Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,793 G→C 2.6% intergenic (‑96/+67) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7930G→C 2.6% 10.9 ‑4.3 0.0375intergenic (‑96/+67)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (2/9):  ref base (205/158):  total (208/167)
Fisher's exact test strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

CTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGAC  >  NC_014034/3439693‑3439895
                                                                                                      |                                                                                                        
 tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGA.AAAAaggg                                                                                                         >  1:361919/1‑101 (MQ=39)
 tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGA.AAAAaggg                                                                                                         >  1:7751015/1‑101 (MQ=255)
 tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAgggg                                                                                                         <  1:8756835/101‑1 (MQ=255)
   aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAGA.TAAAaggggt                                                                                                       >  2:418992/1‑99 (MQ=255)
   aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgct                                                                                                       <  2:6113441/101‑1 (MQ=255)
    tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgctt                                                                                                      <  1:3174106/101‑1 (MQ=255)
     ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgcttt                                                                                                     <  2:273714/101‑1 (MQ=255)
     ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgcttt                                                                                                     <  2:8435879/101‑1 (MQ=255)
      gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgctttg                                                                                                    <  1:5071146/101‑1 (MQ=255)
        ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGGAGAA.AAAAGGGggtttgt                                                                                                   >  1:6343145/1‑100 (MQ=255)
        ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGA.TAAAGGGgctttgct                                                                                                  >  2:4393213/1‑101 (MQ=255)
        ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGgctttgct                                                                                                  <  2:863565/101‑1 (MQ=255)
           gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGA.TAAAGGGGGTTTGGtttt                                                                                               >  2:7897683/1‑101 (MQ=255)
           gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCtttt                                                                                               <  2:6447495/101‑1 (MQ=255)
           caCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGTCTGCGGCCGATGCCTGCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGCgcttgtctgtt                                                                                               <  2:5867572/100‑8 (MQ=38)
            aCCCTACCCGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTa                                                                                              <  1:336593/101‑1 (MQ=255)
            aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGA.TAAAAGGGGGTTGCttttt                                                                                              >  1:1672717/1‑100 (MQ=255)
             cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTAt                                                                                             <  2:5079062/101‑1 (MQ=255)
              ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGA.TAAAGGGGGTTTGCTTTTAtt                                                                                            >  1:2341124/1‑100 (MQ=255)
               cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAA.TTTTGGGCAAGG.TAAAAGGGGTTTGTTTTTTTCt                                                                                           >  1:2205568/1‑101 (MQ=255)
                tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCtt                                                                                          <  1:6132911/101‑1 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTc                                                                                        <  2:1520589/101‑1 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTc                                                                                        <  2:6309659/101‑1 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGC.TTTTGGGGAAAG.TAAAGGGGGTTTGGTTTTGTTTTTc                                                                                        >  1:2987290/1‑101 (MQ=255)
                   acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAGGGGCTTTGGTTTTATTTTTcc                                                                                       >  2:4903598/1‑101 (MQ=255)
                      gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                    <  2:4326926/101‑1 (MQ=255)
                      gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                    <  1:373688/101‑1 (MQ=255)
                      gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                    <  1:8536942/101‑1 (MQ=255)
                        gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGA.TAAAAGGGGTTTTCTTTTTTTTTTTCCCaaa                                                                                  >  2:5552733/1‑101 (MQ=37)
                         cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAat                                                                                 <  1:407693/101‑1 (MQ=255)
                          gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGA.TAAAGGGGGTTTGCTTTTTTCTTTCCCCAAata                                                                                >  1:8902952/1‑101 (MQ=255)
                          gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAata                                                                                <  2:2858119/101‑1 (MQ=255)
                           aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCTATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCGTTCCCCAAatat                                                                               <  1:8172045/101‑1 (MQ=255)
                           aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                               <  2:5813370/101‑1 (MQ=255)
                            ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATc                                                                              <  1:5897177/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATccc                                                                            <  2:8034285/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATccc                                                                            <  1:8646745/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAAA.TTTGGGGGAAGA.TAAAGGGGCTTTGCTTTTATCTTTTCCCAAATATccc                                                                            >  1:5589487/1‑101 (MQ=255)
                               gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGGTTTGCTTTTATCTTTCCCCCAATATTCCg                                                                           >  1:7374924/1‑101 (MQ=255)
                               gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCg                                                                           <  1:5478555/101‑1 (MQ=255)
                                 tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGAGCGA.CTTCGGGCGACACTAAGGGGGCTTAGTTTTATTCTTTCCCCaataaactgcg                                                                          >  2:4764670/1‑92 (MQ=255)
                                     gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGA.TAAAAGGGCTTTGCTTTTTTCTTTTCCCCAATATCCCggggggg                                                                     >  2:2330136/1‑99 (MQ=37)
                                     gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCggggggg                                                                     >  1:4937359/1‑99 (MQ=255)
                                     gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTg                                                                     >  2:777416/1‑101 (MQ=255)
                                      aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGGTTTGCTTTTATCTTTCCCCCAATATCCCGGGGGTGc                                                                    >  2:2211950/1‑101 (MQ=255)
                                      aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGc                                                                    <  1:4360606/101‑1 (MQ=255)
                                      aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATACCgggggggg                                                                    >  2:6711433/1‑98 (MQ=255)
                                       aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..g                                                                 <  2:5435723/101‑1 (MQ=255)
                                       aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..g                                                                 <  1:1355211/101‑1 (MQ=255)
                                        aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGA.TAAAGGGGCTTTGCTTTTATTTTTCCCCCAAAATCCCGGGGGGGC..gg                                                                >  1:2269451/1‑101 (MQ=255)
                                           cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGA.TAAAGGGGCTTTGCTTTTTTTTTTTCCCCAATATTCCCGGGGGGC..ggggg                                                             >  1:4777167/1‑101 (MQ=37)
                                           cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGA.TAAAGGGGCTTTGCTTTTTTCTTTCCCCCAAAATCCCGGGGGGGC..ggggg                                                             >  2:8848162/1‑101 (MQ=37)
                                            aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  2:1069331/101‑1 (MQ=255)
                                              gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTggatctggcg                                                            <  2:6587491/101‑10 (MQ=255)
                                               aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGGAAGA.TAAAAGGGCTTTGCTTTTTTTTTTCCCCAAAAATTCCGGGGGGGG..GGGGGCagg                                                         >  1:7906068/1‑100 (MQ=25)
                                               aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGCGGGGGC..GGGGGCgga                                                         >  2:9019060/1‑101 (MQ=37)
                                                  tCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGGAAGA.TAAAGGGGGTTTGCTTTTATATTTCCCCAAATATTCCGGGGGGGC..GGGGGGagaggc                                                      >  1:8421602/1‑99 (MQ=37)
                                                  tCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                      <  1:9009695/101‑1 (MQ=255)
                                                   cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAAGGGGTTTGCTTTTATCTTTTCCCCAACATCCCGGGGGGGC..GGGGGCAGAGccc                                                     >  1:8023470/1‑101 (MQ=37)
                                                    ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCCGGGGGGG..GGGGGGCGAGcccc                                                    >  2:720315/1‑101 (MQ=37)
                                                     gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAGGGGCTTTGCTTTTTTCTTTCCCCCAATATCCCGGGGGGGC..GGGGGCAGGGccccc                                                   >  1:6937224/1‑101 (MQ=37)
                                                      ccTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                  <  1:9200146/101‑1 (MQ=255)
                                                       tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TGAA.ACCCATCCCTTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                  <  2:257900/94‑2 (MQ=25)
                                                        tGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                <  2:1672670/101‑2 (MQ=255)
                                                           ggCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCCAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcc                                             >  1:8241177/1‑97 (MQ=21)
                                                              cGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGGCGAGCCCCcgccgccacg                                          >  2:1898846/1‑94 (MQ=21)
                                                               gATGCCTCCGGCGGGGCTA.CTTGGGGGTAGA.CAAATGGGCTTTTTTTCTTACTTTCCCCCAATATCCCTGGGGGGC..GGGGGC.GGGCCCCC.CGCCGCCAAgg                                       >  2:3196534/1‑101 (MQ=255)
                                                               gATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgccccgg                                         >  2:3434026/1‑93 (MQ=255)
                                                                           ggggATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                           >  1:6726246/1‑101 (MQ=255)
                                                                           ggggATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                           >  1:1030055/1‑101 (MQ=255)
                                                                            gggATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcgcc                          >  2:7383102/1‑99 (MQ=255)
                                                                             ggATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                         >  1:124399/1‑101 (MQ=255)
                                                                              gATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                        >  1:2505487/1‑101 (MQ=255)
                                                                              gATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                        >  1:4687925/1‑101 (MQ=255)
                                                                              gATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                        >  2:2062352/1‑101 (MQ=255)
                                                                                 tttgtGGGGGAAGG.AAAGGGGGTTTTTTTTTTTTCTCCCCCCAAATGGCGGGGGGGGGG..GGGGGGCG.GCCCCCCCGCCGCCACGGTTCACGCCGCGACCGt                       <  1:5827622/97‑1 (MQ=255)
                                                                                   gttGGGGAAAAA.AAAAGGGG.TTTTTTTTTTTTTCTCCCCCAAAATCCGGGGGGGGG..GGGGGGCCCGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGtct                     <  1:7132452/100‑1 (MQ=255)
                                                                                     tGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                  >  2:1773490/1‑101 (MQ=255)
                                                                                     tGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCAGCGACCGTCTCCg                  >  2:6917978/1‑101 (MQ=255)
                                                                                      ggggCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                 >  2:5645024/1‑101 (MQ=255)
                                                                                      ggggCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                 >  2:8288355/1‑101 (MQ=255)
                                                                                       gggCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                >  1:7057766/1‑101 (MQ=255)
                                                                                          cAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc             >  1:1865527/1‑101 (MQ=255)
                                                                                              a.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa         >  2:8711388/1‑101 (MQ=255)
                                                                                              a.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa         >  2:3803922/1‑101 (MQ=255)
                                                                                                tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        >  2:2531627/1‑101 (MQ=255)
                                                                                                tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        >  1:2036226/1‑101 (MQ=255)
                                                                                                tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        >  2:3067054/1‑101 (MQ=255)
                                                                                                tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        >  1:9122992/1‑101 (MQ=255)
                                                                                                 gagggggTTTTTCTTTTTACCTT.CCCACAAATGCCGGGGGGGG..GGGGGGGCGGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg        <  2:3645747/98‑1 (MQ=255)
                                                                                                 aaaGGGTTTTTTTTTTTTTCTCTCCCCAAATATGCCGGGGGGGG..GGGGGGCCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa         <  2:4052082/101‑1 (MQ=255)
                                                                                                 aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc       >  1:2846141/1‑101 (MQ=255)
                                                                                                 aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc       >  2:8204292/1‑101 (MQ=255)
                                                                                                 aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc       >  2:1272516/1‑101 (MQ=255)
                                                                                                 aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc       >  2:4499434/1‑101 (MQ=255)
                                                                                                   aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc     >  2:3304031/1‑101 (MQ=255)
                                                                                                   aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc     >  2:7537450/1‑101 (MQ=255)
                                                                                                   aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc     >  2:6971080/1‑101 (MQ=255)
                                                                                                   aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc     >  1:7718591/1‑101 (MQ=255)
                                                                                                     gggCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga   >  1:52683/1‑101 (MQ=255)
                                                                                                      |                                                                                                        
CTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGA.TAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGAC  >  NC_014034/3439693‑3439895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG