Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,653 T→C 100% F427L (TTC→CTC)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6530T→C100.0% 1591.5 433F427L (TTC→CTC) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (225/208):  ref base (0/0):  total (225/208)

Only 100 of 450 total aligned reads displayed.

AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCA  >  NC_014034/3531555‑3531750
                                                                                                  |                                                                                                 
aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                 >  2:3964910/1‑101 (MQ=255)
aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                 <  1:730369/101‑1 (MQ=255)
aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                 >  1:3280026/1‑101 (MQ=255)
aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                 <  1:722259/101‑1 (MQ=255)
 gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                                                                                <  1:8973987/101‑1 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                              >  1:5488164/1‑101 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                              >  2:5219935/1‑101 (MQ=255)
         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACATGCCCCTCGGCCGCGaa                                                                                        <  1:1905949/101‑1 (MQ=255)
         gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                                                                        >  1:1654679/1‑101 (MQ=255)
              cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                                                                   >  2:1453618/1‑101 (MQ=255)
               aGTGGCTGGCCGGGGGCCCGAACACCGAAGCCCCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                                                                  <  1:3089635/101‑1 (MQ=255)
               aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCgcg                                                                                  >  2:8278637/1‑99 (MQ=255)
                gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                                                                 >  2:1797493/1‑101 (MQ=255)
                  ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcc                                                                               <  1:6229817/101‑2 (MQ=255)
                  ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcc                                                                               >  1:6038137/1‑100 (MQ=255)
                  ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcc                                                                               >  1:7466445/1‑100 (MQ=255)
                   gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                                                                              >  1:2634935/1‑99 (MQ=255)
                   gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                                                                              <  2:455392/101‑3 (MQ=255)
                    ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                             <  1:4596317/101‑1 (MQ=255)
                    ccggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                             <  2:7794494/99‑1 (MQ=255)
                     tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                            >  2:8985353/1‑101 (MQ=255)
                     tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                            >  1:3437744/1‑101 (MQ=255)
                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                           <  2:2683080/101‑1 (MQ=255)
                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                           >  2:7663656/1‑101 (MQ=255)
                       gcCGAGGGCACGCTCACCGAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                                                                          <  2:2959390/101‑1 (MQ=255)
                        ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                                                                         >  2:5419360/1‑101 (MQ=255)
                        ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                                                                         <  1:9191476/101‑1 (MQ=255)
                         cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                        <  1:5569767/101‑1 (MQ=255)
                          gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                       >  2:7256204/1‑101 (MQ=255)
                           aGGGCCCGATGACCGAAGACGCCTAGCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                      <  2:6921139/101‑1 (MQ=255)
                           aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                      >  2:6275453/1‑101 (MQ=255)
                              gcaggctggccGAGGACGCCTATGCGAACGGGACAAAGATAATAACGCCCGACTATGACGGCCCGCCCCTGGGCCGCGAAGCCGATGCCTGGCTGAGGGcc                                                                   <  2:1516542/92‑1 (MQ=255)
                              gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                                                                   >  2:4885025/1‑101 (MQ=255)
                              gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                                                                   >  2:6780866/1‑101 (MQ=255)
                                 cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc                                                                <  2:3624144/101‑1 (MQ=255)
                                 cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc                                                                <  1:2345337/101‑1 (MQ=255)
                                  gATCCCCGAAGACTCCTATCCAAACGGCCCCGAGCTGATGAAGCGCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                               <  1:8277102/101‑1 (MQ=255)
                                  gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                               <  2:6178922/101‑1 (MQ=255)
                                  gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                               <  2:6460056/101‑1 (MQ=255)
                                    tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                             <  2:584123/101‑1 (MQ=255)
                                      ctcGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                           <  1:4085183/99‑1 (MQ=255)
                                      cccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                           <  1:2269306/100‑1 (MQ=255)
                                      aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                           <  2:3298203/101‑1 (MQ=255)
                                       ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTAGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                          <  1:11822/101‑1 (MQ=255)
                                        cGAAGACGCCTACCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg                                                         <  2:7469327/101‑1 (MQ=255)
                                         gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                        >  2:4435044/1‑101 (MQ=255)
                                          aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                       >  1:1564480/1‑101 (MQ=255)
                                           cgatctctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                      <  1:8052735/94‑1 (MQ=255)
                                            gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                     >  1:7042237/1‑101 (MQ=255)
                                            gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                     >  1:774600/1‑101 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                    >  1:1858015/1‑101 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGACCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                    <  2:5011643/101‑1 (MQ=255)
                                              cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                   <  2:577517/101‑1 (MQ=255)
                                               cccaaTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgc                                                  <  2:2394374/97‑1 (MQ=255)
                                                cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                 >  1:4532683/3‑101 (MQ=255)
                                                 ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgagaagatcg                                                >  2:8052395/2‑95 (MQ=255)
                                                 ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                >  1:3591135/2‑101 (MQ=255)
                                                 ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                <  1:8424819/100‑1 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                               <  2:5652604/101‑1 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                               >  1:8750966/1‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACAATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                               >  1:6453962/1‑101 (MQ=255)
                                                        aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                         >  2:7976190/1‑101 (MQ=255)
                                                           gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                      <  2:1121063/101‑1 (MQ=255)
                                                           gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                      >  1:3845414/1‑101 (MQ=255)
                                                               cggagtggaagaAGCCCGAAGATGGCACCACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtg                                  <  1:4408133/91‑1 (MQ=255)
                                                                cGAGCTGATGAAGCCCGACTATGACGACACTCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                 >  2:1233913/1‑101 (MQ=255)
                                                                cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                 >  1:579000/1‑101 (MQ=255)
                                                                 gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                >  1:247559/1‑101 (MQ=255)
                                                                 gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                <  2:8533565/101‑1 (MQ=255)
                                                                   gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTcc                              <  1:5918990/101‑1 (MQ=255)
                                                                   gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTcc                              <  1:215562/101‑1 (MQ=255)
                                                                     tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCcac                            >  1:6733856/1‑101 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                           >  2:4184849/1‑101 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                           >  2:379527/1‑101 (MQ=255)
                                                                       atgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGACTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACaa                          <  1:3934218/101‑1 (MQ=255)
                                                                        tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                         >  2:6137182/1‑101 (MQ=255)
                                                                         gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                        >  1:4282531/1‑101 (MQ=255)
                                                                         gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGAGTTCCACAAcc                        >  1:1410117/1‑101 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                       >  1:4643635/1‑101 (MQ=255)
                                                                           tccgatcttATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                      <  1:7940952/93‑1 (MQ=255)
                                                                            gCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGa                     >  1:8557168/1‑101 (MQ=255)
                                                                              ccGACTATGACGACCCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                   <  2:1654632/101‑1 (MQ=255)
                                                                              ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGTCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGAGGTCCACAACCTGATc                   >  2:2332918/1‑101 (MQ=255)
                                                                               cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCa                  >  1:1279169/1‑101 (MQ=255)
                                                                                gACTATGCCGACACGCCCCCCGGCCGGGACGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                 <  2:6574389/101‑1 (MQ=255)
                                                                                 aCTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAcc                >  2:9075407/1‑101 (MQ=255)
                                                                                 aCTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAcc                <  1:6938974/101‑1 (MQ=255)
                                                                                  cTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAccc               <  2:6045857/101‑1 (MQ=255)
                                                                                   tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt              >  2:871041/1‑101 (MQ=255)
                                                                                   tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGACTTCCAGACCGAGATCGCCACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt              >  1:6866268/1‑101 (MQ=255)
                                                                                    aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg             <  1:8916686/101‑1 (MQ=255)
                                                                                     tGACGCCACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc            <  2:473087/101‑1 (MQ=255)
                                                                                       acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc          >  2:2012655/1‑101 (MQ=255)
                                                                                       acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc          <  1:2783074/101‑1 (MQ=255)
                                                                                       acgacACGCCCCACGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc          >  1:6628781/1‑101 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa         <  1:4936164/101‑1 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa         <  1:1084165/101‑1 (MQ=255)
                                                                                             cGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTc    <  2:5506579/101‑1 (MQ=255)
                                                                                              gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc   >  1:911321/1‑101 (MQ=255)
                                                                                              gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc   <  1:5670451/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCA  >  NC_014034/3531555‑3531750

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG