Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,011,286 T→A 82.1% intergenic (+85/+57) purK → / ← RCAP_rcc02822 phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,011,2860T→A82.1% 221.9 ‑0.2 ‑0.0370intergenic (+85/+57)purK/RCAP_rcc02822phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand):  new base (172/131):  ref base (36/30):  total (208/162)
Fisher's exact test strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 380 total aligned reads displayed.

TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379
                                                                                             |                                                                                             
 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggg                                                                                      >  2:437304/1‑101 (MQ=255)
  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggg                                                                                     >  1:2241356/1‑101 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                    >  1:35593/1‑101 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                    >  1:2808837/1‑101 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                    <  1:4956549/101‑1 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                   >  2:41394/1‑101 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                   <  1:7655573/101‑1 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                   >  1:1921265/1‑101 (MQ=255)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                   <  2:7517947/101‑1 (MQ=255)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                 >  2:5028050/1‑101 (MQ=255)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                 <  2:2299443/101‑1 (MQ=255)
        ccGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGAc                                                                               <  1:4834145/101‑1 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTg                                                                            <  1:1535563/101‑1 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTg                                                                            <  2:4528773/101‑1 (MQ=255)
            ggCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGc                                                                           >  2:2598103/1‑101 (MQ=255)
             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGcc                                                                          <  1:988998/101‑1 (MQ=255)
             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGcc                                                                          <  1:609378/101‑1 (MQ=255)
             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGcc                                                                          >  2:1979572/1‑101 (MQ=255)
              cAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCg                                                                         >  2:8272915/1‑101 (MQ=255)
              cAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCg                                                                         <  1:4321477/101‑1 (MQ=255)
                gCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCa                                                                       <  2:1197649/101‑1 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                     >  2:6287796/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                     >  1:860931/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                     >  1:3727322/1‑101 (MQ=255)
                     ggCTATCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGTGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGGaa                                                                  >  2:5867294/1‑101 (MQ=37)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAag                                                                 <  2:4747510/101‑1 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                 >  1:5529457/1‑101 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               >  2:7985651/1‑101 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               >  1:5481255/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                              <  1:2869524/101‑1 (MQ=255)
                          tCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggg                                                             >  1:3264916/1‑101 (MQ=255)
                          tCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggg                                                             >  2:360479/1‑101 (MQ=255)
                           cTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggg                                                            >  2:1372743/1‑101 (MQ=255)
                            tGCCCCGGCACCCGCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggggg                                                           <  1:7405966/101‑1 (MQ=38)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                          >  1:7059319/1‑101 (MQ=255)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                         <  2:5354587/101‑1 (MQ=255)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                         <  2:6668224/101‑1 (MQ=255)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                       <  1:5918363/101‑1 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                   <  2:2216446/101‑1 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                   >  1:6245356/1‑101 (MQ=255)
                                    cACCCCCGAGGAAATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGCGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                   >  1:8164205/1‑101 (MQ=37)
                                       ccccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccccc                                                >  2:3712968/1‑101 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              <  1:6326382/101‑1 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              <  2:5529250/101‑1 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              <  2:8637948/101‑1 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              >  2:8443867/1‑101 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             <  1:1729260/101‑1 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             <  2:3215630/101‑1 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             <  2:4608802/101‑1 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                            >  2:2839869/1‑101 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                            >  2:8100170/1‑101 (MQ=255)
                                             ggATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGccc                                          >  2:7959887/1‑101 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                         <  2:2861839/101‑1 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                         >  2:6352177/1‑101 (MQ=255)
                                               atatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCgg                                        <  1:8798275/101‑1 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                      >  1:6736094/1‑101 (MQ=255)
                                                  tttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTc                                     >  2:7917364/1‑101 (MQ=255)
                                                  ttgccGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTc                                     <  1:2118781/98‑1 (MQ=37)
                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                   <  2:8750928/101‑1 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 <  2:5462923/101‑1 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 >  2:7682536/1‑101 (MQ=255)
                                                       gCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGc                                <  1:2019386/101‑1 (MQ=255)
                                                       gCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGc                                <  1:2481040/101‑1 (MQ=255)
                                                        ccAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCa                               <  2:5329338/101‑1 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                              >  2:689052/1‑101 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                              >  1:7333925/1‑101 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             >  1:1160729/1‑101 (MQ=255)
                                                           aGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggg                            >  1:3081581/1‑101 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          >  2:5234262/1‑101 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          <  1:7946381/101‑1 (MQ=255)
                                                              tGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         <  2:6167072/101‑1 (MQ=255)
                                                              tGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         >  2:7374248/1‑101 (MQ=255)
                                                              tGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         <  1:46145/101‑1 (MQ=255)
                                                               gAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                        >  1:5208700/1‑101 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                      <  1:1812278/101‑1 (MQ=255)
                                                                  aacaacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                     >  1:1091756/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  2:268056/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  2:4018548/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  2:2043156/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  1:7534053/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGACCAGCCGc                    >  2:9013464/1‑101 (MQ=37)
                                                                    caacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCt                   >  1:2747512/1‑101 (MQ=255)
                                                                       cGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCa                <  1:4529380/101‑1 (MQ=255)
                                                                        gCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCACTGCaa               >  2:8163081/1‑101 (MQ=37)
                                                                        ccccTTTCGCCTTGGGACAAATAG.CCCGGGGGGAGCCTTGCCGCAGGCAAGGGGGGGGCAGCGCCCCCCTTCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               <  1:4112751/100‑1 (MQ=25)
                                                                        ccccTTCCCCCTGGGGAC.AAATATCCGGGGGGGAGACTGGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGC.CACGGCAGGGCCAGCCGCTGCAAg              <  2:7059034/100‑1 (MQ=21)
                                                                          ccTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAgg             >  2:3311018/1‑101 (MQ=255)
                                                                            tttCACCTTGGGAC.AAATATCCCGGGGGGAGCCTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc           <  2:1090316/101‑1 (MQ=37)
                                                                             ttCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcg          >  1:4714083/1‑101 (MQ=255)
                                                                             ttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGGCGCGGCAAgggcc          >  1:2925799/1‑98 (MQ=25)
                                                                             ttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcg          >  1:3920489/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc       >  1:4873667/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc       >  2:3431694/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCCGCAAGGCCgcgc       >  1:9104595/1‑101 (MQ=37)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    <  1:5336582/101‑1 (MQ=255)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    >  1:3655244/1‑101 (MQ=255)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    <  2:1765362/101‑1 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAGGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   >  2:6756690/1‑101 (MQ=37)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   <  1:5586937/101‑1 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   <  1:8334596/101‑1 (MQ=255)
                                                                                             |                                                                                             
TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG