Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,783 G→C 6.7% S1608R (AGC→ACC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,7830G→C 6.7% 49.4 ‑0.6 ‑0.2385S1608R (AGC→AGG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (16/10):  ref base (194/164):  total (210/175)
Fisher's exact test strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.30e-01

Only 100 of 413 total aligned reads displayed.

CCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCA  >  NC_014034/678683‑678883
                                                                                                    |                                                                                                    
 cGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATAGCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGc                                                                                                     >  2:2880852/1‑101 (MQ=255)
   tGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTc                                                                                                   <  1:8504793/101‑1 (MQ=255)
    gCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                                                  >  1:1671140/1‑101 (MQ=255)
    gCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                                                  <  2:7759016/101‑1 (MQ=255)
    gCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                                                  <  1:5216699/101‑1 (MQ=255)
     cTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAg                                                                                                 <  2:368665/101‑1 (MQ=255)
       cAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                                                                               >  2:1864275/1‑101 (MQ=255)
       cAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                                                                               <  2:5451709/101‑1 (MQ=255)
        aGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                                                                              >  1:1174268/1‑101 (MQ=255)
          gCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCCGCGCCGCGATCTGCGCCGTGCTCAGcgccc                                                                                            >  1:6434109/1‑100 (MQ=255)
          gCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                                                            >  1:1767172/1‑101 (MQ=255)
           cTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                                                           >  2:841511/1‑101 (MQ=255)
           cTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                                                           >  1:2854520/1‑101 (MQ=255)
            ttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCcgcg                                                                                          >  1:3451460/1‑101 (MQ=255)
               gCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATc                                                                                       <  1:5913243/101‑1 (MQ=255)
                cagcatcgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCt                                                                                      <  2:5935004/95‑1 (MQ=255)
                 aTCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTg                                                                                     >  1:8767192/1‑101 (MQ=255)
                   cAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCg                                                                                   >  1:1515621/1‑101 (MQ=255)
                    aGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGt                                                                                  <  1:988806/101‑1 (MQ=255)
                      cgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcg                                                                                >  2:1037509/1‑101 (MQ=255)
                      cgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcg                                                                                <  1:8351539/101‑1 (MQ=255)
                           atctgATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                                                           <  1:2356420/97‑1 (MQ=255)
                            cccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCa                                                                          >  1:6268906/1‑101 (MQ=255)
                             ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                                                         <  2:6674258/101‑1 (MQ=255)
                               gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAgat                                                                       >  2:2356353/1‑99 (MQ=255)
                               gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                                                       <  1:550872/101‑1 (MQ=255)
                                aTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                                                      >  1:4123694/1‑101 (MQ=255)
                                 atcccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                     <  2:1548989/99‑1 (MQ=255)
                                  ccccGCCATGGCCGAGACCGAAATCTGGGCGATATCCGAGGTTTCCAGAGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                    >  1:7482792/1‑101 (MQ=255)
                                  ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                    <  2:6062951/101‑1 (MQ=255)
                                  ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                    >  1:4893894/1‑101 (MQ=255)
                                     ccccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                 <  2:1306914/99‑1 (MQ=255)
                                      gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                                                >  1:3391963/1‑101 (MQ=255)
                                      gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                                                >  2:3617749/1‑101 (MQ=255)
                                       ccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGGTTCCAGCGCCCCGAACTGCGCCGTGCTCAGCGCCGCGGTCTGCGTCGCGGTCAGCCCGgccgcc                                                               >  2:7982855/1‑101 (MQ=37)
                                          tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                            >  2:3397377/1‑101 (MQ=255)
                                            gCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcgc                                                          >  1:3287898/1‑101 (MQ=255)
                                             ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGcc                                                         <  1:2663962/101‑1 (MQ=255)
                                              cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                        >  2:6422477/1‑101 (MQ=255)
                                               gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                       <  1:7028647/101‑1 (MQ=255)
                                               gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                       >  1:448828/1‑101 (MQ=255)
                                                agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTg                                                      <  2:4411501/101‑1 (MQ=255)
                                                   ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                                                   >  2:8873798/1‑101 (MQ=255)
                                                    cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                  <  1:2554496/101‑1 (MQ=255)
                                                     gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                                                 >  2:3957929/1‑101 (MQ=255)
                                                      aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                <  1:3780855/101‑1 (MQ=255)
                                                      aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                >  2:2517182/1‑101 (MQ=255)
                                                      aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                <  2:4655374/101‑1 (MQ=255)
                                                      aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                >  1:4353137/1‑101 (MQ=255)
                                                       aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                               >  2:4286789/1‑101 (MQ=255)
                                                           tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                           <  2:3108296/101‑1 (MQ=255)
                                                           tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                           <  2:6852841/101‑1 (MQ=255)
                                                           tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                           >  2:7888919/1‑101 (MQ=255)
                                                             caccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                         >  2:4764651/7‑101 (MQ=2)
                                                                gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt                                      >  1:7306284/1‑101 (MQ=255)
                                                                 agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                     <  2:7506741/99‑1 (MQ=2)
                                                                 agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                     <  1:8121547/99‑1 (MQ=2)
                                                                  tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                    <  1:3958044/101‑1 (MQ=255)
                                                                  tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                    <  2:730778/101‑1 (MQ=255)
                                                                  gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                    >  1:7947891/2‑101 (MQ=2)
                                                                   atCGGCGGTTTCCAGCGCCGCACCCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCCCCCCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                   <  1:2986841/101‑1 (MQ=2)
                                                                   atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                   <  1:2063183/101‑1 (MQ=2)
                                                                   atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt                                   <  2:8603490/101‑1 (MQ=255)
                                                                    ccggcgGGTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAtt                                  <  2:1165555/98‑1 (MQ=255)
                                                                     ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc                                 <  1:1983283/101‑1 (MQ=255)
                                                                      cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                <  2:5170353/101‑1 (MQ=255)
                                                                      cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                <  2:7581350/101‑1 (MQ=255)
                                                                      cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                >  1:3180747/1‑101 (MQ=255)
                                                                       gcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGt                               >  2:277656/1‑101 (MQ=255)
                                                                        cgGTTTCCAGCCCCGCAGCCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAtcggtg                              <  2:1211300/101‑6 (MQ=9)
                                                                         ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                             >  1:8669738/1‑101 (MQ=255)
                                                                           tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                           <  1:1037527/101‑1 (MQ=255)
                                                                           tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGATCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                           >  2:7133974/1‑101 (MQ=255)
                                                                            ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                          >  2:1972617/1‑101 (MQ=255)
                                                                             tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTTCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                         >  1:4735574/1‑101 (MQ=255)
                                                                             tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                         <  1:3526542/101‑1 (MQ=255)
                                                                             tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                         >  1:6180509/1‑101 (MQ=255)
                                                                              ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGc                        <  2:8054081/101‑1 (MQ=255)
                                                                              ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGc                        >  2:7097178/1‑101 (MQ=255)
                                                                               cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGcc                       <  2:4980696/101‑1 (MQ=255)
                                                                                aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                      >  2:3522613/1‑101 (MQ=255)
                                                                                aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                      >  1:4598105/1‑101 (MQ=255)
                                                                                aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                      >  2:4553881/1‑101 (MQ=255)
                                                                                   gccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCtt                   <  2:1686122/101‑1 (MQ=255)
                                                                                    ccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTc                  >  2:6168310/1‑101 (MQ=255)
                                                                                     cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                 <  1:9058399/101‑1 (MQ=255)
                                                                                       cgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgc               <  1:2203637/101‑1 (MQ=255)
                                                                                       cgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgc               <  1:6768397/101‑1 (MQ=255)
                                                                                        gATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcg              >  2:8745132/1‑101 (MQ=255)
                                                                                         aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc             <  2:4878688/101‑1 (MQ=255)
                                                                                         aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc             <  1:1389645/101‑1 (MQ=255)
                                                                                          tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc            <  2:5667720/101‑1 (MQ=255)
                                                                                            tGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccgc          >  2:2098557/1‑101 (MQ=255)
                                                                                             gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg         <  1:7662739/101‑1 (MQ=255)
                                                                                                ccGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTg      <  2:5313255/101‑1 (MQ=255)
                                                                                                ccGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTg      <  2:3803563/101‑1 (MQ=255)
                                                                                                 cGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGc     >  2:2240538/1‑101 (MQ=255)
                                                                                                   tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc   <  1:8454722/101‑1 (MQ=255)
                                                                                                    gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa  <  1:5822938/101‑1 (MQ=255)
                                                                                                    gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa  <  2:8480696/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCA  >  NC_014034/678683‑678883

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG