Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,962,711 A→G 100% K21E (AAG→GAG)  hisM → polar amino acid ABC transporter permease HisM

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,962,7110A→G100.0% 1626.4 433K21E (AAG→GAG) hisMpolar amino acid ABC transporter permease HisM
Reads supporting (aligned to +/- strand):  new base (204/228):  ref base (0/0):  total (204/229)

Only 100 of 451 total aligned reads displayed.

GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808
                                                                                                  |                                                                                                 
     gaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGcgcgg                                                                                            >  1:1002546/1‑100 (MQ=255)
      aTGGCGGGCGAAGCGCAACGGAAGGGCGCGGGCTGAGCGACAGCTGCACGCAGATCATCGCGGAGTACGCCTTCCCCCCCCCGGGCTATGGGGAGCGCGtg                                                                                           >  2:25328/1‑101 (MQ=255)
       tGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgc                                                                                          <  2:1181028/101‑1 (MQ=255)
       tGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgc                                                                                          <  1:3468448/101‑1 (MQ=255)
          cgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctgc                                                                                       >  2:5768394/1‑101 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      >  1:3600144/1‑101 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      <  2:3062232/101‑1 (MQ=255)
            ggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcg                                                                                     <  2:7923540/101‑1 (MQ=255)
              cgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGATCGCGTGCTGCcgcg                                                                                   >  2:3462596/1‑101 (MQ=255)
              cgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcgcg                                                                                   >  1:8058076/1‑101 (MQ=255)
               gAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCgcgc                                                                                  >  1:9136808/1‑101 (MQ=255)
                 aGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAg                                                                                <  1:2248438/101‑1 (MQ=255)
                   cgcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTg                                                                              <  1:3668644/101‑1 (MQ=255)
                    gcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGa                                                                             >  2:7654130/1‑101 (MQ=255)
                     cAACGGAAGGCCGCGGCATGAGCCACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGAc                                                                            <  2:8047438/101‑1 (MQ=255)
                      aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           >  2:4787377/1‑101 (MQ=255)
                        cGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTc                                                                         <  2:546724/101‑1 (MQ=255)
                        cGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTc                                                                         >  2:1372052/1‑101 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  1:5228619/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  2:5086073/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       >  2:839624/1‑101 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCACTGACTTCAc                                                                       >  1:6609750/1‑101 (MQ=255)
                           aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      >  2:2310853/1‑101 (MQ=255)
                           aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      >  1:1237383/1‑101 (MQ=255)
                             ggCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGct                                                                    <  1:607626/101‑1 (MQ=255)
                               ccGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGATCGCGTGCTGCCCCGCAGTGACTTCACGctct                                                                  >  2:5347952/1‑101 (MQ=255)
                               ccGCGACATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGctct                                                                  <  2:85839/101‑1 (MQ=255)
                                 gcgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGc                                                                >  2:8768901/1‑101 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               <  1:3144720/101‑1 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               <  2:4771000/101‑1 (MQ=255)
                                   ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              <  2:6803769/101‑1 (MQ=255)
                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             <  2:3298824/101‑1 (MQ=255)
                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             >  1:5427605/1‑101 (MQ=255)
                                        gAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAAt                                                         <  1:8884436/101‑1 (MQ=255)
                                            gACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     >  2:6720691/1‑101 (MQ=255)
                                               cgCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  <  2:2768208/100‑1 (MQ=255)
                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCACGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 >  1:8705688/1‑101 (MQ=255)
                                                 cTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGa                                                >  2:459883/1‑101 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              <  1:2736417/101‑1 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              >  1:2043847/1‑101 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              <  2:5233765/101‑1 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              >  1:1415313/1‑101 (MQ=255)
                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  1:5015174/101‑1 (MQ=255)
                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  2:100881/101‑1 (MQ=255)
                                                     aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            <  1:6367528/101‑1 (MQ=255)
                                                       gCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCt                                          >  1:39657/1‑101 (MQ=255)
                                                         aGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCg                                        >  2:3547/1‑101 (MQ=255)
                                                          gATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgg                                       >  1:5770873/1‑101 (MQ=255)
                                                           atcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCggg                                      >  2:2596172/1‑101 (MQ=255)
                                                            tcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgggg                                     <  1:1924683/101‑1 (MQ=255)
                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    >  1:384784/1‑101 (MQ=255)
                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    <  2:8208302/101‑1 (MQ=255)
                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    >  1:246539/1‑101 (MQ=255)
                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCt                                   >  2:3173544/1‑101 (MQ=255)
                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCt                                   <  2:6992714/101‑1 (MQ=255)
                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCGCGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  1:6141157/101‑1 (MQ=255)
                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  >  2:3937206/1‑101 (MQ=255)
                                                                cgcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGCGGCGGa                                 >  2:5586858/1‑101 (MQ=255)
                                                                 cgggATTACGGCTTCCCACGGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGAt                                <  2:2910375/99‑1 (MQ=255)
                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              >  1:5911419/1‑101 (MQ=255)
                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              >  1:7122511/1‑101 (MQ=255)
                                                                    gATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTg                             <  2:6146582/101‑1 (MQ=255)
                                                                     cgagctccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACACTGATCGGCTCGGGGCTGATCTgg                            <  1:7355571/95‑1 (MQ=255)
                                                                     aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTgg                            >  2:1929757/1‑101 (MQ=255)
                                                                     aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCCGAGCTgg                            >  2:6476988/1‑101 (MQ=255)
                                                                      ttACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCCGATCTGGa                           >  1:2944054/1‑101 (MQ=255)
                                                                       tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGaa                          >  1:7491355/1‑101 (MQ=255)
                                                                        aCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         <  1:6285/101‑1 (MQ=255)
                                                                         cGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACg                        <  2:6012877/101‑1 (MQ=255)
                                                                           ccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTc                      <  1:4865888/101‑1 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCt                     >  2:2382870/1‑101 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     <  2:154166/101‑1 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     <  1:774037/101‑1 (MQ=255)
                                                                             ttCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTa                    <  2:1829820/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  2:2235872/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGATGGTCTGGAACGGCTAt                   >  1:4129271/1‑101 (MQ=255)
                                                                              tCCGCTCGCTGGGCGATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  1:5604133/101‑1 (MQ=255)
                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  2:1711153/101‑1 (MQ=255)
                                                                               ccGCTCACTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  2:4489988/101‑1 (MQ=255)
                                                                                cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 <  1:4796538/101‑1 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttcc                <  1:8780410/101‑3 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                <  1:7593778/101‑1 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                >  1:1765125/1‑101 (MQ=255)
                                                                                   tcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCgg              >  1:4620176/1‑101 (MQ=255)
                                                                                    cgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGc             <  2:4332927/101‑1 (MQ=255)
                                                                                      ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATGGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           >  1:4341809/1‑101 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          <  2:8975884/101‑1 (MQ=255)
                                                                                        gggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGc         >  1:8528344/1‑101 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      <  2:412115/101‑1 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      >  2:7320775/1‑101 (MQ=255)
                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCCGGAACGGCTATTTCGGCACGCTgg      >  1:8277147/1‑101 (MQ=255)
                                                                                            tATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgc     <  2:7446067/101‑1 (MQ=255)
                                                                                            tATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCCGGCTGGTCCGGCACCTCTATTTCCGCAcccccgc     >  1:8434616/1‑95 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGGTCTGGAACGTCTATTTCGGCACGCTGgcg    >  2:9172234/1‑101 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    >  2:7982082/1‑101 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    >  1:7868017/1‑101 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    >  1:6816045/1‑101 (MQ=255)
                                                                                               ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  >  2:6338872/1‑101 (MQ=255)
                                                                                               ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  <  2:3792031/101‑1 (MQ=255)
                                                                                               ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  <  1:328012/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG