Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,925,566 A→C 100% intergenic (+350/‑85) RCAP_rcc02735 → / → RCAP_rcc02736 virulence‑associated protein E/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,925,5660A→C100.0% 2353.0 629intergenic (+350/‑85)RCAP_rcc02735/RCAP_rcc02736virulence‑associated protein E/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (319/309):  ref base (0/0):  total (320/309)

Only 100 of 642 total aligned reads displayed.

AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663
                                                                                                  |                                                                                                 
aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                                                                 >  1:589039/1‑101 (MQ=255)
 ggTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCa                                                                                                <  1:7907621/101‑1 (MQ=255)
  gTGGGACAAGCATTGTTCTGCAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAt                                                                                               <  1:5645359/101‑1 (MQ=255)
  gTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAt                                                                                               >  1:5737952/1‑101 (MQ=255)
   tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              >  1:6366820/1‑101 (MQ=255)
   tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              <  1:4497684/101‑1 (MQ=255)
   tGGGACAAGCATTGTTCCGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              >  2:5639691/1‑101 (MQ=255)
    gggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAttt                                                                                             >  2:3191817/1‑101 (MQ=255)
     ggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtttt                                                                                            >  1:3122113/1‑101 (MQ=255)
     ggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtttt                                                                                            >  1:8759188/1‑101 (MQ=255)
      gACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTc                                                                                           <  2:8095689/101‑1 (MQ=255)
      gACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTc                                                                                           >  2:4460564/1‑101 (MQ=255)
        cAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCt                                                                                         >  2:8833782/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        >  2:8344464/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        <  2:4020484/101‑1 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        >  2:6007820/1‑101 (MQ=255)
          aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTg                                                                                       <  1:964381/101‑1 (MQ=255)
          aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTg                                                                                       >  1:1117091/1‑101 (MQ=255)
           gCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGa                                                                                      <  1:754586/101‑1 (MQ=255)
             aTTGTTCTGAAAGGAAAATTCGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTCATc                                                                                    <  1:7229357/101‑1 (MQ=255)
              ttGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCg                                                                                   <  1:8653782/101‑1 (MQ=255)
                gTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAg                                                                                 <  1:5203128/101‑1 (MQ=255)
                gTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAg                                                                                 >  2:7437838/1‑101 (MQ=255)
                gTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAg                                                                                 >  1:7320233/1‑101 (MQ=255)
                 ttCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAgg                                                                                >  2:6560319/1‑100 (MQ=255)
                   cTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGc                                                                              >  2:1716428/1‑101 (MQ=255)
                   cTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGc                                                                              >  1:1383957/1‑101 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            >  1:4700166/1‑101 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  1:6542464/101‑1 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGCATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          >  1:361445/1‑101 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         >  2:249969/1‑101 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCaa                                                                        <  1:1899332/101‑1 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCaa                                                                        >  1:3860613/1‑101 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCaa                                                                        >  2:7860795/1‑101 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCACATTTTCCTTGATCGAGTGCCTGCaa                                                                        <  2:6267365/101‑1 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  1:7664673/1‑101 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    <  2:2042389/101‑1 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 >  1:8540244/1‑101 (MQ=255)
                                 tGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTc                                                                <  2:2371318/101‑1 (MQ=255)
                                 tGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTc                                                                <  1:8053093/101‑1 (MQ=255)
                                        ccACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTc                                                         <  2:6718657/101‑1 (MQ=255)
                                         cACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCa                                                        >  2:7563657/1‑101 (MQ=255)
                                         cACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCa                                                        <  1:4987310/101‑1 (MQ=255)
                                         cACGTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCa                                                        <  1:4350917/101‑1 (MQ=255)
                                          aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       >  1:1015318/1‑101 (MQ=255)
                                          aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       >  2:8588610/1‑101 (MQ=255)
                                           cTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      >  1:1792301/1‑101 (MQ=255)
                                           cTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      >  2:6398224/1‑101 (MQ=255)
                                           cTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      <  1:5450047/101‑1 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCACCGTGATTCCCTGCTCATGa                                                     >  1:2971984/1‑101 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     <  2:9001789/101‑1 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     >  1:8971222/1‑101 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     >  1:2705066/1‑101 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGGCGGGGGCATTTTCCTTGATCGAGAGCCTGCAACGTGATTCCCTGCTCATGa                                                     >  1:9119142/1‑101 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAc                                                    >  2:5367353/1‑101 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAc                                                    >  1:6161226/1‑101 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAc                                                    >  2:3914213/1‑101 (MQ=255)
                                              gAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAcc                                                   <  1:70613/101‑1 (MQ=255)
                                                 cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                >  1:8406210/1‑101 (MQ=255)
                                                   gtgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGc                                              <  2:2138816/95‑1 (MQ=255)
                                                   gtgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGc                                              <  1:2810823/95‑1 (MQ=255)
                                                    tgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcc                                             >  1:6420808/6‑101 (MQ=255)
                                                    gggagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcc                                             <  1:8881815/96‑1 (MQ=255)
                                                     gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            >  2:6147958/5‑101 (MQ=255)
                                                      aagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTNCTCATGACCAAAGCCGa                                           >  2:3396672/4‑101 (MQ=255)
                                                       agtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGaa                                          >  2:6949997/3‑101 (MQ=255)
                                                        gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTTGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         >  2:4342744/2‑101 (MQ=255)
                                                          gggACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGc                                       >  2:1325731/1‑101 (MQ=255)
                                                             aCAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  2:1383925/101‑1 (MQ=255)
                                                              cAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCAc                                   <  2:3798727/101‑1 (MQ=255)
                                                              cAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCAc                                   <  2:1897573/101‑1 (MQ=255)
                                                               aGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACg                                  >  2:1223295/1‑101 (MQ=255)
                                                                 cccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGCTCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGAt                                <  2:5229002/101‑1 (MQ=255)
                                                                   cAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATcc                              >  2:3522523/1‑101 (MQ=255)
                                                                    aaaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCa                             >  2:7104105/1‑101 (MQ=255)
                                                                     aaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAg                            >  2:3696420/1‑101 (MQ=255)
                                                                      aGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAgg                           <  2:2816137/101‑1 (MQ=255)
                                                                        cGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTg                         >  2:7728715/1‑101 (MQ=255)
                                                                         gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        <  1:206751/101‑1 (MQ=255)
                                                                         gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        <  1:1556112/101‑1 (MQ=255)
                                                                              gggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCtt                   >  2:1587721/1‑101 (MQ=255)
                                                                              gggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCtt                   >  1:8262979/1‑101 (MQ=255)
                                                                                gACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGACACTtca                 >  2:7277782/1‑101 (MQ=255)
                                                                                 aCATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcat                <  1:3733788/101‑1 (MQ=255)
                                                                                 aCATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcat                <  1:6057940/101‑1 (MQ=255)
                                                                                  cATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatc               >  2:847385/1‑101 (MQ=255)
                                                                                  cATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatc               <  1:4080183/101‑1 (MQ=255)
                                                                                   aTGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatca              >  1:3412285/1‑101 (MQ=255)
                                                                                    tGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTcatcat             >  2:4808895/1‑101 (MQ=255)
                                                                                     ggATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATg            >  1:6811956/1‑101 (MQ=255)
                                                                                         gCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAccc        <  2:4588644/101‑1 (MQ=255)
                                                                                         gCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAccc        <  2:4159354/101‑1 (MQ=255)
                                                                                           ggTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCCCGATCCCGGGCAGCCTTCAGCACGACCCCg      >  2:2970782/1‑101 (MQ=255)
                                                                                            gTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGa     <  1:2289878/101‑1 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   <  2:5079551/101‑1 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   >  2:5739878/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  1:4820819/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  <  2:4930358/101‑1 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  1:6864072/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  2:2639312/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG