Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,178 C→A 1.9% intergenic (‑161/+131) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1780C→A 1.9% 3.2 ‑3.3 ‑0.0309intergenic (‑161/+131)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (169/134):  total (175/134)
Fisher's exact test strand distribution p-value = 3.80e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.17e-01

Only 100 of 411 total aligned reads displayed.

ACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCA  >  NC_014034/3068078‑3068278
                                                                                                      |                                                                                                      
 cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCa                                                                                                       >  1:2632500/1‑101 (MQ=255)
  gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCAt                                                                                                      <  2:2807842/101‑1 (MQ=255)
   aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATg                                                                                                     >  2:3830128/1‑101 (MQ=255)
   aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATg                                                                                                     <  2:6005396/101‑1 (MQ=255)
    tGGGCAGCCTGACCGAGGCCATCGCCGAGGCCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATgg                                                                                                    <  1:2940178/101‑1 (MQ=255)
     gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATggc                                                                                                   >  1:8148026/1‑101 (MQ=255)
     gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATggc                                                                                                   >  2:1695092/1‑101 (MQ=255)
        cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATggc.ggg                                                                                               >  2:3232601/1‑101 (MQ=255)
         aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATggc.gggc                                                                                              >  2:1133179/1‑101 (MQ=255)
                ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.Ttctc                                                                                      <  1:3014571/101‑1 (MQ=255)
                ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.Ctctc                                                                                      >  2:8424230/1‑101 (MQ=255)
                  gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.Ttctccc                                                                                    >  1:4938790/1‑99 (MQ=255)
                  gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.Ttctccc                                                                                    >  1:6133416/1‑99 (MQ=255)
                  gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcgc                                                                                    <  2:2444130/101‑1 (MQ=255)
                  gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcgc                                                                                    >  2:7155858/1‑101 (MQ=255)
                       cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcgcccgcc                                                                               >  1:4779698/1‑101 (MQ=255)
                        aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTcgcccgccc                                                                              >  1:6355244/1‑100 (MQ=255)
                          cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCcgg                                                                            >  1:3362363/1‑101 (MQ=255)
                           gCCGAGGTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGGCCCTCGGCC.T.GCGCCCGCCCCGCCATCGGC.GGGGGC.GCCCcgccccccccgg                                                                           >  1:7799811/1‑97 (MQ=18)
                             cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGGCCGCCCCGCCCATGGC.GGGGGC.TTCTTGGCCGCCGgggcg                                                                         >  2:4211547/1‑101 (MQ=18)
                              gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGgggcgg                                                                        >  1:2127396/1‑101 (MQ=255)
                               aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGgggcggg                                                                       >  1:605431/1‑101 (MQ=255)
                               aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGgggcggg                                                                       >  1:6936080/1‑101 (MQ=255)
                                ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGgggcgggg                                                                      >  2:6923777/1‑100 (MQ=255)
                                 gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCCGggcgggcg                                                                     >  1:5727189/1‑101 (MQ=255)
                                   cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGcc                                                                   <  2:5849200/101‑1 (MQ=255)
                                   cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGcc                                                                   >  1:619459/1‑101 (MQ=255)
                                    aggCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCg                                                                  <  2:6354995/99‑1 (MQ=255)
                                    aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCg                                                                  <  1:8891709/101‑1 (MQ=255)
                                    aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCCGGGCGGGCGCCg                                                                  >  2:3904493/1‑101 (MQ=255)
                                    aTGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCg                                                                  <  1:2397817/101‑1 (MQ=255)
                                        ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGccc                                                              >  1:1696942/1‑100 (MQ=255)
                                          aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCCGccccc                                                            >  1:8012181/1‑98 (MQ=255)
                                           acggTGGCCTCGTGCCCGCCGGGGGCCCAAGGCCCCTCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctg                                                           <  1:7592635/98‑1 (MQ=11)
                                            cAGTGCCCTCGGGCCCGCCGGGGCCCCAAAGCCCCTCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                          <  1:3215453/101‑1 (MQ=11)
                                             ggaggCCCCGGGACCGCCGAGGGCCCAAAGGCCCGCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgct                                                         <  1:3879383/98‑1 (MQ=14)
                                             agaagtcccGTGACCGCCGGGGGCCCAAAGGCCCTCGGCC.T.GCGCCCGCCCCGCCATCGGC.GGGCGCGTTCCCGCCCCCCGGGGTGCGGGCCGGGCCctgc                                                          >  2:7411924/9‑101 (MQ=14)
                                             aatGGCCCCGTGACCGGCGAGGGCCCAAAGGCCCTCGGCC.T.GCGCCCGCCCCGCCATCGGCGGGGGGC.GTCCCGCCGCCCGGGGCGGGCGGCggacccccg                                                          >  2:1615408/3‑94 (MQ=14)
                                             aGTGGCCTCTTGACCGCGGGGGCCCCAAGGCCCCTCGGCC.G.CCGCCCGCCCCGCCGATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgct                                                         <  2:5708423/101‑1 (MQ=11)
                                               tGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                                       <  1:1059178/101‑1 (MQ=255)
                                               tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                                       >  1:7459814/1‑101 (MQ=255)
                                                ggCCTCGGGCCCGCCGAGGCCCCAAGGCCCCGCGCCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                                      <  1:2027913/101‑1 (MQ=14)
                                                     catgccagccgGGGGCCCAAAGGCCGCCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.CTCTCGCCCGCCCGGGCCGGGGGCGGGCCCGGGCGCGCCTgg                                                 >  2:205033/8‑101 (MQ=14)
                                                     cGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTgg                                                 >  1:355807/1‑101 (MQ=255)
                                                     cGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCCCTGCTGCGCCTgg                                                 >  1:1212456/1‑101 (MQ=255)
                                                        gcccgccgAGGGCCCAAGGCCCCTCGCCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGa                                              <  1:2776043/99‑1 (MQ=255)
                                                        gcccgcccGGGCCCCAACGCCCCTCGCCC.G.CCCCCCGCCCCGCCCCTGGC.GGGCGT.TTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGa                                              <  2:1354777/99‑1 (MQ=9)
                                                          gccccggaggCCCCAGGGCCCCAGGCC.AGGCCCCCGCCCCGCCCGGGCC.GGGCGC.TTCCCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGaa                                             <  1:9196029/93‑1 (MQ=255)
                                                          ccgccgAGGCCCAAAGGGCCCTCGCCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                                            <  2:1966920/101‑1 (MQ=17)
                                                            gccgGGGGCCCAAAGCCCCGCGGCC.G.GCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                          <  1:8280545/101‑1 (MQ=14)
                                                            gccgAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                          >  2:8930509/1‑101 (MQ=255)
                                                            gccgAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                          >  1:2881133/1‑101 (MQ=255)
                                                            gccgAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGAAACg                                          >  1:718811/1‑101 (MQ=255)
                                                             ccgGGGGCCCAAGGGCCCCCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACgg                                         <  1:1899389/101‑1 (MQ=17)
                                                             cccggggCCAAAAGGCCCCCGGCCGG.GGGCCCGCCCCGCCCATGCC.GGGCGC.TTCTCCCCCGCCGGGGCGGCCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                          <  1:412962/97‑1 (MQ=14)
                                                              cgAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCCGCCTCTGCCGCGCCTGGGGGAACggg                                        >  2:3065889/1‑100 (MQ=17)
                                                              cgAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCCGCCTCTGCTGCGCCTGGGGGAACggg                                        >  1:7737329/1‑100 (MQ=255)
                                                               gAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTGTCGCCCGCCGGGGGCGGCGCCCGCCGCCGCTGCGCCTCGGGAGACGGtg                                       >  2:5599702/1‑100 (MQ=14)
                                                               gAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGGAACGGTc                                       >  1:6648065/1‑101 (MQ=255)
                                                                ggggCCAAAAGGCCCTCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCt                                      <  2:3687380/100‑1 (MQ=255)
                                                                aGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCt                                      >  1:5460647/1‑101 (MQ=255)
                                                                 gggCCAAAGGGCCCCCGGCC.A.GCGCCCCCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                     <  1:509586/101‑1 (MQ=17)
                                                                 ggccccAAAGCCCCGCGGCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                     <  2:4935232/98‑1 (MQ=17)
                                                                  ggCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGAGACGGGCTTg                                    >  2:6055662/1‑101 (MQ=255)
                                                                  ggCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCCGCGCCTGGGGGAACGGGCTTg                                    >  1:1839066/1‑101 (MQ=17)
                                                                   gCCCAAGGGCCCGCGGCC.C.GCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                                   <  2:8277138/101‑1 (MQ=18)
                                                                    cccAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGG.GGGCGC.TTCTCGCCCGCCGGGGCGAGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                                  >  1:6573064/1‑101 (MQ=255)
                                                                     ccAAGGGCCCTCGCCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                                 <  2:7794016/101‑1 (MQ=255)
                                                                       aaggccccTCGGCC.G.GCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc                               <  2:5379155/96‑1 (MQ=255)
                                                                       aaggccccGCGGCC.A.GCCCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc                               <  2:681902/96‑1 (MQ=255)
                                                                       aaaGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCAcc                               >  1:8779220/1‑101 (MQ=255)
                                                                       aaaGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCAcc                               >  1:6853436/1‑101 (MQ=255)
                                                                         aGGCCCTCGCCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGc                             <  1:379562/101‑1 (MQ=255)
                                                                          ggCCCTGGCCC.A.GCGCCCGCCCCGCCCATGGG.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                            <  1:1409951/101‑1 (MQ=255)
                                                                          ggCCCCCGGCC.G.GCGCCCCCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                            <  2:3332793/101‑1 (MQ=255)
                                                                          gccccTCGCCC.G.GCGCCCGCCCCGCCCATGGC.GGCCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                            <  2:7600272/99‑1 (MQ=255)
                                                                           gCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCCGCGCCTGGGGGAACCGTCTTGGCCCCCccc                           >  1:8956468/1‑101 (MQ=17)
                                                                           ccccCCGCCC.G.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc                           <  1:4429066/100‑1 (MQ=255)
                                                                               tCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGc                       >  2:8090717/1‑101 (MQ=255)
                                                                                gagCC.A.CCTCCCCCCGCCCCCTTGGG.GGGCGC.TTCTCCCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCt                      <  1:2275044/99‑1 (MQ=18)
                                                                                cGGCC.A.GCGCCCGCCCCGCCCATGGT.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGCt                      >  2:4563032/1‑101 (MQ=255)
                                                                                cGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGGACCGCCCGGGCt                      >  1:8595220/1‑101 (MQ=255)
                                                                                   gcccggccccccccccgcCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTc                     <  2:3026880/93‑1 (MQ=255)
                                                                                    gccgcccccccccccgcCCAGGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                    <  1:1733369/94‑1 (MQ=18)
                                                                                     cccgcgcCCCCCCCCCCCAGGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                   <  2:8738662/98‑1 (MQ=18)
                                                                                      a.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCCCcggc                 >  1:5380447/1‑101 (MQ=255)
                                                                                          gcccgcccCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcggg              >  2:8315558/1‑101 (MQ=255)
                                                                                               ccccgcccAGGGG.GGGCCC.TTCTGGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg         <  1:1609975/101‑1 (MQ=25)
                                                                                               ccccgcccAGGGC.GGGCGC.TTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg         <  2:2588427/101‑1 (MQ=255)
                                                                                               ccccgcccAGGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg         <  1:7276671/101‑1 (MQ=255)
                                                                                                  cgcccGTGCG.GGCCGT.TTCGCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa      <  2:5299247/101‑1 (MQ=25)
                                                                                                  cgcccATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa      <  2:7058445/101‑1 (MQ=255)
                                                                                                  cgcccAGGGC.GGGGGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa      <  1:3699668/101‑1 (MQ=255)
                                                                                                  cgcccAGGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa      <  1:7644994/101‑1 (MQ=255)
                                                                                                   gcccGTGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAg     <  2:8508090/101‑1 (MQ=255)
                                                                                                   gcccATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAg     >  2:6543275/1‑101 (MQ=255)
                                                                                                    cccATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAgg    >  1:8483835/1‑101 (MQ=255)
                                                                                                     ccATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc   >  2:370104/1‑101 (MQ=255)
                                                                                                      cATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCa  <  2:1854376/101‑1 (MQ=255)
                                                                                                      cATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGCa  >  2:351387/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
ACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.A.GCGCCCGCCCCGCCCATGGC.GGGCGC.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCA  >  NC_014034/3068078‑3068278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG