Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,804 T→C 2.9% intergenic (‑107/+56) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8040T→C 2.9% 12.3 ‑3.9 ‑0.0376intergenic (‑107/+56)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (239/126):  total (242/134)
Fisher's exact test strand distribution p-value = 1.99e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 471 total aligned reads displayed.

GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906
                                                                                                    |                                                                                                         
gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                           <  2:2850977/101‑1 (MQ=255)
gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                           <  1:7146853/101‑1 (MQ=255)
 aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTa                                                                                                          <  1:4731489/101‑1 (MQ=255)
  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                                         <  1:5001012/101‑1 (MQ=255)
   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATc                                                                                                        >  1:3222310/1‑101 (MQ=255)
    cTAGACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGGAGATAAAAGGGGTTTTCtttttttt                                                                                                       >  1:8818633/1‑97 (MQ=255)
    cTACCCGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGAGGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt                                                                                                       <  1:8456197/101‑1 (MQ=255)
    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt                                                                                                       <  2:8344370/101‑1 (MQ=255)
     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCtt                                                                                                      <  1:236918/101‑1 (MQ=255)
         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTccc                                                                                                  <  1:8275740/101‑1 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGATAAAGGGGGTTTGGTTTTTTTTTTtccc                                                                                                 >  1:8692457/1‑101 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTcccc                                                                                                 <  1:5833657/101‑1 (MQ=255)
            cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCaa                                                                                               <  2:2525773/101‑1 (MQ=255)
              cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAat                                                                                             <  2:4577163/101‑1 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata                                                                                            <  1:4145803/101‑1 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata                                                                                            <  2:1806090/101‑1 (MQ=255)
                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCTATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCGTTCCCCAAata.t                                                                                          <  1:8172045/101‑1 (MQ=255)
                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata.t                                                                                          <  2:5975025/101‑1 (MQ=255)
                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAata.t                                                                                          <  1:7517738/101‑1 (MQ=255)
                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.Tc                                                                                         <  2:6712927/101‑1 (MQ=255)
                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.Tc                                                                                         <  1:5897177/101‑1 (MQ=255)
                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.Tc                                                                                         <  2:8640751/101‑1 (MQ=255)
                    gCTTTCGAAAGACAGGATTCCGCCCTGCGGCCGATCCCCCCGGGGGGGATTTTTGGGCAAAAATAAGGGGGTTTTGTTTTTTTCTTCCCCCAAATA.CCCCg                                                                                      <  2:3903873/101‑1 (MQ=255)
                     cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGGTTTGGTTTTTTCTTTTCCCAAATA.Tccccg                                                                                     >  1:680308/1‑99 (MQ=38)
                         cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGt                                                                                 <  1:1415404/101‑1 (MQ=255)
                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAAGGGCTTTGCTTTTTTCTTTTCCCCAATA.TCCCggggggg                                                                                >  2:2330136/1‑99 (MQ=37)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGGTTTACTTTTATCTTTTCCCAAATA.TCCCaggggcgg                                                                               >  2:3767652/1‑98 (MQ=255)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAGATAAAGGGGCTTTGCTTTTTTCTTTTCCCCAATATTCCCggggggc                                                                                >  2:8234110/1‑99 (MQ=37)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGGTTTGCTTTTATCTTTCCCCCAATA.TCCCGGGGGTGc                                                                               >  2:2211950/1‑101 (MQ=255)
                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAAATAAAAGGGGTTTGCTTTTTTCTTTCCCCCAATA.TCCCGGGGGTggg                                                                              >  1:700960/1‑99 (MQ=255)
                               accggAATTCGGCCTGCGGCCGGTGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..gggg                                                                         <  1:7748947/98‑1 (MQ=255)
                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGc                                                                       <  1:8542827/101‑1 (MQ=255)
                                  ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCa                                                                      <  2:7983248/101‑1 (MQ=255)
                                   gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCag                                                                     <  1:5723062/101‑1 (MQ=255)
                                    aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGCGGGGGC..GGGGGCgga                                                                    >  2:9019060/1‑101 (MQ=37)
                                    aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTGCCCCAAATA.TCCCGGGGGTGC..GGGGGCaga                                                                    <  2:3008037/101‑1 (MQ=255)
                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAGGGGGTTTGCTTTTATTTTTTCCCAAAAA.TCCCGGGGGTGC..GGGGGGAGGGcgc                                                                >  2:4483840/1‑99 (MQ=25)
                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGGGC..GGGGGCAGAGccc                                                                >  2:3558817/1‑101 (MQ=255)
                                         ggCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATA.TCCCGGGGGGGC..GGGGGGAGAGcccc                                                               >  2:4133114/1‑101 (MQ=25)
                                         ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTTCCCAAAAA.TCCCGGGGGGGC..GGGGGCAGAGcccc                                                               >  2:2076442/1‑101 (MQ=25)
                                         ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGcccc                                                               <  1:4810286/101‑1 (MQ=255)
                                         ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGGGC..GGGGGCAGAGcccc                                                               >  2:4266808/1‑101 (MQ=255)
                                           ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCGCCcg                                                             <  2:788429/101‑1 (MQ=255)
                                            tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTGCCCCAAATA.TCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                             <  2:257900/94‑2 (MQ=25)
                                            tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCTTGCCCCAAATA.TCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                             <  1:3982275/94‑2 (MQ=25)
                                             tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                           <  2:1672670/101‑2 (MQ=255)
                                                tttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.CCCCATCCCTTTCATCTTGCCCCAAATA.TCCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                                         <  2:3244208/98‑6 (MQ=21)
                                                   cGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGGCGAGCCCCcgccgccacg                                                     >  2:1898846/1‑94 (MQ=21)
                                                   cGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAAGGGCTTTGCTTTTTTCTTTCCCCCAATA.TCCCGGGGGGGG..GGGGGCAGAGCCCCCGcccccacg                                                     >  1:2325990/1‑97 (MQ=21)
                                                    gATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCCAATA.TCCCGGGGGTGC..GGGGGCAGAGGCCC..CGCCGCCACgg                                                  >  2:331536/1‑101 (MQ=18)
                                                    gATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                                  >  2:4856636/1‑101 (MQ=255)
                                                     aTGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGGGC..GGGGGCAGAGCCCCCGcccccacggt                                                   >  2:8077167/1‑95 (MQ=18)
                                                           ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgc                                           >  1:7868494/1‑101 (MQ=255)
                                                                ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                      >  1:1030055/1‑101 (MQ=255)
                                                                  ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                    >  1:124399/1‑101 (MQ=255)
                                                                    atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                  >  2:6552024/1‑101 (MQ=255)
                                                                    atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                  >  2:6227201/1‑101 (MQ=255)
                                                                    atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                  >  1:1963402/1‑101 (MQ=255)
                                                                     tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                 >  1:6247997/1‑101 (MQ=255)
                                                                      atTTGGGGCAAGCTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                >  1:6568642/1‑101 (MQ=255)
                                                                       tttGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                               >  1:222636/1‑101 (MQ=255)
                                                                        ttGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                              >  2:1262745/1‑101 (MQ=255)
                                                                         tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                             >  2:2704565/1‑101 (MQ=255)
                                                                         tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                             >  1:2693546/1‑101 (MQ=255)
                                                                         tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCAGCGACCGTCTCCg                             >  2:6917978/1‑101 (MQ=255)
                                                                           gggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                           >  1:5512016/1‑101 (MQ=255)
                                                                             gCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc                         >  2:4329804/1‑100 (MQ=255)
                                                                             gCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc                         >  2:8848744/1‑100 (MQ=255)
                                                                              cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                        >  2:7140683/1‑101 (MQ=255)
                                                                              cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                        >  1:1865527/1‑101 (MQ=255)
                                                                              cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.GCCCGGGGGAGC..GGGGGCAGAGCCCCCGC.CC.CCACGGGGCACCCCGCGCCAGTCGCCgtggcg                        >  2:8739548/1‑99 (MQ=9)
                                                                               aaGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                       >  1:342235/1‑101 (MQ=255)
                                                                               aaGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCg                       >  1:2969677/1‑101 (MQ=255)
                                                                                 gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  2:3119065/1‑101 (MQ=255)
                                                                                 gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  1:9031475/1‑101 (MQ=255)
                                                                                  aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  1:5075505/1‑101 (MQ=255)
                                                                                  aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  2:5503241/1‑101 (MQ=255)
                                                                                  aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCa                    >  2:5831053/1‑101 (MQ=255)
                                                                                   tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                   >  2:5363413/1‑101 (MQ=255)
                                                                                   tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                   >  1:3648570/1‑101 (MQ=255)
                                                                                   tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAg                   >  1:2130798/1‑101 (MQ=255)
                                                                                   gatgggggCGTTTTTTTTTTTTTTCCCCAAAAA.TGCCGGGGGGGG..GGGGGGGCGCCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     <  2:1477740/97‑1 (MQ=255)
                                                                                    aaaGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  2:7370234/1‑101 (MQ=255)
                                                                                    aaaGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  1:8988212/1‑101 (MQ=255)
                                                                                     aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                 >  2:668987/1‑101 (MQ=255)
                                                                                      aGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                >  2:7537450/1‑101 (MQ=255)
                                                                                      aGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                >  2:6971080/1‑101 (MQ=255)
                                                                                        gggCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga              >  1:4563049/1‑101 (MQ=255)
                                                                                         ggCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac             >  2:767839/1‑101 (MQ=255)
                                                                                             ttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata         >  1:98238/1‑101 (MQ=255)
                                                                                              tgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat        >  1:7089204/1‑101 (MQ=255)
                                                                                               gctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc       >  1:9167905/1‑101 (MQ=255)
                                                                                                ctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATcc      >  2:1118383/1‑101 (MQ=255)
                                                                                                 ttttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCca     >  1:4292433/1‑101 (MQ=255)
                                                                                                  tttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat    >  2:5553353/1‑101 (MQ=255)
                                                                                                  tttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat    >  2:5006680/1‑101 (MQ=255)
                                                                                                  tttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat    >  1:5247883/1‑101 (MQ=255)
                                                                                                   ttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc   >  2:6424311/1‑101 (MQ=255)
                                                                                                   ttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc   >  2:5736856/1‑101 (MQ=255)
                                                                                                    tATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca  >  1:8026803/1‑101 (MQ=255)
                                                                                                    |                                                                                                         
GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG