Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,969,954 C→G 100% intergenic (+105/+10) RCAP_rcc02782 → / ← RCAP_rcc02783 DegT/DnrJ/EryC1/StrS family aminotransferase/CoA‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,969,9540C→G100.0% 15.9 312intergenic (+105/+10)RCAP_rcc02782/RCAP_rcc02783DegT/DnrJ/EryC1/StrS family aminotransferase/CoA‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (291/5):  ref base (0/16):  total (291/21)
Fisher's exact test strand distribution p-value = 7.81e-23
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.04e-06

Only 100 of 368 total aligned reads displayed.

GCCGCCCCGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCGCCGCCGCCTCGGGG  >  NC_014034/2969856‑2970054
                                                                                                            |                                                                                                    
 ccgccCCGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.gccc                                                                                                   <  2:2313157/101‑1 (MQ=255)
  cgccCCGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.gcccc                                                                                                  <  1:4629993/101‑1 (MQ=255)
   gccCCGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.gccccc                                                                                                 <  2:8365069/101‑1 (MQ=255)
     ccgggTTGGGGGGGGAAAAGAGCGTATTTGGGCCAAGAAAAACCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCg                                                                                               <  1:820466/98‑1 (MQ=25)
      ccGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGc                                                                                              >  1:5144774/1‑101 (MQ=255)
       cGGTGCGGCGGGGGAAAGAGCGTATTTGGCCCAAGAAAAACCCCC.GGGGTTTTTTCTGGGAAAAAA..TACGC.GGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGcc                                                                                             <  2:7639318/101‑1 (MQ=25)
         gTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTc                                                                                           >  1:6448058/1‑101 (MQ=255)
           tCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTcac                                                                                         >  1:7936385/1‑101 (MQ=255)
            cggcggGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTcaca                                                                                        >  1:8539663/1‑101 (MQ=255)
              gggggggAAAGAACATGTTGTGAGCAAAAAAAACCCCC.GGGGTTTTTTCCTGGGAAAAA..TTAGG.GGGG.GGGTGG.GGGGGG...CAAACCCCCCCCGCCTCACAt                                                                                       <  2:7532836/99‑1 (MQ=255)
               cggGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATcc                                                                                     >  2:4460319/1‑101 (MQ=255)
               cggGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATcc                                                                                     >  1:1687238/1‑101 (MQ=255)
                gggggAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGATAAA..TACGC.TGGG.GGGTAT.GGGCGG...CAAA.GCCCCCCGCCTCACATccc                                                                                    >  2:9045018/1‑101 (MQ=255)
                 ggggAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATcccc                                                                                   >  2:6176469/1‑101 (MQ=255)
                   ggAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAg                                                                                 >  2:8312001/1‑101 (MQ=255)
                    ggaaGGGCGTTTTTGGGGCAAAAAAAAACCCC.GGGGTTTTTTTTTTGGAAAAA..AACGCGGGGG.GGGTGG.GGGGGG...AAAACCCCCCCCGCCTCACATCCCCa                                                                                  <  2:3505296/99‑1 (MQ=255)
                    gAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGc                                                                                >  1:4782139/1‑101 (MQ=255)
                    gAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGc                                                                                >  1:6784927/1‑101 (MQ=255)
                     aaaGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGcc                                                                               >  1:5587622/1‑101 (MQ=255)
                     aaaGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGcc                                                                               >  2:3790884/1‑101 (MQ=255)
                     aaaGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGcc                                                                               >  1:2350588/1‑101 (MQ=255)
                        gagCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCcgcg                                                                            >  1:8303345/1‑101 (MQ=255)
                         agCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCcgcgc                                                                           >  2:4030992/1‑101 (MQ=255)
                         agCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCcgcgc                                                                           >  1:1949198/1‑101 (MQ=255)
                           cGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGa                                                                         >  2:5409676/1‑101 (MQ=255)
                           cGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGa                                                                         >  2:8174503/1‑101 (MQ=255)
                            gTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTGACATCCCCAGCCGAGCGag                                                                        >  2:4291897/1‑100 (MQ=255)
                            gTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAt                                                                        >  1:6343798/1‑101 (MQ=255)
                            gTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAt                                                                        >  1:7511717/1‑101 (MQ=255)
                             tATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAtc                                                                       >  1:2834302/1‑101 (MQ=255)
                             tATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAtc                                                                       >  1:4444438/1‑101 (MQ=255)
                             tATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAtc                                                                       >  1:1474812/1‑101 (MQ=255)
                              tttgtggacAAAAAAAAACCCCTGGGTTTTTTTCTTGGAAAAAA..AGGGG.GGGGTGGGTAG.GGGGGA...AAAC.CCCCCCCGCCTCACATCCCCAGCCGCGCGAt                                                                        <  2:3267868/93‑1 (MQ=255)
                              aTTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGAtct                                                                      >  1:4099965/1‑101 (MQ=255)
                               ttggggGCAAAAAAAACCCCG.GGGGTTTTTTTTTGGAAAAAA..GGCGG.GGGG.GGGGTG.GGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGAtct                                                                      <  2:7186810/98‑1 (MQ=255)
                                 tGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGa                                                                   >  1:7883816/1‑101 (MQ=255)
                                     ccAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGAtttt                                                               >  1:7042313/1‑101 (MQ=255)
                                     ccAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGAttag                                                               >  2:2763225/1‑99 (MQ=255)
                                      cAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTc                                                              >  1:688223/1‑101 (MQ=255)
                                      cAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTc                                                              >  2:1504262/1‑101 (MQ=255)
                                        aaaaaaaaACCC.GGGGGTTTTTTTTGTGAAAAA..AAGGG.GGGG.GGGGGG.GGGGGGGGACACC.CCCCCCCGCCTCACATCCCCAGGCGCGCGATCTCGAtttt                                                               <  1:5448853/99‑1 (MQ=255)
                                        aGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCgg                                                            >  2:1997449/1‑101 (MQ=255)
                                         gAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCggg                                                           >  1:4871090/1‑101 (MQ=255)
                                         aaaaaaaCCCC.GGGGGGTTTTTTTTGGAAAAA..AAGGC.GGGG.GGGGGTGGGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCg                                                             <  2:894555/100‑1 (MQ=255)
                                           aaaaaCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCa                                                         >  1:2277829/1‑101 (MQ=255)
                                           aaaaaCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCa                                                         >  2:1874104/1‑101 (MQ=255)
                                            aaaaCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGccg                                                        >  2:2848112/1‑99 (MQ=255)
                                            aaaaCCCC.GCGGGTTTTTTTCGGGAAAAAATTGCGC.GGGG.GGGTGG.GGGGGC...CACC.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGc                                                          <  2:5242660/101‑1 (MQ=255)
                                             aaaCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGc                                                       >  2:1202475/1‑101 (MQ=255)
                                              aaCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTAGATTTTCGGGCAGCg                                                      >  2:8819811/1‑101 (MQ=255)
                                               caccc.GGGGGTTTTTTTCGGGAAAAA..AAGGCGGGGG.GGGTGG.GGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGc                                                       <  2:5089824/99‑1 (MQ=255)
                                               aCCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCgg                                                     >  1:8733843/1‑101 (MQ=255)
                                                cccc.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGt                                                    >  2:2361996/1‑101 (MQ=255)
                                                 ccc.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTc                                                   >  2:6670067/1‑101 (MQ=255)
                                                   c.GGGGTTTTTTTTAGGGAAAAA..AGCCG.GGGG.GGGTTG.GGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTcc                                                  <  2:2648672/101‑1 (MQ=255)
                                                   c.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCa                                                 >  1:2074256/1‑101 (MQ=255)
                                                   c.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCa                                                 >  1:3870610/1‑101 (MQ=255)
                                                    gggggTTTTTTTTTGGAAAAAA..ATGGG.GGGG.GGGGGG.GGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTcc                                                  <  1:5891723/100‑1 (MQ=255)
                                                      gggCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATc                                               >  2:5756179/1‑101 (MQ=255)
                                                         cTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATcacc                                            >  2:1393431/1‑101 (MQ=255)
                                                          tttttTTTGAAAAAAA..TGGGG.GGGG.GGGGGG.GGGGGG...AAAGCCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATcacc                                            <  1:7458725/101‑1 (MQ=255)
                                                          tttttCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCGACCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATcacca                                           >  1:9085957/1‑101 (MQ=255)
                                                             tttttGGGAAAAA..TGGGG.GGGG.GGGGTG.GGGGGG...CAAACCCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACcaca                                         <  1:4034377/98‑1 (MQ=255)
                                                             ttCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACaa                                        >  2:2502151/1‑101 (MQ=255)
                                                              tCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAg                                       >  2:2914801/1‑101 (MQ=255)
                                                              tCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAg                                       >  1:767815/1‑101 (MQ=255)
                                                               cTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGc                                      >  1:1235215/1‑101 (MQ=255)
                                                               cTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGc                                      >  1:4991852/1‑101 (MQ=255)
                                                                ttGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCt                                     >  1:3600853/1‑101 (MQ=255)
                                                                ttGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCt                                     >  1:8993047/1‑101 (MQ=255)
                                                                 tGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCtt                                    >  1:2870994/1‑101 (MQ=255)
                                                                 tGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCtt                                    >  2:6249647/1‑101 (MQ=255)
                                                                  gggAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCttc                                   >  1:2722341/1‑100 (MQ=255)
                                                                   ggAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CGAA.GCCGCCCGCCTCACATCCGCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGc                                  >  2:5908345/1‑101 (MQ=255)
                                                                      aaaa..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGcccc                               >  1:8426835/1‑100 (MQ=255)
                                                                      aaaa..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCccg                               >  2:5625364/1‑101 (MQ=255)
                                                                      aaaa..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCccg                               >  1:2807654/1‑101 (MQ=255)
                                                                       aaa..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCccgc                              >  1:8910867/1‑101 (MQ=255)
                                                                        aa..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCccgcc                             >  2:110853/1‑101 (MQ=255)
                                                                         a..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCccgccg                            >  1:1765493/1‑101 (MQ=255)
                                                                             aCGC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCgccgcc                          >  1:2387021/1‑101 (MQ=255)
                                                                               gcggggg.gggGGT.GGGGGGC..CAAC.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCgccgcc                          <  2:6383571/97‑1 (MQ=255)
                                                                               gC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGccgcccg                        >  2:3012667/1‑101 (MQ=255)
                                                                               gC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGccgcccg                        >  2:6998974/1‑101 (MQ=255)
                                                                               gC.TGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGccgcccg                        >  2:3695698/1‑101 (MQ=255)
                                                                                  tGGG.GGGTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGccgcccgcc                      >  2:6908333/1‑100 (MQ=255)
                                                                                   ggg.gggTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCcgcccgcgc                     >  2:6500225/1‑101 (MQ=255)
                                                                                     g.gggTGG.GGGGGG...AAGC.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCcgcccgcgccc                   <  2:4098332/101‑1 (MQ=255)
                                                                                       gggTAT.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCcgcccgcgcccg                  >  1:5987309/1‑101 (MQ=255)
                                                                                            t.GGGGGG...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGcgccg             >  2:4804660/1‑101 (MQ=255)
                                                                                            gggggggg...CACC.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCcgcgcccgcgcc              <  2:7922714/99‑1 (MQ=255)
                                                                                                gggg...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGcgccgccg          >  1:1062817/1‑101 (MQ=255)
                                                                                                  gg...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCgccgccgcc        >  2:3935101/1‑101 (MQ=255)
                                                                                                  gg...CAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCgccgccgcc        >  1:1001588/1‑101 (MQ=255)
                                                                                                   ggggccccccccccccGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGcgccgcc           <  1:397055/92‑1 (MQ=255)
                                                                                                   ggccccccccccccccGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGcgccgcc           <  2:8742239/92‑1 (MQ=255)
                                                                                                    gggccacccccccccGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGcgccgccg          <  2:8512803/93‑1 (MQ=255)
                                                                                                       cAAA.GCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCGCCGCCGCCTc      >  2:1733531/1‑101 (MQ=255)
                                                                                                         acccccccccGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCGCCGCCGCCTCg     <  2:4556845/98‑1 (MQ=255)
                                                                                                            cccccccGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCGCCGCCGCCTCgggg  <  2:6045547/101‑1 (MQ=255)
                                                                                                            |                                                                                                    
GCCGCCCCGGTTCGGCGGGGGAAAGAGCGTATTTGGGCCAAGAAAAAACCCC.GGGGCTTTTTCTTGGGAAAAA..TACGC.TGGG.GGGTAT.GGGGGG...CAAA.CCCCCCCGCCTCACATCCCCAGCCGCGCGATCTCGATTTTCGGGCAGCGGTCCATCACCACAAGCTTGCCCGCCGCCCGCGCCCGCGCCGCCGCCTCGGGG  >  NC_014034/2969856‑2970054

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG