Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,392 G→C 95.6% intergenic (‑102/+28) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3920G→C95.6% 17.6 ‑4.3 ‑0.4271intergenic (‑102/+28)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (114/145):  ref base (10/2):  total (124/147)
Fisher's exact test strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.29e-01

Only 100 of 275 total aligned reads displayed.

TTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCG  >  NC_014034/3461305‑3461487
                                                                                        |                                                                                                
       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                         >  2:5050361/1‑101 (MQ=255)
        cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                        >  2:6826571/1‑101 (MQ=255)
        cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                        >  1:1907117/1‑101 (MQ=255)
        cGCAGCTTAGCAGGATGCACGGGCGCGCCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                        <  1:6107356/101‑1 (MQ=255)
        cGCACCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                        <  2:2899582/101‑1 (MQ=255)
         gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                       >  2:5900054/1‑101 (MQ=255)
         gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                       <  2:8255083/101‑1 (MQ=255)
         gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                       <  2:6009792/101‑1 (MQ=255)
          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCACAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCgc                                                                      >  2:4427419/1‑100 (MQ=255)
           aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                     >  1:7577371/1‑101 (MQ=255)
            gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                    <  1:2199735/101‑1 (MQ=255)
             cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                                                   >  2:3703660/1‑101 (MQ=255)
             cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                                                   <  2:1822847/101‑1 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                 >  2:6101027/1‑101 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                 >  2:422559/1‑101 (MQ=255)
               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                 >  1:3190473/1‑101 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                <  2:4673048/101‑1 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                >  2:5212379/1‑101 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                <  1:7550101/101‑1 (MQ=255)
                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                >  1:6748939/1‑101 (MQ=255)
                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                              >  2:5093187/1‑101 (MQ=255)
                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                              <  1:4124740/101‑1 (MQ=255)
                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                              <  2:447614/101‑1 (MQ=255)
                   aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                             >  2:7096206/1‑101 (MQ=255)
                   aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                             >  2:8511362/1‑101 (MQ=255)
                   aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                             >  1:1168453/1‑101 (MQ=255)
                     gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                           <  1:4203383/101‑1 (MQ=255)
                     gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                           >  1:6857039/1‑101 (MQ=255)
                      aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                          >  2:2491845/1‑101 (MQ=255)
                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                         <  2:3162842/101‑1 (MQ=255)
                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                       <  1:2866679/101‑1 (MQ=255)
                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                       >  2:8192962/1‑101 (MQ=255)
                          aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                      >  1:5059079/1‑101 (MQ=255)
                           cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacgc                                                     >  2:6924795/1‑100 (MQ=255)
                           cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacga                                                     >  1:6602408/1‑101 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    >  2:3563209/1‑101 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    <  1:5503447/101‑1 (MQ=255)
                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    >  1:5406514/1‑101 (MQ=255)
                             ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                   <  1:6601077/101‑1 (MQ=255)
                             ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                   <  1:8057732/101‑1 (MQ=255)
                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                  <  2:6398441/101‑1 (MQ=255)
                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                  <  2:8801573/101‑1 (MQ=255)
                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                  >  2:5247108/1‑101 (MQ=255)
                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCAAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                  >  1:8121024/1‑101 (MQ=255)
                               cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                 <  2:3280771/101‑1 (MQ=255)
                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                              <  2:3842948/101‑1 (MQ=255)
                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                              >  1:3474999/1‑101 (MQ=255)
                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                              >  2:8900716/1‑101 (MQ=255)
                                   gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                             >  1:4057839/1‑101 (MQ=255)
                                   gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                             <  1:2742773/101‑1 (MQ=255)
                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.TCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                            <  1:4285643/101‑1 (MQ=255)
                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                            <  1:8656277/101‑1 (MQ=255)
                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                           >  1:2601103/1‑101 (MQ=255)
                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                           <  2:9178707/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          >  2:2830442/1‑101 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          >  2:1699184/1‑101 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          >  1:4463454/1‑101 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          >  1:8447695/1‑101 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          <  1:826143/101‑1 (MQ=255)
                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACCATGACGTCagag                                          <  2:6692851/101‑1 (MQ=255)
                                          gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCGG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                      >  1:4777249/1‑101 (MQ=255)
                                           cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                     <  1:7828038/101‑1 (MQ=255)
                                            aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                    <  1:1393903/101‑1 (MQ=255)
                                            aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                    >  2:1207450/1‑101 (MQ=255)
                                             gTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                   <  1:3776169/101‑1 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                  >  2:562902/1‑101 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                  <  1:6833656/101‑1 (MQ=255)
                                              tttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGCTCTTGCCGACGATGACGCCAGAGGCCTtgat                                  >  1:1030616/1‑101 (MQ=255)
                                               ttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg                                 <  1:2582393/101‑1 (MQ=255)
                                                tGGCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                >  1:1319561/1‑101 (MQ=255)
                                                tGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                <  1:8758989/101‑1 (MQ=255)
                                                 ggCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                               >  1:9121454/1‑101 (MQ=255)
                                                 ggCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                               <  1:1750892/101‑1 (MQ=255)
                                                  gcgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                              <  2:242597/101‑1 (MQ=255)
                                                  gcgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                              <  1:2477706/101‑1 (MQ=255)
                                                  gcgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                              <  1:5104314/101‑1 (MQ=255)
                                                   cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                             <  2:2147142/101‑1 (MQ=255)
                                                   cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCAGACGATGACGTCAGAGGCCTTGATGATcg                             <  1:299108/101‑1 (MQ=255)
                                                    gcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                            <  2:7654011/101‑1 (MQ=255)
                                                                         gggggcgcagccACCCGGCCGCGCCCCCAACGCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc             >  2:1648941/11‑101 (MQ=255)
                                                                          ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg            <  2:275801/92‑1 (MQ=255)
                                                                          ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg            >  1:6920054/10‑101 (MQ=255)
                                                                            ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc          >  1:612014/8‑101 (MQ=255)
                                                                            ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc          <  2:4057724/94‑1 (MQ=255)
                                                                              cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt        >  2:8720463/6‑101 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       >  1:2361834/5‑101 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       >  2:8337685/5‑101 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       <  2:7542306/97‑1 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       <  1:5151441/97‑1 (MQ=255)
                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       <  1:6650587/97‑1 (MQ=255)
                                                                               ccagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       <  2:2257887/97‑1 (MQ=255)
                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc      <  2:5397229/98‑1 (MQ=255)
                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc      >  1:8518880/4‑101 (MQ=255)
                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc      <  2:2679153/98‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     >  2:7411393/3‑101 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     <  1:7100390/99‑1 (MQ=255)
                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCATTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     >  2:2219636/3‑101 (MQ=255)
                                                                                  gccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAg    >  2:725593/2‑101 (MQ=255)
                                                                                   ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc   <  1:5673260/101‑1 (MQ=255)
                                                                                    cACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgcg  >  1:4601560/1‑101 (MQ=255)
                                                                                        |                                                                                                
TTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCG  >  NC_014034/3461305‑3461487

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG