Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,794 G→C 3.2% intergenic (‑97/+66) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7940G→C 3.2% 12.8 ‑3.5 ‑0.0343intergenic (‑97/+66)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (203/129):  total (206/137)
Fisher's exact test strand distribution p-value = 3.03e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 472 total aligned reads displayed.

TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACG  >  NC_014034/3439694‑3439896
                                                                                                     |                                                                                                             
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGAAAAAaggg                                                                                                               >  1:361919/1‑101 (MQ=39)
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGAAAAAaggg                                                                                                               >  1:7751015/1‑101 (MQ=255)
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGAAGATAAAaggg                                                                                                               >  1:9059745/1‑101 (MQ=255)
tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAA.TTTTGGGGAAGATAAAaggg                                                                                                               >  2:8376165/1‑101 (MQ=255)
   tGGCGGCGACCCTACACGGCGCGACCAGCGTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAGATAAAAGGgg.tt                                                                                                           >  2:6528789/1‑101 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.ttt                                                                                                          <  2:5777704/101‑1 (MQ=255)
    ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.ttt                                                                                                          <  2:4557784/101‑1 (MQ=255)
     gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.tttg                                                                                                         <  1:5071146/101‑1 (MQ=255)
     gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.tttg                                                                                                         <  2:3432869/101‑1 (MQ=255)
     gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.tttg                                                                                                         <  1:2838814/101‑1 (MQ=255)
      cggcgACCCTACACGGCGCGACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.tttgc                                                                                                        <  1:7960050/101‑1 (MQ=255)
       ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGGAGAAAAAAGGGgg.tttgt                                                                                                        >  1:6343145/1‑100 (MQ=255)
       ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGCAAGAAAAACGGgc.tttggt                                                                                                       >  1:7268934/1‑99 (MQ=255)
        gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgc.tttgctt                                                                                                      <  2:3768663/101‑1 (MQ=255)
          gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCtttt                                                                                                    <  2:2850977/101‑1 (MQ=255)
           aCCCTACCCGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTa                                                                                                   <  1:336593/101‑1 (MQ=255)
           aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGAAGATAAAAGGGG.GTTGCttttt                                                                                                   >  1:1672717/1‑100 (MQ=255)
            cccTACAGCGCGCGACACGCGTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTAt                                                                                                  <  2:2972968/101‑1 (MQ=37)
            cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGAAGATAAAAGGGC.TTTGCtttttt                                                                                                  >  1:5206304/1‑99 (MQ=255)
             ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAAGGGG.TTTGCttttttt                                                                                                 >  2:7470312/1‑98 (MQ=255)
             ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGATAAAGGGGG.TTTGCTTTTAtt                                                                                                 >  1:2341124/1‑100 (MQ=255)
              cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGATAAAGGGGG.TTTGCTTTTTTCt                                                                                                >  1:2442788/1‑101 (MQ=39)
              cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAA.TTTTGGGCAAGGTAAAAGGGG.TTTGTTTTTTTCt                                                                                                >  1:2205568/1‑101 (MQ=255)
              cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCt                                                                                                <  1:8879657/101‑1 (MQ=255)
               tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCtt                                                                                               <  1:6132911/101‑1 (MQ=255)
                acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCtt.t                                                                                             <  1:8643493/101‑1 (MQ=255)
                 cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTGCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tc                                                                                            <  2:1060743/101‑1 (MQ=255)
                 cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tc                                                                                            <  1:7525142/101‑1 (MQ=255)
                   cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.Tccc                                                                                          <  1:809732/101‑1 (MQ=255)
                   cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGAGAAGATAAAGGGGC.TTTGCTTTTTTCTT.Tccc                                                                                          >  2:6756948/1‑101 (MQ=255)
                      cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCaa                                                                                       <  2:2525773/101‑1 (MQ=255)
                        ggtccAGCTTTCTAAAGACAGGAAGTCGGCCTGCGGCTGATTCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTCCTTTTATCTT.TCCCCAAat                                                                                     <  2:4975315/98‑1 (MQ=37)
                        cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAat                                                                                     <  1:407693/101‑1 (MQ=255)
                        cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAat                                                                                     <  2:3286458/101‑1 (MQ=255)
                        cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAat                                                                                     <  1:6823556/101‑1 (MQ=255)
                        cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAat                                                                                     <  2:8011075/101‑1 (MQ=255)
                          aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAata.t                                                                                  <  1:3724368/101‑1 (MQ=255)
                            cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.cc                                                                               <  2:1579261/101‑1 (MQ=255)
                             aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.ccc                                                                              <  2:6433093/101‑1 (MQ=255)
                             aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAAA.TTTGGGGGAAGATAAAGGGGC.TTTGCTTTTATCTT.TTCCCAAATA.T.ccc                                                                              >  1:5589487/1‑101 (MQ=255)
                                tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCACATA.T.CCCggg                                                                           >  2:5467548/1‑101 (MQ=255)
                                tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAAGGGC.TTTTCTTTTTTCTT.TTCCCCAAAA.T.TCCggg                                                                           >  1:5883538/1‑101 (MQ=37)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAGGGGG.TTTGCTTTTTTTTT.TCCCCCAATA.T.CCCcggg                                                                          >  1:9192619/1‑101 (MQ=255)
                                 ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAGAAAAAAGGGG.TTTGCTTTTTTCTT.TCCCCAAATA.T.TCCgggg                                                                          >  2:3189718/1‑101 (MQ=38)
                                  tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.ACCggggg                                                                         >  2:4283642/1‑101 (MQ=255)
                                   cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGt                                                                        <  2:2274518/101‑1 (MQ=255)
                                   cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAAGGGC.TTTGGTTTTTTCTT.TCCCCCAATA.TAACCggggg                                                                         >  2:437850/1‑101 (MQ=37)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCggggggg                                                                       >  1:4937359/1‑99 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTTGGGGGAGATAAAAGGGG.GTTGCTTTTTTCTT.TTCCCCAATATT.CCCgggggg                                                                        >  2:1893881/1‑100 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCcgggggg                                                                       >  2:2057967/1‑99 (MQ=255)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGG.TTTGCTTTTTTCTT.TTCCCAAATA.T.ACCggggggg                                                                       >  2:4802830/1‑99 (MQ=38)
                                    gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTg                                                                       <  2:8286717/101‑1 (MQ=255)
                                     aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGc                                                                      <  1:4360606/101‑1 (MQ=255)
                                      aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..g                                                                   <  1:1355211/101‑1 (MQ=255)
                                       aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAGGGGC.TTTGCTTTTATTTT.TCCCCCAAAA.T.CCCGGGGGGGC..gg                                                                  >  1:2269451/1‑101 (MQ=255)
                                        gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..ggg                                                                 <  2:8667106/101‑1 (MQ=255)
                                         aCAGGGATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAAA.TTTGGGGCAAGATAAAGGGGG.TTTGCTTTTTTTTT.TCCCCAAAAA.T.CCCGGGGGGGC..gggg                                                                >  1:5453641/1‑101 (MQ=37)
                                             gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCag                                                            <  1:3138952/101‑1 (MQ=255)
                                               aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCag.ag                                                         <  2:6216567/101‑1 (MQ=255)
                                                  cGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGATAAAGGGGG.TTTGCTTTTATTTT.TTCCCAAAAA.T.CCCGGGGGTGC..GGGGGGAG.GGcgc                                                      >  2:4483840/1‑99 (MQ=25)
                                                    gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCCAATA.T.CCCGGGGGTGC..GGGGGCAG.AGccccc                                                    >  2:7110040/1‑101 (MQ=255)
                                                     ccTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCGCCcg                                                   <  2:788429/101‑1 (MQ=255)
                                                      tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCC.ATCCCTTTCATCTT.GCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAG.CGcccccc                                                   <  1:4760667/94‑2 (MQ=25)
                                                      tgactttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.ACCC.ATCCCTTTCATCTT.GCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAG.CGcccccc                                                   <  1:66026/94‑2 (MQ=25)
                                                          ggCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCCAATA.T.CCCGGGGGGGC..GGGGGCAG.AGCCCCcgccgcc                                              >  1:8241177/1‑97 (MQ=21)
                                                            ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCCcgccgcccc                                            >  2:8796533/1‑95 (MQ=255)
                                                               aTGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGGGC..GGGGGCAG.AGCCCCCGcccccacggt                                         >  2:8077167/1‑95 (MQ=18)
                                                                 gCCTCCGGCGGGGATA.TTTGGGGGGAGATAAAAGGGG.TTTGCTTTTATCTT.TTCCCCAATA.T.ACCCGGGGTGC..GGGGGCAG.GGGCCCC.C.CCGCCACgggtt                                     >  2:1740689/1‑98 (MQ=16)
                                                                   cTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCGCGGTTCAc                                   >  2:2544888/1‑101 (MQ=255)
                                                                     ccGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCAcgc                                 >  1:7868494/1‑101 (MQ=255)
                                                                       ggcggGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCAcgccg                               >  1:9004591/1‑101 (MQ=255)
                                                                          ggggATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGa                            >  1:1030055/1‑101 (MQ=255)
                                                                          ggggATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGa                            >  1:8737506/1‑101 (MQ=255)
                                                                            ggATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                          >  1:124399/1‑101 (MQ=255)
                                                                               ta.tTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                       >  1:459610/1‑101 (MQ=255)
                                                                                tttgtGGGGGAAGGAAAGGGGGT.TTTTTTTTTTTCTC.CCCCCAAATG.G.CGGGGGGGGGG..GGGGGGCG..GCCCCCCCGCCGCCACGGTTCACGCCGCGACCGt                        <  1:5827622/97‑1 (MQ=255)
                                                                                  gttGGGGAAAAAAAAAGGGG..TTTTTTTTTTTTTC.TCCCCCAAAA.T.CCGGGGGGGGG..GGGGGGCC.CGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGtct                      <  1:7132452/100‑1 (MQ=255)
                                                                                   ttGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                    >  2:1262745/1‑101 (MQ=255)
                                                                                    tGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                   >  2:3995226/1‑101 (MQ=255)
                                                                                    tGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                   >  2:2704565/1‑101 (MQ=255)
                                                                                     gtggggaaagaaaGGGGCGTTTGGTTTT.TCTTCTCCCAAAATA...CGCGGGGG.GC..GGGGGGGGCAGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTcc                    <  2:3560719/91‑1 (MQ=255)
                                                                                      gggCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                 >  1:7057766/1‑101 (MQ=255)
                                                                                         cAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc              >  1:1865527/1‑101 (MQ=255)
                                                                                         cAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc              >  1:85075/1‑101 (MQ=255)
                                                                                          aaGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg             >  1:8418280/1‑101 (MQ=255)
                                                                                            gATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc           >  2:5554921/1‑101 (MQ=255)
                                                                                             aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGTGTCGCCa          >  2:2201465/1‑101 (MQ=255)
                                                                                             aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa          >  2:8628076/1‑101 (MQ=255)
                                                                                             aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa          >  2:901848/1‑101 (MQ=255)
                                                                                             aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa          >  1:2907221/1‑101 (MQ=255)
                                                                                             aTAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCa          >  2:5831053/1‑101 (MQ=255)
                                                                                              tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg         >  2:5951553/1‑101 (MQ=255)
                                                                                              tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg         >  2:5363413/1‑101 (MQ=255)
                                                                                              tAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg         >  1:2036226/1‑101 (MQ=255)
                                                                                              gatgggggC.GTTTTTTTTTTTTT.TCCCCAAAAA.T.GCCGGGGGGGG..GGGGGGGC.GCCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc           <  2:1477740/97‑1 (MQ=255)
                                                                                               aaaGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc        >  2:7315059/1‑101 (MQ=255)
                                                                                                aaGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg       >  2:668987/1‑101 (MQ=255)
                                                                                                aaGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg       >  2:4139268/1‑101 (MQ=255)
                                                                                                 aGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTTACGCCGCGAACGTCTCGGTGGCAGCCAgcgg      >  2:6936794/1‑100 (MQ=18)
                                                                                                    ggC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac   >  2:767839/1‑101 (MQ=255)
                                                                                                     |                                                                                                             
TCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGC.TTTGCTTTTATCTT.TCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAG.AGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACG  >  NC_014034/3439694‑3439896

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG