Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,204 T→C 100% C449C (TGT→TGC recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2040T→C100.0% 1023.7 275C449C (TGT→TGCrecJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (140/135):  ref base (0/0):  total (140/135)

Only 100 of 287 total aligned reads displayed.

GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGC.GC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256117‑3256293
                                                                                                 |                                                                                             
gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGccc                                                                                         >  2:7054800/1‑101 (MQ=255)
 cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCg                                                                                        >  1:5684729/1‑101 (MQ=255)
 cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCg                                                                                        <  2:5552424/101‑1 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                                                       >  1:9130759/1‑101 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                                                       >  1:8259399/1‑101 (MQ=255)
   tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGc                                                                                      <  2:5035689/101‑1 (MQ=255)
     tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAg                                                                                    <  2:4619532/101‑1 (MQ=255)
     tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAg                                                                                    <  1:8062664/101‑1 (MQ=255)
      gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAgg                                                                                   <  1:1007962/101‑1 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGgc                                                                                 >  1:3465426/1‑101 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGgc                                                                                 <  1:1352089/101‑1 (MQ=255)
          aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCCGCAGGgg.gc                                                                              >  2:6312617/1‑101 (MQ=255)
           aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCCCCCGGcccggcacg                                                                              >  1:2207682/1‑93 (MQ=255)
            gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGG.GC.gg                                                                           >  1:2013306/1‑101 (MQ=255)
            gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gg                                                                           >  1:6189194/1‑101 (MQ=255)
            gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gg                                                                           <  2:2655521/101‑1 (MQ=255)
             ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCCGGGC.GC.ggg                                                                          >  1:7032125/1‑100 (MQ=255)
             ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGG.GC.ggg                                                                          >  1:3631679/1‑100 (MQ=255)
              gcggcgGGCACCGCAGGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.T.TGTCGGCCGGCCGCGC.GC.gggc                                                                         >  1:8831986/1‑99 (MQ=255)
                ggcggGCGCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gggcac                                                                       <  1:4120857/101‑5 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gggcgc                                                                       >  2:1503573/1‑97 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gggcac                                                                       <  2:3239952/101‑5 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GC.gggcac                                                                       <  1:6879810/101‑5 (MQ=255)
                  cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.gg                                                                    >  1:3830887/1‑101 (MQ=255)
                  cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.gg                                                                    <  2:8187493/101‑1 (MQ=255)
                   gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.ggg                                                                   >  1:774849/1‑100 (MQ=255)
                   gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.ggg                                                                   <  1:6493890/101‑2 (MQ=255)
                    ggCACCGCATGCCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GACTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.gggc                                                                  <  2:5820961/101‑3 (MQ=255)
                     gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGG.GC.ggggccgggcg                                                                  >  2:2375416/1‑92 (MQ=255)
                     gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.gggcg                                                                 <  2:650420/101‑4 (MQ=255)
                      cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.c.gggcgg                                                                <  1:467437/101‑5 (MQ=255)
                         cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.G.TAGCTCGCCCGGCAGGGC.GCGGGCA.CGGGcggccc                                                              <  1:8203206/101‑1 (MQ=255)
                          gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGcggcccg                                                             <  1:8390760/101‑1 (MQ=255)
                          gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGcggcccg                                                             <  2:737034/101‑1 (MQ=255)
                          gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGcggcccg                                                             <  2:5283803/101‑1 (MQ=255)
                             tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGcggcccggcc                                                          <  1:2811982/101‑1 (MQ=255)
                              ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGGCCCGCCg                                                         >  1:3249588/1‑101 (MQ=255)
                              ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCg                                                         <  1:5867581/101‑1 (MQ=255)
                               gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCACC.GGGGGGCCGGCCgg                                                        >  2:8812268/1‑100 (MQ=255)
                               gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGa                                                        <  1:2136991/101‑1 (MQ=255)
                                  gcgGGTCGGACCCTGGCCGAGGCGAAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCt                                                     <  1:5469316/101‑1 (MQ=255)
                                   cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTg                                                    <  2:9130337/101‑1 (MQ=255)
                                    gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGGTCTgg                                                   >  2:8272902/1‑100 (MQ=255)
                                    gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGc                                                   >  1:4166783/1‑101 (MQ=255)
                                      gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGGGGCCCGGGCgggtggggg                                                 >  1:4291619/1‑93 (MQ=255)
                                      gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCgg                                                 >  2:8592640/1‑101 (MQ=255)
                                       tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGGGGCCCGGCCGATCCGCGGc                                                >  1:1034114/1‑101 (MQ=255)
                                       tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGc                                                >  1:8515453/1‑101 (MQ=255)
                                       tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGc                                                <  2:3386536/101‑1 (MQ=255)
                                       gcTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGc                                                <  1:3154997/100‑1 (MQ=255)
                                        cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGGCCGGCCGATCTGCGGCt                                               >  2:9177144/1‑101 (MQ=255)
                                        cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGcg                                               >  1:9196340/1‑100 (MQ=255)
                                        cTGACCCTGGCCGAGGCACAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                                               <  2:6113565/101‑1 (MQ=255)
                                         tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                                              <  2:8861345/101‑1 (MQ=255)
                                          gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.TCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  1:4785321/101‑1 (MQ=255)
                                          gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGCTgg                                             >  1:5705845/1‑101 (MQ=255)
                                            ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  1:3690324/96‑1 (MQ=255)
                                            ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  2:6220503/96‑1 (MQ=255)
                                             cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCg                                          >  2:1178752/5‑101 (MQ=255)
                                             cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                                          <  1:1192551/97‑1 (MQ=255)
                                               tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGGG.GGCA.CGGGCGGCCCGGGCGATCTGCGGcccggacgg                                        >  1:4658719/3‑93 (MQ=255)
                                               tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCggg                                        >  2:7523995/3‑101 (MQ=255)
                                                ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                                       <  1:982416/100‑1 (MQ=255)
                                                ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGAGCTGCGGCTCGGCgggg                                       >  2:5931872/2‑100 (MQ=255)
                                                      ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:4692864/93‑1 (MQ=255)
                                                      ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:367368/93‑1 (MQ=255)
                                                      ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:3561395/93‑1 (MQ=255)
                                                        cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                               <  1:1647444/95‑1 (MQ=255)
                                                        cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                               <  1:5303443/95‑1 (MQ=255)
                                                        cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGACGGGCGCCCGATg                               >  1:7073006/7‑101 (MQ=255)
                                                         gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                              <  2:6770616/96‑1 (MQ=255)
                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGGCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             >  2:1889441/5‑101 (MQ=255)
                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc                             >  1:1744586/5‑101 (MQ=255)
                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:3325308/97‑1 (MQ=255)
                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  2:1031963/97‑1 (MQ=255)
                                                            gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGGCCCGATGcccg                           >  2:7196321/3‑99 (MQ=255)
                                                             ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCcgg                          >  1:761327/2‑101 (MQ=255)
                                                             ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCCGATGCCcgg                          >  2:3702254/2‑101 (MQ=255)
                                                             ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                          <  2:1693099/100‑1 (MQ=255)
                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGGTGCCgggg                         >  1:4499795/1‑100 (MQ=255)
                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                         <  1:7524319/101‑1 (MQ=255)
                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                         >  2:3432102/1‑101 (MQ=255)
                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTCATGCCCGgc                         >  2:2139187/1‑101 (MQ=255)
                                                              tGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGCCCCCGATGCCGGgc                         >  1:717139/1‑101 (MQ=255)
                                                               ggaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCggggg                        >  2:1316151/6‑99 (MQ=255)
                                                               ggaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCggggg                        >  1:2358502/6‑99 (MQ=255)
                                                               ggaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACCGGGTCCTGATGCCCGgcg                        >  1:357266/6‑101 (MQ=255)
                                                               ggaacccGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGCTGCCCGgcc                        >  2:4415705/6‑100 (MQ=255)
                                                                 aacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGtgcc                      >  2:4246405/4‑101 (MQ=255)
                                                                 aacccGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGggcc                      >  1:632606/4‑101 (MQ=255)
                                                                 aacccGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACCGGCTCCCGATTGCGGGcgcc                      >  2:3621795/4‑101 (MQ=255)
                                                                    ccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:5491824/101‑1 (MQ=255)
                                                                      gcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  2:4013031/101‑1 (MQ=255)
                                                                      gcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  1:7411551/101‑1 (MQ=255)
                                                                                cggctggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  1:4520224/95‑1 (MQ=255)
                                                                                cggctggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  1:1253328/95‑1 (MQ=255)
                                                                                 ggctggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       >  2:8724203/6‑101 (MQ=255)
                                                                                 ggctggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  1:8810097/96‑1 (MQ=255)
                                                                                   ctggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     <  2:7919398/98‑1 (MQ=255)
                                                                                    tggCCGA.GTT.GCTCGCCCGGCAGGGC.GCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATc    >  1:8631642/3‑101 (MQ=255)
                                                                                                 |                                                                                             
GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGC.GC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256117‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG