Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,539,498 T→G 100% D261E (GAT→GAG prpD → 2‑methylcitrate dehydratase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,539,4980T→G100.0% 1864.1 505D261E (GAT→GAGprpD2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  new base (239/266):  ref base (0/0):  total (239/266)

Only 100 of 548 total aligned reads displayed.

ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595
                                                                                                  |                                                                                                 
aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgc                                                                                                 <  1:4120043/101‑1 (MQ=255)
aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgc                                                                                                 >  2:2497437/1‑101 (MQ=255)
   gAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGcgcg                                                                                              >  2:1939025/1‑101 (MQ=255)
    aGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGt                                                                                             >  2:207749/1‑101 (MQ=255)
      gCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTa                                                                                           <  2:1235284/101‑1 (MQ=255)
          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       <  1:4568929/101‑1 (MQ=255)
           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCg                                                                                      <  1:4650060/101‑1 (MQ=255)
             aCCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTCCACCGTc                                                                                    >  2:2746552/1‑101 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:2451792/101‑1 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  2:808324/101‑1 (MQ=255)
                 cccccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGCCGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGcc                                                                                <  1:7496630/99‑1 (MQ=255)
                      tGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGccc                                                                           >  1:3349829/1‑101 (MQ=255)
                       gcgcGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCg                                                                          >  2:5736556/1‑101 (MQ=255)
                          cggcgNAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       <  1:2989047/101‑1 (MQ=255)
                          cggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       >  2:7571739/1‑101 (MQ=255)
                          cggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       <  2:8574086/101‑1 (MQ=255)
                          cggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       >  1:828966/1‑101 (MQ=255)
                           ggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCa                                                                      >  1:7407356/1‑101 (MQ=255)
                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     <  1:207752/101‑1 (MQ=255)
                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     >  2:266460/1‑101 (MQ=255)
                             cgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGCCGCGTTACACCGTCCGCCTGCCCGGCCATg                                                                    >  2:2845785/1‑101 (MQ=255)
                              ggaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   <  1:810944/99‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAGGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  1:1170563/101‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  >  1:4791349/1‑101 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  2:831025/101‑1 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 <  2:7832474/101‑1 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 >  1:8582830/1‑101 (MQ=255)
                                    gcCCCCAGCCCCGCCTGGGAGGGGTAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGcc                                                             <  2:7159640/101‑1 (MQ=255)
                                    gcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGcc                                                             <  2:4589170/101‑1 (MQ=255)
                                      ccccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGa                                                           >  1:846481/1‑101 (MQ=255)
                                       cccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaa                                                          >  1:2675242/1‑101 (MQ=255)
                                        ccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaaa                                                         <  1:5679445/101‑1 (MQ=255)
                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  1:5556719/101‑1 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  2:3491296/1‑101 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  1:6204828/1‑101 (MQ=255)
                                           gCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcgc                                                      <  2:930463/101‑1 (MQ=255)
                                              ccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGcc                                                   <  2:4018931/101‑1 (MQ=255)
                                               cGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGCTGCTCTCGGGCCCAGAGGCGCGTTGCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCa                                                  >  1:4026315/1‑101 (MQ=255)
                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  2:3209756/101‑1 (MQ=255)
                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  2:5566133/101‑1 (MQ=255)
                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  1:9146749/101‑1 (MQ=255)
                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  1:3469805/101‑1 (MQ=255)
                                                gCCGGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 <  1:3209023/101‑1 (MQ=255)
                                                 cgtGGGAGGGGGAAGCCGGTTTCATCGCCGGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATc                                                <  1:2216992/99‑1 (MQ=255)
                                                 ccTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATc                                                <  1:1508532/101‑1 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  2:1747526/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  2:691236/1‑101 (MQ=255)
                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  1:5037990/101‑1 (MQ=255)
                                                       ggggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGac                                          <  2:8223301/100‑1 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  2:4976983/101‑1 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         >  1:2796499/1‑101 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  1:6476898/101‑1 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  1:7921553/101‑1 (MQ=255)
                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGacaa                                        >  2:3075934/1‑100 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     >  2:1069685/1‑101 (MQ=255)
                                                             aggACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  2:618001/99‑1 (MQ=255)
                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    >  1:5386256/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   >  2:8793723/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   <  1:8679557/101‑1 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   <  1:428399/101‑1 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGACGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   <  1:7203550/101‑1 (MQ=255)
                                                               gACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  1:234292/101‑1 (MQ=255)
                                                                aCGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 >  1:7986023/1‑101 (MQ=255)
                                                                   gTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAg                              >  1:2741851/1‑101 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGa                            >  2:2316450/1‑101 (MQ=255)
                                                                      tCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  2:4486632/101‑1 (MQ=255)
                                                                       cATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCAGCCTGCCGGGCCATGGCGAGCCGGAAGGGGCCATCCTTGCCACCTATAACACGCAat                          >  2:4237469/1‑101 (MQ=255)
                                                                        aTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         >  1:3665159/1‑101 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  2:6357638/101‑1 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  1:7572063/101‑1 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  2:521217/101‑1 (MQ=255)
                                                                          cGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATAtt                       <  1:5996368/101‑1 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  2:7266837/101‑1 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  2:7987505/101‑1 (MQ=255)
                                                                               ggATGCTCTCGGGCCCAGAGGCGCGTTACAACGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCCTGAGACCTATACCAAGGAATATTcggcg                  >  2:8997368/1‑101 (MQ=255)
                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 <  1:1381109/101‑1 (MQ=255)
                                                                                 aTGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGa                >  1:8787692/1‑101 (MQ=255)
                                                                                   gCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAat              >  1:6324510/1‑101 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             <  1:1581362/101‑1 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             <  2:7731027/101‑1 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             >  1:764122/1‑101 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  1:8879352/101‑1 (MQ=255)
                                                                                     cctcGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  1:1026031/100‑1 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACGTATACCAAGGAATATTCGGCGGaaaagc           >  2:133431/1‑97 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           <  1:6846027/101‑1 (MQ=255)
                                                                                      cgcGGGCCCAGAGGCGCGTTACGCCGCCCGCCTGCCCGGCCAGGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATGCGGCGGAATATc           <  1:8149836/99‑1 (MQ=255)
                                                                                       tcGGGCCCGGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          <  2:6652570/101‑1 (MQ=255)
                                                                                       tcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          <  2:5976301/101‑1 (MQ=255)
                                                                                       ccGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          <  2:4776957/100‑1 (MQ=255)
                                                                                        cGGGCCAAGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  2:2226231/101‑1 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        <  2:1583271/101‑1 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        <  1:1974387/101‑1 (MQ=255)
                                                                                          ggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaac       >  2:872761/1‑100 (MQ=255)
                                                                                            cccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     >  1:4377211/1‑101 (MQ=255)
                                                                                             ccAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    <  2:846610/101‑1 (MQ=255)
                                                                                             caaGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    <  2:3797647/99‑1 (MQ=255)
                                                                                              cAGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   <  1:3844988/101‑1 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  1:5847603/1‑101 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  1:5223495/1‑101 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  1:970166/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG