Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,553 G→C 2.4% T1685S (ACC→AGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5530G→C 2.4% 4.2 ‑1.4 0.0275T1685S (ACC→AGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (146/122):  total (148/127)
Fisher's exact test strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

TCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGAT  >  NC_014034/678453‑678653
                                                                                                    |                                                                                                    
tCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCgg                                                                                                      <  2:4615341/101‑1 (MQ=255)
tCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGAGCgg                                                                                                      >  1:1768558/1‑101 (MQ=255)
 ccGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGt                                                                                                     >  1:3401416/1‑101 (MQ=255)
  cgcgCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTg                                                                                                    <  1:858/101‑1 (MQ=255)
    cgcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAc                                                                                                  >  2:2475296/1‑101 (MQ=255)
    cgcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAc                                                                                                  >  2:452928/1‑101 (MQ=255)
     gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                                                 <  2:773550/101‑1 (MQ=255)
     gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                                                 >  2:1958485/1‑101 (MQ=255)
      cAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGTGCGCGGTGAccg                                                                                                <  1:8186309/101‑1 (MQ=255)
      cAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAccg                                                                                                <  2:8253746/101‑1 (MQ=255)
           ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                                                           <  1:4811682/101‑1 (MQ=255)
           ccgccgTGCTCAGCGACGGAATCACCGCGCTGCCCAGCGCGGGGATCTGCCCGGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                                                           <  2:7137647/101‑1 (MQ=37)
              ccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTc                                                                                        >  2:7028362/1‑101 (MQ=255)
               cgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCg                                                                                       <  1:5207354/101‑1 (MQ=255)
                gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                      >  2:2630496/1‑101 (MQ=255)
                gTGGTCAGCGCAGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGAGCCGAGCCGAGCGCGGGGACCGCGGCGCTCGa                                                                                      >  2:2952097/1‑101 (MQ=38)
                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAcg                                                                                    >  2:7264372/1‑100 (MQ=255)
                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                    <  1:6121197/101‑1 (MQ=255)
                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                    <  2:6664491/101‑1 (MQ=255)
                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                    <  1:4872243/101‑1 (MQ=255)
                   gTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAg                                                                                   >  2:5969729/1‑101 (MQ=255)
                       gcgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgcc                                                                               <  1:6868539/101‑1 (MQ=255)
                         gcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccgc                                                                             >  1:3445255/1‑101 (MQ=255)
                          ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                                                            <  1:3602042/101‑1 (MQ=255)
                            ggAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAc                                                                          <  2:574362/101‑1 (MQ=255)
                             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                         >  1:3963808/1‑101 (MQ=255)
                             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                         <  1:7504892/101‑1 (MQ=255)
                             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                         <  1:221356/101‑1 (MQ=255)
                             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                         <  2:1326010/101‑1 (MQ=255)
                              aaTGACCGCGCTGCCTAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                        <  1:452448/101‑1 (MQ=255)
                              aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                        >  2:5019217/1‑101 (MQ=255)
                              aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                        >  2:7775204/1‑101 (MQ=255)
                               aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                       >  2:3213305/1‑101 (MQ=255)
                                 gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                                                     >  1:2391648/1‑101 (MQ=255)
                                 gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgac                                                                     >  1:7762948/1‑99 (MQ=255)
                                  cgggcggTGCCAATCGCCGCGACCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTgatc                                                                    <  1:6559493/98‑4 (MQ=2)
                                  aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGAGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                    >  2:4038542/1‑101 (MQ=255)
                                      cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                <  1:8249617/101‑1 (MQ=255)
                                      cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                >  1:8932950/1‑101 (MQ=255)
                                      cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                >  2:3187574/1‑101 (MQ=255)
                                       gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                               <  2:2818833/101‑1 (MQ=255)
                                       gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                               >  2:9069895/1‑101 (MQ=255)
                                        cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                              >  2:921155/1‑101 (MQ=255)
                                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                          <  1:3121596/101‑1 (MQ=255)
                                              aGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                        <  2:1502686/101‑1 (MQ=255)
                                              aGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                        >  1:3694832/1‑101 (MQ=255)
                                               gcgcCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgcc                                                       >  2:3359772/1‑101 (MQ=255)
                                                 gcggcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                     <  2:555328/98‑1 (MQ=255)
                                                 gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGGCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                     >  2:4365064/1‑101 (MQ=255)
                                                  ccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCcgcg                                                    <  1:1045924/101‑1 (MQ=255)
                                                   cgcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGa                                                   <  2:3568959/101‑1 (MQ=255)
                                                   cgcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGa                                                   >  2:878354/1‑101 (MQ=255)
                                                     cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                 >  1:4978897/1‑101 (MQ=255)
                                                     cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                 >  1:6975228/1‑101 (MQ=255)
                                                      gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                                                <  2:2284589/101‑1 (MQ=255)
                                                      gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGAGatcg                                                >  1:8077593/1‑98 (MQ=255)
                                                       aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                               <  2:4948383/101‑1 (MQ=255)
                                                        tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                              <  2:8584849/101‑1 (MQ=255)
                                                        tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                              <  1:3272194/101‑1 (MQ=255)
                                                        tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                              >  1:6292847/1‑101 (MQ=255)
                                                         cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                             <  1:6377842/101‑1 (MQ=255)
                                                         cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                             >  1:7719367/1‑101 (MQ=255)
                                                         cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                             <  1:7847883/101‑1 (MQ=255)
                                                         cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                             >  1:2727525/1‑101 (MQ=255)
                                                          tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                            >  2:370439/1‑101 (MQ=255)
                                                           gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgc                                           >  2:2353014/1‑101 (MQ=255)
                                                              ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc                                        <  2:2727443/101‑1 (MQ=255)
                                                              ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc                                        <  1:991746/101‑1 (MQ=255)
                                                                 tGCCAAATGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                     >  1:2171238/1‑101 (MQ=255)
                                                                 tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                     >  1:121095/1‑101 (MQ=255)
                                                                 ggCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                     <  1:8747920/100‑1 (MQ=255)
                                                                  gaaaacccccgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                    >  2:2044517/9‑101 (MQ=255)
                                                                   ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                   <  2:8423376/101‑1 (MQ=255)
                                                                    cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                  >  2:3299747/1‑101 (MQ=255)
                                                                    cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                  >  1:574101/1‑101 (MQ=255)
                                                                     aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                 >  1:4621000/1‑101 (MQ=255)
                                                                      aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                >  2:8094933/1‑101 (MQ=255)
                                                                      aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                >  1:8879182/1‑101 (MQ=255)
                                                                       aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                               <  2:7577243/101‑1 (MQ=255)
                                                                       aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                               <  1:5340817/101‑1 (MQ=255)
                                                                       aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                               >  2:6840883/1‑101 (MQ=255)
                                                                           cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                           >  2:5457981/1‑101 (MQ=255)
                                                                            gccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                          >  2:4839684/1‑101 (MQ=255)
                                                                            gccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                          <  2:1600818/101‑1 (MQ=255)
                                                                            gccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                          >  2:1874909/1‑101 (MQ=255)
                                                                            gccAGCAGCGCACAGCCGAGCGCGATGACCGCCGCCCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                          <  2:5183545/101‑1 (MQ=37)
                                                                             ccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATc                         <  1:2630578/101‑1 (MQ=255)
                                                                             ccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATc                         <  1:7059205/101‑1 (MQ=255)
                                                                               aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTGGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                       <  2:4721910/101‑1 (MQ=255)
                                                                                gCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgcc                      <  1:6692601/101‑1 (MQ=255)
                                                                                gCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgcc                      <  2:8896760/101‑1 (MQ=255)
                                                                                   gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                   >  2:6271970/1‑101 (MQ=255)
                                                                                      ccgagccgagCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTg                <  2:7390201/101‑1 (MQ=255)
                                                                                       cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg               <  1:5563296/101‑1 (MQ=255)
                                                                                       cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg               >  1:1931675/1‑101 (MQ=255)
                                                                                         agccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTc             <  1:4173596/101‑1 (MQ=255)
                                                                                         agccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTc             <  1:910924/101‑1 (MQ=255)
                                                                                                cgcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg      <  1:3187673/101‑1 (MQ=255)
                                                                                                  cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg    <  1:5624983/101‑1 (MQ=255)
                                                                                                    gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGac  >  2:4263962/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
TCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGAT  >  NC_014034/678453‑678653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG