Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,541,575 G→C 100% S53S (TCG→TCC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,541,5750G→C100.0% 1614.5 428S53S (TCG→TCCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (203/225):  ref base (0/0):  total (203/225)

Only 100 of 441 total aligned reads displayed.

GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672
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gccCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCcgg                                                                                                 <  1:4571136/101‑1 (MQ=255)
  ccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGgcg                                                                                               >  1:3092464/1‑101 (MQ=255)
   cTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgcgc                                                                                              >  1:8867241/1‑101 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             <  2:5064606/101‑1 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             >  2:2142837/1‑101 (MQ=255)
       cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          >  1:8479588/1‑101 (MQ=255)
         cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        >  2:8594714/1‑101 (MQ=255)
         cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        >  1:1018613/1‑101 (MQ=255)
         cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCCGGCGGCTTTTCTGGCCGgcggcgccgg                                                                                        >  1:8945741/1‑94 (MQ=255)
          ggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATg                                                                                       <  1:3200415/101‑1 (MQ=255)
           gcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                      <  1:442399/101‑1 (MQ=255)
            cccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTc                                                                                     <  1:5315583/101‑1 (MQ=255)
            cccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTc                                                                                     <  1:334559/101‑1 (MQ=255)
             ccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCg                                                                                    >  2:5988500/1‑101 (MQ=255)
                gcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGcc                                                                                 >  2:6082396/1‑101 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               >  2:4050757/1‑101 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               <  2:7578067/101‑1 (MQ=255)
                     ctccgcCTGCCGGGCGGGCTTAACGGCATGGCCTATCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            <  1:2211794/98‑1 (MQ=255)
                     cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            >  1:1773755/1‑101 (MQ=255)
                     cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            >  2:5714579/1‑101 (MQ=255)
                           cTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCt                                                                      >  1:7694742/1‑101 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     <  1:1610962/101‑1 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     <  1:6001233/101‑1 (MQ=255)
                             gCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGc                                                                    <  2:1236401/101‑1 (MQ=255)
                              ccGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGcc                                                                   <  1:7914143/101‑1 (MQ=255)
                              ccGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGcc                                                                   >  1:3684178/1‑101 (MQ=255)
                               cGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCg                                                                  >  1:2439757/1‑101 (MQ=255)
                                  gcgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAc                                                               >  1:2777830/1‑101 (MQ=255)
                                    gcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCt                                                             <  1:7955701/101‑1 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  2:7896562/101‑1 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  2:1774159/101‑1 (MQ=255)
                                      gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                           >  2:8314167/1‑101 (MQ=255)
                                      gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                           <  2:1393308/101‑1 (MQ=255)
                                       cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                          <  1:1517931/101‑1 (MQ=255)
                                       cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                          >  2:7044690/1‑101 (MQ=255)
                                        aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGc                                                         <  2:6779106/101‑1 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       <  2:1871182/101‑1 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       <  2:2757954/101‑1 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       <  1:3767908/101‑1 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      <  2:908652/101‑1 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      >  2:3612809/1‑101 (MQ=255)
                                            cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                     >  2:250385/1‑101 (MQ=255)
                                              gCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAGCTCGGCATCCTg                                                   <  1:6678092/101‑1 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                  >  2:2590237/1‑101 (MQ=255)
                                                aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                                 <  2:3484417/101‑1 (MQ=255)
                                                aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                                 <  1:9009022/101‑1 (MQ=255)
                                                aTGGCCGCGCTGCACGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                                 >  1:939366/1‑101 (MQ=255)
                                                     cgcgCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTgg                                            <  1:5658297/101‑1 (MQ=255)
                                                      gcgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGa                                           <  1:4067807/101‑1 (MQ=255)
                                                       cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                          <  1:2274648/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  1:7346917/1‑101 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         <  2:7153403/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         <  1:4020316/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         <  1:64991/101‑1 (MQ=255)
                                                         cTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGa                                        >  2:3790968/1‑101 (MQ=255)
                                                          tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       >  1:5490522/1‑101 (MQ=255)
                                                          tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       >  2:801069/1‑101 (MQ=255)
                                                           gCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAgg                                      <  1:4636603/101‑1 (MQ=255)
                                                            cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGt                                     <  2:1142430/101‑1 (MQ=255)
                                                            cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGt                                     >  1:7072920/1‑101 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  1:2590319/101‑1 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    >  1:3897842/1‑101 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  2:5835886/101‑1 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  2:5213223/1‑101 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  2:745248/1‑101 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  2:6656190/1‑101 (MQ=255)
                                                                   aaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttt                              <  2:4393029/101‑1 (MQ=255)
                                                                    aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                             <  1:3455074/101‑1 (MQ=255)
                                                                    aGCCGCGGGGTTTCAGGCGCTTTATCTGGCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                             <  1:352229/101‑1 (MQ=255)
                                                                     gCCGCTGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttttt                            <  2:8945320/101‑1 (MQ=255)
                                                                     gCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttttt                            <  2:4910867/101‑1 (MQ=255)
                                                                      ccGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                           <  1:6085158/101‑1 (MQ=255)
                                                                         cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                        <  2:5541486/101‑1 (MQ=255)
                                                                         cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                        >  1:715500/1‑101 (MQ=255)
                                                                          ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  1:2945725/101‑1 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      <  1:6140127/101‑1 (MQ=255)
                                                                            ggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGc                     <  2:2880130/101‑1 (MQ=255)
                                                                             gTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGGCTTTTTCATCCGcc                    >  2:4109365/1‑101 (MQ=255)
                                                                             gTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                    >  1:6292634/1‑101 (MQ=255)
                                                                             gTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                    <  1:6741579/101‑1 (MQ=255)
                                                                             gTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                    >  2:4692876/1‑101 (MQ=255)
                                                                              tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   >  2:511189/1‑101 (MQ=255)
                                                                               ttaaGGCGTTTTATCTGTCCGGCTCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  <  1:1111626/98‑1 (MQ=255)
                                                                               ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  <  1:5028136/101‑1 (MQ=255)
                                                                                tCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGa                 <  1:8048812/101‑1 (MQ=255)
                                                                                 cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGac                >  2:2222138/1‑100 (MQ=255)
                                                                                 cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                <  2:6377542/101‑1 (MQ=255)
                                                                                  aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               >  1:5108988/1‑101 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  1:417389/101‑1 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  1:7520005/101‑1 (MQ=255)
                                                                                      gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc           >  1:1209356/1‑101 (MQ=255)
                                                                                        tttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCgg         <  2:7262451/101‑1 (MQ=255)
                                                                                          tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       >  2:8082240/1‑101 (MQ=255)
                                                                                          tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       >  2:3426239/1‑101 (MQ=255)
                                                                                           aTCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGcc      <  2:540543/101‑1 (MQ=255)
                                                                                           aTCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGCCGCCTTTTTCATCCGCCAGATCACACGGGcc      >  2:3180225/1‑101 (MQ=255)
                                                                                             cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc    <  1:8314530/101‑1 (MQ=255)
                                                                                             cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc    <  2:2346680/101‑1 (MQ=255)
                                                                                              tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   <  1:2068650/101‑1 (MQ=255)
                                                                                               gTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCgg  >  1:6836857/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG