 (MQ=37)
                                               ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                       >  1:3282429/1‑101 (MQ=37)
                                                 ttggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                      >  1:7661104/6‑101 (MQ=37)
                                                  tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                     >  2:6918996/5‑101 (MQ=37)
                                                  tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                     >  1:8167670/5‑101 (MQ=37)
                                                   ggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                    >  2:8706250/4‑101 (MQ=37)
                                                    gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                   >  1:2032158/3‑101 (MQ=37)
                                                    gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                   >  1:4711634/3‑101 (MQ=37)
                                                      c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                 >  2:1159893/1‑101 (MQ=37)
                                                      c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                 <  2:5663938/101‑1 (MQ=255)
                                                      c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTAATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                 >  1:544283/1‑101 (MQ=37)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                               <  2:2350588/101‑1 (MQ=255)
                                                          cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                              >  1:171767/1‑101 (MQ=255)
                                                          cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                              <  1:6923506/101‑1 (MQ=255)
                                                           ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                             <  1:5297458/101‑1 (MQ=255)
                                                             ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                           <  1:6561239/101‑1 (MQ=37)
                                                             ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                           >  1:487965/1‑101 (MQ=255)
                                                              cAATTCACCCCCCGAGGTATTTGGGGAAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCCGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcca                                          >  1:62559/1‑100 (MQ=25)
                                                               aaTTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                         <  1:3877799/101‑1 (MQ=255)
                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                        >  1:1093358/1‑101 (MQ=37)
                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                      >  2:4484743/4‑101 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                     >  1:2242472/3‑101 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                     >  1:5593669/3‑101 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                     <  2:4292131/99‑1 (MQ=255)
                                                                    accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                    >  2:7039601/2‑101 (MQ=255)
                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                   <  1:3037537/101‑1 (MQ=255)
                                                                        cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                >  1:8292430/1‑101 (MQ=255)
                                                                         ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                               <  2:7947076/101‑1 (MQ=255)
                                                                           gAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                             <  2:27347/101‑1 (MQ=255)
                                                                              gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                          >  2:349315/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                        >  2:4996385/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                        >  1:866456/1‑101 (MQ=255)
                                                                                aTTGGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGACGCCCCCTCCCGGGTCTTtctc                        >  1:7225717/1‑101 (MQ=25)
                                                                                     gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                   >  1:3727543/4‑101 (MQ=255)
                                                                                        cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                >  2:2080902/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc               >  1:8865344/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc               <  1:4584204/101‑1 (MQ=255)
                                                                                         aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc               >  1:1341909/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc               >  1:2570351/1‑101 (MQ=255)
                                                                                             atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc            >  2:2485886/4‑101 (MQ=255)
                                                                                             atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc            >  1:3433116/4‑101 (MQ=255)
                                                                                             atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc            >  2:4802669/4‑101 (MQ=255)
                                                                                              tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt           >  2:9116962/3‑101 (MQ=255)
                                                                                                aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa         <  1:4132570/101‑1 (MQ=255)
                                                                                                aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa         >  2:288897/1‑101 (MQ=255)
                                                                                                    aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc     <  1:2761264/101‑1 (MQ=255)
                                                                                                    aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc     >  1:7867656/1‑101 (MQ=255)
                                                                                                     ccATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc    <  2:7793053/101‑1 (MQ=255)
                                                                                                      cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg   <  2:3973544/101‑1 (MQ=255)
                                                                                                       aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg  >  1:4489286/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
TCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGG  >  NC_014034/2640109‑2640309

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG