Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,770,015 C→G 100% H311Q (CAC→CAG RCAP_rcc02581 → sensor histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,770,0150C→G100.0% 1383.0 371H311Q (CAC→CAGRCAP_rcc02581sensor histidine kinase
Reads supporting (aligned to +/- strand):  new base (220/151):  ref base (0/0):  total (220/151)

Only 100 of 379 total aligned reads displayed.

GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112
                                                                                                   |                                                                                                 
gagaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGt                                                                                                 <  2:8171070/101‑1 (MQ=255)
  gaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTgg                                                                                               <  1:1836811/101‑1 (MQ=255)
    tGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTc                                                                                             >  1:4312335/1‑101 (MQ=255)
    tGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTc                                                                                             <  2:7199831/101‑1 (MQ=255)
    cttccgatctgatCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTc                                                                                             <  1:1649391/92‑1 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            <  1:5446648/101‑1 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            <  2:2521954/101‑1 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            <  2:1120177/101‑1 (MQ=255)
      ggCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGa                                                                                           <  2:4935506/101‑1 (MQ=255)
       gCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGaa                                                                                          >  2:5380313/1‑101 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAg                                                                                         >  1:3014585/1‑101 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGAGGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGagg                                                                                         >  1:4998385/1‑99 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        <  2:6330952/101‑1 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        >  2:8748636/1‑101 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        >  2:4512065/1‑101 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  2:5509608/101‑1 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  1:994958/101‑1 (MQ=255)
             gcccacgccgcTTCCTGAATTGCCGGCGGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  2:6788551/91‑1 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  <  2:2292256/101‑1 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  1:285494/101‑1 (MQ=255)
                 cGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgc                                                                                >  2:3758316/1‑101 (MQ=255)
                 cGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgc                                                                                <  1:8911915/101‑1 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               <  1:7689096/101‑1 (MQ=255)
                   aCGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgc                                                                              <  2:7980577/101‑1 (MQ=255)
                    cGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgcg                                                                             <  1:5698979/101‑1 (MQ=255)
                        ttCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCgg                                                                         >  1:5562109/1‑101 (MQ=255)
                        ttCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCgg                                                                         >  2:7031764/1‑101 (MQ=255)
                        ttCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCgg                                                                         >  1:3346067/1‑101 (MQ=255)
                         tCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCggg                                                                        >  1:7500516/1‑101 (MQ=255)
                         tCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCggg                                                                        >  2:6715306/1‑101 (MQ=255)
                         tCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCggg                                                                        >  1:6728215/1‑101 (MQ=255)
                          ccTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGc                                                                       <  1:2030291/101‑1 (MQ=255)
                          ccTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGc                                                                       >  1:424416/1‑101 (MQ=255)
                          ccTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGc                                                                       >  2:6875145/1‑101 (MQ=255)
                           ggcccatccgcgc.gcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCa                                                                      >  2:2042532/9‑101 (MQ=255)
                           cTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGAGCGCGCCGGGCa                                                                      >  1:9122045/1‑101 (MQ=255)
                            tGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCTCCGGGCAg                                                                     >  1:3884022/1‑101 (MQ=255)
                            tGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAg                                                                     <  1:3471437/101‑1 (MQ=255)
                             gAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGc                                                                    >  2:7577045/1‑101 (MQ=255)
                              aaTGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGcc                                                                   >  1:5065230/1‑101 (MQ=255)
                                tGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCgg                                                                 >  1:3649937/1‑101 (MQ=255)
                                 gTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGt                                                                >  2:3414650/1‑101 (MQ=255)
                                       c.gcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCt                                                          <  2:6292736/101‑1 (MQ=255)
                                           gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       >  1:3555147/1‑101 (MQ=255)
                                           gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       >  1:4365252/1‑101 (MQ=255)
                                           gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggt                                                       <  1:1306742/101‑1 (MQ=255)
                                            aaGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggtg                                                      >  1:1476608/1‑101 (MQ=255)
                                             agagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggtgg                                                     >  2:390574/1‑101 (MQ=255)
                                              gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    <  2:3276855/101‑1 (MQ=255)
                                                  cGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGc                                                >  2:4981558/1‑101 (MQ=255)
                                                   gATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  1:5905770/1‑101 (MQ=255)
                                                   gATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  1:4751017/1‑101 (MQ=255)
                                                    aTGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGccc                                              >  1:4031750/1‑101 (MQ=255)
                                                     tGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcccc                                             <  2:6986105/101‑1 (MQ=255)
                                                      ggcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCg                                            >  1:8516510/1‑101 (MQ=255)
                                                       gcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGa                                           <  1:5225192/101‑1 (MQ=255)
                                                       gcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGa                                           >  1:4510567/1‑101 (MQ=255)
                                                        ggggcgAGCCGGAACGGGTGGAGATCTTCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:7341624/100‑1 (MQ=255)
                                                        cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:4312211/101‑1 (MQ=255)
                                                        cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          >  1:3239881/1‑101 (MQ=255)
                                                         gggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATg                                         <  1:8359489/101‑1 (MQ=255)
                                                         gggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATg                                         >  2:2159221/1‑101 (MQ=255)
                                                           gcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcg                                       >  1:8873184/1‑101 (MQ=255)
                                                            cgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcgc                                      >  2:2845674/1‑101 (MQ=255)
                                                              aGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCg                                    >  2:3675211/1‑101 (MQ=255)
                                                              aGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCg                                    >  2:8971272/1‑101 (MQ=255)
                                                              aGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCg                                    >  1:8536120/1‑101 (MQ=255)
                                                                 ccGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTg                                 >  2:3577277/1‑101 (MQ=255)
                                                                  cGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                <  1:410472/101‑1 (MQ=255)
                                                                   gAACGGGTGGAGCTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCAGGCGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               >  1:8121018/1‑101 (MQ=255)
                                                                   gAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               >  2:8510319/1‑101 (MQ=255)
                                                                    aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  1:2370515/1‑101 (MQ=255)
                                                                    aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  2:4696726/1‑101 (MQ=255)
                                                                     aCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCATGCCCCGATGCGCCGGTGCCgg                             >  2:1884782/1‑101 (MQ=255)
                                                                      cGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGc                            >  1:65919/1‑101 (MQ=255)
                                                                         gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGt                         >  1:1116295/1‑101 (MQ=255)
                                                                         gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGt                         <  1:779628/101‑1 (MQ=255)
                                                                           ggACATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       <  2:2916616/101‑1 (MQ=255)
                                                                             agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     >  1:1002835/1‑101 (MQ=255)
                                                                             agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     >  1:4641261/1‑101 (MQ=255)
                                                                              gaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctgc                    <  2:1634206/101‑1 (MQ=255)
                                                                              gaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctgc                    <  2:7884185/101‑1 (MQ=255)
                                                                                tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcgc                  >  1:7258266/1‑101 (MQ=255)
                                                                                 cTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGcc                 <  1:6162067/101‑1 (MQ=255)
                                                                                 cTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGcc                 >  2:5648125/1‑101 (MQ=255)
                                                                                  tGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGccc                >  1:168717/1‑101 (MQ=255)
                                                                                    cgcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCg              <  1:688499/101‑1 (MQ=255)
                                                                                     gcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGa             >  2:5829460/1‑101 (MQ=255)
                                                                                      ccTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAt            <  2:889180/101‑1 (MQ=255)
                                                                                       cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           <  2:8588698/101‑1 (MQ=255)
                                                                                          tGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgc        <  2:3134539/101‑1 (MQ=255)
                                                                                           gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       <  1:649906/101‑1 (MQ=255)
                                                                                           gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       >  1:2646778/1‑101 (MQ=255)
                                                                                            tCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctg      <  1:8715105/101‑1 (MQ=255)
                                                                                            tCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctg      <  1:3744134/101‑1 (MQ=255)
                                                                                             cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     >  1:6961082/1‑101 (MQ=255)
                                                                                             cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     <  1:7099110/101‑1 (MQ=255)
                                                                                               cgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcgc   >  2:4468837/1‑101 (MQ=255)
                                                                                                gcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGcc  >  2:6518249/1‑101 (MQ=255)
                                                                                                gcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGcc  >  2:6170118/1‑101 (MQ=255)
                                                                                                   |                                                                                                 
GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGC.GCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG