BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,048	C→A	11.2%	intergenic (+94/+82)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,048	0	C→A	11.2%	149.6 ‑0.2 0.0	541	intergenic (+94/+82)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (32/29): ref base (262/218): total (294/247)
Fisher's exact test strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 567 total aligned reads displayed.


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      >  1:6432860/1‑101 (MQ=255)


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      >  2:8255858/1‑101 (MQ=255)


  tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                                    >  1:8168116/1‑101 (MQ=255)


   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                   <  1:5797251/101‑1 (MQ=255)


    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                  <  1:2077636/101‑1 (MQ=255)


    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCCCTTTGCCCGGCCGGCCATTTCACCCCCCGGAAAATTGGGCCAAGGGTGAAACCAG.CCCCCGGTTGGCCttt                                                                                                   >  1:7310596/1‑101 (MQ=255)


      aGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGGCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAc                                                                                                <  1:171105/101‑1 (MQ=255)


      aGGGCAAGGCGGGGCGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAc                                                                                                <  1:1642128/101‑1 (MQ=255)


       gggCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                               <  2:7197304/101‑1 (MQ=255)


         gtaaGGGGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                             <  2:638034/99‑1 (MQ=255)


           aaGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                           <  2:4709018/101‑1 (MQ=255)


                ggggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                      >  2:7719472/1‑101 (MQ=255)


                ggggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                      <  2:7928215/101‑1 (MQ=255)


                 gggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                     >  1:2533962/1‑101 (MQ=255)


                  ggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    >  2:8657995/1‑101 (MQ=255)


                  ggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    <  2:7306380/101‑1 (MQ=255)


                  ggggCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    >  2:8090302/1‑101 (MQ=255)


                     gcgcTGCCCCCCTC.TTGGCGTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 <  1:7813856/101‑1 (MQ=255)


                     gcgcTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 <  1:6801499/101‑1 (MQ=255)


                      cgcTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                >  2:2739641/1‑101 (MQ=255)


                       gcTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                               <  2:3333873/101‑1 (MQ=255)


                          gCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                            >  2:454691/1‑101 (MQ=255)


                           ccccccTC.TTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                     <  2:5550258/101‑6 (MQ=37)


                            cccccTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                          <  1:9088938/101‑1 (MQ=255)


                            cccccTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                          <  2:6641663/101‑1 (MQ=255)


                               ccTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                                       <  2:706164/101‑1 (MQ=255)


                               ccTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                                       >  2:3482999/1‑101 (MQ=255)


                                    ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                   <  1:6267064/101‑1 (MQ=255)


                                    ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                   <  2:256668/101‑1 (MQ=255)


                                     tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                  <  1:947356/101‑1 (MQ=255)


                                         cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                              >  1:461615/1‑101 (MQ=255)


                                          tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                             >  1:5441818/1‑101 (MQ=255)


                                           gACGGCCAATTCACACCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                            <  2:1210575/101‑1 (MQ=255)


                                            aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                           <  2:8866213/101‑1 (MQ=255)


                                             cGGCCAATTCACCCCCCGGGATACTTGGACCAAGGTGTAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGCCg                                                          >  2:9052544/1‑101 (MQ=255)


                                                 cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                      >  2:8769526/1‑101 (MQ=255)


                                                 cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCACGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                      >  1:7145626/1‑101 (MQ=255)


                                                  aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                     >  1:3361459/1‑101 (MQ=255)


                                                   aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    >  2:5295947/1‑101 (MQ=255)


                                                   aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    <  1:2746830/101‑1 (MQ=255)


                                                   aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    >  1:2845819/1‑101 (MQ=255)


                                                    ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                   <  2:2303668/101‑1 (MQ=255)


                                                    ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                   >  1:3754041/1‑101 (MQ=255)


                                                      ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  2:2803813/99‑2 (MQ=18)


                                                      ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  1:8612536/99‑2 (MQ=18)


                                                      cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                 <  2:1736393/101‑1 (MQ=255)


                                                      cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                 >  1:4797197/1‑101 (MQ=255)


                                                      cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                 >  1:8697056/1‑101 (MQ=255)


                                                       cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                >  1:5924407/2‑99 (MQ=18)


                                                        ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                               >  2:2880086/1‑101 (MQ=18)


                                                         cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                              <  1:2992711/101‑1 (MQ=18)


                                                         cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                              <  1:4234450/101‑1 (MQ=255)


                                                           cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                            <  2:2427100/101‑1 (MQ=255)


                                                            ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                           <  1:8363446/101‑1 (MQ=255)


                                                             cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                          <  1:6083469/101‑1 (MQ=255)


                                                              gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATTCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                         >  2:1544547/1‑101 (MQ=255)


                                                                gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                       <  2:585727/101‑4 (MQ=17)


                                                                gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCTGAgcccccctgc                                       <  1:6573023/101‑1 (MQ=255)


                                                                gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                       <  1:7040773/101‑1 (MQ=255)


                                                                gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                       >  2:3204858/1‑101 (MQ=255)


                                                                 atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                      <  1:402906/101‑5 (MQ=17)


                                                                 atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                      >  1:8932854/1‑101 (MQ=255)


                                                                  tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                     <  1:5117215/101‑6 (MQ=17)


                                                                  tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     >  1:3053074/1‑101 (MQ=255)


                                                                  tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     >  2:6792763/1‑101 (MQ=255)


                                                                  tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     <  2:3895678/101‑1 (MQ=255)


                                                                   atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                    >  2:3083756/1‑95 (MQ=18)


                                                                   atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                    <  2:6426475/101‑7 (MQ=17)


                                                                     ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  >  1:7465351/1‑101 (MQ=255)


                                                                     ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  <  1:1894295/101‑1 (MQ=255)


                                                                      tGGACCAAGGTGAAAGCAG.AACCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccgcct                                 <  1:8800893/101‑1 (MQ=255)


                                                                          ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                             >  1:1288455/1‑101 (MQ=255)


                                                                            aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                           >  1:8520295/1‑101 (MQ=255)


                                                                            aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                           <  1:3876927/101‑1 (MQ=255)


                                                                             aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                          >  1:6928950/1‑101 (MQ=255)


                                                                              ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                         >  1:1007788/1‑101 (MQ=255)


                                                                              ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                         >  2:438714/1‑101 (MQ=255)


                                                                              ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                         >  2:1115370/1‑101 (MQ=255)


                                                                               gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        >  1:2301126/1‑101 (MQ=255)


                                                                                  aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  2:8422998/1‑101 (MQ=255)


                                                                                   aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgcc                    >  2:6155098/1‑100 (MQ=255)


                                                                                     gCAG.ATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                  <  1:440221/101‑1 (MQ=255)


                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:4180026/1‑101 (MQ=255)


                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:7549668/1‑101 (MQ=255)


                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  2:4315707/1‑101 (MQ=255)


                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  1:4916595/1‑101 (MQ=255)


                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               >  2:1876870/1‑101 (MQ=255)


                                                                                           tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt             >  2:2156191/1‑101 (MQ=255)


                                                                                            cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg            >  2:4935797/1‑101 (MQ=255)


                                                                                              cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          <  2:1318231/101‑1 (MQ=255)


                                                                                              cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          <  2:3749057/101‑1 (MQ=255)


                                                                                              cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt          <  1:2254334/101‑1 (MQ=255)


                                                                                                gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg        >  1:3265367/1‑101 (MQ=255)


                                                                                                 tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtttag       >  2:5116164/1‑99 (MQ=255)


                                                                                                   tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCGGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     >  2:3957684/1‑101 (MQ=255)


                                                                                                   tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     <  1:826439/101‑1 (MQ=255)


                                                                                                   tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     >  2:6493330/1‑101 (MQ=255)


                                                                                                      cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  1:8046503/1‑101 (MQ=255)


                                                                                                      cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  1:5592578/1‑101 (MQ=255)


                                                                                                      cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  2:5467104/1‑101 (MQ=255)


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTC.TTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG