Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,788 A→G 3.0% intergenic (‑91/+72) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7880A→G 3.0% 11.8 ‑2.0 0.0357intergenic (‑91/+72)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (178/168):  total (181/176)
Fisher's exact test strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890
                                                                                                     |                                                                                                            
gcaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATa                                                                                                              <  1:1013082/101‑1 (MQ=255)
gcaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATa                                                                                                              <  1:310926/101‑1 (MQ=255)
gcaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATa                                                                                                              <  1:2400212/101‑1 (MQ=255)
 gaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATaa                                                                                                             <  2:1176009/100‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGG.ATTTGGGGGAAAATaa                                                                                                             >  1:6713195/1‑101 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATaa                                                                                                             <  1:8378875/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATaa                                                                                                             <  2:5314304/101‑1 (MQ=255)
  aggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGGGAGATaaa                                                                                                            >  1:5680653/1‑101 (MQ=255)
  aggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATaaa                                                                                                            <  2:7084028/101‑1 (MQ=255)
  aggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATaaa                                                                                                            <  2:8279838/101‑1 (MQ=255)
   ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGACGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAg                                                                                                           <  1:6445649/101‑1 (MQ=255)
    gcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAgg                                                                                                          <  2:2503381/101‑1 (MQ=255)
      tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGGAAGAAAAAaggg                                                                                                        >  1:361919/1‑101 (MQ=39)
      tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGG.ATTTTGGGGAAGAAAAAaggg                                                                                                        >  1:7751015/1‑101 (MQ=255)
       cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGG.ATTTTGGGGAAGATAAAggggg                                                                                                       >  1:8235180/1‑100 (MQ=255)
       cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc                                                                                                       <  1:8690433/101‑1 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTAATTTGGGGCAGGTAAAAggggt                                                                                                       >  2:4907432/1‑100 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.ttt                                                                                                   <  2:4557784/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.ttt                                                                                                   <  1:2813669/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.ttt                                                                                                   <  1:1291233/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.ttt                                                                                                   <  2:8435879/101‑1 (MQ=255)
            cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTTGGGGCAGATAAAAGGgg.tttgc                                                                                                 >  2:6165333/1‑101 (MQ=255)
            cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgc                                                                                                 <  1:4728859/101‑1 (MQ=255)
            cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgc                                                                                                 <  1:8775360/101‑1 (MQ=255)
            cggcgACCCTACACGGCGCGACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgc                                                                                                 <  1:7960050/101‑1 (MQ=255)
             ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGG.ATTTTGGGCAAGAAAAACGGgc.tttggt                                                                                                >  1:7268934/1‑99 (MQ=255)
             ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgct                                                                                                <  2:1326587/101‑1 (MQ=255)
             ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgct                                                                                                <  2:863565/101‑1 (MQ=255)
              gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgctt                                                                                               <  2:3768663/101‑1 (MQ=255)
               cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGgc.tttgcttt                                                                                              <  1:1774399/101‑1 (MQ=255)
                gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCtttt                                                                                             <  1:554104/101‑1 (MQ=255)
                  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTAt                                                                                           <  1:5001012/101‑1 (MQ=255)
                   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTTGGGGAAGATAAAAGGGG.TTTGCttttttt                                                                                          >  2:7470312/1‑98 (MQ=255)
                   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATc                                                                                          >  1:3222310/1‑101 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGGAAGATAAAGAGGC.TTTGGTTTTTTCt                                                                                         >  2:3428964/1‑101 (MQ=38)
                         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tccc                                                                                   <  1:2331062/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGGTTTTATCTT.Tcccc                                                                                  <  2:5710961/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tcccc                                                                                  <  1:5833657/101‑1 (MQ=255)
                          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tcccc                                                                                  <  2:1024880/101‑1 (MQ=255)
                           gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCa                                                                                 <  1:373688/101‑1 (MQ=255)
                              cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAat                                                                              <  1:407693/101‑1 (MQ=255)
                               gACCACATTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAata                                                                             <  2:2940677/101‑1 (MQ=255)
                                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAatat                                                                            <  1:7517738/101‑1 (MQ=255)
                                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATc                                                                           <  1:5080761/101‑1 (MQ=255)
                                   aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATccc                                                                         <  2:4231104/101‑1 (MQ=255)
                                     cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGGAAGATAAAGGGGG.TTTGGTTTTTTCTT.TTCCCAAATATccccg                                                                       >  1:680308/1‑99 (MQ=38)
                                     cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCgg                                                                       <  2:6922798/101‑1 (MQ=255)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTTCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTCCTTTTATCTT.TCCCCAAATATCCCgggg                                                                     <  1:8090007/101‑1 (MQ=255)
                                         cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGt                                                                   <  1:1415404/101‑1 (MQ=255)
                                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATACCgggggggg                                                                 >  2:6711433/1‑98 (MQ=255)
                                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..g                                                              <  2:8325456/101‑1 (MQ=255)
                                              gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGGAAGATAAAGGGGG.TTTGCTTTTATCTT.TCCCCAAATATCCCCGGGGGGG..ggg                                                            >  1:7051893/1‑101 (MQ=38)
                                              gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..ggg                                                            <  2:172699/101‑1 (MQ=255)
                                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTTGGGCAAGATAAAGGGGC.TTTGCTTTTTTTTT.TTCCCCAATATTCCCGGGGGGC..ggggg                                                          >  1:4777167/1‑101 (MQ=37)
                                                cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTTGGGCAAGATAAAGGGGC.TTTGCTTTTTTCTT.TCCCCCAAAATCCCGGGGGGGC..ggggg                                                          >  2:8848162/1‑101 (MQ=37)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGGAAGATAAAAGGGG.TTTGCTTTTATCTT.TTCCCAAATATCCCGGGGGTGC..gggggg                                                         >  2:6327011/1‑100 (MQ=37)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTTGGGGAAGATAAAAGGGG.TTTGCTTTTTTTTT.TTCCCAAAAATCCCCGGGGGGG..gggggg                                                         >  1:9152790/1‑100 (MQ=25)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGc                                                         <  1:8542827/101‑1 (MQ=255)
                                                  ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCa                                                        <  2:7983248/101‑1 (MQ=255)
                                                   gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTggatctggcg                                                         <  2:6587491/101‑10 (MQ=255)
                                                    aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTTGGGGAAGATAAAAGGGC.TTTGCTTTTTTTTT.TCCCCAAAAATTCCGGGGGGGG..GGGGGCagg                                                      >  1:7906068/1‑100 (MQ=25)
                                                    aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGGAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGCGGGGGC..GGGGGCgg.a                                                     >  2:9019060/1‑101 (MQ=37)
                                                     aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTTGGGGAAGATAAAGGGGG.TTTGCTTTTTTTTT.TCCCCCAATATCCCGGGGGGGG..GGGGGGag.ag                                                    >  1:6029699/1‑101 (MQ=255)
                                                     aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGT.ATTTGGGGCAAGATAAAGGGGG.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGGGC..GGGGGCgg.ag                                                    >  2:6512775/1‑101 (MQ=37)
                                                      ttCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGc                                                   <  2:5442295/101‑1 (MQ=255)
                                                             tGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCCcgcc                                           <  1:4169265/101‑2 (MQ=255)
                                                              gCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCCAATATCCCCGGGGGGC..GGGGGGAG.AGC.CCCcgccg                                          >  1:3381442/1‑99 (MQ=21)
                                                               ctttcGATGCCTCCGGCGGGGAT.ATTTGGGGGAAGATGAA.ACCC.ATCCCTTTCATCTT.GCCCCAAATATCCCGGGGGGCCCGGGGGGCAG.CGc.cccccgcc                                          >  2:281986/5‑97 (MQ=21)
                                                                tttcGATGCCTCCGGCGGGGGT.ATTTTGGGGCAGATGAA.AACC.ATCCCTTTCATCTT.GCCCCAAATATCCCCGGGGGCC..CGGGGGGG.AGCGCCCCCcgccc                                        >  2:2041497/4‑100 (MQ=21)
                                                                tttcGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATGAA.CCCC.ATCCCTTTCATCTT.GCCCCAAATATCCCGGGGGGCCCGGGGGGCAG.CGc.cccccgcca                                         <  2:3244208/98‑6 (MQ=21)
                                                                   cGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCCGGGGGGC..GGGGGCAG.AGC.CCCcgccgccacg                                     >  2:209123/1‑94 (MQ=21)
                                                                     aTGCCTCCGGCGGGGAT.ATTTGGGGCAAGATGAA.ACCC.CTCCCTTTCATCTT.GCCCCAAATATCCCGGGGGGCCCGGGGGGCAG.CGc.ccccccccccttcc                                    >  1:8944819/1‑91 (MQ=18)
                                                                     aTGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGGGC..GGGGGCAG.AGC.CCCCGcccccacggt                                   >  2:8077167/1‑95 (MQ=18)
                                                                       gCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGGGC..GGGGGCAG.AGC.CCC..CGCCGCCCCGGTTc                               >  2:1814265/1‑101 (MQ=18)
                                                                         cTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCGCGGTTCAc                             >  2:2544888/1‑101 (MQ=255)
                                                                                 gggAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                     >  2:5553004/1‑101 (MQ=255)
                                                                                  ggAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                    >  2:2265549/1‑101 (MQ=255)
                                                                                   gAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCg                   >  1:8471729/1‑101 (MQ=255)
                                                                                   gAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCg                   >  2:1564350/1‑101 (MQ=255)
                                                                                    at.atTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGt                  >  2:4447809/1‑101 (MQ=255)
                                                                                     t.atTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                 >  1:6247997/1‑101 (MQ=255)
                                                                                        tttGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtctc               >  1:222636/1‑101 (MQ=255)
                                                                                        gttGGGGAAAAAAAAAGGGG..TTTTTTTTTTTTTC.TCCCCCAAAATCCGGGGGGGGG..GGGGGGCC.CGC.CCCCCCGCCGCCACGGTTCACGCCGCGACCGtct                <  1:7132452/100‑1 (MQ=255)
                                                                                          tGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  2:2704565/1‑101 (MQ=255)
                                                                                          tGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  2:3995226/1‑101 (MQ=255)
                                                                                           gtggggaaagaaaGGGGCGTTTGGTTTT.TCTTCTCCCAAAATA.CGCGGGGG.GC..GGGGGGGGCAGC.CCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTcc              <  2:3560719/91‑1 (MQ=255)
                                                                                            gggCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg           >  2:7782745/1‑101 (MQ=255)
                                                                                              gCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc         >  1:1059926/1‑100 (MQ=255)
                                                                                              gCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc         >  2:4329804/1‑100 (MQ=255)
                                                                                              gCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt         >  1:1955743/1‑101 (MQ=255)
                                                                                               cAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc        >  2:4764794/1‑101 (MQ=255)
                                                                                                aaGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg       >  1:9011499/1‑101 (MQ=255)
                                                                                                  gATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc     >  2:3119065/1‑101 (MQ=255)
                                                                                                   aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa    >  1:2907221/1‑101 (MQ=255)
                                                                                                   aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa    >  2:8711388/1‑101 (MQ=255)
                                                                                                   aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCa    >  2:5831053/1‑101 (MQ=255)
                                                                                                    tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg   >  2:5951553/1‑101 (MQ=255)
                                                                                                    tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg   >  1:5303363/1‑101 (MQ=255)
                                                                                                    tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAg   >  1:2130798/1‑101 (MQ=255)
                                                                                                     aaaGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc  >  2:7315059/1‑101 (MQ=255)
                                                                                                     |                                                                                                            
GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAT.ATTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATATCCCGGGGGTGC..GGGGGCAG.AGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG