Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,798 T→C 3.0% intergenic (‑101/+62) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7980T→C 3.0% 11.9 ‑3.6 ‑0.0355intergenic (‑101/+62)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (213/131):  total (216/139)
Fisher's exact test strand distribution p-value = 2.75e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 472 total aligned reads displayed.

GGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATAT  >  NC_014034/3439698‑3439900
                                                                                                     |                                                                                                         
ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgcttt                                                                                                           <  2:5777704/101‑1 (MQ=255)
ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgcttt                                                                                                           <  1:2813669/101‑1 (MQ=255)
 gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgctttg                                                                                                          <  2:6898552/101‑1 (MQ=255)
  cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgctttgc                                                                                                         <  1:716876/101‑1 (MQ=255)
  cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGgctttgc                                                                                                         <  1:4728859/101‑1 (MQ=255)
   ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGGAGAAAAAAGGGggtttgt                                                                                                         >  1:6343145/1‑100 (MQ=255)
      gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                     <  1:3638379/101‑1 (MQ=255)
        cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGAAGATAAAAGGGCTTTGCtttttt                                                                                                   >  1:5206304/1‑99 (MQ=255)
        cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                                   <  1:5001012/101‑1 (MQ=255)
         ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAAGGGGTTTGCttttttt                                                                                                  >  2:7470312/1‑98 (MQ=255)
          cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGATAAAGGGGGTTTGGtttttttt                                                                                                 >  2:6705313/1‑97 (MQ=38)
             cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTc                                                                                              <  2:6309659/101‑1 (MQ=255)
             cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTc                                                                                              <  1:2152482/101‑1 (MQ=255)
               cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTccc                                                                                            <  1:2331062/101‑1 (MQ=255)
                ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGA.TTTGGGGGAAGATAAAGGGGGTTTGGTTTTTTTTTTtccc                                                                                           >  1:8692457/1‑101 (MQ=255)
                ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTcccc                                                                                           <  1:1756317/101‑1 (MQ=255)
                 gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                          <  1:7729225/101‑1 (MQ=255)
                 gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCa                                                                                          <  1:373688/101‑1 (MQ=255)
                    ggtccAGCTTTCTAAAGACAGGAAGTCGGCCTGCGGCTGATTCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTCCTTTTATCTTTCCCCAAat                                                                                       <  2:4975315/98‑1 (MQ=37)
                    cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAat                                                                                       <  1:407693/101‑1 (MQ=255)
                      aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                                     <  2:5975025/101‑1 (MQ=255)
                      aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                                     <  1:7517738/101‑1 (MQ=255)
                      aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAatat                                                                                     <  2:8042504/101‑1 (MQ=255)
                       ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATc                                                                                    <  1:5897177/101‑1 (MQ=255)
                         aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAAGGGCTTTGCTTTTTTCTTTTCCCCAATATccc                                                                                  >  1:8302733/1‑101 (MQ=38)
                          gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCg                                                                                 <  2:1829659/101‑1 (MQ=255)
                          gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCg                                                                                 <  1:5478555/101‑1 (MQ=255)
                            tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATATCCCcgg                                                                               >  1:8989804/1‑101 (MQ=255)
                            tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCACATATCCCggg                                                                               >  2:5467548/1‑101 (MQ=255)
                             ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGGAAGATAAAGGGGGTTTGCTTTTTTTTTTCCCCCAATATCCCcggg                                                                              >  1:9192619/1‑101 (MQ=255)
                               cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGt                                                                            <  2:8370919/101‑1 (MQ=255)
                                gAAAGCCGGGAATTCGCCCTGGTGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTg                                                                           <  2:6939690/101‑1 (MQ=25)
                                gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTg                                                                           <  2:3737058/101‑1 (MQ=255)
                                 aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGc                                                                          <  2:1401541/101‑1 (MQ=255)
                                  aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..g                                                                       <  2:3473417/101‑1 (MQ=255)
                                  aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..g                                                                       <  1:1355211/101‑1 (MQ=255)
                                    gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTA.TTTTGGGCAAGATAAAGGGGCTTCTCTTTTTTCTTTTCCCAAAAATTCCCGGGGGGC..ggg                                                                     >  1:8181330/1‑101 (MQ=25)
                                    gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..ggg                                                                     <  2:8667106/101‑1 (MQ=255)
                                        ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCa                                                                 <  2:7983248/101‑1 (MQ=255)
                                         gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCag                                                                <  1:3138952/101‑1 (MQ=255)
                                          gaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCaga                                                               <  1:9044950/100‑1 (MQ=255)
                                          aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCaga                                                               <  2:6562996/101‑1 (MQ=255)
                                            ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc                                                             <  2:3070828/101‑1 (MQ=255)
                                             tCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTTGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCCGGGGTGC..GGGGGCCGAGc.c                                                           >  2:3880370/1‑101 (MQ=255)
                                             tCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.c                                                           <  1:4521801/101‑1 (MQ=255)
                                              cGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGGAAGATAAAAGGGGTTTGCTTTTATCTTTTCCCCAACATCCCGGGGGGGC..GGGGGCAGAGc.cc                                                          >  1:8023470/1‑101 (MQ=37)
                                               ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.ccc                                                         <  1:4810286/101‑1 (MQ=255)
                                               ggCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGc.ccc                                                         >  2:4266808/1‑101 (MQ=255)
                                                gCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATATCCCGGGGGTGC..GGGGGCAGAGc.cccc                                                        >  2:7110040/1‑101 (MQ=255)
                                                   tGCGGCCGATGCCTCCGGCGGGGGTA.TTTGGGGGAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcgcc                                                     >  2:2809324/1‑100 (MQ=255)
                                                    gCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATATCCCCGGGGGGC..GGGGGGAGAGC.CCCcgccg                                                    >  1:3381442/1‑99 (MQ=21)
                                                      tttcGATGCCTCCGGCGGGGGTA.TTTTGGGGCAGATGAA.AACCATCCCTTTCATCTTGCCCCAAATATCCCCGGGGGCC..CGGGGGGGAGCGCCCCCcgccc                                                  >  2:2041497/4‑100 (MQ=21)
                                                      tttcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.CCCCATCCCTTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcca                                                   <  2:3244208/98‑6 (MQ=21)
                                                        tcGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATGAA.AACCATCCCTTTCATCTTGCCCCAAATATCCCGGGGGGGCCGGGGGGCAGCGc.ccccccccccc                                                 >  2:286444/2‑94 (MQ=21)
                                                        ccGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcgccgcccc                                                >  2:8796533/1‑95 (MQ=255)
                                                          gATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTTCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGG.CCC..CGCCGCCACgg                                            >  2:2539998/1‑101 (MQ=18)
                                                           aTGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGt                                           >  2:1249704/1‑101 (MQ=255)
                                                                 ccGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCAcgc                                     >  1:7868494/1‑101 (MQ=255)
                                                                 ccGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCAcgc                                     >  2:7862046/1‑101 (MQ=255)
                                                                 ccGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCAcgc                                     >  2:235630/1‑101 (MQ=255)
                                                                  cggcggGGATA.TTTGGGGGAAGATAAAAGGGGTTTGCTTTTTTCTTTCCCCCAATATCCCGGGGGGGC..GGGGGGGGAGG.CCCC.C.CCGCCACGGGTCAcgcc                                    >  2:454752/1‑101 (MQ=19)
                                                                      ggggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                                >  1:6726246/1‑101 (MQ=255)
                                                                      ggggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGa                                >  1:1030055/1‑101 (MQ=255)
                                                                       gggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.GCC..CGCCGCCACGGTTCACGCCGCGAc                               >  1:615585/1‑101 (MQ=255)
                                                                       gggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                               >  1:855582/1‑101 (MQ=255)
                                                                        ggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                              >  2:2265549/1‑101 (MQ=255)
                                                                        ggATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                              >  1:124399/1‑101 (MQ=255)
                                                                         gATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCg                             >  2:1564350/1‑101 (MQ=255)
                                                                         gATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCg                             >  1:8471729/1‑101 (MQ=255)
                                                                          ata.tTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGt                            >  2:4130407/1‑101 (MQ=255)
                                                                          ata.tTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGt                            >  2:4447809/1‑101 (MQ=255)
                                                                           ta.tTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                           >  2:764979/1‑101 (MQ=255)
                                                                           ta.tTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                           >  2:1739455/1‑101 (MQ=255)
                                                                           ggttgtGGGGGAAAAGAGAGGGGTTTTTTTTTTTTTTCTCCCCCAAAAGCCCGGGGGGGG..GGGGGGGCAGC.CCCCCCGCCGCCACGGTTCACGCCGCGAcc                              <  1:5638753/96‑1 (MQ=255)
                                                                            tttgtGGGGGAAGGAAAGGGGGTTTTTTTTTTTTCTCCCCCCAAATGGCGGGGGGGGGG..GGGGGGCG.GC.CCCCCCGCCGCCACGGTTCACGCCGCGACCGt                            <  1:5827622/97‑1 (MQ=255)
                                                                                tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                       >  1:2240371/1‑101 (MQ=255)
                                                                                 ggggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGCCGCCgg                      >  1:2894670/1‑101 (MQ=21)
                                                                                  gggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                     >  1:7057766/1‑101 (MQ=255)
                                                                                     cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                  >  2:4764794/1‑101 (MQ=255)
                                                                                      aaGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                 >  2:8267685/1‑101 (MQ=255)
                                                                                       aGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                >  2:3338342/1‑101 (MQ=255)
                                                                                        gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc               >  2:2158342/1‑101 (MQ=255)
                                                                                        gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc               >  2:5554921/1‑101 (MQ=255)
                                                                                         aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa              >  2:8628076/1‑101 (MQ=255)
                                                                                         aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa              >  2:104727/1‑101 (MQ=255)
                                                                                         aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa              >  2:901848/1‑101 (MQ=255)
                                                                                         aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCa              >  2:5831053/1‑101 (MQ=255)
                                                                                          tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg             >  2:5363413/1‑101 (MQ=255)
                                                                                          tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg             >  1:2036226/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc            >  2:6439485/1‑101 (MQ=255)
                                                                                           aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc            >  2:7315059/1‑101 (MQ=255)
                                                                                               gggCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga        >  1:8863044/1‑101 (MQ=255)
                                                                                                 gctttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg      >  2:4334537/1‑101 (MQ=255)
                                                                                                 gctttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg      >  1:2427069/1‑101 (MQ=255)
                                                                                                   tttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat    >  1:6830989/1‑101 (MQ=255)
                                                                                                   tttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat    >  2:2626421/1‑101 (MQ=255)
                                                                                                    ttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata   >  1:5053402/1‑101 (MQ=255)
                                                                                                     tgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat  >  1:4289663/1‑101 (MQ=255)
                                                                                                     tgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat  >  1:1866418/1‑101 (MQ=255)
                                                                                                     tgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat  >  1:7089204/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
GGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATA.TTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATAT  >  NC_014034/3439698‑3439900

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG