Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,179 A→T 1.9% intergenic (‑162/+130) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1790A→T 1.9% 6.9 ‑3.1 ‑0.8320intergenic (‑162/+130)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (181/133):  total (187/133)
Fisher's exact test strand distribution p-value = 4.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.74e-01

Only 100 of 412 total aligned reads displayed.

CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279
                                                                                                      |                                                                                                     
cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCa                                                                                                       >  1:2632500/1‑101 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCAt                                                                                                      <  1:764135/101‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATg                                                                                                     >  2:1426861/1‑101 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATg                                                                                                     <  2:6005396/101‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATg                                                                                                     >  2:1733316/1‑101 (MQ=255)
   tGGGCAGCCTGACCGAGGCCATCGCCGAGGCCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATgg                                                                                                    <  1:2940178/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATggc                                                                                                   >  1:3951918/1‑101 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATggc                                                                                                   <  1:6075451/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGA.GGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCCTggc.g                                                                                                 >  2:7228203/1‑101 (MQ=255)
     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATggc.g                                                                                                 <  1:6625240/101‑1 (MQ=255)
             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTtc                                                                                         <  1:5218211/101‑1 (MQ=255)
             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCCtc                                                                                         >  1:3454767/1‑101 (MQ=255)
               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTtctc                                                                                       <  1:3014571/101‑1 (MQ=255)
               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTtctc                                                                                       <  1:3806129/101‑1 (MQ=255)
               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCCtctc                                                                                       >  2:8424230/1‑101 (MQ=255)
                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTcgc                                                                                     >  2:5729042/1‑101 (MQ=255)
                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTcccc                                                                                    >  1:7551718/1‑101 (MQ=255)
                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTcgccc                                                                                   <  1:7780299/101‑1 (MQ=255)
                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTcgcccgcc                                                                                <  1:6914692/101‑1 (MQ=255)
                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCcgg                                                                             >  2:3699559/1‑101 (MQ=255)
                             gAGGTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGG.CCCTCGGCCT.GCGCCCGCCCCGCCATCGGCGGGGGGCGTCCGGCCCCCCGgggcg                                                                          >  1:5018163/1‑101 (MQ=18)
                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGgggcgg                                                                         >  1:8320261/1‑101 (MQ=255)
                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGgggcgggg                                                                       >  2:379554/1‑100 (MQ=255)
                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGGGCCTCTCGCCCGCCCGggcgggcggc                                                                    >  2:6371486/1‑99 (MQ=255)
                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGcc                                                                    <  1:8058045/101‑1 (MQ=255)
                                   aTGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCg                                                                   <  1:2397817/101‑1 (MQ=255)
                                    tGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCCGGGCGGGCGcccg                                                                  >  2:3496210/1‑99 (MQ=255)
                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGc                                                                 <  2:2159677/101‑1 (MQ=255)
                                      cgcaaCAGTGGCCTCGTGACCGCCGAGGGCCCAACGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGcc                                                                <  2:7447161/98‑1 (MQ=255)
                                      cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCgggc                                                                >  1:7878682/1‑99 (MQ=255)
                                       ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGccc                                                               >  1:1696942/1‑100 (MQ=255)
                                        gAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                              <  1:7049398/101‑1 (MQ=255)
                                         atgaGTGCCCTCTTGCCCGCGGGGGGCCCAAAGG.CCCTCGCCCA.GCCCCCCCCCCGCCCATGGC.GGGCGCTTCTCCCCCGCCGGGGGGGGCGCCGGCctct                                                             <  2:5627438/98‑1 (MQ=11)
                                          aCAGGGGCCTCGTGCCCGCCGAGGGCCCAAGGG.CCCTCGGCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctg                                                            <  2:5407538/101‑1 (MQ=14)
                                           cggTGGCCTCGGGCCCGCCGGGGGCCAAAAGC.CCCTCGCCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                           <  1:6786758/99‑1 (MQ=14)
                                            aGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCCTCTCGCCCGCCGGGGCGGGGGCCGGCCCctgct                                                          >  1:7204211/1‑101 (MQ=255)
                                            aGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCCCCCGCCCCGCCCATGGC.GGGCGCCTCACTCCCGCCGGGGCCGGCGCCGGCcacttcg                                                          >  2:12736/1‑98 (MQ=14)
                                            aGTGCCCTGGTCCCCGCGGAGGGCCCAAAGG.CCCTCGGCGG.GCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgct                                                          <  2:7080924/101‑1 (MQ=14)
                                              tGGCCTCGTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                                        <  1:1059178/101‑1 (MQ=255)
                                               ggCCTCGGGCCCGCCGAGGCCCCAAGGC.CCCGCGCCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                                       <  1:2027913/101‑1 (MQ=14)
                                               gcccccgggcccgccgAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                                       <  1:823148/91‑1 (MQ=255)
                                                    cGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCCCTGCTGCGCCTgg                                                  >  1:1212456/1‑101 (MQ=255)
                                                    cGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGGGCCCGCCCCTGCTGCGCCCgg                                                  >  1:6156886/1‑101 (MQ=14)
                                                      gcaCCGCCGGGGCCCCAAGGC.CCCTCGCCCC.GCGCCCGCCCCGCCCAGGGC.GGGCGCTTCTCCCCCCCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgggg                                                <  2:2921002/99‑1 (MQ=14)
                                                       gcccgccgAGGGCCCAAGGC.CCCTCGCCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGa                                               <  1:2776043/99‑1 (MQ=255)
                                                        aCCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCggggga                                              >  2:3839797/1‑99 (MQ=255)
                                                          cgccgA.GGCCCAAAGGCCCCCCGGCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAAc                                            <  2:2599252/101‑1 (MQ=14)
                                                           gcggAGGGCCCAAGGC.CCCCCGGCCA.GCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                           <  1:3230921/98‑1 (MQ=17)
                                                           gccgAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                           >  2:8930509/1‑101 (MQ=255)
                                                           gccgAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                           >  1:2881133/1‑101 (MQ=255)
                                                           cccgAGGGCCCAACGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                           <  2:3309309/100‑1 (MQ=255)
                                                            ccgGGGGCCCAAGGG.CCCCCGGCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACgg                                          <  1:1899389/101‑1 (MQ=17)
                                                             cgAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCACGGC.GGGCGCGTCTCGCCCGCCCGGGCGGGCGCCCGCCCCTGCTGGGCCTGGGGGAAccct                                         >  1:5399641/1‑98 (MQ=14)
                                                              gggggCCCAAGGC.CCCTCGCCCA.GCCNCNGNCCCGCCCAGGGC.GGGCGCTTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTc                                        <  1:5863725/99‑1 (MQ=17)
                                                              gAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCCGGGGGAACgggc                                        >  1:6319527/1‑99 (MQ=255)
                                                                gggCCGAAAGG.CACACGGGCG.GCGCCCGCCCCGCCAATGGC.GGGCGGTGCGCCCCCGCCGGGGGGGCCGGCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                      <  2:1634810/101‑1 (MQ=9)
                                                                gggCCCAAAGGCCCCCCGGCCA.GCGCCCCCCCCGCCCATGGC.GGGCGCTTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCt                                       <  2:1874773/101‑1 (MQ=17)
                                                                gggCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGGCtt                                      >  1:3612135/1‑101 (MQ=255)
                                                                ggccccAGAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                      <  2:3072579/98‑1 (MQ=255)
                                                                 ggCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGAGACGGGCTTg                                     >  2:6055662/1‑101 (MQ=255)
                                                                 ggCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCCGCGCCTGGGGGAACGGGCTTg                                     >  1:1839066/1‑101 (MQ=17)
                                                                  gCCCAAGGG.CCCGCGGCCC.GCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                                    <  2:8277138/101‑1 (MQ=18)
                                                                  gCCCAAGGC.CCCTGGGCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                                    <  2:7008474/101‑1 (MQ=18)
                                                                  gCCAAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTgg                                    >  1:8803831/1‑101 (MQ=255)
                                                                   gcccaggg.CCCCCCGCCAGGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                                    <  2:1996168/95‑1 (MQ=17)
                                                                    ccAAGGG.CCCTCGCCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                                  <  2:7794016/101‑1 (MQ=255)
                                                                    ccAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGcc                                  >  1:4141436/1‑100 (MQ=255)
                                                                    ccAAAGC.CCCCCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                                  <  2:4628395/101‑1 (MQ=255)
                                                                       aaGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTTCTGCGCCTGGGGGAACGGGCTTGGGACCg                               >  2:5306511/1‑101 (MQ=17)
                                                                        aGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGc                              >  2:1114736/1‑101 (MQ=255)
                                                                        aGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGc                              >  1:8753824/1‑101 (MQ=255)
                                                                         gg.CCCTGGCCCA.GCGCCCGCCCCGCCCATGGG.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                             <  1:1409951/101‑1 (MQ=255)
                                                                         gg.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCCGCTGCGCCCGGGGGAACGGTCTTGGCACCGcc                             >  1:8508470/1‑101 (MQ=255)
                                                                          gccccTCGCCCG.GCGCCCGCCCCGCCCATGGC.GGCCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                             <  2:7600272/99‑1 (MQ=255)
                                                                          g.CCCCCGCCCG.GCGCCCGCCCCGCCCAGGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc                            <  1:3700075/101‑1 (MQ=18)
                                                                            cccGCGCCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg                           <  2:5101711/101‑1 (MQ=255)
                                                                             ccTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCCCCCgg                          >  2:3591811/1‑101 (MQ=255)
                                                                              cccGGCCG.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCggg                         <  1:9131973/99‑1 (MQ=255)
                                                                               tCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGc                        >  2:8090717/1‑101 (MQ=255)
                                                                                gagCCA.CCTCCCCCCGCCCCCTTGGG.GGGCGCTTCTCCCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCt                       <  1:2275044/99‑1 (MQ=18)
                                                                                cGGCCG.GCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCt                       <  2:253136/101‑1 (MQ=255)
                                                                                cGGCCA.GCGCCCGCCCCGCCCATGGC.GGGGGCTTCTCCCCCCCCGGGCCGGGCGCCGGCCTCTGTCACCCCTGGGGACACGGGCGCGGGACCACCCGTGcg                       >  1:2187124/1‑100 (MQ=14)
                                                                                 ggCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGCTc                      >  2:6835739/1‑101 (MQ=255)
                                                                                  ccga.GCCCCCGCCCCGCCCAGGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                     <  2:3951803/98‑1 (MQ=255)
                                                                                   gccgcccccccccccgcCCAGGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                     <  1:1733369/94‑1 (MQ=18)
                                                                                   cccggcccccgccccgcCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                     <  1:7293063/94‑1 (MQ=255)
                                                                                    cccgcgcCCCCCCCCCCCAGGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                    <  2:8738662/98‑1 (MQ=18)
                                                                                     a.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCTGGGGCAACGGTCTTGGCACCGCCCGGGCTCcggc                  >  1:6536491/1‑101 (MQ=255)
                                                                                       gcgcCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTAGCACCGCCCGGGCTCcggcg                 >  1:5633260/1‑101 (MQ=255)
                                                                                         gcccgcccCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcggg               >  2:8315558/1‑101 (MQ=255)
                                                                                            cgccccgcccAGGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:4519139/101‑1 (MQ=255)
                                                                                              ccccgcccGTGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg          <  2:3050861/101‑1 (MQ=255)
                                                                                              ccccgcccATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg          >  2:591243/1‑101 (MQ=255)
                                                                                              ccccgcccAGGGG.GGGCCCTTCTGGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCgggg          <  1:1609975/101‑1 (MQ=25)
                                                                                                ccgcccATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa        <  1:7734975/101‑1 (MQ=255)
                                                                                                  gcccGTGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAg      <  2:8508090/101‑1 (MQ=255)
                                                                                                   cccAGGGC.GGGCGCTTCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAgg     <  1:8930915/101‑1 (MQ=255)
                                                                                                    ccAGGGC.GGGCGCTTCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc    <  1:4874663/101‑1 (MQ=255)
                                                                                                     cATGGC.GGGCGTTTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCa   <  2:6668898/101‑1 (MQ=255)
                                                                                                      aTGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGAAACGGGCCTGGCACCCCCCGGGGTCCGGGGGGCGGGGAGAGGAAg  >  2:4240064/1‑101 (MQ=18)
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CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTCGGCCA.GCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG