Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,567 C→G 2.7% S1680S (TCG→TCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5670C→G 2.7% 8.3 ‑1.9 ‑1.3265S1680S (TCG→TCC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (143/114):  total (145/120)
Fisher's exact test strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-02

Only 100 of 279 total aligned reads displayed.

CCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCA  >  NC_014034/678467‑678667
                                                                                                    |                                                                                                    
 cgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCg                                                                                                     >  2:8591524/1‑101 (MQ=255)
  gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                    >  2:2630496/1‑101 (MQ=255)
  gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                    >  2:8249267/1‑101 (MQ=255)
  gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCGGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                    >  2:4367312/1‑101 (MQ=255)
   tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                   <  1:8097738/101‑1 (MQ=255)
   tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                   <  2:5812263/101‑1 (MQ=255)
    ggTCAGCGCCGGAATGACCGCGCTGCCCATCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                                  <  1:5797950/101‑1 (MQ=255)
    ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                                  <  1:4561297/101‑1 (MQ=255)
     gTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAg                                                                                                 >  2:5969729/1‑101 (MQ=255)
      tCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgc                                                                                                >  2:4887075/1‑101 (MQ=255)
        aGCGCCGGAGTGACCGCGCTGCCCAGCGCAGCGATCTGCGCGGTGCCAGAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgcgc                                                                                              <  2:1768505/101‑1 (MQ=255)
        aGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgcgc                                                                                              <  2:8451925/101‑1 (MQ=255)
          cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                            <  1:8923575/101‑1 (MQ=255)
           gcCGGAATGCCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccgc                                                                                           <  2:1361813/101‑1 (MQ=255)
               gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                                       <  1:221356/101‑1 (MQ=255)
               gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                                       >  2:154384/1‑101 (MQ=255)
               gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                                       >  2:1879414/1‑101 (MQ=255)
                aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                                      <  1:3232396/101‑1 (MQ=255)
                 aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                     >  2:7058920/1‑101 (MQ=255)
                  tGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgc                                                                                    >  2:1983223/1‑101 (MQ=255)
                  tGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgc                                                                                    <  1:670812/101‑1 (MQ=255)
                   gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                                                                   >  1:8793849/1‑101 (MQ=255)
                   gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                                                                   >  1:2391648/1‑101 (MQ=255)
                   gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgac                                                                                   >  1:7762948/1‑99 (MQ=255)
                    aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGAGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                                  >  2:4038542/1‑101 (MQ=255)
                        cgcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTg                                                                              <  1:360545/101‑1 (MQ=255)
                         gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                                             >  2:9069895/1‑101 (MQ=255)
                          cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                                            >  2:921155/1‑101 (MQ=255)
                           tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTc                                                                           >  1:1964293/1‑101 (MQ=255)
                           tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTc                                                                           <  2:5055634/101‑1 (MQ=255)
                            gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                          <  2:2187619/101‑1 (MQ=255)
                             gcaatcgccgcGATCGGCGCCGCGCTCAGGCCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAg                                                                         <  1:385006/96‑1 (MQ=9)
                             cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                         <  1:3135527/101‑1 (MQ=255)
                             cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                         >  1:6967619/1‑101 (MQ=255)
                             cccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAg                                                                         <  2:1001609/101‑1 (MQ=255)
                              ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                        >  1:2989792/1‑101 (MQ=255)
                              ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                        >  1:8423739/1‑101 (MQ=255)
                                atcgccgcGATCTGCCCCGCGCTCAGCGCCGCGTTCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGTCCTGATCGGTGCCCAgact                                                                      <  1:3185182/99‑4 (MQ=2)
                                 gcgcCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgcc                                                                     >  1:3467660/1‑101 (MQ=255)
                                  cgatctgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccg                                                                    <  2:8077244/95‑1 (MQ=255)
                                   gcggcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                   <  2:555328/98‑1 (MQ=255)
                                   gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGGCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                   >  2:4365064/1‑101 (MQ=255)
                                    ccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCcgcg                                                                  <  1:1045924/101‑1 (MQ=255)
                                      gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                                                                >  2:7185764/1‑92 (MQ=2)
                                       cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                               <  1:8231261/101‑1 (MQ=255)
                                       cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                               >  1:3360797/1‑101 (MQ=255)
                                       cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                               >  1:4978897/1‑101 (MQ=255)
                                        gATCTGCGCCGTGCCGAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                                                              >  2:2443364/1‑101 (MQ=255)
                                        gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                                                              <  1:6649087/101‑1 (MQ=255)
                                          tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                                            <  2:3065285/101‑1 (MQ=255)
                                           cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                                           <  1:6413667/101‑1 (MQ=255)
                                           cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                                           >  1:2727525/1‑101 (MQ=255)
                                           cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                                                           <  1:7847883/101‑1 (MQ=255)
                                            tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                          >  1:5170522/1‑101 (MQ=255)
                                            tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                          >  2:370439/1‑101 (MQ=255)
                                             gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgc                                                         >  2:2353014/1‑101 (MQ=255)
                                              cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGGGCGGTGACCGCCGCGCGCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg                                                        <  1:217569/101‑1 (MQ=255)
                                                  gTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcgt                                                    >  1:482745/1‑101 (MQ=255)
                                                  gTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcgt                                                    >  2:3396757/1‑101 (MQ=255)
                                                   tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                                   >  1:121095/1‑101 (MQ=255)
                                                   ggCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                                   <  1:7691704/100‑1 (MQ=255)
                                                   ggCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                                   <  2:3277227/100‑1 (MQ=255)
                                                    gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                                  <  2:776898/101‑1 (MQ=255)
                                                     ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                                 >  2:2319665/1‑101 (MQ=255)
                                                     ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                                 >  2:3303809/1‑101 (MQ=255)
                                                       aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                               >  1:4621000/1‑101 (MQ=255)
                                                       aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                               >  1:6135142/1‑101 (MQ=255)
                                                        aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                              <  1:4367438/101‑1 (MQ=255)
                                                         aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                             <  1:5340817/101‑1 (MQ=255)
                                                            ccgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCa                                          >  2:4990136/1‑101 (MQ=255)
                                                             cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                                         >  2:5457981/1‑101 (MQ=255)
                                                              gccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                                        <  2:6808250/101‑1 (MQ=255)
                                                              gccAGCAGCGCACAGCCGAGCGCGATGACCGCCGCCCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                                        <  2:5183545/101‑1 (MQ=37)
                                                               ccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATc                                       <  1:7059205/101‑1 (MQ=255)
                                                                cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAtgg                                      >  1:5673680/1‑99 (MQ=255)
                                                                 aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTGGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                                     <  2:4721910/101‑1 (MQ=255)
                                                                 aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                                     >  2:3197558/1‑101 (MQ=255)
                                                                  gCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgcc                                    <  2:8896760/101‑1 (MQ=255)
                                                                   cTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCATCAGCCCCGCAATcgccg                                   >  2:2795395/1‑101 (MQ=255)
                                                                    tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                                  >  2:7650104/1‑101 (MQ=255)
                                                                     gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                                 >  2:6271970/1‑101 (MQ=255)
                                                                       gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCGCGCAATCGCCGCGCt                               >  1:1799606/1‑101 (MQ=255)
                                                                       gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                               <  2:8788883/101‑1 (MQ=255)
                                                                        ccgagccgagCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTg                              <  2:7390201/101‑1 (MQ=255)
                                                                         cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                             <  1:5563296/101‑1 (MQ=255)
                                                                          gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTggc                            >  2:7513848/1‑100 (MQ=255)
                                                                          gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGt                            >  1:9157061/1‑101 (MQ=255)
                                                                           agccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTc                           <  1:4173596/101‑1 (MQ=255)
                                                                           agccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTc                           <  1:910924/101‑1 (MQ=255)
                                                                             ccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAg                         >  2:286432/1‑101 (MQ=255)
                                                                                    cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg                  <  2:6997599/101‑1 (MQ=255)
                                                                                    cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg                  >  1:6342683/1‑101 (MQ=255)
                                                                                      gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGac                >  2:4263962/1‑100 (MQ=255)
                                                                                       tGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATc               >  1:7525076/1‑101 (MQ=255)
                                                                                             ccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGcc         <  2:574091/101‑1 (MQ=255)
                                                                                               gcgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGt       <  2:2701583/101‑1 (MQ=255)
                                                                                                cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg      <  1:4887235/101‑1 (MQ=255)
                                                                                                 gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg     >  1:7477775/1‑101 (MQ=255)
                                                                                                 gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg     >  1:368673/1‑101 (MQ=255)
                                                                                                   tCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTc   <  1:5854915/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCA  >  NC_014034/678467‑678667

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG