Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 297,560 A→G 100% K12E (AAG→GAG)  RCAP_rcc00260 → alanine racemase domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034297,5600A→G100.0% 1567.4 418K12E (AAG→GAG) RCAP_rcc00260alanine racemase domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (247/171):  ref base (0/0):  total (247/171)

Only 100 of 439 total aligned reads displayed.

GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657
                                                                                                   |                                                                                                 
 ggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAgg                                                                                                >  2:5356934/1‑101 (MQ=255)
  gCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGc                                                                                               >  2:8246703/1‑101 (MQ=255)
   ccTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCg                                                                                              <  1:5099882/101‑1 (MQ=255)
    cTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGa                                                                                             >  2:1554380/1‑101 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           >  2:8663398/1‑101 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           <  1:1996182/101‑1 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           <  1:6820327/101‑1 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGGGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          >  1:5803342/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          <  2:1100814/101‑1 (MQ=255)
        gCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcg                                                                                         <  2:8954355/101‑1 (MQ=255)
        gCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcg                                                                                         >  2:8818040/1‑101 (MQ=255)
         cccGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgg                                                                                        <  2:2036461/101‑1 (MQ=255)
              ctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccg                                                                                    >  1:19647/2‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   >  1:2667186/1‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   >  2:5249852/1‑101 (MQ=255)
                cttttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcg                                                                                  >  1:4636593/1‑101 (MQ=255)
                cttttcttttTTGCCACCTTGTGCGCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcg                                                                                  >  2:4378211/1‑101 (MQ=255)
                   ttcttttTTGCCTCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               <  1:1573208/101‑1 (MQ=255)
                   ttcttttTTGCCGCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               <  1:5432803/101‑1 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  1:2308184/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  1:4413557/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               <  2:8076160/101‑1 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  1:3705531/1‑101 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              <  2:2615865/101‑1 (MQ=255)
                      ttttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgg                                                                            <  1:4071984/101‑1 (MQ=255)
                       tttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccggg                                                                           >  2:6342457/1‑101 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         >  1:660861/1‑101 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        <  2:73345/101‑1 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        <  1:8474317/101‑1 (MQ=255)
                           tGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcggcagcacatcg                                                                       <  2:6882081/101‑11 (MQ=255)
                            gCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAg                                                                      <  1:1769142/101‑1 (MQ=255)
                             ccACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAgg                                                                     <  1:7595253/101‑1 (MQ=255)
                              cACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggg                                                                    >  2:8288795/1‑101 (MQ=255)
                              cACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggg                                                                    >  2:467585/1‑101 (MQ=255)
                                ccTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggggg                                                                  <  1:3811876/101‑1 (MQ=255)
                                ccTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggggg                                                                  >  2:500001/1‑101 (MQ=255)
                                 cTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGc                                                                 <  2:681002/101‑1 (MQ=255)
                                  ttGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCg                                                                >  2:4600566/1‑101 (MQ=255)
                                  ttGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCg                                                                >  1:2152613/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  1:3408839/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  2:2786544/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  2:7104190/1‑101 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGTGATCGCCATGGGATTGCAGCAGATTCAGACCCGGATTGCGGAGGCGGCCCGGGCCGCCGCCCGGGCCGATGGGGCggc                                                              >  2:7199073/1‑100 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCggg                                                              >  2:3312079/1‑100 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGt                                                              >  1:125930/1‑101 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGc                                                            >  2:1412408/1‑101 (MQ=255)
                                        tCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCgg                                                          <  1:6681514/101‑1 (MQ=255)
                                         cGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGc                                                         <  2:5166612/101‑1 (MQ=255)
                                          gATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCt                                                        >  1:7432793/1‑101 (MQ=255)
                                          gATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCt                                                        >  2:6293101/1‑101 (MQ=255)
                                            ttGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGa                                                      <  2:5630980/101‑1 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCCGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCCCGGGCCGGGCCGAGGGGGCCGGGGGGCTGag                                                     >  2:2947650/1‑100 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAt                                                     >  2:7535451/1‑101 (MQ=255)
                                              gcgcCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATc                                                    <  2:6093615/101‑1 (MQ=255)
                                              gcgcCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATc                                                    <  1:100939/101‑1 (MQ=255)
                                                gccgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGc                                                  >  2:3388295/1‑101 (MQ=255)
                                                  cgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGCCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCg                                                <  2:3961638/101‑1 (MQ=255)
                                                    cAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtg                                              <  2:9137595/101‑1 (MQ=255)
                                                     aaaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtgt                                             >  1:2993226/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            <  1:1664790/101‑1 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGTAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGCGCGGCTGATCGCCGTGTCg                                           >  1:8377098/1‑101 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCg                                           <  1:3312183/101‑1 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGTCg                                           >  1:2617962/1‑101 (MQ=255)
                                                        ggAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGa                                          >  2:848601/1‑101 (MQ=255)
                                                        ggAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGa                                          >  1:6264744/1‑101 (MQ=255)
                                                         gagaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCgca                                         >  2:3996290/1‑99 (MQ=255)
                                                          agaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGCAg                                        >  1:3940454/1‑101 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    >  1:8621853/1‑101 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  1:5317655/101‑1 (MQ=255)
                                                                ccATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAg                                  >  2:7480590/1‑101 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 <  2:1704913/101‑1 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 <  1:8663783/101‑1 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAgc                                 >  2:2305518/1‑101 (MQ=255)
                                                                    gggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgc                              >  2:7944814/1‑101 (MQ=255)
                                                                    gggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGGCGAAGGTGCAgccgc                              >  1:1106393/1‑101 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            >  1:4905417/1‑101 (MQ=255)
                                                                        ttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          <  1:916335/101‑1 (MQ=255)
                                                                        tggCAGGAGATTCAGACCCGGATTGCGGAGGCGACGCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          <  1:2500281/99‑1 (MQ=255)
                                                                         tGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAg                         <  1:812017/101‑1 (MQ=255)
                                                                           cAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCg                       >  2:138010/1‑101 (MQ=255)
                                                                           cAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCg                       <  1:7153160/101‑1 (MQ=255)
                                                                            aGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCgg                      >  1:6960612/1‑101 (MQ=255)
                                                                              gagaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGt                    <  2:7771734/101‑1 (MQ=255)
                                                                              gagaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGt                    <  2:7011292/101‑1 (MQ=255)
                                                                                gaTTCAGACCCGGGTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGc                  <  1:5159456/101‑1 (MQ=255)
                                                                                gaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGc                  >  2:5508328/1‑101 (MQ=255)
                                                                                 aTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGGGcg                 >  2:6685025/1‑100 (MQ=255)
                                                                                  ttCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTg                >  1:7261438/1‑101 (MQ=255)
                                                                                  ttCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTg                >  2:178970/1‑101 (MQ=255)
                                                                                  ttCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAGCCGCCCGAGCGGGTGCTg                >  2:3641374/1‑101 (MQ=255)
                                                                                   tCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGCAGAAGGGGCAGCCGCCCGAGCGGGGGCTgg               >  1:7700343/1‑101 (MQ=255)
                                                                                     aGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCCCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGAGCTGGCg             >  1:5915582/1‑101 (MQ=255)
                                                                                      gACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCgg            >  2:2495760/1‑101 (MQ=255)
                                                                                        cccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTg          >  2:6500693/1‑101 (MQ=255)
                                                                                         ccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGGGc         >  1:884543/1‑101 (MQ=255)
                                                                                         ccGGATTGCGGAGGCGACCCGGGCCGCAGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGc         >  2:4965318/1‑101 (MQ=255)
                                                                                            gATTGCGGAGGCGCCCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCg      <  2:3015635/101‑1 (MQ=255)
                                                                                             aTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTTTCGAAGGGGAAGCCGCCCGAGCGGGTGCTGGCGGCGCTCGa     >  1:5646152/1‑101 (MQ=255)
                                                                                              ttGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGAt    <  2:1914562/101‑1 (MQ=255)
                                                                                                gCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCa  <  1:644998/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG