Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,728,522 T→G 100% R635R (CGA→CGC RCAP_rcc02539 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,728,5220T→G100.0% 1375.8 400R635R (CGA→CGCRCAP_rcc02539diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (152/247):  ref base (0/0):  total (152/248)

Only 100 of 447 total aligned reads displayed.

CGGGATTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCTCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATCGCGCGCT  >  NC_014034/2728424‑2728619
                                                                                                  |                                                                                                 
  ggATTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCggg                                                                                               >  2:7530498/1‑100 (MQ=255)
   gATTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGGCGCgggg                                                                                              >  2:1002204/1‑99 (MQ=255)
   gATTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGGCGCgggg                                                                                              >  2:499567/1‑99 (MQ=255)
    aTTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGc                                                                                             <  2:4800177/101‑1 (MQ=255)
     tttGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCa                                                                                            <  2:1195138/101‑1 (MQ=255)
      ttGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAg                                                                                           <  2:5141149/101‑1 (MQ=255)
      ttGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAg                                                                                           <  2:6557236/101‑1 (MQ=255)
       tGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGGGCAGa                                                                                          >  1:4913153/1‑101 (MQ=255)
          cGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGaaaa                                                                                       <  1:3991404/101‑1 (MQ=255)
           gtcGATCCGAGGCGAACGGCAAGGCGCGAGACCGCCACACCCGATTTCCGGTCCTCTCCCCCGCCCTCAACCGCTGCCGGGGGCGTTTCGGGTCATAAAAc                                                                                      >  2:2690778/3‑101 (MQ=255)
           gCCGATCCGACGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAc                                                                                      <  2:8802194/101‑1 (MQ=255)
            ccGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAcg                                                                                     <  1:5231198/101‑1 (MQ=255)
            ccGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAcg                                                                                     <  2:6392473/101‑1 (MQ=255)
             cGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAcgc                                                                                    >  2:2369373/1‑101 (MQ=255)
             cGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAcgc                                                                                    <  2:4515213/101‑1 (MQ=255)
             cGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAAcgc                                                                                    <  2:6809632/101‑1 (MQ=255)
               aTCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGGGGGGCAGAAAACgcgc                                                                                  >  1:5665287/1‑101 (MQ=255)
                tCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCa                                                                                 <  1:2012665/101‑1 (MQ=255)
                    aGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAAt                                                                             <  1:8157909/101‑1 (MQ=255)
                      gCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATcc                                                                           <  1:2442828/101‑1 (MQ=255)
                       cGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCcg                                                                          <  1:1117036/101‑1 (MQ=255)
                       cGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCcg                                                                          <  2:3672181/101‑1 (MQ=255)
                       cGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCcg                                                                          <  1:5316892/101‑1 (MQ=255)
                         aaCGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGCGCAGAAAACGCGCAAAATCcgcg                                                                        >  2:8114363/1‑101 (MQ=255)
                         aaCGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCcgcg                                                                        >  1:7308555/1‑101 (MQ=255)
                              cAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcacc                                                                   >  2:7430175/1‑99 (MQ=255)
                               aaGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcatcc                                                                  <  1:7066913/101‑1 (MQ=255)
                                aGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcatcca                                                                 <  1:4116456/101‑1 (MQ=255)
                                 ggCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcatccat                                                                <  1:6980695/101‑1 (MQ=255)
                                  gcgcGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGGGCAGAAAACGCGCAAAATCCGGGGcatccatc                                                               >  1:5726420/1‑101 (MQ=255)
                                  gcgcGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcatccatc                                                               <  1:2819384/101‑1 (MQ=255)
                                  gcacgAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGcatccatc                                                               <  2:490188/98‑1 (MQ=255)
                                     cgAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCggg                                                            >  1:3272318/1‑101 (MQ=255)
                                       aaaGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGGGCAGAAAACGCGCAAAATCCGGGGCATCCATCggggt                                                          >  1:1556358/1‑99 (MQ=255)
                                        aaGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTc                                                         <  1:315184/101‑1 (MQ=255)
                                         aGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGGGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCCCg                                                        >  1:4261006/1‑101 (MQ=255)
                                          gcgcCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGa                                                       <  2:6225255/101‑1 (MQ=255)
                                            gcCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGAcc                                                     >  2:8381325/1‑101 (MQ=255)
                                             ccACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACca                                                    <  2:1087952/101‑1 (MQ=255)
                                                 aGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGa                                                >  2:9143876/1‑101 (MQ=255)
                                                 aGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGa                                                <  1:8506262/101‑1 (MQ=255)
                                                  gTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGAt                                               <  2:5665080/101‑1 (MQ=255)
                                                  gTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGAt                                               <  2:1893241/101‑1 (MQ=255)
                                                  gTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGAt                                               <  2:209265/101‑1 (MQ=255)
                                                  gTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGAt                                               <  1:1002222/101‑1 (MQ=255)
                                                  gTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGAt                                               <  1:6043768/101‑1 (MQ=255)
                                                    cGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAg                                             >  2:7210991/1‑101 (MQ=255)
                                                    cGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAg                                             <  2:5758234/101‑1 (MQ=255)
                                                        ttCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCt                                         <  2:7024653/101‑1 (MQ=255)
                                                        ttCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCt                                         <  1:4715700/101‑1 (MQ=255)
                                                        ttCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCt                                         >  1:5909151/1‑101 (MQ=255)
                                                        ttCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCt                                         <  2:2164280/101‑1 (MQ=255)
                                                         tCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTg                                        >  1:5012878/1‑101 (MQ=255)
                                                          ccGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGc                                       <  2:6009669/101‑1 (MQ=255)
                                                           cGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGcc                                      <  2:6691328/101‑1 (MQ=255)
                                                           cGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGcc                                      >  2:3374582/1‑101 (MQ=255)
                                                            ggTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGccc                                     <  2:8558105/101‑1 (MQ=255)
                                                             gTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCg                                    >  2:1337816/1‑101 (MQ=255)
                                                             gTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCg                                    <  1:3818732/101‑1 (MQ=255)
                                                             gTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCg                                    <  1:8748957/101‑1 (MQ=255)
                                                             gTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCg                                    <  1:6349597/101‑1 (MQ=255)
                                                             gTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCCCGACCACAGATAGCCCTGCCCg                                    >  1:4401127/1‑101 (MQ=255)
                                                               ccTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCCCAGATAGCCCTGCCCgcc                                  >  1:8998241/1‑99 (MQ=255)
                                                                cTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACa                                 >  1:2573342/1‑101 (MQ=255)
                                                                  gTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATc                               <  1:8662848/101‑1 (MQ=255)
                                                                  gTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATc                               <  2:7792683/101‑1 (MQ=255)
                                                                   tCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCg                              <  2:3958303/101‑1 (MQ=255)
                                                                   tCCCCCGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCg                              <  2:8188698/101‑1 (MQ=255)
                                                                    cccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGa                             <  1:5489665/101‑1 (MQ=255)
                                                                     ccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGAc                            <  1:6822814/101‑1 (MQ=255)
                                                                     ccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGAc                            <  2:7970326/101‑1 (MQ=255)
                                                                     ccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGAc                            >  2:3822602/1‑101 (MQ=255)
                                                                     ccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGAc                            >  2:7673259/1‑101 (MQ=255)
                                                                     ccccGCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGAc                            <  1:22735/101‑1 (MQ=255)
                                                                         gCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGccc                        <  2:802950/101‑1 (MQ=255)
                                                                         gCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGccc                        >  2:2503210/1‑101 (MQ=255)
                                                                         gCCCTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGccc                        >  2:2797968/1‑101 (MQ=255)
                                                                          cccTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCa                       <  2:1539400/101‑1 (MQ=255)
                                                                           ccTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAg                      <  2:2998975/101‑1 (MQ=255)
                                                                           ccTCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAg                      <  1:3050833/101‑1 (MQ=255)
                                                                             tCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGGATGCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGAt                    >  1:7506098/1‑101 (MQ=255)
                                                                             tCAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGAt                    <  2:4153184/101‑1 (MQ=255)
                                                                              cAACCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATc                   <  1:1414443/101‑1 (MQ=255)
                                                                               aaCCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCg                  <  1:7180865/101‑1 (MQ=255)
                                                                                aCCGCTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGa                 <  1:3719152/101‑1 (MQ=255)
                                                                                    cTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAg             <  1:7995880/101‑1 (MQ=255)
                                                                                    cTGCGGCGGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAg             <  1:6471990/101‑1 (MQ=255)
                                                                                      gcggcgGGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCAGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCa           >  1:6765588/1‑101 (MQ=255)
                                                                                       cggcggGCGTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCAt          >  1:7680464/1‑101 (MQ=255)
                                                                                        ggcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATc         >  1:889764/1‑101 (MQ=255)
                                                                                        ggcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATc         <  2:1923666/101‑1 (MQ=255)
                                                                                        ggcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATc         <  2:4411400/101‑1 (MQ=255)
                                                                                        ggcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCAAAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATc         >  2:3210955/1‑101 (MQ=255)
                                                                                         gcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATcg        <  1:6206423/101‑1 (MQ=255)
                                                                                         gcgggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATcg        <  2:5482175/101‑1 (MQ=255)
                                                                                           gggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATcgcg      >  1:7934834/1‑101 (MQ=255)
                                                                                           gggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCACTGCCCGACATCGACGCCCAGCTCGAGCAGCATcccg      >  1:1865733/1‑101 (MQ=255)
                                                                                            ggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATcgcgc     >  1:1311164/1‑101 (MQ=255)
                                                                                            ggcgTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATcgcgc     >  1:3559794/1‑101 (MQ=255)
                                                                                               gTCGCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATCGCGCGCt  >  1:1761069/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGGGATTTGACGCCGATCCGAGGCGAACGGCAAGGCGCGAAAGCGCCACAGTCGATTTCCGGTCCTGTCCCCCGCCCTCAACCGCTGCGGCGGGCGTCTCGGAGCAGAAAACGCGCAAAATCCGCGGCATCCATCGGGCTCGACCACAGATAGCCCTGCCCGACATCGACGCCCAGATCGACCAGCATCGCGCGCT  >  NC_014034/2728424‑2728619

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG