Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,715 G→C 7.7% intergenic (+58/‑102) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7150G→C 7.7% 89.8 ‑1.4 ‑1.9519intergenic (+58/‑102)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (23/17):  ref base (225/254):  total (248/271)
Fisher's exact test strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.27e-02

Only 100 of 540 total aligned reads displayed.

CGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAC  >  NC_014034/2950615‑2950815
                                                                                                    |                                                                                                      
 ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGc                                                                                                       >  1:7906151/1‑101 (MQ=255)
  gggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGcc                                                                                                      <  2:4645752/101‑1 (MQ=255)
   ggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGccc                                                                                                     <  2:2647049/101‑1 (MQ=255)
     tcccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCt                                                                                                   <  2:861175/99‑1 (MQ=255)
     tGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCt                                                                                                   <  2:7959097/101‑1 (MQ=255)
      gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                                  <  1:8708565/101‑1 (MQ=255)
      gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                                  >  2:7683523/1‑101 (MQ=255)
       ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCttt                                                                                                 <  2:664066/101‑1 (MQ=255)
       ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCttt                                                                                                 <  2:2129933/101‑1 (MQ=255)
        cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTc                                                                                                <  1:1872227/101‑1 (MQ=255)
        cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTc                                                                                                <  2:3810045/101‑1 (MQ=255)
         gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                               >  2:3275403/1‑101 (MQ=255)
         gccCTCGCCACAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                               >  1:7163670/1‑101 (MQ=255)
         ccccTCCCCCCAATAGTCGTAACTGAATTTCTGAAGCAAGCCGGGGTCTTTTCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                               <  2:2921290/100‑1 (MQ=21)
          cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCAt                                                                                              <  2:5333207/101‑1 (MQ=255)
            cTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCt                                                                                            <  2:4273574/101‑1 (MQ=255)
             tCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                           <  1:7768614/101‑1 (MQ=255)
              cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTg                                                                                          <  1:940499/101‑1 (MQ=255)
               gccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGAATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGc                                                                                         >  1:3901764/1‑101 (MQ=255)
                 cgcAATATGGGGAACTTGATTTCTGAAGCCAGCCGGGGGCTCTGCCCCCGGGCCCCCGGGATATTTGAGGGAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                       <  2:8723807/101‑1 (MQ=37)
                 cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                       <  2:4030358/101‑1 (MQ=255)
                  gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                      >  1:2235948/1‑101 (MQ=255)
                   cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                     >  2:7401018/1‑101 (MQ=255)
                   cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                     >  1:5868209/1‑101 (MQ=255)
                   cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                     <  2:4846841/101‑1 (MQ=255)
                    aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                    >  2:2993864/1‑101 (MQ=255)
                     atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                   >  2:9062615/1‑101 (MQ=255)
                     atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                   <  1:1415705/101‑1 (MQ=255)
                      tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAat                                                                                  <  1:8936290/101‑1 (MQ=255)
                        tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                                <  1:4041024/101‑1 (MQ=255)
                             aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                          <  2:5257481/101‑1 (MQ=255)
                                ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCGGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                       >  1:6448315/1‑101 (MQ=255)
                                ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                       >  2:6500851/1‑101 (MQ=255)
                                 tGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACCCGCCCCTTTCATCTTGCCCCAAATATCCC.gggggg                                                                      >  2:1733794/1‑100 (MQ=255)
                                 tGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATCAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gggggg                                                                      >  1:3183012/1‑100 (MQ=255)
                                  gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                     >  1:3332336/1‑101 (MQ=255)
                                  gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                     <  2:2498947/101‑1 (MQ=255)
                                   atgtCTGAAGCAAGCCGGGGGCTCTGCCCCCGGCCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                                    <  1:2050854/98‑1 (MQ=255)
                                   aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                                    >  1:8402612/1‑101 (MQ=255)
                                    tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                   >  1:43596/1‑101 (MQ=255)
                                    tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                   <  2:4407981/101‑1 (MQ=255)
                                    tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                   >  1:5292204/1‑101 (MQ=255)
                                       cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                <  2:2398387/101‑1 (MQ=255)
                                       cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                <  1:2859057/101‑1 (MQ=255)
                                         gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGc                                                              >  1:4535579/1‑101 (MQ=255)
                                         gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGc                                                              <  1:4965421/101‑1 (MQ=255)
                                          aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGcc                                                             >  2:1608991/1‑101 (MQ=255)
                                             caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                          >  2:2313274/1‑101 (MQ=255)
                                              aaggCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                         >  1:8812904/1‑101 (MQ=255)
                                              aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGCGAATTTGCCGCCa                                                         >  2:5479498/1‑101 (MQ=255)
                                               agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                        >  1:6306793/1‑101 (MQ=255)
                                                  cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                     >  2:3480720/1‑101 (MQ=255)
                                                  cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                     >  2:837361/1‑101 (MQ=255)
                                                   gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                    >  2:4150419/1‑101 (MQ=255)
                                                     gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                  <  1:7038277/101‑1 (MQ=255)
                                                       gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATGTCCC.GGGGGTGAATTTGCCGTCAGGCAAaga.g                                               >  1:6103908/1‑101 (MQ=255)
                                                       gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga.g                                               >  2:1964008/1‑101 (MQ=255)
                                                         tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.ggg                                             >  2:6963918/1‑101 (MQ=255)
                                                            ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGA.ggggggc                                         >  1:7876296/9‑99 (MQ=25)
                                                            gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGc                                          <  2:4507210/101‑1 (MQ=255)
                                                            gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTAATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGc                                          <  1:4713724/101‑1 (MQ=255)
                                                             tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGA.ggggggca                                        <  1:4819446/94‑4 (MQ=25)
                                                             tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGA.ggggggca                                        <  2:8846147/94‑4 (MQ=25)
                                                             gttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGA.GGGGGCAg                                        <  1:5172488/93‑1 (MQ=2)
                                                             cccccGGACCCCAGGGATATTTGAGGCAGGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCACAGA.GGGGGCa                                         >  1:6532028/1‑101 (MQ=255)
                                                               cccGGCCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGc                                       <  2:5136331/101‑1 (MQ=255)
                                                                ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCt                                      >  2:6022236/1‑101 (MQ=255)
                                                                  ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTcc                                    >  1:5546334/1‑101 (MQ=255)
                                                                     cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTccccc                                 <  2:824489/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCt                                <  1:2101816/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCt                                <  2:7044129/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGACTTTGCCGTCAGGCAAAGC.GGGGGCAGCTCCCCCTGc                              >  1:2982147/1‑101 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGc                              >  2:7126634/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGccc                            >  1:1250535/1‑101 (MQ=255)
                                                                           ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAGGGGGGCAGCGCCCCCttttcg                           >  1:5401536/1‑96 (MQ=17)
                                                                           ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGcccc                           >  1:7446384/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGGCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                          <  1:8297775/101‑1 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                          >  1:1702059/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                          <  2:119440/101‑1 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                          >  1:2760400/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCgg                         >  1:251608/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGc                        <  1:3569005/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCt                       >  2:761154/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgag                    >  2:2843964/1‑101 (MQ=255)
                                                                                   taGGCAAGGTGAAACTCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGTGTGAATTTGCCGTCAGGCAAATA.GGGGGCAGCTCCCCCTGCCCCGGCTgagg                   <  2:1448414/100‑1 (MQ=38)
                                                                                   gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagg                   <  2:8980495/101‑1 (MQ=255)
                                                                                   gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagg                   <  1:4174742/101‑1 (MQ=255)
                                                                                    aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgaggg                  <  1:8540004/101‑1 (MQ=255)
                                                                                    aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgaggg                  >  2:7784586/1‑101 (MQ=255)
                                                                                        aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc              >  2:3619209/1‑101 (MQ=255)
                                                                                        aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc              >  2:5789627/1‑101 (MQ=255)
                                                                                          gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg            <  1:6238887/101‑1 (MQ=255)
                                                                                           aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa           <  2:1352824/101‑1 (MQ=255)
                                                                                            tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCAGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa          >  1:8136919/1‑101 (MQ=255)
                                                                                             gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc         >  2:6189184/1‑101 (MQ=255)
                                                                                             gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc         <  1:1003366/101‑1 (MQ=255)
                                                                                              aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt        >  1:2361510/1‑101 (MQ=255)
                                                                                              aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt        >  2:6275976/1‑101 (MQ=255)
                                                                                                acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg      <  2:1018761/101‑1 (MQ=255)
                                                                                                    gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc  >  1:2692683/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
CGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAC  >  NC_014034/2950615‑2950815

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG