Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,891,981 T→G 100% S460A (TCG→GCG)  thiP → thiamine ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,891,9810T→G100.0% 1480.8 397S460A (TCG→GCG) thiPthiamine ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (204/191):  ref base (0/1):  total (204/193)

Only 100 of 407 total aligned reads displayed.

CCCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891883‑2892078
                                                                                                  |                                                                                                 
  cGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCt                                                                                               >  2:5468098/1‑101 (MQ=255)
   ggCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTg                                                                                              <  1:3467101/101‑1 (MQ=255)
    gCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGt                                                                                             >  1:2911215/1‑101 (MQ=255)
     gtGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                            <  1:947605/100‑1 (MQ=255)
     cTGCGGTTCCTCCCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                            <  2:7967529/101‑1 (MQ=255)
       gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          <  2:7590957/101‑1 (MQ=255)
       gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          >  2:2583216/1‑101 (MQ=255)
       gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          <  2:165837/101‑1 (MQ=255)
        cGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTc                                                                                         >  2:2165617/1‑101 (MQ=255)
        cGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTc                                                                                         >  2:7107532/1‑101 (MQ=255)
         ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        <  2:400011/101‑1 (MQ=255)
         ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        >  1:4077607/1‑101 (MQ=255)
          gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  1:7564176/101‑1 (MQ=255)
          gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  1:6776280/101‑1 (MQ=255)
            tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  1:4557069/1‑101 (MQ=255)
             ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                    >  1:2324826/1‑101 (MQ=255)
             ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTCCCGCGGGGATTGCCGCGGCCTTTTAGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                    >  1:6567948/1‑101 (MQ=255)
               tCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGc                                                                                  >  1:8486791/1‑101 (MQ=255)
                cACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGcc                                                                                 <  1:33399/101‑1 (MQ=255)
                 aCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                >  2:8193566/1‑101 (MQ=255)
                   ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                              <  1:9126509/101‑1 (MQ=255)
                     tGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGc                                                                            <  1:2645673/101‑1 (MQ=255)
                      gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           <  2:3365494/101‑1 (MQ=255)
                      gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           <  1:8272044/101‑1 (MQ=255)
                       ccccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCa                                                                          <  1:86097/101‑1 (MQ=255)
                        cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         <  1:2018753/101‑1 (MQ=255)
                         ccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGa                                                                        <  1:4597729/101‑1 (MQ=255)
                          cGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAc                                                                       >  2:6031316/1‑101 (MQ=255)
                            gCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAccc                                                                     >  1:175738/1‑101 (MQ=255)
                             cTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCt                                                                    <  2:6680425/101‑1 (MQ=255)
                             cTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCt                                                                    <  1:102062/101‑1 (MQ=255)
                              tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   <  2:1566468/101‑1 (MQ=255)
                              tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   >  1:7348367/1‑101 (MQ=255)
                              tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   >  2:2481677/1‑101 (MQ=255)
                               ggCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGc                                                                  <  1:8151735/101‑1 (MQ=255)
                                 cccGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCg                                                                <  1:4003509/101‑1 (MQ=255)
                                   cGGCGGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCt                                                              <  2:8949064/101‑1 (MQ=255)
                                     gCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTg                                                            >  2:7679301/1‑101 (MQ=255)
                                     gCCGACGGGCTTTGCCGCGGGGAATGCCGCGGCCTTTTCGAGGGGCGATCTGGGCGCCGTCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTg                                                            <  2:1079010/101‑1 (MQ=255)
                                       cGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGcc                                                          <  2:2003117/101‑1 (MQ=255)
                                        gACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCg                                                         >  2:4408589/1‑101 (MQ=255)
                                        gACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCg                                                         >  1:4368773/1‑101 (MQ=255)
                                            ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  2:8116704/101‑1 (MQ=255)
                                            ggCTTGGCCGCGGGGGTTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     >  1:7255684/1‑101 (MQ=255)
                                             gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    <  2:1417068/101‑1 (MQ=255)
                                             gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    <  2:8866657/101‑1 (MQ=255)
                                              cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGTCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   <  2:4032106/101‑1 (MQ=255)
                                              cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   >  2:226616/1‑101 (MQ=255)
                                              cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   >  2:7185230/1‑101 (MQ=255)
                                              cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   <  2:3635104/101‑1 (MQ=255)
                                               tttGCTGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  <  1:2438979/101‑1 (MQ=255)
                                               tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  <  1:6969516/101‑1 (MQ=255)
                                                ttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAg                                                 <  2:8540404/101‑1 (MQ=255)
                                                  gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  1:1434948/1‑101 (MQ=255)
                                                    cccgGGGATTGCCCGGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGa                                             <  2:7348043/99‑1 (MQ=255)
                                                     gcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGAt                                            <  2:910852/101‑1 (MQ=255)
                                                      cgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATg                                           <  2:6482888/101‑1 (MQ=255)
                                                      cgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATg                                           <  1:5635752/101‑1 (MQ=255)
                                                       ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                          >  1:4729529/1‑101 (MQ=255)
                                                        gggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCCGACCCTGCCGCTTGCCGTCTATCAGCTGAAggg                                         >  1:8997408/1‑101 (MQ=255)
                                                        gggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATggg                                         <  2:2743383/101‑1 (MQ=255)
                                                           aTTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAg                                      >  1:3066315/1‑101 (MQ=255)
                                                           aTTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAg                                      <  2:5050986/101‑1 (MQ=255)
                                                            ttGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGc                                     <  1:5573337/101‑1 (MQ=255)
                                                             tGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCt                                    >  2:9028356/1‑101 (MQ=255)
                                                              gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   <  1:5450180/101‑1 (MQ=255)
                                                                     ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:5291477/101‑1 (MQ=255)
                                                                     ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:684456/101‑1 (MQ=255)
                                                                     ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGCCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:5765439/101‑1 (MQ=255)
                                                                      cTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATGGCATgg                           >  2:2917675/1‑101 (MQ=255)
                                                                      cTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATgg                           >  1:6658956/1‑101 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         >  1:3735069/1‑101 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         <  2:4830686/101‑1 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         <  1:4293329/101‑1 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         >  1:1204498/1‑101 (MQ=255)
                                                                         ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                        >  1:636953/1‑101 (MQ=255)
                                                                         ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                        <  2:5178915/101‑1 (MQ=255)
                                                                          tCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCa                       >  1:5020115/1‑101 (MQ=255)
                                                                          tCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCACAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCa                       >  1:7098347/1‑101 (MQ=255)
                                                                           cGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTGTCAGCTGATGGGCAGCTATCGCATGGATCAg                      >  1:5868993/1‑101 (MQ=255)
                                                                           cGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAg                      >  1:4843652/1‑101 (MQ=255)
                                                                            gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                     <  2:4229992/101‑1 (MQ=255)
                                                                            gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                     >  2:182613/1‑101 (MQ=255)
                                                                              tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   <  2:6208232/101‑1 (MQ=255)
                                                                              tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   >  2:8441459/1‑101 (MQ=255)
                                                                              tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   >  1:8205418/1‑101 (MQ=255)
                                                                               gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                  >  1:3206012/1‑101 (MQ=255)
                                                                                   gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg              <  2:5663533/101‑1 (MQ=255)
                                                                                    aTCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgc             >  1:2535476/1‑101 (MQ=255)
                                                                                      cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc           >  1:7739634/1‑101 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  2:3120011/1‑101 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  2:6951928/1‑101 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  2:463384/1‑101 (MQ=255)
                                                                                        gggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg         <  1:3514594/101‑1 (MQ=255)
                                                                                        gggCGCCATCCCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg         <  1:8115646/101‑1 (MQ=255)
                                                                                         ggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGc        >  1:7903888/1‑101 (MQ=255)
                                                                                           cgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCtt      <  1:5287907/101‑1 (MQ=255)
                                                                                            gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCttc     >  2:1329416/1‑100 (MQ=255)
                                                                                            gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTg     <  1:5737711/101‑1 (MQ=255)
                                                                                             ccATCGCGCTGTTTTCCGATGGCCGCAGCCAGCCCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGc    <  1:6029629/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891883‑2892078

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG