Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,880 G→A 100% P228L (CCG→CTG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,8800G→A100.0% 1571.0 404P228L (CCG→CTG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (202/202):  ref base (0/0):  total (202/202)

Only 100 of 420 total aligned reads displayed.

GGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398783‑3398977
                                                                                                 |                                                                                                 
ggCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGa                                                                                                >  1:1033776/1‑101 (MQ=255)
  cttgttgtcccTCGCCGCCCGGGCCGCTTCGGCTCCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  2:4243728/93‑1 (MQ=255)
  cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  1:6164488/101‑1 (MQ=255)
  cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  2:5182781/101‑1 (MQ=255)
  cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              >  2:2993628/1‑101 (MQ=255)
   aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATgg                                                                                             >  2:5316402/1‑101 (MQ=255)
    tGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGt                                                                                            <  2:6572486/101‑1 (MQ=255)
      tttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCg                                                                                          <  1:4178206/101‑1 (MQ=255)
      tttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCg                                                                                          >  1:8065171/1‑101 (MQ=255)
      tttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCg                                                                                          >  2:1234097/1‑101 (MQ=255)
        tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        >  1:5293955/1‑101 (MQ=255)
        tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        >  1:3243110/1‑101 (MQ=255)
           ccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     <  2:3835129/101‑1 (MQ=255)
           ccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     <  1:839802/101‑1 (MQ=255)
              tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  1:7087378/101‑1 (MQ=255)
               gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 >  2:4921829/1‑101 (MQ=255)
                 cgccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgtcg                                                                               <  1:5674895/101‑1 (MQ=255)
                   ccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGc                                                                             <  1:2688592/101‑1 (MQ=255)
                    cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            <  1:4584370/101‑1 (MQ=255)
                     gcggcggcgTCGGCTGCCGCCTCCGCNNNNNTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGa                                                                           <  1:1092626/101‑1 (MQ=255)
                      cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          <  1:6748395/101‑1 (MQ=255)
                      cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          <  1:9001120/101‑1 (MQ=255)
                       ggcggcgTGGGCTGCCGGCTCCGCGCTTTGTCATCACCCCCGCGCCCATCGCTTACCCTTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAg                                                                         <  2:3165387/101‑1 (MQ=255)
                       ggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAg                                                                         >  2:6893562/1‑101 (MQ=255)
                        gcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAgg                                                                        <  1:1367639/101‑1 (MQ=255)
                          ggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGcc                                                                      >  2:1692453/1‑101 (MQ=255)
                            cgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTc                                                                    <  1:1740246/101‑1 (MQ=255)
                             gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCTGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCg                                                                   >  2:4868304/1‑101 (MQ=255)
                             gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGCAGGCCTCg                                                                   >  2:6481465/1‑101 (MQ=255)
                             gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCg                                                                   <  2:3408723/101‑1 (MQ=255)
                              tCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGt                                                                  <  1:2767577/101‑1 (MQ=255)
                              tCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGt                                                                  <  1:773459/101‑1 (MQ=255)
                              tCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGt                                                                  <  2:4349683/101‑1 (MQ=255)
                                ggCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAg                                                                >  2:5739706/1‑101 (MQ=255)
                                  cTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGcc                                                              <  2:4055971/101‑1 (MQ=255)
                                  cTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGcc                                                              <  1:7932287/101‑1 (MQ=255)
                                   tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  1:4860914/1‑101 (MQ=255)
                                    gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            >  1:3094911/1‑101 (MQ=255)
                                    gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            >  2:8469376/1‑101 (MQ=255)
                                      cgccTCCGCCTTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGGCCgcg                                                          >  2:872653/1‑99 (MQ=255)
                                         cTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTg                                                       <  1:748517/101‑1 (MQ=255)
                                           ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     <  2:7506298/101‑1 (MQ=255)
                                           ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     >  1:984858/1‑101 (MQ=255)
                                               ccTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGAtcttg                                                 >  2:4756299/1‑99 (MQ=255)
                                                  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtcttggcg                                              >  2:6775557/1‑96 (MQ=255)
                                                   tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             >  1:884411/1‑101 (MQ=255)
                                                   tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             >  2:6292255/1‑101 (MQ=255)
                                                    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                            >  2:264051/1‑101 (MQ=255)
                                                     tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                           >  1:1646401/1‑101 (MQ=255)
                                                     tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                           <  2:3552469/101‑1 (MQ=255)
                                                      catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCGGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          <  2:8904926/101‑1 (MQ=255)
                                                       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         <  2:1033039/101‑1 (MQ=255)
                                                       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  1:7130736/1‑101 (MQ=255)
                                                        tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                        >  1:8279461/1‑101 (MQ=255)
                                                         caCCCCCGCGCCCATCTGTTACGCGTCCAGCTGGCGCGACAGGATGGTCTAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       <  2:7288531/101‑1 (MQ=255)
                                                         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       <  2:8064831/101‑1 (MQ=255)
                                                         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       >  2:198211/1‑101 (MQ=255)
                                                         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAGGGTCGAGAAATCGTCGGCGAAGGCCTCGTAGCGCGAGGTGATCTtggcggggccgg                                       >  2:1382142/1‑101 (MQ=255)
                                                           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     <  1:1967976/101‑1 (MQ=255)
                                                            ccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                    <  1:5109129/101‑1 (MQ=255)
                                                            ccccGCGCCCATCGCTTACGCCTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                    <  2:3651996/101‑1 (MQ=255)
                                                             cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                   >  1:1868628/1‑101 (MQ=255)
                                                              ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgcg                                  >  1:4286055/1‑99 (MQ=255)
                                                              ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  <  1:3818164/101‑1 (MQ=255)
                                                                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                              <  1:1243075/101‑1 (MQ=255)
                                                                   cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                             >  1:51786/1‑101 (MQ=255)
                                                                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            >  1:357611/1‑101 (MQ=255)
                                                                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            >  1:2593044/1‑101 (MQ=255)
                                                                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            >  1:555870/1‑101 (MQ=255)
                                                                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            <  1:6816084/101‑1 (MQ=255)
                                                                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            >  2:1772993/1‑101 (MQ=255)
                                                                      aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                          >  1:2352334/1‑101 (MQ=255)
                                                                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGGGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGCCGggagggggttg                         >  1:2924630/1‑94 (MQ=255)
                                                                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       <  2:7730796/101‑1 (MQ=255)
                                                                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:4018915/101‑1 (MQ=255)
                                                                            tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                    >  2:28049/1‑101 (MQ=255)
                                                                              cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                  >  1:3745640/1‑101 (MQ=255)
                                                                              cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                  >  1:5473343/1‑101 (MQ=255)
                                                                                ggTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:8293172/100‑1 (MQ=255)
                                                                                cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:2434316/101‑1 (MQ=255)
                                                                                cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                >  1:2508871/1‑101 (MQ=255)
                                                                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:1472183/101‑1 (MQ=255)
                                                                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTGGTAGGCGATGAc              <  1:6547046/101‑1 (MQ=255)
                                                                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc             >  1:3353845/1‑101 (MQ=255)
                                                                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc             <  1:1774546/101‑1 (MQ=255)
                                                                                     aGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgc           <  1:4130399/101‑1 (MQ=255)
                                                                                     aGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgc           >  1:862671/1‑101 (MQ=255)
                                                                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGGGCTTGTTGTAGGAGATGCccgcc          >  2:7088989/1‑101 (MQ=255)
                                                                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc          >  2:5636002/1‑101 (MQ=255)
                                                                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         >  2:5504098/1‑101 (MQ=255)
                                                                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         >  1:5864074/1‑101 (MQ=255)
                                                                                         ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg       <  2:390970/101‑1 (MQ=255)
                                                                                          gcgcGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGa      <  2:7364916/101‑1 (MQ=255)
                                                                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  2:6325045/101‑1 (MQ=255)
                                                                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:205700/101‑1 (MQ=255)
                                                                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    >  2:2162509/1‑101 (MQ=255)
                                                                                            gcgACAGGATGGGCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:7352222/101‑1 (MQ=255)
                                                                                            gcgACAGGATGGCCGAGAATTCGTCGGAGAAGGCCTTGAAGCACGAGGTGATCTTGTCGGCGTCGGACGAGAGATTGTTGTAGGCGATGACCGCCGGGATc    <  2:8665383/101‑1 (MQ=255)
                                                                                            gcgACAGGAGGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:4660071/101‑1 (MQ=255)
                                                                                             cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg   <  1:6481715/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
GGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398783‑3398977

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG