Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,548	A→G	100%	V160A (GTG→GCG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,548	0	A→G	100.0%	274.9	73	V160A (GTG→GCG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (0/73):  ref base (0/0):  total (0/73)

TCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342450‑3342593                                                                                                   |                                              tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                             <  1:7778441/101‑1 (MQ=255) tCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcc                                             <  1:543836/101‑1 (MQ=255)  cgcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                            <  2:2285108/101‑1 (MQ=255)  cgcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                            <  2:1722276/101‑1 (MQ=255)  cgcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGccc                                            <  1:1315946/101‑1 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  1:8750147/101‑2 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  1:6617333/101‑2 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  2:2407236/101‑2 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  2:4599477/101‑2 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  2:8642873/101‑2 (MQ=255)   gcgGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGcccg                                           <  2:7537063/101‑2 (MQ=255)     ggcccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGc                                         <  2:4283435/98‑1 (MQ=255)     ggACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCCGCGCGGGGTTGCGCATGACGCCCCGc                                         >  1:6539518/1‑101 (MQ=255)     ggACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGc                                         <  1:4270762/101‑1 (MQ=255)     ggACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGc                                         <  2:7610185/101‑1 (MQ=255)     ggACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACAACGCCCGGc                                         <  1:8803615/101‑1 (MQ=255)      gACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCa                                        <  2:3520824/101‑1 (MQ=255)       aCCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAc                                       <  1:6925422/101‑1 (MQ=255)       aCCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAc                                       <  2:6288376/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                      <  1:3535354/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                      <  2:8586185/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                      <  1:3240980/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                      <  1:7566173/101‑1 (MQ=255)        ccGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAcc                                      <  2:6868527/101‑1 (MQ=255)         cGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCg                                     <  2:4308316/101‑1 (MQ=255)          gAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCgg                                    <  1:5009601/101‑1 (MQ=255)          gAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCgg                                    <  1:1957301/101‑1 (MQ=255)           aGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCggg                                   <  2:4329870/101‑1 (MQ=255)           aGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTAGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCggg                                   <  1:6552829/101‑1 (MQ=255)            ggCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCgggg                                  <  1:30844/101‑1 (MQ=255)             gCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGc                                 <  2:2226448/101‑1 (MQ=255)              ccAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGcc                                <  1:193855/101‑2 (MQ=255)               cAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGGCCGCGGGGACCCGCGCGGGCTTTGGCCCGACGCCCCGGACCGGGGccc                               >  2:3666671/1‑99 (MQ=255)               cAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGccg                               <  2:9029609/101‑3 (MQ=255)               cAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGccg                               <  2:1295529/101‑3 (MQ=255)                 gCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcg                             <  1:8302949/101‑2 (MQ=255)                 gCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcg                             <  1:8876681/101‑2 (MQ=255)                   gTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggc                           <  1:7096021/101‑1 (MQ=255)                    tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                          <  2:6539258/101‑1 (MQ=255)                    tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                          <  2:4068852/101‑1 (MQ=255)                    tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                          <  2:3662527/101‑1 (MQ=255)                     ctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCCGCACCGGGGCCgcggcgg                         >  1:5804021/1‑101 (MQ=255)                      tcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggc                        <  1:327930/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:4500604/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:4085688/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  1:8341504/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  1:5772317/101‑1 (MQ=255)                       cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:253383/101‑1 (MQ=255)                        ggggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccg                      <  2:7902330/101‑1 (MQ=255)                        ggggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccg                      <  1:3171515/101‑1 (MQ=255)                         gggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgc                     <  1:8025589/101‑1 (MQ=255)                          ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgcg                    <  2:635693/101‑1 (MQ=255)                             tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCTGCGGCCGCGGGt                 <  1:6604561/101‑1 (MQ=255)                             tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGt                 <  2:5391833/101‑1 (MQ=255)                             tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGt                 <  2:6975828/101‑1 (MQ=255)                             tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCCGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGt                 <  1:4379421/101‑1 (MQ=255)                             cgATCTGGTGCAGCGAGCGTTGCGGCGTCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCCCCGGGGCCGAGGCGGCCGCGGGt                 <  2:2844980/100‑1 (MQ=255)                               aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg               <  2:7812666/101‑1 (MQ=255)                                tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:3484749/101‑1 (MQ=255)                                tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:5181859/101‑1 (MQ=255)                                  tcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgg            <  1:2741334/101‑1 (MQ=255)                                  tcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgg            <  2:8347741/101‑1 (MQ=255)                                  tcGTTCAGCGAGGGTTGCGGCGGCAGCGGCNGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgg            <  1:3666780/101‑1 (MQ=255)                                   cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc           <  2:7714646/101‑1 (MQ=255)                                   cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc           <  2:5803786/101‑1 (MQ=255)                                   cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc           <  2:1521270/101‑1 (MQ=255)                                    ggtCAGCGAGGGTTGCGGCGCCAGCGTCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcg          <  2:4232275/99‑2 (MQ=255)                                        aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  2:3533767/101‑6 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:5555969/101‑7 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:434294/101‑7 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  1:4599586/101‑7 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  1:3085661/101‑7 (MQ=255)                                         gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:2291100/101‑7 (MQ=255)                                          cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg    <  1:3204027/101‑8 (MQ=255)                                           gAGGGTTGCGGCGGCAGCGGCTGGCCCGCAGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccgg   <  2:3844239/101‑9 (MQ=255)                                            aGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggc  <  1:3747704/101‑10 (MQ=255)                                            aGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggc  <  2:4478841/101‑10 (MQ=255)                                                                                                   |                                              TCGCGGACCGAGGCCAGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342450‑3342593 Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 39 ≤  ATCG