BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	580,961	C→T	1.1%	intergenic (+82/‑289)	RCAP_rcc00541 → / → RCAP_rcc00542	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	580,961	0	C→T	1.1%	2.0 0.0 ‑0.0	463	intergenic (+82/‑289)	RCAP_rcc00541/RCAP_rcc00542	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/3): ref base (220/232): total (228/235)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.99e-01

Only 100 of 495 total aligned reads displayed.


GTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAT  >  NC_014034/580864‑581061


 ttCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGcc                                                                                                   >  1:7813838/1‑101 (MQ=255)


  tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                                  <  2:6012635/101‑1 (MQ=255)


    cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                                <  1:733836/101‑1 (MQ=255)


    cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                                <  1:2438986/101‑1 (MQ=255)


     gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                               <  1:8511628/101‑1 (MQ=255)


       cTCGATTGAGGCGGCCCGGTCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCAc                                                                                             >  1:4055941/1‑101 (MQ=255)


         cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATAACCt                                                                                           >  2:3474368/1‑101 (MQ=255)


          gATTGAGGCGGCCCGGGCAGGGGGGGCTGCCCCCCCTTTCGGGTGGGCCGGAATTCCCCCCCGGGATATTTGGGGGAAGATAAACGGGCGCCTATCAccct                                                                                          >  1:6478245/1‑99 (MQ=21)


          gATTGAGGCGGCCCGGGCAGGGGGCGGTGCCCCCTCTTTCCGCTGCGCCGAAATTTACCCCCGGGGTATTTTAGGGAAGATAAACGGGCGcccctccccct                                                                                          >  1:3109863/1‑92 (MQ=255)


          gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGCAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATCACCtt                                                                                          >  2:7675741/1‑101 (MQ=25)


          gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                                          <  2:5601394/101‑1 (MQ=255)


          gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCCAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                                          >  1:461675/1‑101 (MQ=255)


           aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTg                                                                                         <  1:2003278/101‑1 (MQ=255)


           aTTGAGGCGGCCCGGGCAGGCGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGTGCGCCTATCCCCTTg                                                                                         <  1:8833386/101‑1 (MQ=21)


            ttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCGCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTgg                                                                                        <  1:359935/101‑1 (MQ=255)


             tGAGGCGGCCCGGGCAGGGGGCGCTGCCCACTCTTTTGGCTGGGCCGAAATTGACACCCGGGAGATTTGAGGCAAGCTAAAAGGGCGCGTATAACCTTggg                                                                                       >  1:8937888/1‑100 (MQ=255)


               aGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGc                                                                                     >  1:9068557/1‑101 (MQ=255)


                ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCa                                                                                    <  1:8612750/101‑1 (MQ=255)


                 gcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaa                                                                                   <  2:3649040/101‑1 (MQ=255)


                  cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                                                  <  2:2762040/101‑1 (MQ=255)


                   ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGGGCCGGAATTCACCCCCGGGGTATTTTAGGCAAGATAAACGGGGGCCTATCACCTTGGTGCaaaa                                                                                 >  1:2960839/1‑100 (MQ=21)


                   ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                                                 <  2:6815863/101‑1 (MQ=255)


                    gcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAata                                                                                >  2:1075870/1‑101 (MQ=255)


                        gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                            <  1:8103137/101‑1 (MQ=255)


                        gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                            <  2:1398/101‑1 (MQ=255)


                        gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                            >  2:6960814/1‑101 (MQ=255)


                         ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                           >  1:2717381/1‑101 (MQ=255)


                          gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggg                                                                          <  2:4818206/101‑1 (MQ=255)


                          gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggg                                                                          <  2:4604420/101‑1 (MQ=255)


                           cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATAACCTTGGTGCAAATATCCgggg                                                                         >  2:6339407/1‑101 (MQ=255)


                            ggggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                        >  1:6276703/2‑101 (MQ=255)


                            aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                        >  2:7269412/1‑101 (MQ=255)


                             gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                       >  1:1790557/1‑101 (MQ=255)


                               gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTg                                                                     <  2:3197857/101‑1 (MQ=255)


                               gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTg                                                                     >  1:6687696/1‑101 (MQ=255)


                                ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGTGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                                    <  2:8510735/101‑1 (MQ=255)


                                 gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                                   >  1:5116812/1‑101 (MQ=255)


                                   gcggCCCCCTCTTTCGGCTGCGCCTAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                                 <  2:5352556/98‑1 (MQ=25)


                                    cTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGcc                                                                <  1:170365/101‑1 (MQ=255)


                                    cTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCCGGATATTTGAGGCAAGATAAACGGGGGCCTATCACCTTGGTGCAAATATCCGGGGGGTGCGGcc                                                                >  2:8980923/1‑101 (MQ=38)


                                     tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                               <  2:2088743/101‑1 (MQ=255)


                                     tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                               >  2:7999021/1‑101 (MQ=255)


                                     tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                               >  2:6522584/1‑101 (MQ=255)


                                      gCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGGTATTTGAGGCAAGATAAACGGGGGCCTATCACCTTTGCGCAAAAATCCGGGGGGGGAGGCCGa                                                              >  2:38292/1‑101 (MQ=37)


                                       cccccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaa                                                             <  2:8180556/101‑1 (MQ=255)


                                        ccccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                            >  1:1144806/1‑101 (MQ=255)


                                         cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                           <  2:7148344/101‑1 (MQ=255)


                                           ctctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                                         >  2:8859464/1‑101 (MQ=255)


                                            tctTTCGGCTGCGCCGAAATTCGCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                        <  2:1244427/101‑1 (MQ=255)


                                            tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                        <  2:7385180/101‑1 (MQ=255)


                                            tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                        <  1:1020297/101‑1 (MQ=255)


                                              tttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                      <  2:5036089/101‑1 (MQ=255)


                                               ttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCAGAgg                                                     >  1:4449800/1‑101 (MQ=255)


                                                 cGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                   <  2:4085975/101‑1 (MQ=255)


                                                 cGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                   >  1:7513064/1‑101 (MQ=255)


                                                  ggCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggggg                                                  >  2:5739008/1‑101 (MQ=255)


                                                   gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                 <  2:7901735/101‑1 (MQ=255)


                                                    cTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                <  1:3786362/101‑1 (MQ=255)


                                                      gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGAGGCAgg                                              >  1:4217130/1‑100 (MQ=255)


                                                       cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                             <  1:1507080/101‑1 (MQ=255)


                                                          cGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                          <  2:8248273/101‑1 (MQ=255)


                                                           gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                         <  1:1595402/101‑1 (MQ=255)


                                                            aaaTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                        >  2:7778391/1‑101 (MQ=255)


                                                             aaTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                       >  2:2935609/1‑101 (MQ=255)


                                                                tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                    <  1:5275002/101‑1 (MQ=255)


                                                                 cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                   >  1:8028537/1‑101 (MQ=255)


                                                                 cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                   <  2:5099607/101‑1 (MQ=255)


                                                                   cccccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTccc                                 >  1:575903/1‑101 (MQ=255)


                                                                    ccccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                                >  1:1355387/1‑101 (MQ=255)


                                                                        gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                            <  1:1893538/101‑1 (MQ=255)


                                                                        gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                            >  2:2302793/1‑101 (MQ=255)


                                                                          gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                          >  1:5036949/1‑101 (MQ=255)


                                                                           atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                         >  1:6872315/1‑101 (MQ=255)


                                                                           atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                         <  1:8274432/101‑1 (MQ=255)


                                                                           atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                         >  2:6212580/1‑101 (MQ=255)


                                                                             atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                       >  1:3232791/1‑101 (MQ=255)


                                                                              tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGGAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                      >  1:5954102/1‑101 (MQ=255)


                                                                              tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                      >  1:2900228/1‑101 (MQ=255)


                                                                               ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                     >  2:871121/1‑101 (MQ=255)


                                                                               ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                     >  1:7908953/1‑101 (MQ=255)


                                                                               ttGAGGCAAGATAAACGGGCACCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                     >  1:7732585/1‑101 (MQ=255)


                                                                               atctggCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                     <  1:8694704/97‑1 (MQ=255)


                                                                                tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                    <  1:4310272/101‑1 (MQ=255)


                                                                                tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                    <  2:3157506/101‑1 (MQ=255)


                                                                                tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                    >  2:8476337/1‑101 (MQ=255)


                                                                                tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                    >  2:356592/1‑101 (MQ=255)


                                                                                  aGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                  >  1:4868432/1‑101 (MQ=255)


                                                                                    gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa                >  2:1584831/1‑101 (MQ=255)


                                                                                      aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc              <  2:9068135/101‑1 (MQ=255)


                                                                                      aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc              >  2:2628349/1‑101 (MQ=255)


                                                                                      aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc              >  2:1865991/1‑101 (MQ=255)


                                                                                        gataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt            >  1:6117130/5‑101 (MQ=255)


                                                                                        gataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt            >  1:95946/5‑101 (MQ=255)


                                                                                         ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt           <  1:6946034/98‑1 (MQ=255)


                                                                                          taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc          >  1:2652835/3‑101 (MQ=255)


                                                                                          taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc          >  2:2461434/3‑101 (MQ=255)


                                                                                           aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa         >  2:5254271/2‑101 (MQ=255)


                                                                                            aaCGGGCGCCTATCACCTTGGTGCGAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTTTCGATCGCGCACCGTTcgg        >  2:4378231/1‑99 (MQ=37)


                                                                                               gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg     >  1:1208653/1‑101 (MQ=255)


                                                                                                ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg    <  1:5678420/101‑1 (MQ=255)


GTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAT  >  NC_014034/580864‑581061

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 28 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG