Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	76.3	21	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (5/16):  ref base (0/0):  total (5/16)

CGCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGG  >  NC_014034/3223837‑3223953                                                                                                   |                   cgcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgg                  >  2:5993804/1‑101 (MQ=255) cgcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgg                  >  1:1971251/1‑101 (MQ=255)   cgcCGTCCCAGGCCCCCGCCGCATGCACGGAGCGGACCGCATCCTGCAGGCTTTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  2:6648010/101‑1 (MQ=255)   cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  1:6781026/101‑1 (MQ=255)   cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  1:2048622/101‑1 (MQ=255)   cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  1:4566332/101‑1 (MQ=255)     ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc              <  1:8614205/101‑1 (MQ=255)      cGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcg             <  1:6294996/101‑2 (MQ=255)        tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  2:8142786/101‑1 (MQ=255)        tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  1:8285298/101‑1 (MQ=255)        tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  2:2356779/101‑1 (MQ=255)        tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           >  2:6604061/1‑101 (MQ=255)        gccgaGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCCCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  1:5164435/97‑1 (MQ=255)        gcccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  2:7251781/100‑1 (MQ=255)         cccAGATCCCCGCCGCATGCACGGCCCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca          <  2:5230513/101‑2 (MQ=255)         cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca          >  1:6578352/1‑100 (MQ=255)         cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca          >  1:7101587/1‑100 (MQ=255)         cccAGAACCCCGCCGCATGCACGCACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCACAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca          <  2:5958628/101‑2 (MQ=255)          ccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa         <  2:4586439/101‑3 (MQ=255)          ccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa         >  1:8395100/1‑99 (MQ=255)           cAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag        <  1:5832010/101‑4 (MQ=255)           cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag        <  2:8122382/101‑4 (MQ=255)             gacccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcg      <  1:4487082/98‑6 (MQ=255)             gAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcccagcg      >  2:2033168/1‑96 (MQ=255)              aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgc     >  2:4575264/1‑95 (MQ=255)              aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgc     <  2:1691346/101‑7 (MQ=255)              aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGACCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGgcgccaagcgc     >  2:4227373/1‑95 (MQ=255)               aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc    >  2:8586651/1‑94 (MQ=255)                ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca   <  1:5994039/101‑9 (MQ=255)                 cccGCCGCAAGCACGGACCCACCCGCACCCCGCCGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag  <  2:8951383/101‑10 (MQ=255)                                                                                                   |                   CGCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGG  >  NC_014034/3223837‑3223953 Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG