Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,191 A→C 94.4% K445T (AAG→ACG)  recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1910A→C94.4% 30.1 ‑2.7 ‑0.2285K445T (AAG→ACG) recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (153/116):  ref base (5/11):  total (158/127)
Fisher's exact test strand distribution p-value = 6.75e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.04e-01

Only 100 of 291 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGCT.G.CCGA.G.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278
                                                                                         |                                                                                              
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.t                                                                                >  1:7183597/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.t                                                                                >  1:5929462/1‑101 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttg                                                                              >  2:6119080/1‑99 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGG.CCGA.g.ttg                                                                              <  2:5384092/101‑3 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttgct                                                                            >  1:1339006/1‑97 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAN.GGCTGG.CCGA.g.ttgctc                                                                           >  2:320291/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttgctc                                                                           >  1:4693013/1‑96 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttgctcgc                                                                         >  2:1388004/1‑94 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttgctcgc                                                                         >  1:7103778/1‑94 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.g.ttgctcgc                                                                         >  1:7057558/1‑94 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGccc                                                                        >  2:7054800/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCg                                                                       >  1:5684729/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGccccg                                                                      >  1:4126682/1‑99 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGccccg                                                                      >  2:8314791/1‑99 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCgg                                                                      >  1:3281797/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGG.CCGA.GTTGCTCGCCCgg                                                                      <  2:3651485/101‑1 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGG.TGCTCGCCCGGc                                                                     >  2:8810467/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGc                                                                     >  1:2625223/1‑101 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGcc                                                                    >  2:2518468/1‑100 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCa                                                                    >  2:5500169/1‑101 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCa                                                                    >  2:681229/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGG.CCGA.GTTGCTCGCCCGGCAgg                                                                  <  1:1007962/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGGCCGGCAggg                                                                 >  1:8174614/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGG.TGCTCGCCCGGCAgggg                                                                >  1:3240051/1‑100 (MQ=255)
                  cAAAGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGgcg                                                               >  2:3364436/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGG.TGCTCGCCCGGCAggggcg                                                              >  2:1846954/1‑98 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGgggc                                                              >  1:2329291/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGgcgc                                                              <  2:1121414/101‑1 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCCGCAGGgggc                                                              >  2:6312617/1‑101 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGG.CCGA.GTTGCTCGCCCGGCAGGGcgc.g                                                            <  1:6731448/101‑1 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGGGC.gg                                                           >  1:2013306/1‑101 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.gg                                                           <  1:3663118/101‑1 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.gggc                                                         <  2:4661913/101‑3 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCCggggggcggga                                                         >  2:4462824/1‑93 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.ggggac                                                       >  1:9174802/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.gggcac                                                       >  1:8923875/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.gggcac                                                       <  2:3782344/101‑5 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                                                     <  2:8187493/101‑1 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                                                     <  1:8632955/101‑1 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                                                     <  1:4060/101‑1 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                                                     <  1:143238/101‑1 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGGGCGGGCAc.ggg                                                    >  1:5955741/1‑100 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCCGGGCGCGGGCAc.gggg                                                   >  1:4185720/1‑99 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.ggggacgggcg                                                  >  1:4614257/1‑92 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGC.ggggacgggcgg                                                 >  2:8647793/1‑91 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccg                                              <  2:5283803/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccg                                              <  1:989393/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccccg                                             >  2:1192519/1‑99 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCCGCAGGGCGCGGGGACGGGcggcccgg                                             >  2:3585030/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggc                                            >  1:1021960/1‑101 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGAt                                        <  1:4472172/101‑1 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGAt                                        <  1:4662544/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATc                                       <  2:8831580/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCt                                      <  2:4121371/101‑1 (MQ=255)
                                           gcgGGTCGGACCCTGGCCGAGGCGAA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCt                                      <  1:5469316/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggccagatcg                                     >  1:6917794/1‑95 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTg                                     <  2:9130337/101‑1 (MQ=255)
                                             gggTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGGGGCCCGGCCGATCTGc                                    >  1:111214/1‑101 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGGGGCCCGGGCgggtggggg                                  >  1:4291619/1‑93 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCCCGGGCGGCCCGGCCGATCTGCGGc                                 >  2:6511387/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGGTCTGCGGc                                 >  1:7967074/1‑101 (MQ=255)
                                                 cTGCCCCTGGCCGAGGCGCAGG.TGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                <  2:7364744/101‑1 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGGCCGGCCGATCTGCGGCt                                >  2:9177144/1‑101 (MQ=255)
                                                   gACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCTCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                              <  1:4785321/101‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:3391891/96‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:6220503/96‑1 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGGACGGGCGGCCCGGCCGATCTGCGGCTgggccg                          >  2:557832/4‑97 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                          <  1:2375483/98‑1 (MQ=255)
                                                        tggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCggg                         >  2:7523995/3‑101 (MQ=255)
                                                        tggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                         <  1:1211983/99‑1 (MQ=255)
                                                         ggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgggg                        >  1:3404501/2‑100 (MQ=255)
                                                         ggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                        >  1:8374941/2‑101 (MQ=255)
                                                         ggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCCATCTGCGGCTGGGCGGGc                        >  2:5892003/2‑101 (MQ=255)
                                                              gaggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                    <  1:3712316/91‑1 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  2:5970267/92‑1 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  2:4251960/94‑1 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:7721580/94‑1 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:5742894/94‑1 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:6312857/94‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  1:1647444/95‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  2:3465322/95‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  1:5303443/95‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  1:849875/95‑1 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGTAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGGTCCCGGTGc               >  1:8223873/6‑101 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGCAC.GGCT.GCCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  1:6400575/96‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc              >  1:1744586/5‑101 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  1:3120351/97‑1 (MQ=255)
                                                                     agctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCCGCCGATCTGCGGCTGGACGGGCTCCTGGTGCCg             >  2:1974358/4‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCcgg           >  1:761327/2‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCCGATGCCcgg           >  2:3702254/2‑101 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc          <  1:7524319/101‑1 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTCATGCCCGgc          >  2:2139187/1‑101 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGATGCCCGgcg         >  2:478962/6‑101 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACCGGGTCCTGATGCCCGgcg         >  1:357266/6‑101 (MQ=255)
                                                                          gaacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCCGgggc        >  1:927513/5‑101 (MQ=255)
                                                                          gaacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcgc        >  2:7178017/5‑101 (MQ=255)
                                                                          gaacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGgcgc        >  1:2176518/5‑101 (MQ=255)
                                                                           aacccGCGATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:8043076/98‑1 (MQ=255)
                                                                               cgcgATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCCGATGCCGGGCGCcgcgc   >  2:1507761/1‑100 (MQ=255)
                                                                                gcgATGGCAC.GGCTGG.CCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:7411551/101‑1 (MQ=255)
                                                                                         |                                                                                              
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGCT.G.CCGA.G.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG