Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,125 T→A 1.0% intergenic (‑108/+184) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1250T→A 1.0% 3.1 ‑2.0 ‑1.8398intergenic (‑108/+184)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (222/170):  total (228/170)
Fisher's exact test strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.48e-02

Only 100 of 438 total aligned reads displayed.

TCCTCCGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGC  >  NC_014034/3068025‑3068225
                                                                                                    |                                                                                                     
tcctccGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGt                                                                                                       >  2:1059160/1‑101 (MQ=255)
 cctccGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTg                                                                                                      <  2:8470718/101‑1 (MQ=255)
 cctccGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTg                                                                                                      <  1:8978280/101‑1 (MQ=255)
 cctccGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTg                                                                                                      >  2:6824450/1‑101 (MQ=255)
   tccgctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGc                                                                                                    <  2:7730630/101‑1 (MQ=255)
      gctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTc                                                                                                 <  2:7827456/101‑1 (MQ=255)
       ctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCg                                                                                                <  2:5494278/101‑1 (MQ=255)
        tccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGt                                                                                               >  2:7202444/1‑101 (MQ=255)
         ccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTg                                                                                              <  1:4799046/101‑1 (MQ=255)
         ccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGACGAGGTCATGCGGAACAGTGGCCTCGTg                                                                                              >  2:2250189/1‑101 (MQ=255)
          cgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGa                                                                                             >  2:379654/1‑101 (MQ=255)
           ggccAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            >  2:861434/4‑101 (MQ=255)
           ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            <  1:3400384/101‑1 (MQ=255)
           ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            <  2:5749219/101‑1 (MQ=255)
           ggTAAAAAGTTATGACCAATTTTGCGGATTCGCGCATCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            <  1:2250256/101‑1 (MQ=255)
            gTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAcc                                                                                           >  2:3256389/1‑101 (MQ=255)
             tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  1:733121/101‑1 (MQ=255)
             tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  1:1354384/101‑1 (MQ=255)
              cAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgc                                                                                         >  1:2807941/1‑101 (MQ=255)
              cAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgc                                                                                         <  1:8025475/101‑1 (MQ=255)
               aaaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgcc                                                                                        <  2:3183821/101‑1 (MQ=255)
                aaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgccg                                                                                       >  1:2599334/1‑101 (MQ=255)
                 aaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGa                                                                                      <  2:282352/101‑1 (MQ=255)
                  aGTTATGACCAATTTTGCGGATTCGCGCAGCCGAAACGATGGGCAGGCTGACCGAGGCTATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                     <  2:4366516/101‑1 (MQ=255)
                  aGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                     <  1:8718098/101‑1 (MQ=255)
                  aGTTATGACCAATTTTGCAGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                     <  2:2931912/101‑1 (MQ=255)
                   gTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAgg                                                                                    >  2:4003350/1‑101 (MQ=255)
                   gTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAgg                                                                                    >  1:1429682/1‑101 (MQ=255)
                    ttATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAggg                                                                                   >  1:4935392/1‑101 (MQ=255)
                    ttATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAggg                                                                                   >  2:5114902/1‑101 (MQ=255)
                     tATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGTGc                                                                                  >  1:3913993/1‑101 (MQ=255)
                      aTGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGcc                                                                                 >  1:5379339/1‑101 (MQ=255)
                         aCCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaa                                                                              <  2:5238109/101‑1 (MQ=255)
                          ccAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaaa                                                                             <  2:6648688/101‑1 (MQ=255)
                          ccAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaaa                                                                             >  1:70149/1‑101 (MQ=255)
                           cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                            <  2:1255365/101‑1 (MQ=255)
                           cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                            >  1:1169266/1‑101 (MQ=255)
                               tttGTGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGccc                                                                        >  1:3151221/1‑101 (MQ=255)
                               tttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGccc                                                                        >  1:4093074/1‑101 (MQ=255)
                                 tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                      <  1:616306/101‑1 (MQ=255)
                                 tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                      <  2:3917471/101‑1 (MQ=255)
                                  gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     <  2:4792288/101‑1 (MQ=255)
                                  gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     <  2:1726236/101‑1 (MQ=255)
                                  gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     >  1:1215879/1‑101 (MQ=255)
                                   cGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCgg                                                                    >  1:5101880/1‑101 (MQ=255)
                                     gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                  <  1:5421695/101‑1 (MQ=255)
                                      aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 <  2:7477744/101‑1 (MQ=255)
                                      aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 >  2:7589211/1‑101 (MQ=255)
                                      aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 >  2:8784093/1‑101 (MQ=255)
                                        tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgc                                                               >  2:5681178/1‑101 (MQ=255)
                                            gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           <  1:4551721/101‑1 (MQ=255)
                                            gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           <  1:2969092/101‑1 (MQ=255)
                                            gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           <  1:9039536/101‑1 (MQ=255)
                                               aCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgcc                                                        >  1:3309413/1‑101 (MQ=255)
                                                ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCgcccgccc                                                       <  1:7469519/101‑1 (MQ=255)
                                                  gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCGAGCGcccggcccg                                                     >  2:4143148/1‑101 (MQ=255)
                                                  gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                     <  1:379662/101‑1 (MQ=255)
                                                  gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                     >  2:4586169/1‑101 (MQ=255)
                                                    gacaaggggCAGGCTGACCGAGGCCATCGCAAAGGTCATGCGGAACACTCGCCTCGTGACCGCCGAGGGCCCAAAGGGCCTCGGCCCGCGAccgccccgcc                                                   >  2:2465757/7‑101 (MQ=17)
                                                     aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgccc                                                  >  1:3488106/1‑101 (MQ=255)
                                                      cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                 <  1:5822898/101‑1 (MQ=255)
                                                        aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                               <  1:323689/101‑1 (MQ=255)
                                                         tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                              >  1:2093498/1‑101 (MQ=255)
                                                         tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                              <  2:1305257/101‑1 (MQ=255)
                                                          gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                             >  1:7600599/1‑101 (MQ=255)
                                                          gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                             <  1:6075451/101‑1 (MQ=255)
                                                          gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                             >  1:8148026/1‑101 (MQ=255)
                                                          gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCACggc                                             >  1:1794428/1‑101 (MQ=255)
                                                           ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcg                                            <  2:7129024/101‑1 (MQ=255)
                                                              aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggg                                         >  1:8277500/1‑100 (MQ=255)
                                                               ggCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggcg                                        >  1:7278879/1‑101 (MQ=255)
                                                                 cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.t                                     <  2:8360077/101‑1 (MQ=255)
                                                                    aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.Ttct                                  <  1:7730494/101‑1 (MQ=255)
                                                                       gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgc                               <  1:6856236/101‑1 (MQ=255)
                                                                        aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgcc                              >  2:3057165/1‑101 (MQ=255)
                                                                        aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgcc                              <  1:3621937/101‑1 (MQ=255)
                                                                            cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgcccgcc                          >  2:2027852/1‑101 (MQ=255)
                                                                            cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgcccccc                          >  1:5711429/1‑101 (MQ=255)
                                                                            cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.CTCTcgcccccc                          >  2:6085913/1‑101 (MQ=255)
                                                                             aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTcgcccgccc                         >  1:6355244/1‑100 (MQ=255)
                                                                                gCCGAGGCCAATCGCAACAGAGGTCCTGTGACGGCCGAGGGCCCAAAGGCACTCGGCCTGCGCCCCCCCCGCCCTCGGCGGGCGC.TGTGCACccgccgccg                      >  2:7021701/1‑98 (MQ=21)
                                                                                  cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGgggcg                    >  2:6378314/1‑101 (MQ=255)
                                                                                   gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGgggcgg                   >  1:8515396/1‑101 (MQ=255)
                                                                                      gTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCGCTCGCGCCCGCCCggccgggg                 >  2:3696787/1‑100 (MQ=18)
                                                                                      gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGgggcggggg                >  1:6495841/1‑99 (MQ=255)
                                                                                       tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCCGGGCGGgcgc               >  2:8128144/1‑101 (MQ=255)
                                                                                        cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGcc              <  2:5849200/101‑1 (MQ=255)
                                                                                         aTGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCg             <  1:2397817/101‑1 (MQ=255)
                                                                                          tGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCCGGGCGGGCGcccg            >  2:3496210/1‑99 (MQ=255)
                                                                                           gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCGCGCCGGGGCGGGGGCCGGc           >  2:1632384/1‑101 (MQ=255)
                                                                                            cGGAACAGTGGCCTCGTGACGGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGcc          <  1:7202748/101‑1 (MQ=255)
                                                                                              gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCctc        <  1:1569293/101‑1 (MQ=255)
                                                                                              gAACAGTGCCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCctc        <  2:7304891/101‑1 (MQ=255)
                                                                                              gAACAGTGCCCTCGGGCCCGCCGAGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCctc        <  1:6748695/101‑1 (MQ=14)
                                                                                               gagggTGGCCTGGGGCCCGCCGGGGGCCCAAAGGCCGTGGGCCAGCCCCCGCCCCGCCCATGGCGGCCGC.TTCTCGCCCGCCGGGGGGGGCGCCGGCctct       <  2:1429650/97‑1 (MQ=11)
                                                                                               aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCCGCctcc       >  1:7872490/1‑100 (MQ=255)
                                                                                                accgTGGCCTCGGGCCCGCCGAGGGCCCAAAGCCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctg      <  2:8916513/98‑1 (MQ=14)
                                                                                                aCAGGGGCCTCGTGCCCGCCGAGGGCCCAAGGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctg      <  2:5407538/101‑1 (MQ=14)
                                                                                                 cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGcccccgc     >  1:1921771/1‑96 (MQ=255)
                                                                                                    tGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc  <  1:1059178/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCCTCCGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGC  >  NC_014034/3068025‑3068225

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG