Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,128 C→T 1.5% intergenic (‑111/+181) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1280C→T 1.5% 5.3 ‑3.2 ‑0.7387intergenic (‑111/+181)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (218/163):  total (224/163)
Fisher's exact test strand distribution p-value = 4.18e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.85e-01

Only 100 of 428 total aligned reads displayed.

TCCGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCG  >  NC_014034/3068028‑3068226
                                                                                                    |                                                                                                  
tccgctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGc                                                                                                    <  1:1472044/101‑1 (MQ=255)
tccgctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGc                                                                                                    <  2:7937227/101‑1 (MQ=255)
  ctctccgGTCAAAAGTTATGACCAATTTGGCGGATCCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATTGCCGAGGTCATGCGGAACAGTGGCCt                                                                                                  <  2:126808/99‑1 (MQ=255)
   gctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTc                                                                                                 <  2:1338861/101‑1 (MQ=255)
     tccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGt                                                                                               <  1:4825520/101‑1 (MQ=255)
     tccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGt                                                                                               >  2:7202444/1‑101 (MQ=255)
      ccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTg                                                                                              >  1:2351039/1‑101 (MQ=255)
      ccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTg                                                                                              >  2:3806020/1‑101 (MQ=255)
       cgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGa                                                                                             >  1:3050960/1‑101 (MQ=255)
        ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            <  2:5749219/101‑1 (MQ=255)
        ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                            <  2:4183047/101‑1 (MQ=255)
          tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  1:733121/101‑1 (MQ=255)
          tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  1:1354384/101‑1 (MQ=255)
          tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  2:5379497/101‑1 (MQ=255)
          tCAAAAGTTATGACCAATGTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccg                                                                                          <  2:3090749/101‑1 (MQ=255)
            aaaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgcc                                                                                        <  1:3273675/101‑1 (MQ=255)
            aaaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgcc                                                                                        >  2:5623251/1‑101 (MQ=255)
            aaaaGTTATGACCAATTTTGCGGATTCGCGCAAACGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgcc                                                                                        >  1:6459475/1‑101 (MQ=255)
             aaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgccg                                                                                       >  1:5842202/1‑101 (MQ=255)
             aaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGGGGCCTCGTGAccgccg                                                                                       >  2:2973938/1‑101 (MQ=255)
               aGTTATGACCAATTTTGCGGATTCGCGCAGCCGAAACGATGGGCAGGCTGACCGAGGCTATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                     <  2:4366516/101‑1 (MQ=255)
               aGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                     <  1:8718098/101‑1 (MQ=255)
                gTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAgg                                                                                    >  2:4003350/1‑101 (MQ=255)
                 ttATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAggg                                                                                   >  2:5114902/1‑101 (MQ=255)
                  tATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGc                                                                                  >  2:7750792/1‑101 (MQ=255)
                   aTGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCAATCCCCGAGGCCATGCGGAACAGTGGCCTCTTGAcggcaagcgggc                                                                                 >  2:8734228/1‑93 (MQ=255)
                      aCCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaa                                                                              >  1:4741153/1‑101 (MQ=255)
                      aCCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaa                                                                              <  2:5238109/101‑1 (MQ=255)
                       ccAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaaa                                                                             <  2:6648688/101‑1 (MQ=255)
                       ccAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaaa                                                                             >  1:70149/1‑101 (MQ=255)
                        cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                            >  1:1169266/1‑101 (MQ=255)
                         aaTTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAgg                                                                           <  1:3368467/101‑1 (MQ=255)
                         aaTTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAgg                                                                           <  2:2301055/101‑1 (MQ=255)
                          aTTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGc                                                                          >  1:8465190/1‑101 (MQ=255)
                              tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                      >  1:4442011/1‑101 (MQ=255)
                              tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                      <  2:3917471/101‑1 (MQ=255)
                               gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     <  2:4792288/101‑1 (MQ=255)
                               gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     >  2:6863405/1‑101 (MQ=255)
                               gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                     >  1:1215879/1‑101 (MQ=255)
                                  gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                  <  2:8163943/101‑1 (MQ=255)
                                   aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 >  1:3837130/1‑101 (MQ=255)
                                   aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 >  1:3592615/1‑101 (MQ=255)
                                   aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 <  2:7477744/101‑1 (MQ=255)
                                   aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 >  2:7589211/1‑101 (MQ=255)
                                   aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                 <  2:1481124/101‑1 (MQ=255)
                                    ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAg                                                                <  1:406083/101‑1 (MQ=255)
                                    ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAg                                                                <  2:3227912/101‑1 (MQ=255)
                                     tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgc                                                               >  2:5681178/1‑101 (MQ=255)
                                      cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcg                                                              >  2:2085416/1‑101 (MQ=255)
                                         gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           <  1:9039536/101‑1 (MQ=255)
                                         gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           <  2:744319/101‑1 (MQ=255)
                                         gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                           >  2:8902087/1‑101 (MQ=255)
                                          cAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccg                                                          <  1:5168041/101‑1 (MQ=255)
                                           aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                         >  2:3299338/1‑101 (MQ=255)
                                           aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                         <  1:6909275/101‑1 (MQ=255)
                                             ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCgcccgccc                                                       <  1:7469519/101‑1 (MQ=255)
                                              cGAAACGATGGGCAGGCTGACCGAGCCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGCCCCTCGGCCAGCgcccgcccg                                                      >  1:1634857/1‑100 (MQ=255)
                                               gAACCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                     <  2:2861798/101‑1 (MQ=255)
                                               gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                     >  2:4586169/1‑101 (MQ=255)
                                               gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                     >  1:7301144/1‑101 (MQ=255)
                                                  aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgccc                                                  >  1:3488106/1‑101 (MQ=255)
                                                    gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                <  1:764135/101‑1 (MQ=255)
                                                     aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                               >  2:1119546/1‑101 (MQ=255)
                                                     aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                               >  2:1733316/1‑101 (MQ=255)
                                                     aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                               <  1:6575952/101‑1 (MQ=255)
                                                      tGGGCAGCCTGACCGAGGCCATCGCCGAGGCCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                              <  1:2940178/101‑1 (MQ=255)
                                                       gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                             >  1:8148026/1‑101 (MQ=255)
                                                       gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCACggc                                             >  1:1794428/1‑101 (MQ=255)
                                                        ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcg                                            <  2:7129024/101‑1 (MQ=255)
                                                           aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggg                                         >  1:8277500/1‑100 (MQ=255)
                                                           aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggg                                         >  2:7042343/1‑100 (MQ=255)
                                                           aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                         >  2:2759477/1‑101 (MQ=255)
                                                               tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                     >  1:5389126/1‑101 (MQ=255)
                                                                 aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                   <  1:2238565/101‑1 (MQ=255)
                                                                 aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                   <  1:7730494/101‑1 (MQ=255)
                                                                  ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctc                                  >  1:2783319/1‑101 (MQ=255)
                                                                    gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctccc                                >  1:4938790/1‑99 (MQ=255)
                                                                    gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgc                                >  2:8592585/1‑101 (MQ=255)
                                                                      ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgccc                              <  1:8013050/101‑1 (MQ=255)
                                                                       gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccc                             >  1:6107288/1‑100 (MQ=255)
                                                                         cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTcgcccccc                           >  2:6085913/1‑101 (MQ=255)
                                                                           tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgg                         <  2:8397469/101‑1 (MQ=255)
                                                                            cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCcgg                        >  2:3699559/1‑101 (MQ=255)
                                                                             gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCGCCCCCCgggg                       >  1:3787202/1‑101 (MQ=255)
                                                                              ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCCCCCGCCcgggg                      >  1:2588509/1‑100 (MQ=255)
                                                                               cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcg                     >  2:6378314/1‑101 (MQ=255)
                                                                                   gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGggcgggcg                 >  1:5727189/1‑101 (MQ=255)
                                                                                     cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGcc               <  2:5849200/101‑1 (MQ=255)
                                                                                     cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGcc               >  1:619459/1‑101 (MQ=255)
                                                                                      aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCg              <  1:5505489/101‑1 (MQ=255)
                                                                                       tGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGCGGGCGcccg             >  2:3496210/1‑99 (MQ=255)
                                                                                        gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCGCGCCGGGGCGGGGGCCGGc            >  2:1632384/1‑101 (MQ=255)
                                                                                         cGGAACAGTGGCCTCGTGACGGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGcc           <  1:7202748/101‑1 (MQ=255)
                                                                                            aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCCGCctcc        >  1:7872490/1‑100 (MQ=255)
                                                                                              cggTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc      <  1:8916399/99‑1 (MQ=255)
                                                                                              cggTGGCCTCGGGCCCGCCGGGGGCCAAAAGCCCCTCGCCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc      <  1:6786758/99‑1 (MQ=14)
                                                                                              cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGcccccgc      >  1:1921771/1‑96 (MQ=255)
                                                                                              cAGTGCCCTCGGGCCCGCCGGGGCCCCAAAGCCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc      <  1:3215453/101‑1 (MQ=11)
                                                                                                 tGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc   <  1:1059178/101‑1 (MQ=255)
                                                                                                  ggCCTCGGGCCCGCCGAGGCCCCAAGGCCCCGCGCCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg  <  1:2027913/101‑1 (MQ=14)
                                                                                                    |                                                                                                  
TCCGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCG  >  NC_014034/3068028‑3068226

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG