Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,086 G→A 12.6% intergenic (+132/+44) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0860G→A12.6% 159.0 ‑1.9 ‑0.0477intergenic (+132/+44)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (31/29):  ref base (259/158):  total (290/187)
Fisher's exact test strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.19e-01

Only 100 of 535 total aligned reads displayed.

GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186
                                                                                                    |                                                                                                    
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   <  2:8699035/101‑1 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   <  1:3215982/101‑1 (MQ=255)
    gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                                                                  >  1:5923649/1‑101 (MQ=255)
     aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 >  1:6107137/1‑101 (MQ=255)
     aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 <  1:8760052/101‑1 (MQ=255)
     aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 <  1:3353432/101‑1 (MQ=255)
     aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 <  2:7448640/101‑1 (MQ=255)
      cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                                                                <  1:2462350/101‑1 (MQ=255)
          cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                            <  1:7351358/101‑1 (MQ=255)
          cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                            <  2:3911111/101‑1 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           >  1:2356008/1‑101 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           >  1:3862978/1‑101 (MQ=255)
            aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                          >  1:2845819/1‑101 (MQ=255)
             ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                         >  1:3754041/1‑101 (MQ=255)
              tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                        >  1:2269052/1‑101 (MQ=255)
              tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                        >  2:6052535/1‑101 (MQ=255)
              tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                        >  2:1122628/1‑101 (MQ=255)
               ccccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTGCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                                                       <  1:4259018/99‑2 (MQ=18)
               cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                       >  1:8697056/1‑101 (MQ=255)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                      >  2:2752946/1‑101 (MQ=255)
                 ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                                                     >  1:809234/1‑101 (MQ=18)
                  cccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCaccgcc                                                                                    >  2:6456643/1‑96 (MQ=21)
                  cccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                                                    <  1:2992711/101‑1 (MQ=18)
                  cccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                                                                    <  2:8299375/101‑1 (MQ=255)
                   ccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcgc                                                                                   >  2:1409219/1‑99 (MQ=18)
                     ccGGGATATTTGGACCAAGGTGAAAGCAGATCGCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                                                 <  2:6766404/101‑1 (MQ=255)
                      cGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                                                                <  2:2448746/101‑1 (MQ=17)
                       gggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                               >  2:2568458/1‑101 (MQ=255)
                        ggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                                              <  2:9189363/101‑3 (MQ=17)
                        ggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                                                              <  2:4576620/101‑1 (MQ=255)
                         gATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                                                             <  2:3799577/101‑4 (MQ=17)
                         gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCTCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                                             <  1:5409653/101‑1 (MQ=255)
                         gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCTGAgcccccctgc                                                                             <  1:6573023/101‑1 (MQ=255)
                         gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                                             >  2:1684705/1‑101 (MQ=255)
                          gtatTTGGCCCAAGTGGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                                                            <  2:2959661/100‑5 (MQ=17)
                          atatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                                                            >  1:8884274/1‑101 (MQ=255)
                           tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                           >  2:2214630/1‑96 (MQ=18)
                           tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                           <  1:5117215/101‑6 (MQ=17)
                           tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                                                           >  1:7013013/1‑101 (MQ=255)
                            atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                                          <  2:8017715/101‑7 (MQ=17)
                            atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                                          <  1:4002991/101‑7 (MQ=17)
                            atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                          >  1:4707956/1‑101 (MQ=255)
                            atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                          >  1:4931394/1‑101 (MQ=255)
                              ttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                                                        <  2:316894/101‑1 (MQ=255)
                               tGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgtccccctgcccccct                                                                       >  1:305300/1‑101 (MQ=255)
                                ggACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcacccctg                                                                      >  1:9074883/1‑101 (MQ=255)
                                  aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                    <  1:4832064/101‑1 (MQ=255)
                                  aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                    >  2:4921390/1‑101 (MQ=255)
                                  aCCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                    >  2:5429602/1‑101 (MQ=255)
                                   ccAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                                                                   >  1:8383543/1‑101 (MQ=255)
                                     aaGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                 <  1:1682787/101‑1 (MQ=255)
                                      aGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                                                                <  2:3243644/101‑1 (MQ=255)
                                       ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGTGGGCAGAGCCACCCTGCCCCCCGGGGGCCGa                                                               >  1:8579768/1‑101 (MQ=255)
                                       ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                               >  2:438714/1‑101 (MQ=255)
                                       ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                               >  1:1007788/1‑101 (MQ=255)
                                        gTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                                              <  2:9004149/101‑1 (MQ=255)
                                           aaaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                                                           >  1:8327738/1‑101 (MQ=255)
                                              gCAGATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                        <  1:440221/101‑1 (MQ=255)
                                              gCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                        >  1:2072472/1‑101 (MQ=255)
                                               cAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                       <  2:6900076/101‑1 (MQ=255)
                                                 gATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                     >  2:1876870/1‑101 (MQ=255)
                                                      cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                                <  1:190399/101‑1 (MQ=255)
                                                       ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                                               >  2:2852/1‑101 (MQ=255)
                                                        gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                              >  1:6335/1‑101 (MQ=255)
                                                        gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                              <  1:2739723/101‑1 (MQ=255)
                                                        gTTTGCATTACACCTTGGAGGAAATTGCCCGGGGGGAGTCCCGCGGTGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                              <  2:7711323/101‑1 (MQ=25)
                                                           tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                           <  2:721524/101‑1 (MQ=255)
                                                           tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                           >  1:8177507/1‑101 (MQ=255)
                                                              cTTTCACCTTGGCGCAAATAGCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc                                        <  2:706874/101‑1 (MQ=255)
                                                               tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAgac                                       >  2:2051405/1‑99 (MQ=255)
                                                               tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       >  2:5222187/1‑101 (MQ=255)
                                                               tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       >  2:2337758/1‑101 (MQ=255)
                                                                  cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct                                    >  2:5019606/1‑101 (MQ=255)
                                                                  cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct                                    >  1:1344226/1‑101 (MQ=255)
                                                                    ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                  >  1:4582316/1‑101 (MQ=255)
                                                                     cTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                 >  1:7660433/1‑101 (MQ=255)
                                                                      ttGGCGCAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc                                <  2:6367352/101‑1 (MQ=255)
                                                                      tgggCGCAAATATCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTc                                <  2:6497331/99‑1 (MQ=255)
                                                                        ggCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGa                              <  2:8850631/101‑1 (MQ=255)
                                                                             aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                         >  2:8144342/1‑101 (MQ=255)
                                                                             aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                         >  2:4498834/1‑101 (MQ=255)
                                                                             aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                         >  1:4212911/1‑101 (MQ=255)
                                                                              aaTAGCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                        <  2:8576517/101‑1 (MQ=255)
                                                                               atacCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                       <  1:7071350/101‑1 (MQ=255)
                                                                                taccccGGGGGGAGCCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                      <  1:7150789/98‑1 (MQ=255)
                                                                                 atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                     >  2:3971885/1‑101 (MQ=255)
                                                                                 atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                     >  2:5888825/1‑101 (MQ=255)
                                                                                 atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                     >  1:6827363/1‑101 (MQ=255)
                                                                                  cccgggggggAGTCCCGGGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGaa                    <  2:4191450/97‑1 (MQ=255)
                                                                                   cggggggggAGCCCCGGGGGGGGGGGGGCCAGCCCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                   <  1:1544594/98‑1 (MQ=255)
                                                                                       gggggAGCCCCGCGGGGGCGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCa               <  2:1213372/101‑1 (MQ=255)
                                                                                          ggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGc            >  1:6738551/1‑101 (MQ=255)
                                                                                          ggAGCCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGc            <  2:3439698/101‑1 (MQ=255)
                                                                                             gTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgg         >  2:5647337/1‑101 (MQ=255)
                                                                                               cccGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgggg       >  2:3595568/1‑101 (MQ=255)
                                                                                                 cgcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc     <  2:1485381/101‑1 (MQ=255)
                                                                                                  ggggggACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcg    <  1:6280862/99‑1 (MQ=255)
                                                                                                   gggggGCGGGGGGCAGAGCCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   <  1:5526805/100‑1 (MQ=255)
                                                                                                   cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   >  2:5506491/1‑101 (MQ=255)
                                                                                                    ggggACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgtc  >  1:7993716/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG