Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,216,136 C→A 100% A65D (GCT→GAT)  dctS3 → C4‑dicarboxylate transport sensor protein DctS

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,216,1360C→A100.0% 1660.1 421A65D (GCT→GAT) dctS3C4‑dicarboxylate transport sensor protein DctS
Reads supporting (aligned to +/- strand):  new base (189/232):  ref base (0/0):  total (189/232)

Only 100 of 444 total aligned reads displayed.

GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233
                                                                                                  |                                                                                                 
gCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 <  2:5597212/101‑1 (MQ=255)
 cTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACtgatgc                                                                                                >  2:8795743/1‑101 (MQ=255)
  tggtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATgcg                                                                                               >  1:4271609/1‑101 (MQ=255)
  tggtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATgcg                                                                                               >  1:4863454/1‑101 (MQ=255)
  tggtggCGATCGCCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATgcg                                                                                               >  1:6545856/1‑101 (MQ=255)
    ggggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCt                                                                                             <  1:9072223/99‑1 (MQ=255)
      ggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCttt                                                                                           >  1:2683221/1‑101 (MQ=255)
      ggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCttt                                                                                           <  2:2392618/101‑1 (MQ=255)
       gCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTg                                                                                          <  1:6910458/101‑1 (MQ=255)
        ggATCGTCGCGCTGATGGCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACCGGCCACTGATGCGCTTTgg                                                                                         <  1:5953202/100‑1 (MQ=255)
        cGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTgg                                                                                         <  2:273601/101‑1 (MQ=255)
           tcgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTc                                                                                      <  2:4404780/101‑1 (MQ=255)
           tcgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTc                                                                                      <  1:6362210/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    <  1:2635780/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    <  2:4864469/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    >  2:4230077/1‑101 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    >  1:6743630/1‑101 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    >  2:3672792/1‑101 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  >  1:2512746/1‑101 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  <  1:7660204/101‑1 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  <  1:1867994/101‑1 (MQ=255)
                   cTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGAc                                                                              <  2:7439146/101‑1 (MQ=255)
                    tgatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACg                                                                             >  1:7941703/1‑101 (MQ=255)
                    tgatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACg                                                                             >  2:2444733/1‑101 (MQ=255)
                    tgatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACg                                                                             <  2:3855455/101‑1 (MQ=255)
                     gatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGc                                                                            <  1:27859/101‑1 (MQ=255)
                     gatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGc                                                                            >  1:1715669/1‑101 (MQ=255)
                      atgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCt                                                                           <  1:3556408/101‑1 (MQ=255)
                      atgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCt                                                                           >  1:5238341/1‑101 (MQ=255)
                       tgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTg                                                                          >  1:2321833/1‑101 (MQ=255)
                       ggaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTg                                                                          <  1:5088682/100‑1 (MQ=255)
                         aCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgcg                                                                        >  1:337517/1‑101 (MQ=255)
                           gCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCt                                                                      <  2:268505/101‑1 (MQ=255)
                            cTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCtt                                                                     <  2:8298773/101‑1 (MQ=255)
                             tGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCttt                                                                    >  1:8126194/1‑101 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   >  1:1608499/1‑101 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   <  1:3647216/101‑1 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   <  2:2571790/101‑1 (MQ=255)
                                tCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAg                                                                 <  1:3192845/101‑1 (MQ=255)
                                  ggCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAt                                                               >  2:1236952/1‑101 (MQ=255)
                                   gcgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATg                                                              <  1:4457376/101‑1 (MQ=255)
                                    gccgttgctCTCCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATgg                                                             <  2:5360726/96‑1 (MQ=255)
                                     gcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGc                                                            >  2:7989564/1‑101 (MQ=255)
                                     gcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGc                                                            >  1:1124876/1‑101 (MQ=255)
                                      cgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCg                                                           >  1:4648332/1‑101 (MQ=255)
                                      cgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCg                                                           >  1:8983945/1‑101 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  1:1445529/1‑101 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  2:85323/1‑101 (MQ=255)
                                          gctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGCTGGCGGTgg                                                       <  1:8799566/101‑1 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  1:824942/101‑1 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      >  2:3527492/1‑101 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  1:646824/101‑1 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  2:6879004/101‑1 (MQ=255)
                                            tcgtCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGAc                                                     <  2:3573719/98‑1 (MQ=255)
                                              ttCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGa                                                   >  2:3349313/1‑101 (MQ=255)
                                                cGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAgg                                                 <  1:8341802/101‑1 (MQ=255)
                                                  ccGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAt                                               <  2:6573212/101‑1 (MQ=255)
                                                   cGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtg                                              >  2:2196631/1‑101 (MQ=255)
                                                   cGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtg                                              <  1:2403588/101‑1 (MQ=255)
                                                      cgcgCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGc                                           >  1:3925785/1‑101 (MQ=255)
                                                        cgcAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTg                                         <  2:2351209/101‑1 (MQ=255)
                                                         gcAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTgg                                        <  1:574040/101‑1 (MQ=255)
                                                          cAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGt                                       >  2:6085329/1‑101 (MQ=255)
                                                          cAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGt                                       <  2:7263615/101‑1 (MQ=255)
                                                          cAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGt                                       <  1:2163773/101‑1 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGGCGAACAGACGCTGCGCTTTCAGATGCAGGCGCACGGGGAtggacggggg                                      >  2:2960330/1‑93 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGCCGAACAGACGCTGCGCTTCCAGATGGCGGTGGACGAGGATGTGCTggcg                                      >  2:4056393/1‑99 (MQ=255)
                                                            aTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGc                                     <  1:3035635/101‑1 (MQ=255)
                                                               cGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTc                                  <  1:3137077/101‑1 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 <  1:1274655/101‑1 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 >  2:4554091/1‑101 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 <  2:2049909/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               <  1:856653/101‑1 (MQ=255)
                                                                   gCCCACCCCGCCCGTGCGAAACTGGTCACTGATGCGCTTTGGGTCGAACAGTCGCTGCGTTTTCAGATGTCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              <  2:3167299/101‑1 (MQ=255)
                                                                   gCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCAAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              >  2:7818656/1‑101 (MQ=255)
                                                                     cAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgcgc                            >  1:7437637/1‑101 (MQ=255)
                                                                     cAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgcgc                            >  2:3780853/1‑101 (MQ=255)
                                                                      aaCCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCt                           >  1:212411/1‑101 (MQ=255)
                                                                      aaCCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCt                           <  2:1864959/101‑1 (MQ=255)
                                                                        ccGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTgg                         >  1:7160846/1‑101 (MQ=255)
                                                                         tccgatctggCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGa                        <  1:3747552/93‑1 (MQ=255)
                                                                          gcgcCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGAt                       <  2:150832/101‑1 (MQ=255)
                                                                             cccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGcc                    <  2:1351734/101‑1 (MQ=255)
                                                                               cGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGTGCTGGATGCCAg                  <  2:1269774/101‑1 (MQ=255)
                                                                               cGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAg                  <  2:8983523/101‑1 (MQ=255)
                                                                                 ggCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAgcg                >  2:1715476/1‑101 (MQ=255)
                                                                                  gCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAgcgc               <  2:5093674/101‑1 (MQ=255)
                                                                                    gAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCg             >  1:1024036/1‑101 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGcccg            >  2:5833654/1‑99 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgg            <  2:7104344/101‑1 (MQ=255)
                                                                                      aaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCggg           >  2:5994423/1‑101 (MQ=255)
                                                                                      aaCTGGCCACTGATGCGCTTTGGCTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCggg           >  2:6677403/1‑101 (MQ=255)
                                                                                       aCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgggg          >  2:7299885/1‑101 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  2:2139861/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      >  2:1078919/1‑101 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  1:6150976/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      >  1:1939930/1‑101 (MQ=255)
                                                                                             cACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGc    >  2:6221783/1‑101 (MQ=255)
                                                                                              aCTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCa   <  2:2783564/101‑1 (MQ=255)
                                                                                              aCTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCa   <  1:8430218/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG