Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,499 A→G 100% E123E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,4990A→G100.0% 1612.6 423E123E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (257/166):  ref base (0/0):  total (257/166)

Only 100 of 433 total aligned reads displayed.

GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596
                                                                                                  |                                                                                                 
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTTCAAAATGTCATGGAGgc                                                                                                 >  1:5334688/1‑101 (MQ=255)
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 <  2:2894523/101‑1 (MQ=255)
    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             <  1:8310155/101‑1 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            <  2:2291930/101‑1 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            >  1:5014307/1‑101 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            <  2:6892606/101‑1 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         >  2:3577838/1‑101 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         <  2:5322226/101‑1 (MQ=255)
         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        >  1:611474/1‑101 (MQ=255)
          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACg                                                                                       <  1:6484166/101‑1 (MQ=255)
           ggTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCggggcggg                                                                                      >  1:4238545/1‑95 (MQ=255)
            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAACATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  2:5704900/1‑101 (MQ=255)
             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    >  1:4499136/1‑101 (MQ=255)
              aaCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgagatcggaag                                                                                   >  2:3072833/1‑91 (MQ=38)
              aaCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTc                                                                                   >  2:6666305/1‑101 (MQ=255)
                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTgg                                                                                >  2:1815321/1‑101 (MQ=255)
                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTgg                                                                                <  2:8020495/101‑1 (MQ=255)
                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTga                                                                                <  1:8920361/101‑2 (MQ=255)
                  cgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTggg                                                                               >  1:3642276/1‑101 (MQ=255)
                  cgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTggg                                                                               <  1:3783210/101‑1 (MQ=255)
                    gTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGcc                                                                             >  1:4061302/1‑101 (MQ=255)
                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       >  2:2059128/1‑101 (MQ=255)
                           cccAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      <  2:6151970/100‑1 (MQ=255)
                            ccAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCg                                                                     <  2:7257223/101‑1 (MQ=255)
                              atccTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   <  2:5444199/99‑1 (MQ=255)
                              aaCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   <  1:1249294/101‑1 (MQ=255)
                              aaCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   <  2:818984/101‑1 (MQ=255)
                                ccTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCg                                                                 >  2:3340814/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:5378935/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:2598668/1‑101 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               >  1:1002858/1‑100 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            >  1:5979397/1‑101 (MQ=255)
                                     atctGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  2:2713304/98‑1 (MQ=255)
                                      taTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagggcg                                                           >  2:8427217/2‑96 (MQ=255)
                                      gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           >  1:4395152/1‑101 (MQ=255)
                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          >  1:1788215/1‑101 (MQ=255)
                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          >  2:7162285/1‑101 (MQ=255)
                                        tGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGaa                                                         <  1:1059942/101‑1 (MQ=255)
                                         ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTcagat                                                        >  1:2713386/1‑97 (MQ=255)
                                         ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAAc                                                        >  1:1287960/1‑101 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:6853706/101‑1 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  1:8953028/1‑101 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  1:629985/1‑101 (MQ=255)
                                             ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGCGCGAAGCGCt                                                    >  2:8925169/1‑101 (MQ=255)
                                              tCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                   <  1:5659513/101‑1 (MQ=255)
                                               ccAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCg                                                  >  2:4541872/1‑101 (MQ=255)
                                                 agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                >  2:7047290/1‑101 (MQ=255)
                                                 agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                >  1:1314233/1‑101 (MQ=255)
                                                  gcagTTCAACCTCTGGAGCCACATGCCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  2:1919627/101‑1 (MQ=255)
                                                  gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  1:6598792/101‑1 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             >  2:5365408/1‑101 (MQ=255)
                                                     gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                            >  2:7654289/1‑101 (MQ=255)
                                                       tCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGc                                          >  1:7307764/1‑101 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCCGcc                                         >  2:2616765/1‑101 (MQ=255)
                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        >  2:4650509/1‑101 (MQ=255)
                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        >  2:2196733/1‑101 (MQ=255)
                                                          aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                       <  2:3843841/101‑1 (MQ=255)
                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      <  2:8369797/101‑1 (MQ=255)
                                                            ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                     <  1:7686523/101‑1 (MQ=255)
                                                            ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                     <  1:8427580/101‑1 (MQ=255)
                                                              ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                   <  1:484540/101‑1 (MQ=255)
                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACAGGCCGAGGTCGAACCGCTCACCCGCAGCCttagatc                                  >  1:2931388/1‑96 (MQ=255)
                                                               gggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                  <  2:170014/100‑1 (MQ=255)
                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGcc                                 <  1:2013542/101‑1 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                <  2:1571576/101‑1 (MQ=255)
                                                                  aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                               >  2:4269406/1‑101 (MQ=255)
                                                                   gCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAg                              >  1:4120889/1‑101 (MQ=255)
                                                                     cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                            <  1:3394252/101‑1 (MQ=255)
                                                                       caTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCg                          >  1:3086867/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  2:4973372/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  2:7322958/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGAGCCGGCGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAACCGCTCGCCCGCAGCCTTCTGGCCAAggcggg                         >  1:1423536/1‑97 (MQ=255)
                                                                         tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTcaga                        >  2:4902468/1‑98 (MQ=255)
                                                                         tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                        <  1:8654562/101‑1 (MQ=255)
                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  2:9182945/1‑101 (MQ=255)
                                                                           aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                      <  1:7843507/101‑1 (MQ=255)
                                                                            ctaTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     >  2:8533894/3‑101 (MQ=255)
                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     <  2:3244493/101‑1 (MQ=255)
                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    >  2:1400124/1‑101 (MQ=255)
                                                                              aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                   >  1:1854446/1‑101 (MQ=255)
                                                                              aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                   <  1:7047575/101‑1 (MQ=255)
                                                                               tCCTGGAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                  <  1:7350617/101‑1 (MQ=255)
                                                                               tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                  <  1:5591773/101‑1 (MQ=255)
                                                                               tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                  >  2:5866720/1‑101 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 <  1:8246954/101‑1 (MQ=255)
                                                                                  tGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGAc               <  2:7566418/101‑1 (MQ=255)
                                                                                   gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa              <  1:7514531/101‑1 (MQ=255)
                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            >  1:9034646/1‑100 (MQ=255)
                                                                                      aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg           >  2:7929268/1‑99 (MQ=255)
                                                                                      aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGACCAAGGTCGGCATCGGCGACaagg           <  1:7162589/101‑3 (MQ=255)
                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         >  2:5592838/1‑101 (MQ=255)
                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         >  1:8198846/1‑101 (MQ=255)
                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        >  2:1221538/1‑101 (MQ=255)
                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        <  1:2378603/101‑1 (MQ=255)
                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        <  2:4013289/101‑1 (MQ=255)
                                                                                          gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       >  2:6630139/1‑101 (MQ=255)
                                                                                            cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg     >  1:1567194/1‑101 (MQ=255)
                                                                                             aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc    >  1:351989/1‑101 (MQ=255)
                                                                                             aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc    <  2:8774289/101‑1 (MQ=255)
                                                                                               ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt  >  1:8521913/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG