Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,796 T→A 2.8% intergenic (‑99/+64) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7960T→A 2.8% 17.0 ‑4.1 ‑0.3355intergenic (‑99/+64)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (2/8):  ref base (213/131):  total (216/139)
Fisher's exact test strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.00e-01

Only 100 of 470 total aligned reads displayed.

ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898
                                                                                                    |                                                                                                       
aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgct                                                                                                         <  2:6113441/101‑1 (MQ=255)
 tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctt                                                                                                        <  1:3174106/101‑1 (MQ=255)
  ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATATTGGGTCAGCCAAATGGgcttt                                                                                                       >  2:1679041/1‑101 (MQ=255)
  ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                       <  2:273714/101‑1 (MQ=255)
   gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttg                                                                                                      <  2:3432869/101‑1 (MQ=255)
   gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttg                                                                                                      <  2:6898552/101‑1 (MQ=255)
    cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAAGGggttttg                                                                                                     >  1:5018241/1‑99 (MQ=38)
    cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCATTTAGGGCCAGAAAAAAGGgctttgg                                                                                                     >  1:7088689/1‑100 (MQ=255)
    cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgc                                                                                                     <  1:5343548/101‑1 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGATAAAGGGgctttgct                                                                                                    >  2:4393213/1‑101 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgct                                                                                                    <  2:1326587/101‑1 (MQ=255)
        gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                 <  1:7146853/101‑1 (MQ=255)
        gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                 <  1:554104/101‑1 (MQ=255)
        gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                 <  1:3638379/101‑1 (MQ=255)
        caCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGTCTGCGGCCGATGCCTGCGGCGGGGATATTTGGGGCAAGATAAAGGCgcttgtctgtt                                                                                                 <  2:5867572/100‑8 (MQ=38)
         aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTa                                                                                                <  2:6703929/101‑1 (MQ=255)
          cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                               <  2:5079062/101‑1 (MQ=255)
          cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                               <  1:2054791/101‑1 (MQ=255)
           ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGGTTTGCttttttt                                                                                              >  2:7470312/1‑98 (MQ=255)
            cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt                                                                                             <  2:2581257/101‑1 (MQ=255)
              acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCttt                                                                                           <  1:8643493/101‑1 (MQ=255)
               cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTc                                                                                          <  2:6309659/101‑1 (MQ=255)
               cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTc                                                                                          <  1:7910271/101‑1 (MQ=255)
                acGNCNCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTcc                                                                                         <  1:3854004/101‑1 (MQ=255)
                  ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTcccc                                                                                       <  1:1756317/101‑1 (MQ=255)
                   gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                      <  2:4326926/101‑1 (MQ=255)
                     gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAAGGGGTTTTCTTTTTTTTTTTCCCaaa                                                                                    >  2:5552733/1‑101 (MQ=37)
                     gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCaaa                                                                                    <  2:1532489/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGATAAAGGGGGTTTGCTTTTTTCTTTCCCCAAata                                                                                  >  1:8902952/1‑101 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata                                                                                  <  2:1806090/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata                                                                                  <  2:2858119/101‑1 (MQ=255)
                        aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                                 <  1:4110108/101‑1 (MQ=255)
                        aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                                 <  2:5813370/101‑1 (MQ=255)
                         ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.c                                                                               <  1:5080761/101‑1 (MQ=255)
                            gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCg                                                                            <  1:5781416/101‑1 (MQ=255)
                             cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGGTTTGGTTTTTTCTTTTCCCAAATAT.ccccg                                                                           >  1:680308/1‑99 (MQ=38)
                              tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAAGGGGTTTGCTTTTTTTTTTTCCCCAATAT.CCCcgg                                                                          >  1:4733915/1‑101 (MQ=37)
                               ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTTCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTCCTTTTATCTTTCCCCAAATAT.CCCgggg                                                                         <  1:8090007/101‑1 (MQ=255)
                                 cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGt                                                                       <  2:8370919/101‑1 (MQ=255)
                                  gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTg                                                                      <  1:629043/101‑1 (MQ=255)
                                   aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGc                                                                     <  1:4360606/101‑1 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCg                                                                    <  1:1355211/101‑1 (MQ=255)
                                    aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCg                                                                    <  2:5435723/101‑1 (MQ=255)
                                     aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGCTTTGCTTTTATTTTTCCCCCAAAAT.CCCGGGGGGGCgg                                                                   >  1:2269451/1‑101 (MQ=255)
                                      gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTGCTTTTATTTTTTCCCAAATAT.CCCCGGGGGGCggg                                                                  >  1:5873696/1‑101 (MQ=38)
                                      gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCggg                                                                  <  2:8667106/101‑1 (MQ=255)
                                        cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGCTTTTGTTTTTTTTTTCCCCCAAAATCCCCGGGGGGGCgggg                                                                 >  1:7864484/1‑101 (MQ=255)
                                         aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGATAAAAGGGGTTTGCTTTTTTTTTTTCCCAAAAAT.CCCCGGGGGGGgggggg                                                               >  1:9152790/1‑100 (MQ=25)
                                          ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCa                                                              <  2:7983248/101‑1 (MQ=255)
                                            aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCaga                                                            <  2:6562996/101‑1 (MQ=255)
                                            aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTGCCCCAAATAT.CCCGGGGGTGCGGGGGCaga                                                            <  2:3008037/101‑1 (MQ=255)
                                             aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGGTTTGCTTTTTTTTTTCCCCCAATAT.CCCGGGGGGGGGGGGGGagag                                                           >  1:6029699/1‑101 (MQ=255)
                                               tCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGATAAAGGGGGTTTGCTTTTATATTTCCCCAAATAT.TCCGGGGGGGCGGGGGGagaggc                                                         >  1:8421602/1‑99 (MQ=37)
                                               tCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGcc                                                         <  1:9009695/101‑1 (MQ=255)
                                                 ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTTCCCAAAAAT.CCCGGGGGGGCGGGGGCAGAGcccc                                                       >  2:2076442/1‑101 (MQ=25)
                                                  gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATAT.CCCGGGGGTGCGGGGGCAGAGccccc                                                      >  2:7110040/1‑101 (MQ=255)
                                                  gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGccccc                                                      <  1:1893941/101‑1 (MQ=255)
                                                     tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCCcgcc                                                   <  1:7007310/101‑2 (MQ=255)
                                                     tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCCGGGGGGCGGGGGCAGAGCCCCcgcc                                                   >  2:4489460/1‑100 (MQ=255)
                                                      gCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATAT.CCCCGGGGGGCGGGGGGAGAGCCCCcgccg                                                  >  1:3381442/1‑99 (MQ=21)
                                                        ggCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATAT.CCCGGGGGGGCGGGGGCAGAGCCCCcgccgcc                                                >  1:8241177/1‑97 (MQ=21)
                                                          ccGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCCcgccgcccc                                              >  2:8796533/1‑95 (MQ=255)
                                                            gATGCCTCCGGCGGGGCTACTTGGGGGTAGACAAATGGGCTTTTTTTCTTACTTTCCCCCAATAT.CCCTGGGGGGCGGGGGC.GGGCCCCC.CGCCGCCAAgg                                          >  2:3196534/1‑101 (MQ=255)
                                                             aTGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGGGCGGGGGCAGAGCCCCCGcccccacggt                                           >  2:8077167/1‑95 (MQ=18)
                                                                   ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgc                                   >  2:7862046/1‑101 (MQ=255)
                                                                       cggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                               >  2:4682804/1‑101 (MQ=255)
                                                                        ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                              >  1:1030055/1‑101 (MQ=255)
                                                                        ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                              >  1:4895523/1‑101 (MQ=255)
                                                                         gggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                             >  1:7427324/1‑101 (MQ=255)
                                                                         gggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                             >  2:1986238/1‑101 (MQ=255)
                                                                          ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                            >  1:124399/1‑101 (MQ=255)
                                                                           gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                           >  1:2505487/1‑101 (MQ=255)
                                                                           gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                           >  1:920765/1‑101 (MQ=255)
                                                                            atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                          >  1:7719459/1‑101 (MQ=255)
                                                                             tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                         >  2:3625453/1‑101 (MQ=255)
                                                                             tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                         >  1:459610/1‑101 (MQ=255)
                                                                              atTTGGGGCAAGCTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                        >  1:6568642/1‑101 (MQ=255)
                                                                                 tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                     >  2:3995226/1‑101 (MQ=255)
                                                                                  ggggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                    >  2:5645024/1‑101 (MQ=255)
                                                                                   gggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                   >  1:2207424/1‑101 (MQ=255)
                                                                                      cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                >  2:6940845/1‑101 (MQ=255)
                                                                                      cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                >  1:85075/1‑101 (MQ=255)
                                                                                       aaGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg               >  2:8267685/1‑101 (MQ=255)
                                                                                       aaGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCg               >  1:2969677/1‑101 (MQ=255)
                                                                                        aGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc              >  2:4890813/1‑101 (MQ=255)
                                                                                          aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            >  2:8628076/1‑101 (MQ=255)
                                                                                          aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            >  2:901848/1‑101 (MQ=255)
                                                                                          aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            >  2:3803922/1‑101 (MQ=255)
                                                                                           tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg           >  2:2531627/1‑101 (MQ=255)
                                                                                            aaaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  2:6439485/1‑101 (MQ=255)
                                                                                            aaaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  2:7315059/1‑101 (MQ=255)
                                                                                            aaaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  1:2846141/1‑101 (MQ=255)
                                                                                            aaaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  1:236217/1‑101 (MQ=255)
                                                                                             aaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg         >  2:1125362/1‑101 (MQ=255)
                                                                                             aaGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg         >  2:2240606/1‑101 (MQ=255)
                                                                                              aGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc        >  2:2340196/1‑101 (MQ=255)
                                                                                              aGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc        >  2:3304031/1‑101 (MQ=255)
                                                                                                gggCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga      >  2:4160766/1‑101 (MQ=255)
                                                                                                 ggCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac     >  2:767839/1‑101 (MQ=255)
                                                                                                   cttggctttTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTGCGGGGTCGCCAGCGcgacga   >  1:8804023/1‑101 (MQ=37)
                                                                                                    |                                                                                                       
ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATAT.CCCGGGGGTGCGGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG