Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,129 A→G 35.8% D826D (GAT→GAC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,1290A→G35.8% 165.6 ‑3.1 ‑0.2168D826D (GAT→GACRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (43/16):  ref base (60/47):  total (104/64)
Fisher's exact test strand distribution p-value = 4.45e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.71e-01

Only 100 of 179 total aligned reads displayed.

TCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCG  >  NC_014034/681031‑681229
                                                                                                  |                                                                                                    
tCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCaga                                                                                                    >  2:6089523/1‑99 (MQ=255)
   tcgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGt                                                                                                 >  1:1460138/1‑101 (MQ=255)
    cgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtc                                                                                                <  2:6432080/101‑2 (MQ=255)
    cgacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtc                                                                                                >  2:3852077/1‑100 (MQ=255)
     gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                               >  1:6225004/1‑99 (MQ=255)
     gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                               <  1:3352003/101‑3 (MQ=255)
     gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                               >  2:5256707/1‑99 (MQ=255)
     gacctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcg                                                                                               >  1:1924313/1‑99 (MQ=255)
      acctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcga                                                                                              <  1:8148172/101‑4 (MQ=255)
        ctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgaca                                                                                            <  2:162346/101‑6 (MQ=255)
        ctcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgaca                                                                                            <  2:1981758/101‑6 (MQ=255)
         tcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGTGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacag                                                                                           <  1:1035628/101‑7 (MQ=38)
         tcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGCTGTCGCCGGtcgacag                                                                                           >  2:5865606/1‑95 (MQ=38)
         tcgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacag                                                                                           >  1:4441534/1‑95 (MQ=38)
          cgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagc                                                                                          >  1:1678166/1‑94 (MQ=38)
          cgaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagc                                                                                          <  1:8498080/101‑8 (MQ=38)
           gaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagcc                                                                                         >  1:1414371/1‑93 (MQ=37)
           gaTCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGtcgacagcc                                                                                         >  1:4819483/1‑93 (MQ=37)
                     aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                               >  1:3907128/1‑101 (MQ=25)
                     aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                               >  1:9038010/1‑101 (MQ=25)
                      gCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcg                                                                              >  2:3280694/1‑100 (MQ=25)
                       cTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgc                                                                             >  2:7443536/1‑99 (MQ=25)
                       cTGCGCCGTGCCCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGTCAGCCCCTTGAGCGcgc                                                                             >  2:3695744/1‑99 (MQ=21)
                        tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                            >  1:3323110/1‑98 (MQ=25)
                         gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                                           >  2:210373/1‑97 (MQ=25)
                         gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                                           >  1:2475873/1‑97 (MQ=25)
                           gcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccact                                                                         >  2:4935871/1‑95 (MQ=25)
                           gcCGTCCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATTTCTCCGGTCGACAGCCCCTTGAGCGcgccact                                                                         <  1:2889766/101‑7 (MQ=18)
                             cGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactgg                                                                       >  1:8926874/1‑93 (MQ=21)
                              gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                                      >  1:1161173/1‑92 (MQ=21)
                               tGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggcc                                                                     >  2:148437/1‑91 (MQ=255)
                               tGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggcc                                                                     >  2:8768534/1‑91 (MQ=255)
                                                                         gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                           >  2:7677701/9‑101 (MQ=38)
                                                                         gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                           <  2:8623053/93‑1 (MQ=255)
                                                                          cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                          <  1:5025646/94‑1 (MQ=255)
                                                                           gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt                         >  1:5694987/7‑101 (MQ=255)
                                                                           gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt                         <  2:7903390/95‑1 (MQ=255)
                                                                            ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                        >  1:4932197/6‑101 (MQ=255)
                                                                            ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                        >  2:8628721/6‑101 (MQ=255)
                                                                            ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                        >  2:2259185/6‑101 (MQ=255)
                                                                             cgtggTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                       >  1:7227767/4‑101 (MQ=255)
                                                                             cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                       <  2:510144/97‑1 (MQ=255)
                                                                             cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                       >  1:1120938/5‑101 (MQ=255)
                                                                              gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                      <  2:8484766/98‑1 (MQ=255)
                                                                               tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                     <  1:4195428/99‑1 (MQ=255)
                                                                               tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                     <  2:2670400/99‑1 (MQ=255)
                                                                               tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                     <  2:2785426/99‑1 (MQ=255)
                                                                                cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                    >  2:2299381/2‑101 (MQ=255)
                                                                                cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                    <  1:993960/100‑1 (MQ=255)
                                                                                cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCGGCGTCGTGCTCa                    <  1:8187077/100‑1 (MQ=255)
                                                                                 gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                   >  1:3202674/1‑101 (MQ=255)
                                                                                 gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                   <  2:7369027/101‑1 (MQ=255)
                                                                                 gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                   <  1:6781315/101‑1 (MQ=255)
                                                                                 gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                   <  2:8699347/101‑1 (MQ=255)
                                                                                  gcAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc                  <  2:6986677/100‑1 (MQ=255)
                                                                                   cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                 >  1:3089613/1‑101 (MQ=255)
                                                                                   cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                 >  2:4876155/1‑101 (MQ=255)
                                                                                   cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                 >  1:3574361/1‑101 (MQ=255)
                                                                                   cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                 >  2:4708534/1‑101 (MQ=255)
                                                                                   cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                 >  1:5842110/1‑101 (MQ=255)
                                                                                    aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                <  2:1786384/101‑1 (MQ=255)
                                                                                     gcgcCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGcgcg               <  1:8732160/101‑1 (MQ=255)
                                                                                     gcgcCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGcgcg               >  2:8379706/1‑101 (MQ=255)
                                                                                      cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg              >  2:1573099/1‑101 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc             >  1:5312161/4‑101 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc             >  1:2414597/4‑101 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc             <  1:6810495/98‑1 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc             >  1:8233156/4‑101 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc             >  1:4325319/4‑101 (MQ=255)
                                                                                       gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGGGCTCCGCGCGGc             >  2:9043788/4‑101 (MQ=255)
                                                                                        ccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTTCTCAGCGCGGcc            <  1:6528621/99‑1 (MQ=255)
                                                                                         cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa           >  2:1364915/2‑101 (MQ=255)
                                                                                         cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa           <  1:4530063/100‑1 (MQ=255)
                                                                                           cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc         >  2:2779217/1‑101 (MQ=255)
                                                                                           cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc         >  2:7795803/1‑101 (MQ=255)
                                                                                           cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc         >  1:152676/1‑101 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        >  1:5012736/1‑101 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        <  1:747607/101‑1 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        >  2:8265089/1‑101 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        <  1:7491438/101‑1 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        <  1:4736802/101‑1 (MQ=255)
                                                                                            gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt        <  1:127490/101‑1 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       <  1:5142070/101‑1 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       >  1:6627590/1‑101 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       <  1:7917828/101‑1 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       <  1:915697/101‑1 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       <  2:2542709/101‑1 (MQ=255)
                                                                                             aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       >  2:450731/1‑101 (MQ=255)
                                                                                             aTGGCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg       <  2:7554991/101‑1 (MQ=255)
                                                                                              tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc      <  2:3987261/101‑1 (MQ=255)
                                                                                              tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc      >  2:3265669/1‑101 (MQ=255)
                                                                                               gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg     >  2:181476/1‑101 (MQ=255)
                                                                                                tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCACGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc    >  1:5976422/1‑101 (MQ=255)
                                                                                                tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc    <  2:5893738/101‑1 (MQ=255)
                                                                                                tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc    <  1:7374132/101‑1 (MQ=255)
                                                                                                 cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc   >  2:7858348/1‑101 (MQ=255)
                                                                                                 cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGAGcc   >  1:1806788/1‑101 (MQ=255)
                                                                                                  aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg  >  1:2055461/1‑101 (MQ=255)
                                                                                                  aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg  <  1:393316/101‑1 (MQ=255)
                                                                                                  aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg  >  2:4759021/1‑101 (MQ=255)
                                                                                                  |                                                                                                    
TCCTCGACCTCGATCGCCGCCAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCG  >  NC_014034/681031‑681229

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG