Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,155 C→A 1.7% intergenic (‑138/+154) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1550C→A 1.7% 4.7 ‑2.3 ‑0.0339intergenic (‑138/+154)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (215/118):  total (221/118)
Fisher's exact test strand distribution p-value = 9.58e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.60e-01

Only 100 of 443 total aligned reads displayed.

TTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGG  >  NC_014034/3068055‑3068255
                                                                                                     |                                                                                                        
  ttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCt                                                                                                        <  2:1301031/101‑1 (MQ=255)
  ttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCt                                                                                                        >  1:1954880/1‑101 (MQ=255)
   tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTc                                                                                                       >  2:317280/1‑101 (MQ=255)
   tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTc                                                                                                       <  1:616306/101‑1 (MQ=255)
   tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTc                                                                                                       <  2:6950478/101‑1 (MQ=255)
   tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTc                                                                                                       >  2:224771/1‑101 (MQ=255)
    gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCg                                                                                                      >  1:1215879/1‑101 (MQ=255)
    gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCg                                                                                                      <  1:1171082/101‑1 (MQ=255)
       gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGcc                                                                                                   <  2:1824646/101‑1 (MQ=255)
       gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGcc                                                                                                   <  1:7669621/101‑1 (MQ=255)
       gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCCAGGGCCCAAAGG.CCCTCGGcc                                                                                                   >  2:4260575/1‑101 (MQ=255)
        aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.a                                                                                                 >  2:7469195/1‑101 (MQ=255)
        aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.a                                                                                                 >  2:8784093/1‑101 (MQ=255)
          tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.Agc                                                                                               <  2:3358585/101‑1 (MQ=255)
               cAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGcgcccg                                                                                          >  1:5658973/1‑101 (MQ=255)
                aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGcgcccgc                                                                                         >  2:3299338/1‑101 (MQ=255)
                aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGcgcccgc                                                                                         >  1:7326377/1‑101 (MQ=255)
                aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGcgcccgc                                                                                         >  2:5153577/1‑101 (MQ=255)
                 aCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGcgcccgcc                                                                                        >  1:3309413/1‑101 (MQ=255)
                  ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCgcccgccc                                                                                       >  1:5407723/1‑101 (MQ=255)
                  ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCgcccgccc                                                                                       <  2:7739996/101‑1 (MQ=255)
                   cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGcccgcccc                                                                                      <  2:1722011/101‑1 (MQ=255)
                    gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGcccgccccg                                                                                     <  1:379662/101‑1 (MQ=255)
                    gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGcccgccccc                                                                                     >  2:7170510/1‑100 (MQ=255)
                      aaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCccgccccgcc                                                                                   <  1:8045559/101‑1 (MQ=255)
                        cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCa                                                                                 >  1:2632500/1‑101 (MQ=255)
                         gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCAt                                                                                <  2:2807842/101‑1 (MQ=255)
                          aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATg                                                                               >  2:1426861/1‑101 (MQ=255)
                           tGGGCGGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCG.AGCGCCCGCCCCGCCCACgg                                                                              >  1:7237708/1‑101 (MQ=255)
                            gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATggc                                                                             >  1:8148026/1‑101 (MQ=255)
                            gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATggc                                                                             <  1:6075451/101‑1 (MQ=255)
                               cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATggc.ggg                                                                         <  2:4550474/101‑1 (MQ=255)
                                aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATggc.gggg                                                                        >  1:8739067/1‑100 (MQ=255)
                                     gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.Ttc                                                                  <  1:5218211/101‑1 (MQ=255)
                                      aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.Ttct                                                                 <  2:3578909/101‑1 (MQ=255)
                                       ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.Ttctc                                                                >  1:2783319/1‑101 (MQ=255)
                                         gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.c                                                             >  2:3966221/1‑101 (MQ=255)
                                          aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.cc                                                            <  1:3621937/101‑1 (MQ=255)
                                           ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.ccc                                                           <  2:4845595/101‑1 (MQ=255)
                                           ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.ccc                                                           <  1:7780299/101‑1 (MQ=255)
                                           ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.CTCTcg.ccc                                                           >  1:3464003/1‑101 (MQ=255)
                                            gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.cccg                                                          >  2:5638020/1‑101 (MQ=255)
                                              cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.cccgcc                                                        >  2:2027852/1‑101 (MQ=255)
                                              cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcg.cccgcc                                                        >  1:4779698/1‑101 (MQ=255)
                                               aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.Cccgccg                                                       >  2:57731/1‑101 (MQ=255)
                                                tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCgg                                                      <  2:8397469/101‑1 (MQ=255)
                                                     gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGgggcgg                                                 >  1:2127396/1‑101 (MQ=255)
                                                       ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGgggcgggg                                               >  2:379554/1‑100 (MQ=255)
                                                        gTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCC.TGCGCCCGCCCCGCCATCGGC.GGGGGCGCTCGCG.CCCGCCCggccgggg                                               >  2:3696787/1‑100 (MQ=18)
                                                           aTGCGGGACAGGGTTCCCGCGACCGCCGAGTGCCCAAA.G.CACTCGGCC.AGCGCCCCCCCCGCCCATGGC.GGGCGA.TTCTCG.CCCCCCGGGGCCGGGGCCgg                                          >  1:1732443/1‑101 (MQ=17)
                                                           aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCg                                           <  1:6824721/101‑1 (MQ=255)
                                                           aTGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCg                                           <  1:2397817/101‑1 (MQ=255)
                                                              cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCgggc                                        >  1:7878682/1‑99 (MQ=255)
                                                                gAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCctc                                      <  1:7049398/101‑1 (MQ=255)
                                                                gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCGCAGGGC.GGGCGA.GGCACC.CCAGCCGCGGCCGGCGCGGGcccc                                      >  1:2918726/1‑99 (MQ=14)
                                                                gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGGGC.TTCTCG.CCCCCCCGGGCGGGCCCCCGccct                                      >  2:6520470/1‑99 (MQ=17)
                                                                  actgTGCCCTCGTGCCCGCCGAGGCCCAAAGGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTctg                                    <  2:7946725/98‑1 (MQ=14)
                                                                  accgTGGCCTCGGGCCCGCCGAGGGCCCAAAGC.CCCTCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTctg                                    <  2:8916513/98‑1 (MQ=14)
                                                                  aCAGGGGCCTCGTGCCCGCCGAGGGCCCAAGGG.CCCTCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTctg                                    <  2:5407538/101‑1 (MQ=14)
                                                                   cAGTGCCCTCGGGCCCGCCGGGGCCCCAAAGC.CCCTCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTctgc                                   <  1:3215453/101‑1 (MQ=11)
                                                                    agaagtcccGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCC.TGCGCCCGCCCCGCCATCGGC.GGGCGCGTTCCCG.CCCCCCGGGGTGCGGGCCGGGCCctgc                                   >  2:7411924/9‑101 (MQ=14)
                                                                    aatGGCCCCGTGACCGGCGAGGGCCCAAAGG.CCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGC.GTCCCG.CCGCCCGGGGCGGGCGGCggacccccg                                   >  2:1615408/3‑94 (MQ=14)
                                                                    aGTGCCCTGGTCCCCGCGGAGGGCCCAAAGG.CCCTCGGCG.GGCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTctgct                                  <  2:7080924/101‑1 (MQ=14)
                                                                      tGGCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                <  1:1059178/101‑1 (MQ=255)
                                                                       ggCCTCGGGCCCGCCGAGGCCCCAAGGC.CCCGCGCCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                               <  1:2027913/101‑1 (MQ=14)
                                                                           tCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCTg                           >  1:3864218/1‑101 (MQ=255)
                                                                           ccgggcccgccgAGGGCCCAAAGG.CCCTCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTg                           <  1:7539127/95‑1 (MQ=255)
                                                                            cgggcccgccgGGGGCCCAAAGC.CCCGCGCCC.GGCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgg                          <  1:3123682/96‑1 (MQ=11)
                                                                            cGTGACCGCCGGGGGCCCAAGGG.CCCCCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgg                          <  2:3874410/101‑1 (MQ=14)
                                                                            cGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCC.TGCGCCCGCCCCGCCATCGGC.GGGCGCGGTTGCC.CCCCCGGGGGCGGGCGGGGGGCGCTGCCGCGCCTg                           >  2:8311405/1‑101 (MQ=2)
                                                                            cGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTgg                          >  1:355807/1‑101 (MQ=255)
                                                                              tgccagccgGGGGCCCAAAGG.CCGCCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGGGC.TTCTCGCCCCCCCCGGCCGGGCGCCGGCCGC.GGTGCGCCttcgg                        >  2:3023185/6‑97 (MQ=14)
                                                                                 ccgccgGGGCCCCAAGGG.CCCCCGCCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                     <  1:2974036/101‑1 (MQ=14)
                                                                                  cgccgA.GGCCCAAAGGCCCCCCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAAc                    <  2:2599252/101‑1 (MQ=14)
                                                                                   gccgAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGGAACg                   >  1:8281264/1‑101 (MQ=255)
                                                                                    ccgGGGGCCCCAAGGCCCCCCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                   <  1:7595239/101‑1 (MQ=14)
                                                                                    cccggggCCAAAAGG.CCCCCGGCCGGGGGCCCGCCCCGCCCATGCC.GGGCGC.TTCTCC.CCCGCCGGGGCGGCCGCCGGCCTCTGCTGCGCCTGGGGAAACg                   <  1:412962/97‑1 (MQ=14)
                                                                                     cgAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGGGCCCGCCTCTGCCGCGCCTGGGGGAACggg                 >  2:3065889/1‑100 (MQ=17)
                                                                                      gggggCCCAAGGC.CCCTCGCCC.AGCCNCNGNCCCGCCCAGGGC.GGGCGC.TTCTCG.CCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTc                <  1:5863725/99‑1 (MQ=17)
                                                                                      gAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGGAACGGTc                >  1:6648065/1‑101 (MQ=255)
                                                                                       ggggGCCAAAGGCCCCTCGCCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTc                <  2:7581682/100‑1 (MQ=17)
                                                                                        gggCCAAAGGG.CCCCCGGCC.AGCGCCCCCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt              <  1:509586/101‑1 (MQ=17)
                                                                                         ggCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGAGACGGGCTTg             >  2:6055662/1‑101 (MQ=255)
                                                                                         ggCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGGGCCGGCCTCTGCCGCGCCTGGGGGAACGGGCTTg             >  1:1839066/1‑101 (MQ=17)
                                                                                          gCCAAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTgg            >  1:8803831/1‑101 (MQ=255)
                                                                                           cccAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGG.GGGCGC.TTCTCG.CCCGCCGGGGCGAGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc           >  1:6573064/1‑101 (MQ=255)
                                                                                             cAAGGG.CCCCCGCCC.GGCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAc         <  1:1617151/101‑1 (MQ=18)
                                                                                              caaggccccTCGGCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAc         <  2:6173878/95‑1 (MQ=255)
                                                                                              aaaGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCCGCCCCCGCCGCGCCTGGGGAAACGGGCTTGGCAcc        >  1:396260/1‑101 (MQ=17)
                                                                                               aggg.CCCTGGGCC.GGCGCCCGCCCCGCCCAGGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGACACCg       <  1:6320279/99‑1 (MQ=18)
                                                                                               aaggccccGCGGCC.AGCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc        <  2:681902/96‑1 (MQ=255)
                                                                                               aaGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTTCTGCGCCTGGGGGAACGGGCTTGGGACCg       >  2:5306511/1‑101 (MQ=17)
                                                                                               aaGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAccc       >  1:9089204/1‑100 (MQ=255)
                                                                                                aGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGc      >  2:1114736/1‑101 (MQ=255)
                                                                                                 gg.CCCTGGCCC.AGCGCCCGCCCCGCCCATGGG.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc     <  1:1409951/101‑1 (MQ=255)
                                                                                                 gg.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc     >  2:5952175/1‑101 (MQ=255)
                                                                                                 gg.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCCGCTGCGCCCGGGGGAACGGTCTTGGCACCGcc     >  1:8508470/1‑101 (MQ=255)
                                                                                                  g.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCCGCGCCTGGGGGAACCGTCTTGGCCCCCccc    >  1:8956468/1‑101 (MQ=17)
                                                                                                   ccccCCGCCC.GGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc    <  1:4429066/100‑1 (MQ=255)
                                                                                                    cccTCGCCC.GGCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg   <  1:6924062/101‑1 (MQ=255)
                                                                                                     |                                                                                                        
TTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCC.AGCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCG.CCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGG  >  NC_014034/3068055‑3068255

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG