Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,216 C→T 10.9% intergenic (‑192/+84) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2160C→T10.9% 124.6 ‑1.9 0.0485intergenic (‑192/+84)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (32/21):  ref base (212/218):  total (246/239)
Fisher's exact test strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 514 total aligned reads displayed.

TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316
                                                                                                        |                                                                                                    
 ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTcc                                                                                                     >  2:8366810/1‑100 (MQ=21)
 ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                                                                     <  1:8619762/101‑1 (MQ=25)
   cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTTGGCC.TTCGGCCAATTCACCCCCCCAGGGATTTGGAGCAAGATG.AAAGACCATA..GGGTCATc                                                                                                   >  2:3262916/1‑101 (MQ=18)
     cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                                                                 <  2:7009971/101‑1 (MQ=25)
         tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGGATTTGGAGCCAGATG.AAAGACCAAG..GGTTCATCTTGCCt                                                                                             >  2:2577157/1‑101 (MQ=21)
           cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCa                                                                                           <  2:7916732/101‑1 (MQ=21)
             cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCaaa                                                                                         >  2:1252188/1‑101 (MQ=21)
             cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCaaa                                                                                         <  2:3178298/101‑1 (MQ=25)
              ccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGG..GGTT.ATCTTGCCCCAAata                                                                                       >  1:2123557/6‑101 (MQ=9)
              aGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                                                        <  2:8175278/101‑1 (MQ=25)
              aGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                                                        <  1:6354434/101‑1 (MQ=25)
               ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                                                       <  1:556535/101‑1 (MQ=25)
                 cgaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                                    >  1:4307606/3‑101 (MQ=17)
                  gaaaAGGGGGTGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                                                   >  1:5416497/2‑101 (MQ=18)
                  gaaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                                                   <  1:3034239/100‑1 (MQ=17)
                  aaaaGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATcc                                                                                    <  1:279756/101‑1 (MQ=25)
                  aaaaGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCCTGCCTCAAATATcc                                                                                    <  1:7171778/101‑1 (MQ=21)
                  aaaaGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGGTCATCTTGCCTCCAATATcc                                                                                    >  1:8035015/1‑101 (MQ=18)
                  aaaaGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATcc                                                                                    >  1:5204422/1‑101 (MQ=21)
                   aaaGGGGGGCGCTGCCCCCCCCTTGGCC.TTCGGCCAATTTACCCCCCGAGGCATTTGGAGCAAGATGAAAA.ACCATG..GGTTCATCTTTCCTCAAATATccc                                                                                   >  1:5773598/1‑101 (MQ=21)
                   aaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                                  >  2:3996887/1‑101 (MQ=17)
                   aaaAGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                                  <  2:5918091/101‑1 (MQ=17)
                   aaaAGGGGGCGCTGCCCCCCTCTTGG.CTTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                                  <  2:524788/101‑1 (MQ=18)
                     aGGGGGGCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgg                                                                                 <  2:5403377/101‑1 (MQ=25)
                      agggggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                                               >  1:99828/2‑101 (MQ=18)
                       gggggCGCTG.CCCCCTCTTGGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                                                             <  1:7814630/101‑1 (MQ=2)
                        ggggCGCTG.CCCCCTCTTGGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                                            <  2:3525400/101‑1 (MQ=2)
                         gggCGCTGCCCCCCTCTTGGCC.TTCGGCTAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggggg                                                                             <  2:780099/101‑1 (MQ=25)
                         gggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                                            <  1:5077480/101‑3 (MQ=18)
                         gggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                                            <  2:8851178/101‑3 (MQ=18)
                          ggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                            <  2:4645515/101‑1 (MQ=25)
                          ggCGCTGCCCCCCTCTTGGCC.TTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                            <  1:910412/101‑1 (MQ=25)
                           gcgcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                           <  1:3084851/101‑1 (MQ=25)
                            cgcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                         >  2:803298/1‑96 (MQ=21)
                             gcTGCCCCCCTCTTGGCC.TTCGGGCAATTCACCCCCCGGGGTATTTTGAGCAAGAAG.AAAGAACATG..GGGTCATCTTGCCTCCAATATCCCCGGGGGGGTc                                                                         >  2:1721476/1‑101 (MQ=12)
                             gcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                        >  1:8864799/1‑95 (MQ=21)
                             gcTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                         <  2:453995/101‑1 (MQ=25)
                              cTGCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                        >  2:647789/1‑101 (MQ=25)
                                gCCCCCCTCTTGGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      <  2:7326215/101‑1 (MQ=37)
                                gCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      <  1:3640360/101‑1 (MQ=25)
                                gCCCCCCTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      <  2:550863/101‑1 (MQ=37)
                                  cccccTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                                    <  1:1252220/101‑1 (MQ=37)
                                  atctctctTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                                    <  2:5119532/97‑1 (MQ=25)
                                   ccccTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                                   >  1:1422510/1‑101 (MQ=37)
                                     ccTCTTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                                 <  2:3066294/101‑1 (MQ=37)
                                        ctTGGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                              >  2:3409478/1‑101 (MQ=37)
                                         ttggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                             >  1:7661104/6‑101 (MQ=37)
                                            gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                          >  1:4711634/3‑101 (MQ=37)
                                            gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                          >  1:323433/3‑101 (MQ=37)
                                                  cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                                     >  1:2689729/1‑101 (MQ=255)
                                                   ggCCAATTCACCCCCCGNGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                    <  2:2783888/101‑1 (MQ=37)
                                                   ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                    <  1:5297458/101‑1 (MQ=255)
                                                     ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                                  >  1:487965/1‑101 (MQ=255)
                                                     ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                                  <  1:6536494/101‑1 (MQ=255)
                                                       aaTTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                <  1:3877799/101‑1 (MQ=255)
                                                        aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                               >  1:1093358/1‑101 (MQ=37)
                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                              >  2:9118210/1‑101 (MQ=25)
                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGCCGGGGGGCAGAGCCCCCCt                                             >  2:2299669/4‑101 (MQ=25)
                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                             >  2:2398873/4‑101 (MQ=25)
                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                             <  2:5697428/98‑1 (MQ=255)
                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                            >  1:192223/3‑101 (MQ=255)
                                                                  cGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                                     >  1:6171331/1‑101 (MQ=255)
                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                    <  2:1423796/101‑1 (MQ=255)
                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                   <  2:4343866/101‑1 (MQ=255)
                                                                    aGGTATTTGGAGCAAGATG.AAAGAACATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                   <  1:2883537/101‑1 (MQ=255)
                                                                       tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                >  2:3848884/1‑101 (MQ=255)
                                                                       tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCGTtcg                                >  1:7341864/1‑100 (MQ=25)
                                                                        aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCNCCTCCCGGGTCTTtctc                               >  2:3675953/1‑101 (MQ=255)
                                                                        aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                               >  2:4996385/1‑101 (MQ=255)
                                                                        aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                               >  1:6870534/1‑101 (MQ=255)
                                                                           tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                            >  1:587260/1‑101 (MQ=255)
                                                                           tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                            >  2:2906232/1‑101 (MQ=255)
                                                                             gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                          >  1:3727543/4‑101 (MQ=255)
                                                                               gcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                        <  1:8291119/100‑1 (MQ=255)
                                                                                cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                       >  1:3378181/1‑101 (MQ=255)
                                                                                 aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                      >  1:1341909/1‑101 (MQ=255)
                                                                                 aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                      <  1:4584204/101‑1 (MQ=255)
                                                                                 aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCCCCCGGGCCTTTCTCAAGCGtccg                      >  1:6655238/1‑98 (MQ=25)
                                                                                   agagtaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg                     <  2:4242161/96‑1 (MQ=255)
                                                                                    gatgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg                    >  1:7954662/5‑101 (MQ=255)
                                                                                     atgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc                   >  2:6156111/4‑101 (MQ=255)
                                                                                      tgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt                  >  1:2419308/3‑101 (MQ=255)
                                                                                      tgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTTCTCAAGCGTTCCGGCt                  >  2:7594082/3‑101 (MQ=255)
                                                                                       gaaaGACCATG..GGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTTTTCAAACGTTTCgggcc                 >  2:6806486/2‑98 (MQ=25)
                                                                                        aaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa                <  1:4132570/101‑1 (MQ=255)
                                                                                        aaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa                <  2:6620821/101‑1 (MQ=255)
                                                                                         aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg               <  2:4711485/101‑1 (MQ=255)
                                                                                         aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg               >  1:7061158/1‑101 (MQ=255)
                                                                                         aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCACGGGTCTTTCTCAAGCGTTCCGGCTCAg               >  2:6269522/1‑101 (MQ=255)
                                                                                          aGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa              >  2:190916/1‑101 (MQ=255)
                                                                                            aCCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            >  1:8454206/1‑101 (MQ=255)
                                                                                             ccATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc           <  2:1119169/101‑1 (MQ=255)
                                                                                              cATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg          <  2:7111947/101‑1 (MQ=255)
                                                                                               aTG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg         >  1:4489286/1‑101 (MQ=255)
                                                                                                 g..ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGaa       >  1:169242/1‑101 (MQ=255)
                                                                                                     gTTCATCTTGCCTCAAANATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGc     <  2:2043319/101‑1 (MQ=255)
                                                                                                       tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   >  2:7488651/1‑101 (MQ=255)
                                                                                                       tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   <  2:2689647/101‑1 (MQ=255)
                                                                                                        cATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcg  <  2:487957/101‑1 (MQ=255)
                                                                                                        cATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcg  >  2:8736664/1‑101 (MQ=255)
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TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG