Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,033 C→A 1.1% intergenic (+79/+97) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0330C→A 1.1% 4.3 ‑0.8 ‑0.1543intergenic (+79/+97)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (258/278):  total (263/280)
Fisher's exact test strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.26e-01

Only 100 of 565 total aligned reads displayed.

GGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAG  >  NC_014034/3003933‑3004133
                                                                                                    |                                                                                                      
  tGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAg                                                                                                      <  2:6727199/101‑1 (MQ=255)
  tGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAg                                                                                                      <  2:6806044/101‑1 (MQ=255)
    cgcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.At                                                                                                   <  1:5719201/101‑1 (MQ=255)
     gcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATc                                                                                                  <  2:6164299/101‑1 (MQ=255)
      cgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATcc                                                                                                 <  2:8910064/101‑1 (MQ=255)
       gcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATccc                                                                                                <  1:7395079/101‑1 (MQ=255)
        cgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCg                                                                                               <  2:2783707/101‑1 (MQ=255)
         gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGTCGGCCAATTCCCCCCCCGGGATATTTGGACCAATGTGAAAGCAG.ATCCCgg                                                                                              >  1:4541529/1‑101 (MQ=255)
         gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCgg                                                                                              <  2:3764628/101‑1 (MQ=255)
          ggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGt                                                                                             <  1:720869/101‑1 (MQ=255)
           gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                            <  2:1248373/101‑1 (MQ=255)
            cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                           <  2:5718019/101‑1 (MQ=255)
            cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                           >  1:3039368/1‑101 (MQ=255)
            cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                           <  1:4948867/101‑1 (MQ=255)
             gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                          >  1:2963890/1‑101 (MQ=255)
                 tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                      >  1:2768951/1‑101 (MQ=255)
                 tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                      >  1:8168116/1‑101 (MQ=255)
                  cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                     <  1:3518893/101‑1 (MQ=255)
                    gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                   <  1:8536736/101‑1 (MQ=255)
                     aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGGCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAc                                                                                  <  1:171105/101‑1 (MQ=255)
                        gtaaGGGGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                               <  2:638034/99‑1 (MQ=255)
                        gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                               <  1:3153482/101‑1 (MQ=255)
                         cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                              >  1:934505/1‑101 (MQ=255)
                          aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                             <  1:5835693/101‑1 (MQ=255)
                          aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                             <  2:4709018/101‑1 (MQ=255)
                          aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                             <  2:4178850/101‑1 (MQ=255)
                          aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                             >  2:8631978/1‑101 (MQ=255)
                          aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                             <  2:3178134/101‑1 (MQ=255)
                           aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgc                                                                            <  2:7792505/101‑1 (MQ=255)
                              cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                         >  2:2671391/1‑101 (MQ=255)
                               ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                        >  2:1996758/1‑101 (MQ=255)
                               ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                        >  2:5412820/1‑101 (MQ=255)
                                 ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                      <  1:8367559/101‑1 (MQ=255)
                                 ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                      >  2:8090302/1‑101 (MQ=255)
                                 ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                      <  2:8734196/101‑1 (MQ=255)
                                 ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                      <  2:7306380/101‑1 (MQ=255)
                                      gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                 <  2:3333873/101‑1 (MQ=255)
                                         gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                              >  2:3876812/1‑101 (MQ=255)
                                          ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCACATATCCCgggg                                                             >  2:5192223/1‑101 (MQ=255)
                                          ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                             <  1:7558854/101‑1 (MQ=255)
                                          ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                             >  2:1151356/1‑101 (MQ=255)
                                          ccccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                       <  2:5550258/101‑6 (MQ=37)
                                           cccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagcccg                                                      >  1:7026628/1‑95 (MQ=37)
                                              ccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                         >  2:3482999/1‑101 (MQ=255)
                                              ccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                         <  2:706164/101‑1 (MQ=255)
                                               ctctTGGTCTGACGGCCAAATCACCCCCCGGGATATTTGGACCAAGGTGAAATCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                        <  1:5943719/101‑1 (MQ=255)
                                               ctctTGGCCTGACGGCCAATTCACCCCCCGGGAGATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                        >  1:6444682/1‑101 (MQ=255)
                                                tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                       >  1:5420441/1‑101 (MQ=255)
                                                 ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                      <  2:8840727/101‑1 (MQ=255)
                                                   tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                    <  1:8632364/101‑1 (MQ=255)
                                                   tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                    >  2:4324117/1‑101 (MQ=255)
                                                   tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                    >  1:7852565/1‑101 (MQ=255)
                                                   tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                    >  2:1703347/1‑101 (MQ=255)
                                                    ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                   >  1:5547984/1‑101 (MQ=255)
                                                         gACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                              >  1:6579464/1‑101 (MQ=255)
                                                         gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                              >  1:7101539/1‑101 (MQ=255)
                                                          aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                             <  1:8760052/101‑1 (MQ=255)
                                                           cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                            <  1:2462350/101‑1 (MQ=255)
                                                           cGGCCAATTCACCCCCCGGGATACTTGGACCAAGGTGTAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGCCg                                            >  2:9052544/1‑101 (MQ=255)
                                                              ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                         >  2:455069/1‑101 (MQ=255)
                                                                 aTTCACCTCCCGGGATGTTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                      <  1:8275795/101‑1 (MQ=255)
                                                                 aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                      <  1:4639324/101‑1 (MQ=255)
                                                                 aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                      <  2:8228790/101‑1 (MQ=255)
                                                                 aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                      <  1:2671473/101‑1 (MQ=255)
                                                                  ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                     <  2:2303668/101‑1 (MQ=255)
                                                                  ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                     >  1:3754041/1‑101 (MQ=255)
                                                                  ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCACGGGGGGAGTCCCGCGGGGACggggggtc                                     >  2:440213/1‑99 (MQ=255)
                                                                   tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                    >  2:6052535/1‑101 (MQ=255)
                                                                   tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                    >  1:2269052/1‑101 (MQ=255)
                                                                    ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                   >  2:7568943/3‑100 (MQ=18)
                                                                      ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.c                                >  1:164402/1‑101 (MQ=18)
                                                                        ccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cgc                              >  2:1409219/1‑99 (MQ=18)
                                                                        ccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccc                              >  1:6817266/1‑101 (MQ=18)
                                                                        ccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccc                              <  1:1351120/101‑1 (MQ=18)
                                                                          ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGCCGGGGGGCAGAg.ccccc                            >  1:5278632/1‑101 (MQ=255)
                                                                           cGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cccccc                           >  2:286175/1‑101 (MQ=18)
                                                                           cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cccccc                           <  2:2448746/101‑1 (MQ=17)
                                                                           cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccc                           <  1:6083469/101‑1 (MQ=255)
                                                                            gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                          >  1:3965519/1‑101 (MQ=255)
                                                                            gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                          >  2:2568458/1‑101 (MQ=255)
                                                                             ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccct                         >  1:2129917/1‑99 (MQ=17)
                                                                             ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctg                         >  2:648218/1‑101 (MQ=255)
                                                                              gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctc                        <  1:6194203/101‑4 (MQ=17)
                                                                              gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctc                        <  2:493580/101‑4 (MQ=17)
                                                                               atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctct                       >  1:3313985/1‑97 (MQ=18)
                                                                               atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcc                       >  1:8884274/1‑101 (MQ=255)
                                                                                tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctctt                      <  1:482196/101‑6 (MQ=17)
                                                                                tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGATGGGGGGCAGAg.cccccctgccc                      <  2:1075961/101‑1 (MQ=255)
                                                                                 atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                     >  2:3083756/1‑95 (MQ=18)
                                                                                 atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                     <  2:8017715/101‑7 (MQ=17)
                                                                                 atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                     >  2:3954906/1‑95 (MQ=18)
                                                                                 atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                     <  2:6426475/101‑7 (MQ=17)
                                                                                   ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccccc                   <  1:1894295/101‑1 (MQ=255)
                                                                                    tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCcccccctgcccccc                   >  2:1787627/1‑101 (MQ=255)
                                                                                     ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.cccccctgcacccctg                 >  1:9074883/1‑101 (MQ=255)
                                                                                          aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGcc            >  1:4270324/1‑101 (MQ=255)
                                                                                           aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCg           <  2:6624443/101‑1 (MQ=255)
                                                                                            ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGa          >  1:1007788/1‑101 (MQ=255)
                                                                                               gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcg       >  1:413368/1‑101 (MQ=255)
                                                                                                aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcgc      >  2:8422998/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
GGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAG  >  NC_014034/3003933‑3004133

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG