Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,765 A→C 8.2% intergenic (+108/‑52) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7650A→C 8.2% 95.1 ‑0.3 ‑0.4478intergenic (+108/‑52)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (22/17):  ref base (231/208):  total (253/225)
Fisher's exact test strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.97e-01

Only 100 of 524 total aligned reads displayed.

CGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGCGAAAG  >  NC_014034/2950665‑2950865
                                                                                                      |                                                                                                     
  ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCaaa                                                                                                     <  2:4492046/101‑1 (MQ=255)
    ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAaga                                                                                                   <  1:8712855/101‑1 (MQ=255)
    gcctctGCCCCCCGACCCCCGGGATATTTGAGGCAAGATAAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAaga                                                                                                   <  2:2915364/99‑1 (MQ=255)
        gcttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGA.ggggg                                                                                             <  1:2677662/91‑1 (MQ=25)
         cttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGA.gggggg                                                                                            >  2:2549234/10‑100 (MQ=25)
          ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGA.ggggggc                                                                                           >  2:3547890/9‑99 (MQ=25)
          gcttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAA.GGCCAAGA.GGGGGCa                                                                                           >  2:7121325/10‑101 (MQ=2)
           tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGA.ggggggca                                                                                          <  2:8887384/94‑4 (MQ=25)
           gttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAA.GGCCAAGA.GGGGGCAg                                                                                          <  1:5172488/93‑1 (MQ=2)
             cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGc                                                                                         <  1:6250318/101‑1 (MQ=255)
              ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCt                                                                                        >  2:1727853/1‑101 (MQ=255)
               cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTc                                                                                       >  1:552594/1‑101 (MQ=255)
                 gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTccc                                                                                     >  1:4866731/1‑101 (MQ=255)
                  ccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGGCCAAA.GGCAAAGA.GGGGGCAGGGcccc                                                                                    >  1:8937715/2‑101 (MQ=2)
                  aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTcccc                                                                                    <  1:4009886/101‑1 (MQ=255)
                   cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTccccc                                                                                   <  1:780358/101‑1 (MQ=255)
                    ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCt                                                                                  <  1:2101816/101‑1 (MQ=255)
                     cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTg                                                                                 >  2:8799262/1‑101 (MQ=255)
                     cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTg                                                                                 <  2:4311338/101‑1 (MQ=255)
                      ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGc                                                                                <  2:8220819/101‑1 (MQ=255)
                        gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGccc                                                                              >  2:5453743/1‑101 (MQ=255)
                        gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGccc                                                                              >  1:1880683/1‑101 (MQ=255)
                         ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGAGGGGGGCAGCGCCCCCttttcg                                                                             >  1:8042551/1‑96 (MQ=17)
                         ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AA.GGCCAAGAGGGGGGCAGCGCCCCCttttcg                                                                             >  1:5401536/1‑96 (MQ=17)
                         ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGcccc                                                                             <  2:8366962/101‑1 (MQ=255)
                         ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGcccc                                                                             <  2:2236039/101‑1 (MQ=255)
                          gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                                                                            >  2:761909/1‑101 (MQ=255)
                          gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                                                                            >  2:1003842/1‑101 (MQ=255)
                           atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCgg                                                                           >  1:251608/1‑101 (MQ=255)
                            tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGc                                                                          <  2:5322927/101‑1 (MQ=255)
                            tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGc                                                                          >  2:4211348/1‑101 (MQ=255)
                            tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGc                                                                          <  1:3569005/101‑1 (MQ=255)
                            tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGACCCGGc                                                                          >  1:4391691/1‑101 (MQ=255)
                              tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTg                                                                        >  1:3918067/1‑101 (MQ=255)
                               ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTga                                                                       <  1:1472750/101‑1 (MQ=255)
                                tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                      >  1:4734538/1‑101 (MQ=255)
                                tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                      >  2:8696955/1‑101 (MQ=255)
                                tGAGGCAAGATGAAACACGCCCCTTTCATCTTCCCCCAAATATCCC.GGGGGAGAATTTGTCGTCA.GGCAAAGA.GGGGACAGCTCTCCCTGCACCGTCTgag                                                                      >  2:5188112/1‑101 (MQ=25)
                                 gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                                     <  1:6172103/101‑1 (MQ=255)
                                 gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                                     <  1:4120818/101‑1 (MQ=255)
                                  aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                                    >  2:7929665/1‑101 (MQ=255)
                                    gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                                  >  2:2849213/1‑101 (MQ=255)
                                    gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                                  >  2:6489482/1‑101 (MQ=255)
                                    gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                                  >  2:6069077/1‑101 (MQ=255)
                                       aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                               >  2:1360096/1‑101 (MQ=255)
                                           gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                           >  2:6189184/1‑101 (MQ=255)
                                           gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                           <  1:1003366/101‑1 (MQ=255)
                                           gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                           >  1:1837187/1‑101 (MQ=255)
                                            aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                                          <  1:2859574/101‑1 (MQ=255)
                                            aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                                          <  1:7117459/101‑1 (MQ=255)
                                            aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                                          >  1:7445661/1‑101 (MQ=255)
                                             aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                                         >  2:3954173/1‑101 (MQ=255)
                                                acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                                      <  1:2372437/101‑1 (MQ=255)
                                                  gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                                    <  2:6306553/101‑1 (MQ=255)
                                                   ccccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                                                   >  1:3415666/1‑101 (MQ=255)
                                                     ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                                                 <  2:8402249/101‑1 (MQ=255)
                                                      cTTTCATCTGGCCCCAAATATCCC.GGGGGTGAATTTGCCGGCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                                <  1:2227060/101‑1 (MQ=255)
                                                       tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                               >  2:7411919/1‑101 (MQ=255)
                                                        ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                              <  2:5724220/101‑1 (MQ=255)
                                                        ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                              >  1:3063934/1‑101 (MQ=255)
                                                        ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                              >  2:14579/1‑101 (MQ=255)
                                                        ttCATCTTGCCCAAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGTCCCGGCTGTGGCAGGCGGGACTGGAAACCCTGac                                              >  2:5996593/1‑100 (MQ=25)
                                                         tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                             >  2:7782362/1‑101 (MQ=255)
                                                          cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                            >  2:903544/1‑101 (MQ=255)
                                                            tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                          <  2:1174576/101‑1 (MQ=255)
                                                             cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                         >  1:1284780/1‑101 (MQ=255)
                                                               tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                       <  1:1597490/101‑1 (MQ=255)
                                                               tGCCCCAAATATCCC.GGGGGTGAATTTGCCGCCA.GGCAAAGA.GGGGGCAGCCCCCCCTGCCCCGGCTGAGGGCGGCGGAACTGGAAACCTTGAAGACCGGc                                       >  2:4105392/1‑101 (MQ=37)
                                                                gCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGcc                                      <  1:5332105/101‑1 (MQ=255)
                                                                 ccccAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                                     <  1:4614675/101‑1 (MQ=255)
                                                                    cAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                                  >  2:4542565/1‑101 (MQ=255)
                                                                    cAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                                  >  2:5092867/1‑101 (MQ=255)
                                                                     aaaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                                 >  1:7012042/1‑101 (MQ=255)
                                                                     aaaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                                 >  1:6786081/1‑101 (MQ=255)
                                                                     aaaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                                 <  2:1950813/101‑1 (MQ=255)
                                                                      aaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                                <  1:2445751/101‑1 (MQ=255)
                                                                      aaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                                >  2:6909207/1‑101 (MQ=255)
                                                                      aaTATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                                <  1:8361611/101‑1 (MQ=255)
                                                                       atatCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                               >  1:8538979/1‑101 (MQ=255)
                                                                        tatCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                              >  2:8275662/1‑101 (MQ=255)
                                                                        tatCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                              >  1:925676/1‑101 (MQ=255)
                                                                        tatCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                              >  2:4583144/1‑101 (MQ=255)
                                                                        tatCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                              >  2:4388425/1‑101 (MQ=255)
                                                                         acccc.GGGGGGGAATTTTCCGGGAGGGAAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                              <  1:5789862/99‑1 (MQ=255)
                                                                           ccc.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                           <  1:117826/101‑1 (MQ=255)
                                                                                ggggTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc                       <  2:2811899/101‑1 (MQ=255)
                                                                                ggggTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc                       <  2:5867974/101‑1 (MQ=255)
                                                                                 gggTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg                      >  1:7538471/1‑101 (MQ=255)
                                                                                  ggTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgc                     >  1:2792964/1‑101 (MQ=255)
                                                                                      aaTTTGCCGTCA.GGCAAAGA.GGGGGCGGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTAAAGACCCGCCTGATCGAAGAGGCCCGCGCGa                 >  1:7964071/1‑101 (MQ=255)
                                                                                        tttGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc               >  2:3688061/1‑101 (MQ=255)
                                                                                        tttGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc               <  2:1250503/101‑1 (MQ=255)
                                                                                              gTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt         >  2:3001305/1‑101 (MQ=255)
                                                                                              gTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt         >  1:8194224/1‑101 (MQ=255)
                                                                                               tCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg        <  2:2080010/101‑1 (MQ=255)
                                                                                               tCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg        <  2:4851082/101‑1 (MQ=255)
                                                                                               gga.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg        <  2:3441886/99‑1 (MQ=255)
                                                                                                   ggCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGCGa     >  2:2439638/1‑101 (MQ=255)
                                                                                                    gCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGCGaa    <  2:3234607/101‑1 (MQ=255)
                                                                                                      aaaGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGCGAAAg  >  1:6485432/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
CGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCA.GGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGCGAAAG  >  NC_014034/2950665‑2950865

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG