Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,042,953 G→T 100% W217L (TGG→TTG)  thiD → phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,042,9530G→T100.0% 1545.9 404W217L (TGG→TTG) thiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  new base (245/159):  ref base (0/0):  total (245/159)

Only 100 of 419 total aligned reads displayed.

CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCG  >  NC_014034/3042855‑3043050
                                                                                                  |                                                                                                 
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGc                                                                                                >  1:1360280/1‑101 (MQ=255)
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGc                                                                                                <  2:7849988/101‑1 (MQ=255)
  aTCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCg                                                                                               <  1:5756446/101‑1 (MQ=255)
   tCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGa                                                                                              >  1:5920996/1‑101 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                           >  2:6941235/1‑101 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                           >  2:3113023/1‑101 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                           <  2:1180597/101‑1 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                           <  2:8837754/101‑1 (MQ=255)
       ctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCa                                                                                          >  2:1466361/1‑101 (MQ=255)
       ctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCa                                                                                          <  1:1440048/101‑1 (MQ=255)
          ctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCt                                                                                       >  2:622985/1‑101 (MQ=255)
          ctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCt                                                                                       >  1:8935164/1‑101 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    >  1:1830731/1‑101 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    <  2:1073453/101‑1 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    >  2:6627903/1‑101 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    >  1:4214599/1‑101 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    >  1:4750048/1‑101 (MQ=255)
              aTGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGcc                                                                                   >  2:4276812/1‑101 (MQ=255)
                   gcgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCAt                                                                              >  1:6432685/1‑101 (MQ=255)
                   gcgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCAt                                                                              <  2:1258834/101‑1 (MQ=255)
                      ggCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATccc                                                                           <  1:3908770/101‑1 (MQ=255)
                        cGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         >  1:5377181/1‑101 (MQ=255)
                        cGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         <  2:4033475/101‑1 (MQ=255)
                         gAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGa                                                                        >  1:4545978/1‑101 (MQ=255)
                          aGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGAt                                                                       >  2:3250838/1‑101 (MQ=255)
                            gTCTGGTGCGAGGCGTCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCCCATCCCCGATCt                                                                     <  2:6696990/101‑1 (MQ=255)
                              cTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCACCGATCTgc                                                                   >  2:340809/1‑101 (MQ=255)
                               tGGTTCGGGGCGCCGCGGGTCGAGACGAAAAACCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcg                                                                  <  1:2961913/101‑1 (MQ=255)
                               tGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcg                                                                  >  2:2477060/1‑101 (MQ=255)
                                ggatCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcgc                                                                 >  2:8005447/4‑101 (MQ=255)
                                ggTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcgc                                                                 >  2:5667123/1‑101 (MQ=255)
                                 gTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGcc                                                                <  2:5775645/101‑1 (MQ=255)
                                  ttCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCa                                                               >  1:2673788/1‑101 (MQ=255)
                                  ttCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCa                                                               >  1:4714616/1‑101 (MQ=255)
                                    cGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAgg                                                             >  1:2298233/1‑101 (MQ=255)
                                    cGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAgg                                                             <  1:6132793/101‑1 (MQ=255)
                                     gAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgc                                                            >  2:8214358/1‑101 (MQ=255)
                                     gAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgc                                                            <  2:261711/101‑1 (MQ=255)
                                      aGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgcc                                                           >  2:2155611/1‑101 (MQ=255)
                                      aGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgcc                                                           <  2:8293796/101‑1 (MQ=255)
                                      aGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgcc                                                           >  2:4560111/1‑101 (MQ=255)
                                       ggCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccg                                                          <  1:7756711/101‑1 (MQ=255)
                                         cgccgcGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgcc                                                        >  1:7400797/1‑101 (MQ=255)
                                          gccgcGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccg                                                       <  2:1935723/101‑1 (MQ=255)
                                          gccgcGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccg                                                       >  1:4861887/1‑101 (MQ=255)
                                            ttccgatctagaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgcc                                                     <  1:2778427/92‑1 (MQ=255)
                                            cgcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgcc                                                     <  2:9180978/101‑1 (MQ=255)
                                             gcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccg                                                    <  1:3442953/101‑1 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  <  1:1587773/101‑1 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  <  1:1741872/101‑1 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  >  1:8326221/1‑101 (MQ=255)
                                                   cGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgccc                                              >  1:5178802/1‑101 (MQ=255)
                                                    gagaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgcccg                                             >  1:8351226/1‑101 (MQ=255)
                                                     agaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgcccgc                                            <  1:3967426/101‑1 (MQ=255)
                                                        cGAAAAACACCCATGGCACCCGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGcc                                         <  1:5625182/101‑1 (MQ=255)
                                                         gAAAAACACCCATGGCCCCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                        <  2:3710034/101‑1 (MQ=255)
                                                         gAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                        >  2:6532424/1‑101 (MQ=255)
                                                         gAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                        >  2:3913469/1‑101 (MQ=255)
                                                           aaaccccccATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtat                                      <  2:289949/95‑1 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                     <  1:2652145/101‑1 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                     >  2:4423022/1‑101 (MQ=255)
                                                             aaCCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATAc                                    <  2:7603454/101‑1 (MQ=255)
                                                             aaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATAc                                    <  1:8541796/101‑1 (MQ=255)
                                                              acacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACg                                   <  1:8014674/101‑1 (MQ=255)
                                                              acacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACg                                   >  1:6763717/1‑101 (MQ=255)
                                                              acacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACg                                   >  1:6972181/1‑101 (MQ=255)
                                                              acacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACg                                   >  1:1194730/1‑101 (MQ=255)
                                                               cacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGc                                  <  1:10980/101‑1 (MQ=255)
                                                                   cATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcg                              >  2:3637671/1‑101 (MQ=255)
                                                                    aTGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgc                             >  2:3961186/1‑101 (MQ=255)
                                                                     tGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgcg                            <  1:1015264/101‑1 (MQ=255)
                                                                     tGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgcg                            >  1:2154224/1‑101 (MQ=255)
                                                                       gccccGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGcc                          <  2:7889621/98‑1 (MQ=255)
                                                                        cACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCa                         >  1:8693830/1‑101 (MQ=255)
                                                                        cACCGCCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGCCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCa                         <  2:7329200/101‑1 (MQ=255)
                                                                          ccGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATc                       <  2:5191308/101‑1 (MQ=255)
                                                                           cGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcg                      <  2:1641251/101‑1 (MQ=255)
                                                                               tGCCCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                  <  2:4685459/101‑1 (MQ=255)
                                                                               tGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                  <  1:4647539/101‑1 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 >  1:3634794/1‑101 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 >  1:5462192/1‑101 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 >  1:7225778/1‑101 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 >  2:3020228/1‑101 (MQ=255)
                                                                                 cACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCgg                >  1:5217976/1‑101 (MQ=255)
                                                                                    ccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCg             <  1:6293541/101‑1 (MQ=255)
                                                                                    ccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCg             >  1:8633066/1‑101 (MQ=255)
                                                                                    ccTGTCTTCGGCCGTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCg             <  2:6116102/101‑1 (MQ=255)
                                                                                      tGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAc           >  1:195284/1‑101 (MQ=255)
                                                                                       gTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAcc          >  2:4894990/1‑101 (MQ=255)
                                                                                       gTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAcc          <  1:2439618/101‑1 (MQ=255)
                                                                                        tCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCg         >  2:2224846/1‑101 (MQ=255)
                                                                                        tCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCg         >  2:7022940/1‑101 (MQ=255)
                                                                                         cTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCgg        >  1:9041515/1‑101 (MQ=255)
                                                                                         cTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCgg        <  2:5407362/101‑1 (MQ=255)
                                                                                          ttCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGc       >  2:1327526/1‑101 (MQ=255)
                                                                                           tCGGCCTTGGCGCCGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCt      >  1:8197623/1‑101 (MQ=255)
                                                                                            cGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCtt     >  1:2017365/1‑101 (MQ=255)
                                                                                            cGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCtt     <  2:8517003/101‑1 (MQ=255)
                                                                                              gCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtc   <  1:3157875/101‑1 (MQ=255)
                                                                                               ccTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtcg  >  1:5020384/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCG  >  NC_014034/3042855‑3043050

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG