Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,163 A→T 2.4% intergenic (‑146/+146) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1630A→T 2.4% 9.4 ‑2.3 ‑0.2296intergenic (‑146/+146)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (193/95):  total (200/96)
Fisher's exact test strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.84e-01

Only 100 of 416 total aligned reads displayed.

ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263
                                                                                                     |                                                                                                         
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCa                                                                                                           >  2:7469195/1‑101 (MQ=255)
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCa                                                                                                           >  2:7589211/1‑101 (MQ=255)
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCa                                                                                                           >  2:8784093/1‑101 (MQ=255)
 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTGGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAg                                                                                                          <  1:6471193/101‑1 (MQ=255)
 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAg                                                                                                          <  2:6154395/101‑1 (MQ=255)
 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAg                                                                                                          <  1:1202424/101‑1 (MQ=255)
 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAg                                                                                                          <  1:1976826/101‑1 (MQ=255)
  tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAgc                                                                                                         <  2:3358585/101‑1 (MQ=255)
   cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAgcg                                                                                                        >  2:2085416/1‑101 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGcgccc                                                                                                     <  1:4551721/101‑1 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGcgccc                                                                                                     <  1:2969092/101‑1 (MQ=255)
       cAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGcgcccg                                                                                                    >  1:3579017/1‑101 (MQ=255)
          ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCgcccgccc                                                                                                 >  1:5407723/1‑101 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGcccgccccg                                                                                               <  1:379662/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGcccgccccg                                                                                               <  1:3264862/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGcccgccccg                                                                                               >  1:7301144/1‑101 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCAt                                                                                         <  2:2807842/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATg                                                                                        >  1:1372665/1‑101 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATg                                                                                        >  2:1733316/1‑101 (MQ=255)
                   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATgg                                                                                       >  1:2093498/1‑101 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggc                                                                                      >  1:8148026/1‑101 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCACggc                                                                                      >  1:1794428/1‑101 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGA.GGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCCTggcg                                                                                     >  2:7228203/1‑101 (MQ=255)
                     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggcg                                                                                     <  1:6625240/101‑1 (MQ=255)
                     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggcg                                                                                     <  2:7129024/101‑1 (MQ=255)
                       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggcggg                                                                                   <  2:4550474/101‑1 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggcgggg                                                                                  >  1:8739067/1‑100 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATggcgggc                                                                                  >  2:6008639/1‑101 (MQ=255)
                          gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGgggc                                                                                >  1:6298955/1‑101 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ctc                                                                            >  1:3454767/1‑101 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttct                                                                           <  1:2238565/101‑1 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttct                                                                           <  2:3578909/101‑1 (MQ=255)
                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttctc                                                                          >  1:2298119/1‑101 (MQ=255)
                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttctc                                                                          <  1:3014571/101‑1 (MQ=255)
                               ccGAGGCCATCGCCGAGGGCATGCGGAACAGTGGCCCCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttctc                                                                          <  1:7589535/101‑1 (MQ=255)
                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttctccc                                                                        >  1:4938790/1‑99 (MQ=255)
                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.Ttctccc                                                                        >  1:6133416/1‑99 (MQ=255)
                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.c                                                                       <  2:2444130/101‑1 (MQ=255)
                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.cc                                                                      <  1:3178374/101‑1 (MQ=255)
                                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.ccc                                                                     <  1:8013050/101‑1 (MQ=255)
                                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.cccg                                                                    >  2:5638020/1‑101 (MQ=255)
                                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.cccg.cc                                                                 <  1:6914692/101‑1 (MQ=255)
                                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTcg.cccc.cc                                                                 >  1:5711429/1‑101 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.Cccg.ccg                                                                >  2:57731/1‑101 (MQ=255)
                                        tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCgg                                                               <  2:8397469/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGGCCGCCCCG.CCCATGGCGGGGGC.TTCTTG.GCCG.CCGgggcg                                                           >  2:4211547/1‑101 (MQ=18)
                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGgggcgg                                                          >  1:8515396/1‑101 (MQ=255)
                                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGgggcgggg                                                        >  2:379554/1‑100 (MQ=255)
                                                gTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCG.CCATCGGCGGGGGCGCTCGCG.CCCG.CCCggccgggg                                                        >  2:3696787/1‑100 (MQ=18)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGGGC.CTCTCG.CCCG.CCCGggcgggcggc                                                     >  2:6371486/1‑99 (MQ=255)
                                                      cGGAACAGTGGCCTCGTGACGGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGc.c                                                <  1:7202748/101‑1 (MQ=255)
                                                       ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGc.cc                                               >  1:1696942/1‑100 (MQ=255)
                                                        gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.ctc                                              <  1:1569293/101‑1 (MQ=255)
                                                         gagggTGGCCTGGGGCCCGCCGGGGGCCCAAAGG.CCGTGGGCCAGCCCCCGCCCCG.CCCATGGCGGCCGC.TTCTCG.CCCG.CCGGGGGGGGCGCCGGC.ctct                                             <  2:1429650/97‑1 (MQ=11)
                                                         aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCCGC.ctcc                                             >  1:7872490/1‑100 (MQ=255)
                                                          accgTGGCCTCGGGCCCGCCGAGGGCCCAAAGC.CCCTCGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTctg                                            <  2:8916513/98‑1 (MQ=14)
                                                          aCAGTGGTCCCATGACCCCCGAGTGCCCCAAGG.CACTCGGGCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCC.CCCGCCCGGGGAGGGGGCCGGc.cccc                                             >  1:5948/1‑98 (MQ=17)
                                                          aCAGGGGCCTCGTGCCCGCCGAGGGCCCAAGGG.CCCTCGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTctg                                            <  2:5407538/101‑1 (MQ=14)
                                                           cggTGGCCTCGGGCCCGCCGGGGGCCAAAAGC.CCCTCGCCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTctgc                                           <  1:6786758/99‑1 (MQ=14)
                                                            agaagtcccGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCG.CCATCGGCGGGCGCGTTCCCG.CCCC.CCGGGGTGCGGGCCGGG.CCctgc                                           >  2:7411924/9‑101 (MQ=14)
                                                            aGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCCCCCGCCCCG.CCCATGGCGGGCGC.CTCACT.CCCG.CCGGGGCCGGCGCCGGC.cacttcg                                          >  2:12736/1‑98 (MQ=14)
                                                              tGGCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCtgctgc                                        <  1:1059178/101‑1 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCtgctgc                                        >  1:7459814/1‑101 (MQ=255)
                                                                  cccGTGACCGCCGAGGGCCCCAAGG.CACTCGCCCAGCGCCCGCCCCG.CCCATGGCGGGCCCTTTCGCC.GCCC.CCGGGGCGGAC.CCGGCGCTctgccctgcc                                     >  2:3248003/3‑95 (MQ=17)
                                                                   tCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CCCTGCTGCGCCTg                                   >  1:3864218/1‑101 (MQ=255)
                                                                   cccggcccgcggGGGGCCCAACGG.CCCCCGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTg                                   <  1:4003465/95‑1 (MQ=11)
                                                                    cGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCG.CCATCGGCGGGCGCGGTTGCC.CCCC.CGGGGGCGGGCGGGGGG.CGCTGCCGCGCCTg                                   >  2:8311405/1‑101 (MQ=2)
                                                                     gggcccgccgGGGGCCCAAAGG.CCCGCGGCCCGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTggg                                 <  1:5153746/97‑1 (MQ=14)
                                                                     gTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTggg                                 <  2:3057337/101‑1 (MQ=255)
                                                                      tgccagccgGGGGCCCAAAGG.CCGCCGGCCAGCGCCCGCCCCG.CCCATGGCGGGGGC.TTCTCGCCCCC.CCCGGCCGGGCGCCGGC.CGC.GGTGCGCCttcgg                                >  2:3023185/6‑97 (MQ=14)
                                                                       gACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGGGCCCGC.CCCTGCCGCGCCCggggg                               >  1:7653507/1‑100 (MQ=14)
                                                                        aCCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCCggggga                              >  2:3839797/1‑99 (MQ=255)
                                                                         ccgccgAGGCCCAAAGGG.CCCTCGCCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGaaa                             <  2:1966920/101‑1 (MQ=17)
                                                                          cgccgA.GGCCCAAAGGCCCCCCGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAAc                            <  2:2599252/101‑1 (MQ=14)
                                                                           gccgGGGGCCCAAAGC.CCCGCGGCCGGCCCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACg                           <  1:8280545/101‑1 (MQ=14)
                                                                              gggggCCCAAAGG.CCCCCGGCCAGCGCCCGCCCCGCCCCATGGCGGGCGCGTTCGCG.CCGC.CCGGGGGTGGCGGCGGC.CCCTGTCGCGCCCggggccggtc                          >  1:7744116/3‑95 (MQ=18)
                                                                                gggCCGAAAGG.CACACGGGCGGCGCCCGCCCCG.CCAATGGCGGGCGG.TGCGCC.CCCG.CCGGGGGGGCCGGCGGC.CTCTGCTGCGCCTGGGGAAACGGTCtt                      <  2:1634810/101‑1 (MQ=9)
                                                                                gggCCAAAGGG.CCCGCGCCCAGCGCCCGCCCCG.CCCAGGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCtt                      <  1:7016360/101‑1 (MQ=17)
                                                                                  gCCCAAGGC.CCCTGGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTgg                    <  2:7008474/101‑1 (MQ=18)
                                                                                  gCCCAAGGC.CCCCCGCCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAACCGGTCTTgg                    <  1:8803034/101‑1 (MQ=17)
                                                                                   cccAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGGGGGCGC.TTCTCG.CCCG.CCGGGGCGAGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGc                   >  1:6573064/1‑101 (MQ=255)
                                                                                    ccAAGGG.CCCTCGCCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                  <  2:7794016/101‑1 (MQ=255)
                                                                                    ccAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGGCTTGGcc                  >  1:4141436/1‑100 (MQ=255)
                                                                                    ccAAAGC.CCCCCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                  <  2:4628395/101‑1 (MQ=255)
                                                                                       aaGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTTCTGCGCCTGGGGGAACGGGCTTGGGACCg               >  2:5306511/1‑101 (MQ=17)
                                                                                       aaGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg               >  2:6716883/1‑101 (MQ=255)
                                                                                        aggccccCCGGCCAGCCCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg               <  2:3151122/97‑1 (MQ=255)
                                                                                        aGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGc              >  2:1114736/1‑101 (MQ=255)
                                                                                          g.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCCGCGCCTGGGGGAACCGTCTTGGCCCCCccc            >  1:8956468/1‑101 (MQ=17)
                                                                                          g.CCCCCGCCCGGCGCCCGCCCCG.CCCAGGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc            <  1:3700075/101‑1 (MQ=18)
                                                                                            cccGCGCCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg           <  2:5101711/101‑1 (MQ=255)
                                                                                            cccCCNGCCAGCCCCCGCCCCG.CCCATGGCGGGGGT.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg           <  1:1870847/101‑1 (MQ=18)
                                                                                             ccTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGccccg          >  1:291024/1‑99 (MQ=255)
                                                                                              cccGGCCGGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCggg         <  1:9131973/99‑1 (MQ=255)
                                                                                               tCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGc        >  2:8090717/1‑101 (MQ=255)
                                                                                                  gCCGGCGCCCGCCCCG.CCCGTGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc     <  1:2759559/101‑1 (MQ=255)
                                                                                                  ccgaGCCCCCGCCCCG.CCCAGGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc     <  2:3951803/98‑1 (MQ=255)
                                                                                                   ccAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg    >  2:5916693/1‑101 (MQ=255)
                                                                                                     aGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCCCcggc  >  1:5380447/1‑101 (MQ=255)
                                                                                                     aGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGAGCCGGC.CTCTGCTGGGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcggc  >  1:2589890/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCAGCGCCCGCCCCG.CCCATGGCGGGCGC.TTCTCG.CCCG.CCGGGGCGGGCGCCGGC.CTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG